core ns3 ns4b and ns5a proteins form the major immunogenic proteins of hcv virus
Báo cáo khoa học: "Development of a RVFV ELISA that can distinguish infected from vaccinated animals" pps
... assisted in the design of the study and the molecular cloning AKM performed the cloning, gene expression, purification, immunoassays and drafted the manuscript STN conceived of the study and participated ... control sera confirmed the specificity of the assay and secondary only controls demonstrated the low level of background in these assays Analysis of the antibody response in rats and demonstration ... that they are preceded by epizootics in livestock These livestock epizootics serve as an amplification step in the spread of the virus Prevention of disease in animals through the use of a safe and...
- 11
- 279
- 0
Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
... Y(t) ¼ e–kt · 100%, where Y is the percentage of the linear form, and t is the refolding time For the rate of increase of the native form in the presence of cPDI, the original data were fitted by ... Ymax(1 ) e–kt ) · 100%, where Y is the percentage of the native form, and t is the refolding time For the rate of increase of the native form in the absence of cPDI, the original data were fitted by ... the rate of increase of the native form, the original data were fitted by Y(t) ¼ Ymax(1 ) e–kt) · 100%, where Y is the percentage of the native form, and t is the refolding time For the rate of...
- 10
- 405
- 0
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... species; reduction and alkylation, and mutation of the Cys implicated in the intermolecular disulfide bridge Analysis of the three-dimensional structure of SSA [31] to determine which of the remaining ... treatment of several pathologies and because of the potential use of the toxins as biological weapons Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the...
- 9
- 485
- 0
Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
... PCR at either the 5¢-end or 3¢-end of the coding regions of the two HRT cDNAs The resulting fragments were sequenced, digested with NcoI and BamHI, and ligated into the NcoI–BamHI vector of pET32b(+), ... pET32b(+), yielding the expression plasmids pETHRT1 and pETHRT2 Each of the expression plasmids was used for transformation of E coli BL21(DE3), and mL of an overnight culture of the transformant in Luria–Bertani ... Overexpression of HRT1 and HRT2 in E coli and purification of HRT2 The pETHRT1 and pETHRT2 were constructed and introduced into E coli BL21(DE3) The expression of these genes were induced by addition of 0.5...
- 10
- 516
- 0
Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases docx
... elucidate the dose-dependence of the regulation of the activity of the zebrafish ST by these divalent metal cations and their modes of action Fig Effects of divalent metal cations on the sulfating ... dopamine and T3, whereas zebrafish SULT1 ST2 was more active toward the thyroid hormones (T3 and T4), estrone, and dopa Whether these activities reflect truly the physiological functions of the two ... optima of the ZF SULT1 ST2 correspond to two distinct conformational states of the enzyme remains to be clarified A number of endogenous and xenobiotic compounds were then tested as substrates for the...
- 8
- 537
- 0
Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... biosynthesis of the lipopeptides The experiments performed in the present study showed that this AAT can form Glu and Asp, and the formation of Glu and Asp is used to synthesize Gln and Asn, respectively ... stabilizes the protonated N(1) of the co-enzyme to strengthen the electron-withdrawing capacity of the co-enzyme [15] The active site Lys258 transfers a proton from the amino acid substrate to the cofactor ... PLP, whereas the Arg403 residue is responsible for recognizing the a-COO) of the substrate These functions are performed by the corresponding residues of Asp222, Lys258 and Arg386 of the AAT from...
- 13
- 490
- 0
Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in the presence of saturating levels of ... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another ... glucose (G) shock Disruption of this gene did impair development of hyphae and did decrease the infectivity of the organism, but it was not lethal Thus, the rate of growth of the mutant at 30 °C was...
- 10
- 428
- 0
Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the papers can be eluted to isolate the individual sugars which can then ... catalytic mechanism of TreS, each of the radioactive substrates was incubated with the enzyme in the presence of high concentrations of the unlabeled product, and the formation of radioactive product ... high-speed centrifugation and TreS activity was located both in the supernatant fraction and in the pellet However, the majority of the activity in the pellet could be released into the soluble fraction...
- 11
- 459
- 0
Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
... in the presence of glucose and tamXG, 38% of the added XG was incorporated into the cellulose pellicle and microscopic analysis showed the formation of cross-linkages between the ribbons [6] The ... same way except for the presence of the linker and the family-2 CBM at the C terminus of XEG74 The molecular masses of Cel9A-68 (CD + family3c CBM) and Cel48ACD, both cloned with the Cel6A signal ... known amounts of substrate (0–1500 lg), and the approximate relationship between the glucose standard curve and the nmoles of XGOs produced was determined for both the PAHBAH and the bicinchoninic...
- 9
- 453
- 0
Báo cáo khoa học: Gene cloning, expression and characterization of avian cathelicidin orthologs, Cc-CATHs, fromCoturnix coturnix pdf
... basis of the end of the 5¢-UTR and the first 20 bp of the fowlicidin signal peptide cDNA sequence, a set 1574 of primers was designed Several positive clones containing inserts of 545, 530 and 555 ... inhibiting the synthesis of one of their cell wall layers [40,41] Cathelicidins can kill both Grampositive and -negative bacteria, enveloped viruses including HIV, and fungi including Candida and Cryptococcus ... analyses of Cc-CATHs and avian cathelicidins on the basis of the proregion (A) and mature domain (B) The phylogenetic dendrogram was constructed by the Neighbor-joining method based on the proportion...
- 12
- 406
- 0
Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... NaoA The pI of NaoA is about 5.2 according to the standard plot between the protein’s pI and its migration distance (cm) in IEF The optimal pH and temperature of purified NaoA Fig Assay of NaoA ... reading and help in preparation of this paper REFERENCES Venulet, J & Van-Etten, R.L (1970) Biochemistry and pharmacology of the nitro and nitroso group In The Chemistry of the Nitro and Nitroso ... volume Nitrite released from the reaction was determined by the method of Little [28] One unit of NaoA is defined as the amount of enzyme required to catalyze the formation of lmol nitriteÆmin)1 Carbonyl...
- 6
- 255
- 0
Báo cáo y học: "Cloning, expression and characterization of gE protein of Duck plague virus" doc
... (underlined) and the first 25nt of the gE ORF, and the reverse primer (P2) 5'-CCGCTCGAGTCAGATGCGGAAACTAGA-3', with a XhoI site, protective base (underlined) and the last 18nt of the gE The PCR reagent ... experiments These results indicated that the antiserum had a high level of reactivity and specificity, and the antiserum was used for further experiments to study the intracellular localization of the ... mediate the intracellular traffic of DPV gE protein The research will provide useful clues for further understanding the localization properties of the alphaherpesvirus gE homologs Currently, there...
- 11
- 282
- 0
Cloning, expression and characterization of novel helicobacter pylori differentiating antigen heat shock protein 20
... become the prerequisite for the persistence of H pylori on the gastric epithelial cells and the subsequent immune responses of the host To begin with, adherence of H pylori on the gastric epithelium ... between the Dutch and Chinese isolates was the highest They therefore concluded that cagA-positive H pylori populations in China and the Netherlands are distinct Similarly, in the study of vacA ... Characteristics of Helicobacter pylori Two morphological forms of H pylori cells were observed: spiral form and coccoid form Spiral-shaped H pylori is the active form which is capable of colonization and...
- 301
- 525
- 1
Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae
... 4.5 Understanding of the nature of the interaction between Osh7p and Vps4p and future directions 108 4.6 Understanding the nature of Osh7p modification 109 4.7 Future directions of the project ... al., 1993) Thus, the regulation of sterol homeostasis appears to require many of the similar genes and proteins in yeast and human and much of the work in defining the role of sterol in eukaryotic ... of the membrane proteins within the limiting membrane of the endosome is sorted into these invaginating vesicles Subsequent fusion of the mature MVB with the vacuole results in the delivery of...
- 123
- 381
- 0
Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... PCR method To amplify the coding region of the x-5 gliadin gene, PCR primers were designed at the position of the initiation and termination codons of the gene based on the nucleotide sequences ... performed on serum from each of the three patients with WDEIA and who had been diagnosed by provocation test, to compare the IgEbinding ability of nOG5 and rOG5C The IgE antibodies in the sera of ... identical and the 1413 bp DNA of the sequenced x-5a gene is the same length as the PCR products indicating that the cloned x-5a gene has no artificial deletion The existence of repeat sequences of QQXP,...
- 8
- 484
- 0
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The ... isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence ... form the helices in the human IL-10 sequence show a degree of similarity to the carp IL-10 sequence The most highly conserved stretch in the IL-10 family is at the C-terminus in the C-helix and...
- 8
- 584
- 0
Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... understanding of their physiology In conclusion, we successfully performed cDNA cloning of EcR and USP of L decemlineata, and in vitro translation of corresponding receptor proteins The translated proteins ... to previously reported EcR A-isoform of other insects Thus we concluded that the longer cDNA encodes L decemlineata EcR A-isoform (LdEcR-A) On the other hand, the shorter sequence (488 amino ... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L...
- 15
- 564
- 0
Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... cycles) and at 72 °C, for a total of 21 cycles The PCR product was puri®ed using a QIAquickTM kit and cloned into the BamHI and XhoI sites of the pGEX-4T-1 expression vector The sequence of the insert ... lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lg of GST and 0.5 lg of ... sensitivity to the binding of GTP [8] There is support for this view from the crystal structure of the complex of Rab3A/GTP/Mg2+ with the effector domain of rabphilin-3A, which shows that elements of RabSF1,...
- 13
- 481
- 0
Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue specific expression and characterization of the lung isoform ppt
... other species, except in the case of sheep tryptases and [13], where the identity reaches 82–83% The major and more significant difference between BLT and BLCT resides at positions 188–189 of the ... Furthermore, analysis of BLCT and BLT expression in bovine heart and spleen has shown that both enzymes are present in these tissues at the mRNA level The simultaneous expression of the two isoforms ... electrophoresis and the bands eluted, purified and subcloned in the PCRIITM TOPO vector containing the lac promoter and the b-galactosidase gene, using the TA Cloning Kit (Invitrogen, USA) Transformation...
- 11
- 527
- 0
Báo cáo khoa học: Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish Molecular cloning, expression, characterization and ontogenic study ppt
... the synthesis of the first-strand cDNA using the First-Strand cDNA Synthesis Kit (Amersham Biosciences), according to manufacturer’s instructions One-microliter aliquots of the 33 lL first-strand ... amplify the complete coding region of this novel zebrafish SULT With zebrafish total RNA as the template and oligo(dT) as the primer, the first-strand cDNA was synthesized using the First-Strand cDNA ... detected in the embryos until the segmentation period (12–24 h post fertilization); it then gradually increased to a high level of expression throughout the rest of the embryonic development and the...
- 10
- 336
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu