coli expression strain with the mutated plasmid construct
STRUCTURE ANALYSIS OF POLYSACCHARIDE ESTERS
... random cleavage Within the scope of this chapter, the analysis of the distribution of ester functions within the RU is broadly discussed In the next chapter, synthetic paths for the achievement ... positions 2, and within the repeating unit The triplet of the acetyl and the triplet of the propionyl moieties are distinctly separated from each other Fig 8.9 13 C NMR spectra of the carbonyl region ... confirming the assignment Consequently, the spectral integrals of the protons of the AGU and the methyl protons of the propionyl moiety can be applied to calculate both the partial and the overall...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... intermolecular b sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity of the other monomer For the FeR dimer, the N-terminal ... was initially digested with EcoRI, blunt-ended with the Klenow fragment and then digested with HindIII to give the expression vector pMKFLR9-10 E coli was transformed with pMKFLR9-10, and transformants ... [5,6] The crystal structure of FeR has been determined with and without NADP+ [6] These authors were unsuccessful in their attempts to solve the structure in the Fe(III) ion-bound state The catalytic...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... interaction with the side chain of Lys10 The unusual chemical shift values of the side-chain protons of Lys10 are consistent with the vicinity of the aromatic ring Other residues giving NOE peaks to the ... Furthermore, the results of the H±D exchange experiment suggest a signi®cant difference in the internal ¯exibility of the molecules The rigid nature of the second b strand is consistent with the ... 5C), there are some small structural differences The most important is the different orientation of the binding loop with respect to the rest of the molecule, although both the 11±16 loop and the...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... truncated C-peptides lacking the five Table Measured and theoretical masses of the pancreatic islet peptides The experimental masses agree well with the theoretical masses of the candidate peptides ... and the detected fragments from the LC-nozzle-skimmer and LC-ECD FTICR MS experiments The length of the peptides and the number of detected fragments in the fragmentation experiment reflects the ... and were identified, while the other prominent peaks represent the B-chain of insulin (*) and the truncated form of the C-peptide (**) High mass accuracy is provided using the FTICR technique, and...
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báo cáo hóa học:" Growth and structure analysis of tungsten oxide nanorods using environmental TEM" doc
... correlates with the longer direction of the nanorod, and the other cyclic spot aligned with the B vector, which correlates with the direction perpendicular to the longer direction of the nanorod The ... of the nanorods; the steps grew and moved to the top of the nanorods, as observed from the [100] direction in Figure 5a The steps were not confirmed on the edge of the nanorod observed from the ... and are the closest and close-packed planes [16], grew The origin of the tungsten is the bottom of the tungsten wire, so this acts as the tungsten supply for the nanorods Therefore, the growth...
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Báo cáo y học: "Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation" pot
... probe sets The higher the signal intensity is on this reference chip, the more red the color; similarly, and the lower the signal intensity, the more green is the curve's color (following the spectrum ... recognition site in their promoter Because of their high identity to the NFI consensus sequence combined with phylogenetic First, NFI-A might alter the expression levels of other transcription ... targets within the group of dysregulated genes investigated The limitation to the kb region upstream of the transcription start excludes all potential NFI sites that might be located either further...
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Crystal structure analysis of pils, a type lvb pilin from salmonella typhi
... unit-cells with their edges forming a grid or lattice The line along the a direction is called the x-axis of the lattice; the y-axis is in the b direction and the z-axis is in the c direction The x-, ... In the Ewald construction, a sphere with diameter 1/λ is drawn, centered at the crystal The reciprocal lattice is then drawn on the same scale as the sphere with its origin located 1/λ from the ... depend on the position within the unit-cell, but only on the orientation Hence, we can calculate the Patterson for the model in different orientations, compare it with the Patterson of the data,...
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A study on the structure of the speech “ I have a dream” by Martin Luther King A systemic functional grammar analysis
... Aims of the study In carrying out the research, the writer aims to: Illustrate the key concepts in FG Analyze the structure and meaning of the speech “ I have a dream” by Martin Luther King ... are closely related to the study will be examined in detail 1.4 Methodology of the study The structure and meaning of the speech will be focused in the study, therefore the methodologies applied ... of the rationale, aims, scope, methodology and design of the study Chapter II: Theoretical Background, illustrates the theoretical background for the main concepts in FG Chapter III: The...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... % SDS) The stringency of the washing step (at 48°C) was adjusted by lowering the sodium chloride concentration to achieve the appropriate specificity The slides were then rinsed briefly with ddH2O ... NO2- in the oxic biofilm strata, that is, the active NH4+-oxidizing zone is located in the outer part of the oxic biofilm, whereas the active NO2 oxidizing zone is located just below the NH4+-oxidizing ... 1996) The autotrophic nitrifying biofilms were first cultured with the primary settling tank effluent from the Shoseigawa municipal wastewater treatment plant, Sapporo, Japan for a few days and then...
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An Analysis of Domestic Water Demand Structure and Water Recycling in Beijing
... because the frequency of water use was clearly different in these seasons The other questionnaire was answered by the household at the end of the six-day survey for each day of the study period The ... families living in the Haidian District of Beijing City The Haidian District is located in the southern area of the city, northwest of downtown Beijing (Fig 1) The Haidian District with a population ... works in the city We used the 2005 Statistical Yearbook as the basic data for this study As discussed later, we conducted a survey of water demand in Beijing City in 2006 Therefore, the 2005...
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an economic analysis of financial structure
... February 2005, the Canadian Securities Administrators proposed the Internal Control Instrument and the Certification Instrument • These mirror the requirements of the Sarbanes-Oxley Act in the United ... Separation of ownership and control of the firm – Managers pursue personal benefits and power rather than the profitability of the firm Tools to Solve the Principal-Agent Problem • Monitoring ... knowledge about the other party involved in a transaction Two types of asymmetric information Adverse selection occurs before the transaction Moral hazard arises after the transaction • Agency theory...
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02 three dimensional static and dynamic analysis of structure
... of the C matrix represents the strains caused by the application of a unit stress The temperature increase ∆T is in reference to the temperature at zero stress The a matrix indicates the strains ... that the stresses be expressed in terms of the strains and temperature change Therefore, an equation of the following form is required within the program: f = Ed + f (1.6) in which E = C-1 Therefore, ... Therefore, the zero -strain thermal stresses are defined by: f = - ∆T Ea (1.7) The numerical inversion of the x C matrix for complex anisotropic materials is performed within the computer program Therefore,...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... Subtraction of the m ⁄ z values for the fragments within a given ion series that differ by a single amino acid affords the mass and thus the identity of the extra residue in the larger of the two fragments ... spectrometer, and then allowed to react with multiply protonated peptides and proteins in the gas phase In the above ion–ion reaction, the fluoranthene radical anion transfers an electron to the [M + 3H]+3 ... for 42 of the proteins disagreed The observed differences could be assigned to: deletion of the N-terminal Met, truncations at either the N-terminus or C-terminus of the protein, and the presence...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... faster when the temperature is raised The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to retard the rise to Fm With dPQ, the J step ... constant of the JI phase compared to the amplitudes and rate constants of the two other phases The above should be governed by the balance between the rate of reduction and oxidation of the acceptor ... is due to the removal of nonphotochemical quenching (NPQ) caused by reduction of the PQ molecule bound in the QB pocket [6,7,13] The above ndings are in agreement with the most recent theoretical...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... lacks the large P6 extension and the last three nucleotides (AU XG) of the intron The sizes of the intron (InDGb) and 5Â exon (5E) are indicated The arrow indicates cleavage of the pre-RNA at the ... because of the relative lack of protection of the catalytic domain and the junction nucleotides that link it to the other major domain We attempted to probe the InDGb RNA with Fe2+-EDTA in the presence ... shades; a color bar is given on the bottom right The analysis under these conditions was repeated twice with similar results The accuracy of the protection data at the nucleotide level is for residues...
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Tài liệu Báo cáo khoa học: "Identifying Linguistic Structure in a Quantitative Analysis of Dialect Pronunciation" docx
... the greatest similarity with the dialects found in the Southeastern part around Malko Tyrnovo On the map in Figure it is also possible to distinguish the area around Golica and Kozichino on the ... [e].6 For example, the indices for the sites Aldomirovci and Borisovo with respect to the sound correspondence [e]-[i] were calculated in the following way In the file with the sound correspondences ... display of the data revealing relationships between the instances in the data set (Davison, 1992) On both the maps the biggest division is between East and West The border between these two areas...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... prepared with the reverse primer DML250R and the forward primer T7 (5¢-taatacgactcactataggg-3¢), which anneals to the T7 promoter located in the pGEM11Zf+ plasmid Amplification was carried under the ... directionally cloned in pGEM11Zf+ plasmid (Promega) at EcoRI/ NotI sites [33] Escherichia coli DH5a cells were transformed with the cDNA library plasmids and then plated on a 2YT agarose plate ... using the Big Dye Terminator Cycle Ready Reaction Kit with Amplitaq DNA polymerase according to the manufacturer’s instructions Amplification of the DM64 cDNA by PCR with specific primers The D...
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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... tissues with the anti-b2IV serum, whereas tissues from a littermate control were well stained This demonstrates that the antiserum reacts with laminin b2 but with no other laminin chain and no other ... 5), with inhibition curves similar in slope to that of the reference inhibitors b1IV and b2IV, respectively Together the data showed that these assays were highly speci®c and suitable for the ... were found As expected, the highest amounts of b1 chain were found in the EHS tumor, while the content of b2 chain did not exceed 0.2% The amounts of c1 chain were within the usual range of 436...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... integrals The trNOE data suggests that there is a different flexibility in the N-terminal and the C-terminal parts of the molecule due to interactions with the protein This is in full agreement with the ... to binding by analyzing the surface of the residues covered by the protein They also see an interaction of the C-terminal part RPAP with the surface of the antibody In the shorter peptide that ... Most of these contacts are with the binding cavity in the Fv domain However, there are significant additional contacts to the bottom of the next Fab (cf Figure 10) The surface covered by the nonbinding...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... analysis of the S aureus EF-G structure together with the recent T thermophilus EFG–70S complex structure with FA [22], we can now classify the mutations into four groups, A–D (Table 2) These perturb ... bond with Asp87 in the switch II region In the ribosome complex, the backbone carbonyl interacts with A2660 of 23S RNA [22] Domain V, in helix BV at the surface close to the interaction with ... interactions The N-terminal part of the strand interacts with the N-terminal part of helix CG in domain G, and the C-terminal part interacts with the turn before helix BIII in domain III The G664A,...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25