construction of capsid modified adenoviruses by recombination in yeast and purification by iodixanol gradient
Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx
... bias in the selection of recombination points It has been suggested that optimal recombination points allow swapping of structural blocks [19], and combination of large protein fragments in our ... All together, recombination induced swapping of amino acids for 94 positions (19% of the residues) in the original LAC3 sequence The C- and N-termini of the hybrids were 33–37% and 9–26% different, ... recombination preserved the integrity of domain D1 and the very end part of domain D3, two regions that interact precisely in the natural laccase fold (Fig 5) Because of the bias introduced by linearization...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All of the ... mini-gel columns of Sephadex-G50 prepared in advance In brief, incubation mixtures of 200μL containing unloaded matrine and RGD-M-LCL were added onto mini-gel columns Then particles of RGD-M-LCL ... was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, USA) for 15 at room temperature in dark Binding buffer (400 μL) was then added to each tube, followed by cytometric...
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Multi wall carbon nanotubes (MWCNTs) doped polypyrrole DNA biosensor for label free detection of genetically modified organisms by QCM and EIS
... of this couple of electrodes is aimed at eliminating the change of charges in solution allowing the improvement of the signal to noise (S/N) ratio of WE Morphologies of pure PPy, PPy before and ... obtained by the PCR of ODN probe (P35S, left band) and its complementary target (right band) 1166 T.T.N Lien et al / Talanta 80 (2010) 1164–1169 Fig Schematic representation of working interdigitated ... that has been integrated into a crop consists of a promoter (P), a structural gene (‘encoding region’) and a transcription terminator (T) Two particular sequences are inserted in most of the available...
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Báo cáo y học: "Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line" ppsx
... hypothalamus; and in non-serotonergic neurons of the caudal hindbrain Materials and methods Mice Strain information is summarized in Table Pdx1-cre and Z/EG mouse lines were maintained as independent ... similar pattern of Cre-mediated recombination in the developing and adult hypothalamus and brainstem Furthermore, Wicksteed et al observed a similar pattern of recombination in an independently ... caudal hindbrain (ch) (F) and hypothalamus (hp) (H) In the rostral hindbrain, GFP expression occurred in the serotonergic dorsal raphe and caudal linear nuclei (B, D) In the caudal hindbrain, GFP...
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Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"
... persistence of IgE anti-Influenza virus antibodies in serum of IgE positive and negative vaccinated pediatric and adult subjects, approaching two years post vaccination The exact role of IgE in Influenza ... subject, no history of infection or vaccination Cytokines in serum Similar levels of IL-2, IFN-γ, IL-4, and IL-10 cytokines were detected in serum of vaccinated compared with non vaccinated subjects ... presence and persistence of IgG and IgE anti-influenza antibodies in the serum from those with normal and ele- 243 vated levels of IgE are understandable Our findings appear to be of importance and...
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A study of opening conversation on the phone in english and vietnamese
... requesting, inquiring and asking to start a telephone conversation advising, order, request…., Expressive structure with functions: complaining, apologising, complimenting and thanking,… Finally, ... Maxim’s and Brown & Levinson’s theory ,there are in ways of speaking He stated that the most important point to Cooperative Principles and Politeness Principles [11], [2] consider in studying and ... implications for the teaching and learning of conversational openings on telephone in terms of syntactic and English as a foreign language in Vietnam pragmatic features Opening and closing conversation...
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A study of idioms containing terms for plants in english and vietnamese
... meaning 4.4 SIMILARITIES AND DIFFERENCES IN ENGLISH AND of an English and Vietnamese idiom containing TsFPs because its VIETNAMESE IDIOMS CONTAINING TsFPs meaning can be made by associating its individual ... translation of English idioms containing TsFPs into Vietnamese: + Interpreting the denotative meaning of the given idiom containing TsFPs in the source language + Clarifying the connotative meaning of ... the basis of real-world In translation, understanding idioms in general and idioms situation, history allusion and cultural background containing TsFPs in particular as well as finding the translational...
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A contrastive analysis of encouraging as a speech act in english and vietnamese
... authors such as greeting, requesting, advising, offering, prohibiting, thanking, expressing, gratitude and etc However, little attention has been paid to the speech act of encouraging In this paper, ... feelings, offering help, predicting better future possibilities, suggesting things to do, giving advice, expressing belief 3.3.2 Encouraging and off- record strategy Giving encouragement There are kinds ... and lower- status FINDINGS AND DISCUSSIONS 4.1 OVERALL NUMBER OF STRATEGIES situations Both English and VNS used more appreciating someone, offering help and giving encouragement strategies in...
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Tài liệu Socio-demographic characteristics and sexual health related attitudes and practices of men having sex with men in central and southern Malawi doc
... Statistical Office guidelines (Muula, 2007b) These guidelines define rural, urban and peri-urban area status based on a decision by the Office of President and Cabinet and not based on development indicators ... spread of HIV in Malawi Materials and Methods Study area The study was conducted in the central and southern regions of Malawi and included Blantyre, Zomba, Lilongwe, Machinga, Mangochi and Dedza ... within prisons In some sense therefore this is an acknowledgement of the practice of MSM within prison environments The prevalence of MSM outside prison and jails in Malawi remains understudied and...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
... Comparison of the fasciclin I domains in CLAP with selected proteins in the protein database The Conserved Domain Database of NCBI was screened with the protein coding sequence of clone of CLAP and ... Processing of the pro-CLAP C-terminus is essential to lower the molecular mass and pI of the protein to values observed by SDS/ PAGE and IEF Interestingly, pool sequencing of the mixture of CNBr ... domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46] Fas I domains in proteins are almost always found in...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... also contains a GAL4 gene under the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the ... I7 OR in yeast (A) Luciferase bioluminescence was measured following yeast stimulation by odorants in two strains expressing either I7 OR and Golf, or I7 OR and Ga15 Strains were grown in minimal ... demonstrated that mRNA of the olfactory receptors is present in both uninduced and induced yeast cells (Fig 1) This indicates a leakage of GAL1 ⁄ 10 promoter in glucose-containing minimal medium The...
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Output of a Seminar on Energy Conservation in Paper and Pulp Industry pot
... Production process of the pulp and paper industry The pulp and paper industry has been consuming much energy and water ever since Cailun (an inventor of paper in ancient China) invented paper in A.D 105 ... of shives and impurities Trimming water cutting fault crushing Breaking by foreign substances Breaking in machine directions Faulty formation Incorrect roll crowing Mixing of shives and impurities ... formation and wrinkling Unevey drying and wrinkling - 14 - Promotion of screening Dryer cleaner Bronze Doctor, Dryer Grinding Centricleaner Flow rectify in Head Box &Wire Part Grinding Centricleaner,...
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Báo cáo khoa học: Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae potx
... SBA binding to HvALP as well as to other BBMV proteins, indicative of toxin binding to terminal GalNAc residues on these proteins Binding of SBA to the 170-kDa APN was almost unaffected by the ... nonreducing end of the oligosaccharide Absence of RCA-I binding to HvALP suggested lack of terminal galactose, confirming that SBA and WFL were binding to a terminal GalNAc residue Terminal GalNAc in ... and E coli infection in humans [49,50] Binding of Cry1Ac to proteins of 68-kDa in size in ligand blots of H virescens BBMV has been reported previously [1,5,11] Our ligand blotting and competition...
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THE RELEVANCE OF FORENSIC ACCOUNTING TO FINANCIAL CRIMES IN PRIVATE AND PUBLIC SECTORS OF THIRD WORLD ECONOMIES: A STUDY FROM NIGERIA potx
... examiners and interpreters of evidence and facts in legal cases that also offers expert opinions regarding their findings in court of law The science in question here is accounting science, meaning ... Reviewing of the relevant documentation to form an initial assessment of the case in an identified area of loss Examination for discovering, including the formulation of the act regarding the financial ... Organization of Forensic Accounting Profession Forensic Accounting is an offshoot of the general professional accounting and it is affiliated to professional accounting institutes For the purpose of practice,...
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Báo cáo hóa học: " Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients" docx
... confirming the results of former studies investigating Vβ-repertoire restrictions in blood of patients with other tumor entities, which have been interpreted as indication for the induction of specific ... measurements and analyses NCN collected samples and participated in the conduct of the study ET participated in design and conduct of the study UK participated in design and conduct of the study ... from average given in fold of standard deviation of the respective family (C) The sum of the modulus' normalized concentrations of all families of the samples (CD) and the number of families elevated...
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Báo cáo sinh học: " Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice" pot
... Ministry of Defence (Govt of India), Gwalior, MP-474 002, India e Defence Research and Development Establishment, Ministry of Defence (Govt of India), Gwalior, MP-474 002, India *Corresponding ... investigated the feasibility of using Pichia pastoris as an expression host for recombinant production of H1N1 HA protein and elicitation of neutralising antibodies against the same protein in ... concentrations of Geneticin also showed intensive bands in genomic DNA PCR and these results were in agreement with that of Geneticin sensitivity assay Optimisation of expression parameters and purification...
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báo cáo hóa học: " A review of health utilities across conditions common in paediatric and adult populations" pot
... our findings with, our review complements the recent review of generic and disease specific instruments in children and adolescents [5] by identifying studies reporting utilities in children and ... involved in the design of the study, interpretation of the data, and drafting the manuscript RBH participated in the data collection, analysis and interpretation of data LG was involved in the ... study and its design, analyzed and interpreted the data and wrote the manuscript NB participated in the data acquisition, data analysis, and the writing and editing of the manuscript MB was involved...
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Báo cáo hóa học: " A new electromechanical trainer for sensorimotor rehabilitation of paralysed fingers: A case series in chronic and acute stroke patients" pdf
... pain, touch, two-point discrimination, and position sense All patients were already participating in a comprehensive in- patient rehabilitation programme including 45 of physiotherapy and 30 of ... electromechanical Finger Trainer to move individual fingers in a physiological range of movement This article describes the device and reports its use in a small number of chronic and acute stroke ... Fischer et al assisted the finger extension of mildly affected stroke patients with the help of a powered orthosis Following six weeks of training in reach-to-grasp of virtual and actual objects, patients'...
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Báo cáo hóa học: " Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice" pot
... Ministry of Defence (Govt of India), Gwalior, MP-474 002, India e Defence Research and Development Establishment, Ministry of Defence (Govt of India), Gwalior, MP-474 002, India *Corresponding ... investigated the feasibility of using Pichia pastoris as an expression host for recombinant production of H1N1 HA protein and elicitation of neutralising antibodies against the same protein in ... concentrations of Geneticin also showed intensive bands in genomic DNA PCR and these results were in agreement with that of Geneticin sensitivity assay Optimisation of expression parameters and purification...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25