collection processing and statistical analysis
Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells
... outstanding research groups at the Department of Biological Sciences from National University of Singapore and Institute of Bioengineering and Nanotechnology for providing technical assistance and ... colloid For example, Harada and Kataoka used a small and bulky protein, the chicken egg white lysozyme (diameter nm), and Bronich used a surfactant micelle (Harada and Kataoka, 1998; Bronich et ... solutions containing anionic and cationic materials, such as complexes of polymers, metal and oxide nanoclusters, cage-structured molecules such as fullerenes, and proteins and 18 Introduction other...
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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... homogenizing, and were cooled down to 40 to 55°C In phase 4, they were treated for 10 to 120min., with addition of some amount of proteinase and amylase complex including α-amylase, β-amylase, and glucoamylase ... derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of the fluid of usually cocked rice itself ... strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively (data not shown) Effect on...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... collapsed at 49 mNặm)1 and 39 A2 At 37 C, the two-dimensional transition was absent and only a liquid-expanded state was evident For all derivatives, molecular dimensions increased and compression forces ... with tight lipid packing, and induced low reporter gene expression Table Midpoint and onset phase-transition temperatures obtained from curve ts and rst-derivative analysis, respectively, of ... chloroform and loaded onto a silica gel column (26 ã 2.8 cm) The column was eluted sequentially with 100 mL chloroform, and 1, 3, 5, 7, 8, 9, 10, 12 (300 mL) and 15% methanol chloroform The 10% and...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... band was visible The nested reaction (lanes 4–6) amplified a fragment of 166 and 121 bp at-3¢ and 5¢ end of the vector DNA, respectively M: 100 bp marker; lanes and 4: blank reaction; lanes and ... in Materials and methods and cells were collected at different time points from infection; lysates were assayed for protein content and enzyme activity (mean ± SD, n ¼ 3) (B) Alu-PCR analysis to ... amplification, differing in dNTPs and primer concentrations (0.2 mM and 0.5 lM, respectively) and in the annealing temperature, which was 55 °C for the 5¢ nested PCR and 58 °C for the 3¢ nested PCR...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... derivatizing the single standard polyamines (aliquots ranging between 0.125 and 0.5 lg for Put and Spd, and between 0.5 and lg for Spm) Each standard sample was run in triplicate, and the mean value ... existing chemical and thermodynamic equilibrium between reagents (polyamines and phosphates) and products (ivNAPs) [8] Furthermore, our data suggest that self-structuring of polyamines and phosphate ... mesostructural and microstructural scales, a specific study based on DLS and small-angle neutron scattering measurements would be required In any case, the analysis of both the correlation function and the...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... contributions YP performed the design and execution of the experimental setup and analysis, as well as drafted the manuscript CA executed and analyzed the experiment, performed statistical analsys as well ... translational and rotational migrations and micromotions (p < 0.01) There was, however, a strong interaction between the abductor and the Fap present in all the motion resultants (p < 0.01) and all ... migration and micromotion components for both groups at all loading conditions are summarized in Tables and The resultants of these components, i.e total translational and rotational migrations and...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... mass spectroscopy and elemental analysis The IR spectra of compounds (e) and (f) showed the absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, ... chemistry and antioxidant properties of 5H-dibenz[b,f]azepine and its derivatives Six molecules (a–f) were synthesised, and their structures were established by chemical and spectral analysis ... concentrations is shown in the Figure Compounds (a) and (d) showed 85.44% and 92.13% protection at µM level, 92.94% and 94.76% protection at 10 µM level, and 94.69% and 96.39% protection at 15 µM respectively,...
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Báo cáo khoa học: "Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes" docx
... Maclellan, L J., Lew is, I and Gardner, D K Current status of IVM/IVF and embryo culture in humans and farm animals Theriogenology 1994, 41, 57-66 Vajta, G., Machaty, Z., Barandi, Zs and Varga, Zs Embryos ... statuses and the feasibility of using oocytes from anestrus and pregnant cows to produce calves using IVF procedures Materials and Methods Bovine ovaries were immediately collected after slaughter and ... centrifuge tube and centrifuged at 100 g for 10 The pellet was resuspended with ml TALP and then allowed to stand at 22℃ for An additional ml TALP was added to the sperm suspension and centrifuged...
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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... mangium: growing and utilization, MPTS monograph series, No 3, Winrock International and FAO, Bangkok, Thailand, pp 1–19 [7] Kumar M.B., Barker R.E., Reed B.M., Morphological and molecular analysis of ... sterile sandy soil at the ratio of 1:1 (garden soil: sand) into earthen pots and kept under high humidity (70%) in a greenhouse with temperature (30 ± °C) for acclimatization RESULTS AND DISCUSSION ... growing on multiplication medium were excised and cultured on half-strength MS basal salts supplemented with 0, 0.25, 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... and AR50249 from to National Institute of Arthritis and Musculoskeletal and Skin Diseases to SCG and CHE, by grant STE 1051/2-1 from the Deutsche Forschungsgemeinschaft (DFG) to AFS and UN, and ... numerical data were subjected to variance analysis (one or two factor analysis of variance) and statistical significance was determined by student's t-test, and level of P < 0.05 was considered significant ... X and OP at day 3, IHH and ALP at day and RUNX2 at day 14 compared with Ad.GFP controls (Figure 7) These results suggest that BMP-2 and BMP-4 gene transfer induced a significant chondrogenic and...
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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf
... added and the plate was sealed and kept at 37°C in an atmosphere of 5% CO2 for 24 h After the herbal extracts treatment was over, removed the media and test compounds 100 μl fresh media and 20 ... presence and absence of SN and interferon for 24 48 hrs Cells were harvested and relative RNA determinations were carried out using semi-quantitative RT-PCR The results demonstrated that SN and interferon ... days of infection in 24-well plates in the presence and absence of herbal extracts and grown to 80% confluence with ml medium After 24 h, cells and total RNA was isolated by using Gentra RNA isolation...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
... the median-effect analysis and the MacSynergy II analysis led us to analyze the combination using three other methods: the isobologram analysis, the Berebaum combination indices analysis with weighted ... median-effect analysis, isobologram analysis and the Yonetani-Theorell Plot analysis, all of the results showed the TFV-DP+FTC-TP combination to be synergistic The discrepancy between the MacSynergy II and ... metabolites TFV-DP and FTC-TP [30]; and (2) DEC or DEC-like complex formation by TFV-terminated DNA and HIV-1 RT in the presence of the FTC-TP or EFV, and by FTC-MP-terminated DNA and HIV-1 RT in...
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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps
... interpretation and in silico analysis, and drafted the manuscript LG performed GST-pull down/ Western blotting experiments NOD participated in the experimental design and performed LC MS/MS and peptide and ... HDAC-1 AND -2 and RbAp46/48 and additionally contains CDH4/Mi-2, MTA1/2, MBD3, and MINT NuRD is recruited to target genes via DNAbinding proteins, such as Ikaros identified here by our approach and ... structure and RNA processing complexes We also noted the enrichment of three crucial motifs (HELIC, DEXDc and AAA) associated with helicase and ATPase activities essential for RNA processing, ...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... KS, Lareu R, Rajagopalan R, et al 2010;Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids-in silico predictions and in vitro evidence Nucleic Acids Res (1):172-181 ... 1998;Procollagen Nproteinase and procollagen C-proteinase Two unusual metalloproteinases that are essential for procollagen processing probably have important roles in development and cell signaling Matrix ... 2009;Tissue architecture and function: dynamic reciprocity via extra- and intra-cellular matrices Cancer Metastasis Rev (1-2):167-176 93 Maxwell CA, McCarthy J, Turley E 2008;Cell-surface and mitotic-spindle...
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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... particle synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript ... concentrations of A- and HA being 95% and 5% at pH and 15 and 85% at pH 4, respectively (pK a = 4.75) Furthermore, even though the concentration of the anion changes in this case as well, and is higher ... particle-containing solution onto a glass slide and then drying under vacuum Scanning electron microscopy and energy-dispersive Xray analysis SEM images and EDX spectra were obtained with a Quanta...
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báo cáo hóa học:" The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro" docx
... proliferation in vitro, and the real-time cell electronic sensing (RT-CES) assay also provided the dynamic process and binding behavior between cells and related agents Experimental Reagents and Materials ... in 96-well plate and incubated for 24 h and then added the relevant materials and incubated at 37°C with 5% CO2 for 72 h Then, 20 lL, mg/mL MTT solution was added into the well and continue cultured ... 10-7 mol/L); c 2.5 lg/mL of ZnO NPs and daunorubicin (1.0 10-7 mol/L) and d 5.0 lg/mL of ZnO NPs and daunorubicin (1.0 10-7 mol/L) The information of ZP6 and ZP7 was shown in the supporting information...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps...
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báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx
... mobility µ and Smoluchowski's equation, ζ = µη/ε, where η and ε are medium viscosity and dielectric constant, respectively All Dz and ζ were obtained at 25°C, h after mixing Optical spectra and aggregation ... Figure 10 between Candida albicans viability (%) and zetapotential (mV) Correlation for AmB formulation at low (ᮀ) and high P (l) Correlation between Candida albicans viability (%) and zeta-potential ... Squibb (Brazil) and was initially prepared as a g/L stock solution in DMSO/methanol 1:1 Candida albicans ATCC 90028 was purchased from American Type Culture Collection (ATCC) and reactivated...
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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... characteristics and in vitro performance is studied Moreover, in vivo pharmacokinetic study, complete blood count analysis and toxicity assessment through haematology assay and histological analysis ... interstitiumand cells Selective accumulation of nanocarriers and drug then occurs by the EPR effect (Figure and 7A) (Haley and Frenkel, 2008) The EPR effect is now becoming the gold standard in ... receptors and usually expressed more abundantly in malignant tissues than in normal tissues because of the higher iron demand for faster cell growth and division of the malignant cells (Vyas and Sihorkar...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25