dendritic cells as keepers of peripheral tolerance
Báo cáo y học: "Myeloid dendritic cells display downregulation of C-type lectin receptors and aberrant lectin uptake in systemic lupus erythematosus" pdf
... percentage of cells staining positive for different markers as well as by mean channel fluorescence The cutoff point for positive staining was above the level of the control isotype antibodies IFNγ measurement ... monocyte-derived dendritic cells display decreased FITC-dextran uptake (a) Unstimulated dendritic cells (DCs) (*P = 0.003) (b) Lupus monocyte-derived dendritic cells display decreased FITC-dextran ... differentiation, increased baseline maturation, augmented synthesis of proinflammatory cytokines, and increased ability to promote increased proliferation and activation of allogeneic control T cells [21]...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... use of both moDC and KG1 cells as mDC models, their similarity to peripheral blood DCs is yet to be properly defined This study aims to assess the suitability of both moDC and KG1 cells as model ... cells [31,32] and other cells of the innate immune system (for example, natural killer cells) , as well as in exerting Genome Biology 2007, 8:R30 information Our finding of negligible levels of ... digestion and MALDI-TOF (matrix-assisted laser desorption/ionisation-time of flight) mass spectrometric analysis; Table shows the corresponding protein data The factor of difference was calculated by...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... a range of concentrations (0–15 lM, as indicated) (A,B) GFP was detected by FACS analysis; cells were gated as GFP-positive based on fluorescence of uninfected cells, and the percentage of GFP ... (filled) cells The gate shown represents GFP-positive cells as determined by fluorescence of uninfected cells Some 43.7% of CD40 transfected cells were determined to be GFP positive vs 12.6% of mock-transfected ... unmodified AdV-GFP was virtually undetectable even at an m.o.i of 2600 (% GFP positive cells was 0.2%, identical to the background level of fluorescence measured as in the absence of added adenovirus)...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... degradation of endocannabinoids occurs via the fatty acid amide hydrolase (FAAH) [30] in neuronal as well as immune cells, such as RBL-2H3 basophilic leukemia cells [22], U937 monocytic cells [31], ... modulation of CB1, CB2 and FAAH mRNA expression in immature vs mature dendritic cells, we compared the expression of these genes by RT-PCR in immature dendritic cells, used as controls, with that of dendritic ... representative of all the samples analyzed, as in only three preparations out of the six analyzed was a decrease of mRNA transcripts observed spleen used as a positive control This band, whose staining was...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... produced MHz ultrasound Treatment apparatus A Plexiglas cylinder was used as the ultrasound chamber (70 mm diameter, 25 mm tall) The bottom of this chamber was a single layer of acoustically transparent ... against the bottom of the ultrasound chamber during treatment Ultrasound gel was used to coat the transducer face and the underside of the latex sheet used as the bottom of the ultrasound chamber ... A ring of ultrasound absorbing material was suspended cm from the bottom of the treatment chamber to aid positioning of the testes and to reduce reflection of ultrasound energy An ultrasound-transparent,...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... reduction of the apparent molecular mass to 25 kDa in immature as well as in mature dendritic cells, indicating identical N-linked glycosylation of CD63 in immature as well as mature dendritic cells ... maturation of dendritic cells, the apparent molecular mass of each of these eight carbohydrate containing polypeptides was increased by 10 kDa (Fig 2B) In mature dendritic cells, the 34 kDa form of ... protein, the molecular mass of which increased to 70 kDa after maturation of the cells induced by LPS (Fig 1A, lane and 4) The increase in molecular mass of CD63 was independent of the stimulus used...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... by washes with Perm/Wash Buffer The percentage of cytotoxicity (mean ± SD of replicates) was determined by the following calculation: percentage of Caspase-3 staining = [(Caspase-3+PKH-26+ cells) /(Caspase-3+ ... cytometry CTL assays that were predicated on measurement of CTL-induced caspase-3 activation in target cells through detection of specific cleavage of fluorogenic caspase-3 [36,37] The fusion cells could ... CTLinduced caspase-3 activation in the target cells through detection of the specific cleavage of fluorogenic caspase-3 using Active Caspase-3 Apoptosis Kit I (BD Pharmingen) [36,37] The target cells...
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báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
... of the stimulated T -cells K562 cells were used as targets to observe natural killer (NK) cell activity In all of these CTL killing assays, spontaneous release of chromium never exceeded 25% of ... plasmids ins96-0.8 or pSH3, using HEK293 cells as described previously [28,30,32] Lysates of HEK293 cells were used as virus-negative controls for mock infections Immunofluorescence HEK293 cells ... multiplicity of infection of 100, days before the 51Cr release assay Lipofection using DOTAP The recombinant IE1 protein was made as previous described [33] Lipofection was performed using the cationic...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... was at least as efficient as an ubiquitous promoter (data not shown) In contrast to the desmin promoter, the C512 promoter was also active in monocyte-derived DC and LC (around 10% of E-GFP+ cells) ... days of co-culture, percentages of CD8+ dividing T -cells measured by flow cytometry were linearly correlated with the loss of CFSE fluorescence The data in panel A are shown as the mean of triplicate ... pathway in vaccinia virus-infected cells for presentation of cytoplasmic antigens to class II-restricted T cells J Exp Med 1990, 172:947-954 Chinnasamy N, Chinnasamy D, Toso JF, Lapointe R, Candotti...
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báo cáo hóa học:" yuDetecting the percent of peripheral blood mononuclear cells displaying p-STAT-3 in malignant glioma patients" pot
... STAT-3 has been found to be constitutively activated in 50-90% of all malignant tumors, including 53% of anaplastic astrocytomas and 53% of glioblastomas [3] In gliomas, cytokines, such as interleukin ... clinical trial testing A limitation of this assay is that an increase in the mean percentage of PBMCs displaying p-STAT-3 was not detected in all cases of malignant gliomas; however, an elevation in ... analysis Determination of Tregs in peripheral blood of patients withgliomas For subset analysis, after we became proficient at analyzing PBMC p-STAT-3, after the isolation of PBMCs as described above,...
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Báo cáo hóa học: " Molecular signatures of maturing dendritic cells: implications for testing the quality of dendritic cell therapies" pptx
... design of the probe set was based on the Ensemble Human Database build (NCBI-35c), with full coverage of the NCBI human Reference sequence dataset (April 2, 2005) The microarray was composed of 48 ... These Treg cells can counter the effects of Th1 responses by cytotoxic T cells, Th1 cells, and NK cells In contrast, we found that LPS and IFN-g maturated DCs did not increase the levels of CCL22 ... Moreover, the expression of Oncostatin M (OSM), which enhances the expression of the CCR7 ligand CCL21 by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
... disease progression Study Assessments To evaluate the primary objective of clinical activity, disease assessment was performed at baseline and in weeks and 12 Tumor growth control was expressed as ... total of 12 vaccinations with a best response of SD; the therapy resulted in a significant arrest of growth of the lung metastasis The patient underwent surgical resection of all active sites of ... at last follow-up at 24+ months Patient 095-060 was enrolled with lung metastases of a mucosal melanoma of nasal primary, without previous systemic treatment The patient received a total of 10...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
... trend of increase of the expression of HLA-DR (Figure 6D) No significant increase of these molecules was detected by cytofluorimetric analysis in the CD14+ monocytes isolated from the group of subjects ... analysis was performed by FlowJo software (Tree-Star), excluding dead cells and including cells falling in the expected morphological gate The band pass filter used for cytofluorimetric analysis was ... to the transcripts profiling of cycle 1, the unsupervised hierarchical clustering of the complete database, based on the expression of these 179 genes obtained from cycle class comparison analysis,...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein tyrosine kinases ... was used as a standard measure of proliferation [41] During the final 16 hours of culture, the cells were pulsed by adding µCi 3[H]-thymidine to each well Cells were harvested and the uptake of ... inability of a tumor antigen to induce high avidity T -cells [30,31] and possibly most significant and general of all, tumor cells commonly down-regulate expression of either class I or class II...
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báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt
... Expression of NgRs in human and mouse DCs As our aim was to expand on current knowledge of the role of NgRs in non-CNS cells, we began the study as a screen for NgR1 expression in human peripheral ... immature dendritic cells (immDCs) Addition of a defined maturation cocktail for the last two days of culture resulted in generation of mature DCs (matDCs) Maturation cocktail (MC) consisted of interleukin ... flasks After two days, flasks were gently swirled and 75% of medium was removed The same volume of fresh medium was added back, containing 40 ng/ml GM-CSF On day 4, the culture is made up of...
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báo cáo hóa học:" Comparative study of clinical grade human tolerogenic dendritic cells" potx
... cells as shown in Figure 6A Then, as depicted, only those T cells stimulated by Rapa-DCs showed a significantly increase of the percentages of CD4+ Foxp3+ and CD25 high , CD127 low/negative cells ... Small (solid line) non-blast cells and large (dotted line) blast cells are circled (A) Phenotype of T cells as CD4+, Foxp3+ and CD25+ with low or null CD127 expression One of representative experiments ... DC-tols not promote a Th1 profile To analyze the effect of the different tol-DCs, allostimulated T cells were further studied An example of the Page of 14 proliferation of T cells allostimulated by...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein tyrosine kinases ... was used as a standard measure of proliferation [41] During the final 16 hours of culture, the cells were pulsed by adding µCi 3[H]-thymidine to each well Cells were harvested and the uptake of ... inability of a tumor antigen to induce high avidity T -cells [30,31] and possibly most significant and general of all, tumor cells commonly down-regulate expression of either class I or class II...
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