coli electroporation and production of library phage
Tài liệu Báo cáo khoa học: "Accumulation of Lexical Sets: Acquisition of Dictionary Resources and Production of New Lexical Sets" pdf
... multiplevalue function model of CLOS) Finally, the values of the output variables of the left-hand side nonterminal (aol, ao2 ) are collected and returned as the result of its expanding With some predefined ... establish and implement a formalism for specifying and doing production 2.1 Categories o f production Production can be done in one of two directions, or by combining both: "extraction" and "synthesis" ... information types and the number of set components in its entry structure For example, an English-French dictionary whose entries consist of an English headword, a part -of- speech, and a set of French...
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Báo cáo khoa học nông nghiệp " Effects of Stocking Biomass on Growth, Survival and Production of the Two Sizes of Clam Meretrix lyrata Cultured in the Intertidal Areas And Notes on Hatchery Production of Clam Spat " pot
... research to establish a standard protocol to enhance the production and profit of clam culture Among the factors that affect growth and production, feed and feeding of clam have been regarded ... The objective of this research was to evaluate the effect of stocking biomass of the two sizes of M lyrata on growth performance and survival to enhance the production and profit of cultivation ... species on production (Cigarrıa and Fernandez, 2000; Shpigel and Spencer, 1996; Zhang and Yan, 2006) and the use of clam as water quality improvement (Jara-Jara et al., 1997; Shpigel and Fridman,...
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Báo cáo nghiên cứu khoa học " CLAM CULTURE DEVELOPMENT IN THE INTERTIDAL AREA: EFFECTS OF STOCKING BIOMASS ON GROWTH, SURVIVAL AND PRODUCTION OF THE TWO SIZES CLAM Meretrix lyrata " docx
... clam aquaculture protocol to enhance the production and profit of clam culture Among the factors that affect growth and production, feed and feeding of clam have been regarded as the most important ... uncontrollable and variable, stocking biomass becomes an important factor to increase growth and production The objective of this research was to evaluate the effect of stocking biomass of the two sizes of ... and Wf are mean of initial weight and final weight, respectively and t is number of experiment days Size variation of the clam was evaluated according to Wang et al (1998) in which the mean of...
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Báo cáo lâm nghiệp: "partitioning and production of 15 N-labelled litter in beech trees" pps
... inner and lower crowns were randomly taken from different trees at regular intervals (weekly and fortnightly in 1993; monthly in spring 1994 and 1995) and before and after the application of [ ... and release of urea beech organs, and ii) that fallen leaves (i.e N litter) exhibited homogeneous 15 N from litter The determination of the sizes of the N pools involved and estimates of their turnover ... concentration and 15 analN yses of litter, tree and soil samples, about 6-10 mg of each sample were weighed in silver cups and carefully closed Samples were then combusted, N reduced to N and the 14...
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Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps
... are shown in Figure Reactions with isolate V1 and T 551 gave similar bands but some of the T 551 bands were weaker The patterns of isolates T 603 and T 657 were also very similar Discussion To ... out most of the laboratory analyses, the interpretation of the results and the manuscript preparation LLF carried out the RAPD analysis and contributed to the evaluation of the results HH and MP ... brennaborense and two thirds of the isolates belong to the T phagedenis-like phylotype However, phylotypes that have not been cultured have been identified through FISH and cloning of 16S rRNA...
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Beneficial uses and production of isotopes
... update of the publication on Beneficial Uses and Production of Isotopes issued in 1998 by the OECD It includes statistical data and analyses of key issues in the field of isotopes demand and supply ... demand and supply of isotopes and by providing analyses, including projections, on supply and demand issues In the light of the continuing changes in isotope uses and geographic distribution of ... evolution of techniques and the emergence of new procedures requiring the production of new isotopes Globally, the number of medical procedures involving the use of isotopes is growing and they...
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báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot
... diagnosis and prognosis of RCC Phage display is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage Phage display ... ODM13 and OD C1 represent the OD values from the selected phages and control phages binding to A498 cells, respectively OD S2 and ODC2 represent the OD values from the selected phage and control phage ... constructed at the N terminus of the minor coat protein (cpIII) of the M13 phage The titer of the library is 2.3×1013 pfu (plaque-forming units) The library contains a mixture of 3.1×109 individual clones,...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... analysis of chimeric RTB/p24 and p24 in E coli Expression analysis of chimeric RTB/p24 and p24 in E coli Total proteins were extracted from E coli cultures, separated by 10% SDS-PAGE and stained ... chromatography purification E coli expression of RTB/p24 and p24 HIV proteins Six E coli strains were tested to obtain the best levels of expression and solubility of the RTB/p24 and p24 proteins in cytosolic...
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Báo cáo khoa học: " The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1" doc
... resistance of bacterial cultures to bacteriophage wV8 Bacteria E coli O157:H7 Other E coli Sensitive to bacteriophage wV8 Resistant to bacteriophage wV8 Reference strains of 12 common E coli O157:H7 phage ... BLASTN of the genome of phages wV8 (top) and Felix O1 (bottom) Alignment, based upon BLASTN of the genome of phages wV8 (top) and Felix O1 (bottom) The contiguous black boxes under the phage names ... of wV8 as one of a cocktail of phages for control of E coli O157:H7 in cattle RPJ also contributed to manuscript preparation Additional file ClustalW alignment of the tail fibre proteins of phages...
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PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION
... MATERIALS AND METHODS Bacterial Strains One strain of enterohemorrhagic Escherichia coli O26 was provided by Prof M Fukuyama, College of Environmental Health, Azabu University One strain of vancomycin ... disinfection of treated wastewater in a large-scale pilot plant and inactivation of selected bacteria in a laboratory UV device Biores Tech., 74, 141–150 Kamiko N and Ohgaki S (1989) RNA coliphage as ... Lindenauer K G and Darby J (1994) Ultraviolet disinfection of wastewater: effect of dose on subsequent photoreactivation Wat Res., 28, 805–817 Schoenen D and Kolch A (1992) Photoreactivation of E coli depending...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 1 pptx
... Harvesting/concentration and cold storage of rotifers 3.7 Production and use of resting eggs 3.8 Literature of interest 3.9 Worksheets Worksheet 3.1 Preparation of an indicator solution for determination of residual ... Standard enrichment for Great Salt Lake Artemia 4.4 Tank production and use of ongrown Artemia 4.4.1 Nutritional properties of ongrown Artemia 4.4.2 Tank production 4.4.2.1 Advantages of tank production ... Literature of interest CLADOCERANS, NEMATODES AND TROCHOPHORA LARVAE 6.1 Daphnia and Moina 6.1.1 Biology and life cycle of Daphnia 6.1.2 Nutritional value of Daphnia 6.1.3 Feeding and nutrition of Daphnia...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 2 doc
... These various aspects of the production, use and substitution of micro-algae in aquaculture will be treated within the limits of this chapter 2.2 Major classes and genera of cultured algal species ... reproducibility, which is a function of the sampling, diluting, and filling of the counting chamber, as well as the choice of the right type of counting chamber and range of cell concentration Counting ... the growth of diatoms which utilize this compound for production of an external shell Micronutrients consist of various trace metals and the vitamins thiamin (B1), cyanocobalamin (B12) and sometimes...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 3 ppt
... 3.7 Production and use of resting eggs For the mass rearing of rotifers as larval food the amictic way of reproduction (see 3.3.) should be favored However, when the interest is in production of ... growth of the animal is assured by plasma increase and not by cell division The epidermis contains a densely packed layer of keratin-like proteins and is called the lorica The shape of the lorica and ... the profile of the spines and ornaments allow the determination of the different species and morphotypes (see 3.4.) The rotifers body is differentiated inTO three distinct parts consisting of the...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 4 pptx
... outputs, not only in terms of survival, growth and success of metamorphosis of many species of fish and crustaceans, but also with regard to their quality, e.g reduced incidence of malformations, improved ... resulted in optimized and cost-effective applications of this live food in hatchery production 4.1.2 Biology and ecology of Artemia 4.1.2.1 Morphology and life cycle 4.1.2.2 Ecology and natural distribution ... screening of candidate strains and of eventual local populations, as well as the study of prevailing environmental conditions Uncontrolled introduction of Artemia may thus lead to a decrease of natural...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 5 docx
... content and hatchability Hatchability of cysts is largely determined by the conditions and techniques applied for harvesting, cleaning, drying and storing of the cyst material The impact of most of ... function of the tank size and the density of cysts incubated Excessive foaming can be reduced by disinfection of the cysts prior to hatching incubation and/ or by the addition of a few drops of a ... The use of decapsulated cysts in larval rearing presents some distinct advantages, both from a practical and nutritional point of view The daily production of nauplii is labour intensive and requires...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 6 docx
... fast harvesting of large volumes of Artemia nauplii and allows complete removal of debris from the hatching medium This technique results in a significant reduction of labour and production costs ... Ph and Lavens, P 1995 Review of some aspects of marine fish larviculture ICES mar Sci Symp., 201:138-142 Sorgeloos, P and Léger, Ph 1992 Improved larviculture outputs of marine fish, shrimp and ... harvest and start enrichment 18:00 add second enrichment · Day - 9:30 harvest 4.4 Tank production and use of ongrown Artemia 4.4.1 Nutritional properties of ongrown Artemia 4.4.2 Tank production...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 7 pptx
... Enrichment of ongrown Artemia 4.4.2.8 Control of infections 4.4.2.9 Harvesting and processing techniques 4.4.2.10 Production figures and production costs 4.4.2.1 Advantages of tank production and tank ... behavior of Artemia by affecting the filtration rate, ingestion rate and/ or assimilation: including the quality and quantity of the food offered, the developmental stage of the larvae, and the ... leaching of body fluids (See also 4.5.7.1) 4.4.2.10 Production figures and production costs Figure 4.4.14 provides a summary of average production data expressed as Artemia survival and length,...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 8 pptx
... are usually low and mainly fluctuate in function of food availability, temperature and salinity The size and/ or often complete absence of suitable infrastructure makes management of such lakes ... very unprecise and inaccurate Of course sample volume depends both on animal abundance and animal size As both cyst production and biomass production mainly depend on the number of large animals, ... usually depends on the availability of high saline water and is often limited to certain periods of the year Management of these ponds is similar to the management of the Artemia ponds in artisanal...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 9 pptx
... Literature of interest 6.1 Daphnia and Moina 6.1.1 Biology and life cycle of Daphnia 6.1.2 Nutritional value of Daphnia 6.1.3 Feeding and nutrition of Daphnia 6.1.4 Mass culture of Daphnia 6.1.5 Production ... intensity, ) and biota (phytoplankton and copepod production, number of predators, bacterial turn-over, regeneration of nutrients from copepods and fish larvae) in such a way that the production of one ... nauplius and copepodite stages of copepods; · 300 and 500 µm for small water fleas and smaller species of cyclopoid copepods; · 700 µm for adult water fleas of the Daphnia genus, large species of cyclopoid...
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