... with 10 % (v/v) foetal bovine serum, glucose (4.5 gÆL )1) , streptomycin sulfate ( 10 0 mgÆL )1) , penicillin G ( 10 0000 UÆL )1) , uridine ( 50 mgÆL )1) and pyruvate ( 10 0 mgÆL )1) unless otherwise stated ... Plasmid DNA ( 10 0 lgÆmL )1; 6.8 kb) was incubated for h at 30 °C with 0 10 00 lM mitoDC- 81 In (C) and (D) rat liver mitochondria (1 mg proteinÆmL )1) were incubated for h at 30 °C with lM mitoDC- 81 after ... permeabilized and blocked with 10 % (v/v) foetal bovine serum, 0 .1% (v/v) Triton X - 10 0, NaCl/Pi for 10 at °C before being shaken overnight at °C with a : 500 dilution of rabbit anti-TPP serum and a : 10 0...
... mitochondrion Bars ¼ 200 nm 10 88 FEBS Journal 274 ( 200 7) 10 83 10 92 ª 200 7 The Authors Journal compilation ª 200 7 FEBS A Martelli et al producing m-hIRP1 established that a large proportion of the human ... structural and FEBS Journal 274 ( 200 7) 10 83 10 92 ª 200 7 The Authors Journal compilation ª 200 7 FEBS 10 89 Human IRP1 forms aggregates in yeast mitochondria A Martelli et al functional properties of mitochondrial ... details of the ‘iron–sulfur switch’ regulating this protein in metazoan cells Experimental procedures Strains and media Strains W 303 -1B (mat a, ade2 1, ura3 1, his3 11 , trp1 1, leu2–3 ,11 2, can1 10 0) ,...
... There were 11 6 reports of Clouded Apollo from 19 03 to 200 2 in Estonia Most of them (85; 73%) are from the period post -19 90, in the 19 80- s there were 12 ( 10 %) and from the earlier period 19 (16 %) records ... Fred 1, Robert B O’Hara2 & Jon E Brommer1 University of Helsinki, Department of Bio- and Environmental sciences, P.O Box 65 (Viikinkaari 1) , FIN 0001 4 , Finland Department of Mathematics and Statistics, ... between the federal states of North Rhine-Westphalia and Hesse ( 51 22’N/8°38’E and 51 38’N/9°25’E) at an elevation of 10 0 to 6 10 m a.s.l The climate is suboceanic and varies greatly according to altitude...
... 12 10 . Certificate Application 12 10 .1 Documentation Requirements 12 10 .2 Certificate Request 12 10 .2 .1 General 12 10 .2.2 ... later; A national scheme that audits conformance to ETSI TS 10 1 456 v1.2 .1 or later; A national scheme that audits conformance to ETSI TS 10 2 04 2 V1 .1. 1 or later; A scheme that audits conformance ... Certificates Validity period beginning on or before 31 Dec 2 010and ending on or before 31 Dec 2 01 3 Validity period beginning after 31 Dec 2 010 or ending after 31 Dec 2 01 3 Digest algorithm SHA -1, ...
... positive constants 1and C such that 1 b(t) ≤ C + C |t| for t ∈ R (H1 ) 1 q1+ < 1 p− , q2+ < 1 p− Then the functional E is coercive and attains its infimum in X at some u0 ∈ X Therefore, u0 is ... 3 .1 Let (f1 ), (g1 ), (a1 ) and (b1 ) hold Then the following statements hold true: (1) a ∈ C ( [0, ∞)) ∩ C ( (0, ∞)), a (0) = 0, a (t) = a(t) > 0; b ∈ C (R), b (0) = (2) J, Φ, Ψ and E ∈ C (X), J (0) ... a satisfies (a1 ) may be singular at t = And f , g satisfying (f1 ) f : Ω × R → R satisfies the Caratheodory condition and there exist two constants C1 ≥ 0, C2 ≥ such that C1 + C2 |t|q1 (x) 1 ,...
... Appl Math 50, 703 – 715 (19 90) [ 10 ] Pei, R: Multiple solutions for biharmonic equations with asymptotically linear nonlinearities Bound Value Probl 2 010 , Article ID 2 415 18, 11 (2 010 ) 17 ... ρ such that I ± (e) < Define P = {γ : [0, 1] → H : γ is continuous and γ (0) = 0, γ (1) = e}, 14 and c± = inf max I ± (γ(t)) γ∈P t∈ [0 ,1] From Lemma 3.3, we have I ± (0) = 0, I ± (e) < 0, I ± (u)|∂Bρ ... corresponding eigenfunctions of1 From ( 10 ), we get ∆ω∆ 1 dx + m Ω ω + 1 dx ω 1 dx = β Ω (13 ) Ω On the other hand, we can easily see that Λ = 1 ( 1 + m ) is the eigenvalue of the problem ∆...
... Applications, vol 47, no 7, pp 47 31 4742, 20 01 15 Z He and J Yu, “On the existence of positive solutions of fourth-order difference equations,” Applied Mathematics and Computation, vol 16 1, no 1, ... 583–5 91, 200 2 17 J Yu and Z Guo, “On boundary value problems for a discrete generalized Emden-Fowler equation,” Journal of Differential Equations, vol 2 31, no 1, pp 18 – 31, 200 6 18 L Jiang and Z ... Applied Mathematics and Computation, vol 203 , no 1, pp 30 38, 200 8 20 D Bai and Y Xu, “Nontrivial solutions of boundary value problems of second-order difference equations,” Journal of Mathematical...
... n 1 φ x1 t1 n−2 ≥ f t1 , β t1 , , β n 1 t1 n 1 , x1 t1 x1 t1 t1 − f t1 , β t1 , , β n 1 t1 n 1 t1 − β n−2 t1 φ x1 3. 40 t1 − f t1 , β t1 , · · · , β n 1 t1 n−2 x1 Case t1 n 1 t1 − β n−2 t1 ... that x0 t0 > or x0 t0 < We may assume x0 t0 > There exists t1 ∈ a, c such that n−2 max x0 t∈ a,c n 1 Then x0 t1 n 0, x0 n−2 t : x0 t1 > 2.8 t1 ≤ From 2.6 we have n ≥ x0 t1 n−2 x0 n 1 t1 φ x0 t1 ... x1 t1 β n 1 t1 n and x1 t1 ≤ β n t1 By conditions i and ii , we get the following contradiction: n ≥ x1 t1 − β n t1 n−2 ≥ f t1 , w0 t1 , x1 t1 , , wn−2 t1 , x1 n−2 x1 n−2 t1 − wn−2 t1 , x1...
... Yuan et al 11 Acknowledgments The work was supported by the National Natural Science Foundation of China Grants no 10 5 7 10 21 and 10 7 010 20 The work was supported by Subject Foundation of Harbin ... difference equation,” Journal of Inequalities in Pure and Applied Mathematics, vol 6, no 1, Article 10 , pp 1 11 , 200 5 11 P J Y Wong and R P Agarwal, “On the existence of solutions of singular boundary ... t ∈ 0, , x > 0; for t ∈ 0, , x > 4 . 10 g tx ≥ t g x , g t ≥ t g , α Let t for t ∈ 0, , x > α 4 .11 1/ x, x > 1, one has g x ≤ xα g , Let e i K i, i / T 4 .12 , and we define x∈P | Qe for x ≥ 1 e i...
... Yuan et al 11 Acknowledgments The work was supported by the National Natural Science Foundation of China Grants no 10 5 7 10 21 and 10 7 010 20 The work was supported by Subject Foundation of Harbin ... difference equation,” Journal of Inequalities in Pure and Applied Mathematics, vol 6, no 1, Article 10 , pp 1 11 , 200 5 11 P J Y Wong and R P Agarwal, “On the existence of solutions of singular boundary ... t ∈ 0, , x > 0; for t ∈ 0, , x > 4 . 10 g tx ≥ t g x , g t ≥ t g , α Let t for t ∈ 0, , x > α 4 .11 1/ x, x > 1, one has g x ≤ xα g , Let e i K i, i / T 4 .12 , and we define x∈P | Qe for x ≥ 1 e i...