... spruce stands in the Krkonoše Mts in years 1980, 1993, 1998 and 2003 gave unsteady values In this period, pH markedly increased (from 3.59 in 1980 to 4.77 in 1998) and in the 2004–2006 investigation ... žka 2006 Fig Index of active mycorrhizae (AM) density in 1994 and 2004–2006 in Růžová hora the average decrease was 0.70 g, in Sněžka 0.81 g (Fig 5) Changesin the parameters within the years ... parameters was observed In the years 2004–2006 the values of pH in Růžová hora varied from 3.65 3.97, those in Sněžka were similar 3.49–3.77 A high negative influence of the increase in acidity (even small...
... specificity of a recombinant cysteine proteinase, CPB, of Leishmania mexicana compared with cruzain, human cathepsin L and papain using substrates containing non-natural basic amino acids Eur J Biochem ... containing mM dithiothreitol Gelatin hydrolysis was detected by staining with Coomassie Blue R-250 (0.25% w/v) When analysing activities towards N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin (Suc-LY-MCA; ... directly, or they had an indirect effect byin uencing the enzyme’s folding and stability To investigate the role of the different amino acid residues we generated two mutants of CPB3 in which residues...
... Pregnancy-specific protein B in serum of postpartum beef cows J Anim Sci 1993, 71:2199-2205 Lindell J-O, Kindahl H, Edqvist L-E: Prostaglandin induced early abortions in the bovine Clinical outcome and ... commencing within 24 hours of inoculation/injection [5] or embryonic/foetal mortality [18] following experimental Arcanobacterium pyogens infection or cloprostenol injection On the other hand, in ... progesterone; bPAG1 = bovine pregnancy associated glycoprotein 1; PG = the main metabolite of prostaglandin F2α tration could be possibly explained by the effect of uterine contraction caused by the pulsatile...
... concentrations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), bile acids, glutamate dehydrogenase (GLDH), creatine kinase (CK) and creatinine were determined using an auto analyzer ... of swelling still remained in nine out of 35 dogs examined At the fourth examination (day 9-23) there was no residual swelling in the region of the snake bite in any of the dogs (Fig 1) In other ... creatinine values at examination and However, creatinine decreased from examination to examination in 82% of the sampled dogs (p < 0,05 Sign test) Discussion At the first examination within a...
... limiting conditions when chatting on Internet, such as spatial, temporal and social constraints The study “Linguistic Innovations and Interactional Features of Casual Online Communication in Japanese” ... basic linguistic features so as to help (Vietnamese) American English learners, especially those who are increasingly online for writing, posting and viewing comments 2 Thirdly, the increased ... top 10 AI perform in season 10 and we mainly focus on the findings of linguistic features; i.e grammatical features, pragmatic features, commonly-used expressions and paralinguistic cues of AWAI...
... Homocysteine metabolism 2.1 Plasma homocysteine As mentioned in the introduction homocysteine is a sulfur–containing intermediate in the normal metabolism of the essential amino acid methionine, ... either by genetic defects in the enzymes involved in homocysteine metabolism or by nutritional deficiencies in vitamin cofactors Homocysteinuria and severe hyperhomocystenemia are caused by rare inborn ... mutation in the coding region for the 5,10-MTHFR binding site (C677T), leading to the substitution of an alanine to a valine effectively increases homocysteine levels increase and decreases methionine...
... were recorded in a structured format, after clinical examination Stool, urine and blood samples (3-4 ml) were collected from subjects for investigation Routine blood examinations including complete ... referring cases to the hospital Contributors: BRK: Enrollment of cases in hospital, clinical examination and recording, data interpretation and manuscript preparation; BD: Field investigation, Clinical ... were observed in 11 cases The lesions were associated with itching in 24 cases which was more pronounced during the healing phase In the 46 cases having complete follow-up, average healing time was...
... protein and fat leads to an increase in free amino acids and short chain fatty acids (SCFAs) Organic acids such as acetate and lactate produced during fermentation by LAB lower the pH of intestinal ... given in the multi-strain treatment, resulting in larger numbers of probiotic bacteria competing with pathogens for binding sites and or nutrients in the gut Another point of interest in this ... shown promising potential in preventing both nosocomial spread of gastroenteritis and diarrhoea in infants in residential care settings Meta-analyses of double-blind, placebo-controlled clinical trials...
... cysteines present in other condensing enzymes To get more insight into how the coding region of the FAE1 condensing enzymes in Brassicas determines the proportions and amounts of VLCFAs in their ... examine the role of the amino acid serine at position 282 in the FAE1 protein sequence to determine if this apparent mutation from serine to phenylalanine led to the LEA B napus phenotype We introduced ... oleracea MDE line 103, B rapa MDE line R500 and LEA B napus cv Westar were aligned Amino acid residues at position 282 are shaded in black and indicated by the black arrow The amino acid residues...
... strong nature of the binding of ascorbicacid to proteins Although ascorbicacid is a small molecule, it blocks the most attractive binding subsites in the protein, leading to the inhibition of the ... prepared by soaking the crystals of native protein in the reservoir solutions containing ascorbicacid and lactose at a concentration of 100 mgÆmL)1 for 48 h Detection of ascorbicacidin crystals Ascorbic ... bonded interactions between HylP2 and ascorbicacid at three binding regions in the polysaccharide binding groove Table Hydrogen bonded interactions between HylP2 and lactose at three binding regions...
... These data confirm results obtained by others indicating that oxygen radicals play an important role in mediating quartz-induced COX-2 expression in macrophages, and indicate that their production ... response by macrophages, also stimulated by cytokines released by other cell types during the ongoing in ammation Results obtained here also demonstrate the specificity of action of AA on crystalline ... the principle of protein-dye binding Anal Biochem 72, 248–254 34 Singh IS, He JR, Calderwood S & Hasday JD (2002) A high affinity HSF-1 binding site in the 5¢-untranslated region of the murine tumor...
... acid) solution] Broken line, 30 lM T4 DNA in the absence of ascorbicacid We now consider the stability of the intrachain segregated structure induced byascorbicacidIn general, the free energy ... DNA in the presence of different concentrations of ascorbicacid (B) 100 lM ascorbicacid (C) Solid line, difference spectrum [(30 lM T4 DNA + 100 lM ascorbic acid) solution ) (100 lM ascorbic acid) ... images of individual T4 DNA molecules in aqueous solution at pH 7.4 in the absence and presence of ascorbicacid Depending on the concentration of ascorbic acid, DNA molecules exhibit intrachain and...
... serious infections Inducible resistance to clindamycin may be present in staphylococci (ie, they may be D-test positive); for these strains, clindamycin should not be used in high-inoculum infections ... regarding the efficacy of inhaled ribavirin for treatment of RSV CAP in infants Ribavirin has in vitro activity against RSV, but use of this drug for RSV infection is not routinely recommended in ... signs Clinically defined ‘‘toxicity’’ based on clinical judgment or change in mental status Chest pain, splinting of the chest Inability to maintain oral intake and hydration Extent of abnormal or...
... amino acids are assimilated in the form of peptides that are produced from proteins by LAB extracellular proteinase [8] The assimilated peptides are further hydrolyzed by peptidases in LAB in ... determined using the ninhydrin method [24] Dipeptides were hydrolyzed in 20 mm sodium citrate buffer (pH 6.5) ⁄ mm zinc chloride The reaction was initiated by the addition of enzyme solution At intervals, ... (C-terminus) restriction enzyme sites Recombinant Lactococcus lactis prolidase (indicated by underlining) that flanked the ends of the open reading frame The PCR reaction mixture contained genomic...
... of 1062 coding nucleotides, and only of them resulting in amino acid substitutions (Arginine, Glycine, Leucine and Glycine in A transferase and Glycine, Serine, Methionine and Alanine for B transferase ... contained a single nucleotide deletion at the 261 position, relatively close to the N-terminal of the coding sequence, resulting in a codon frameshift starting from amino acid 88 in the protein ... molecule binds to one of the b-chains of Hb favouring deoxygenation, reducing hemoglobin oxygen affinity Thereby, low 2,3-DPG concentrations increases the Hb affinity to O2, dislocating the oxyhemoglobin...
... genes involved in angiogenesis by staining and realtime PCR Cancer genesis in each group was identified by H&E staining as shown in Figure 4A We observed blue staining of giant nuclei followed by ... recovered in NIH3T3 cell at 12, 24, 36 hrs by cell migration even inascorbicacidin 2.5 mM and ascorbicacidin 10 mM (Figure 6) Therefore, migration was inhibited according to ascorbicacid concentration ... suggest that ascorbicacid treatment in high concentration inhibits angiogenesis by inhibiting the expression of angiogenesis related genes Inhibition of Cancer byAscorbicAcidin H-ras NIH-3T3...
... reflected in the alterations of light scatter Therefore, treatment induced changesin forward and side scatter of incident light were investigated by collecting these signals in the list mode using ... Uptake kinetics of AlPcS2 following incubation of cells with AlPcS2 in HBSS for different time intervals showed rapid and linear increase in the accumulation of AlPcS2 in the first h, prolonged incubation ... were incubated in growth medium, harvested and counted (using hemocytometer) after varying intervals of time Floating cells were collected separately before harvesting attached cells by trypsinization...
... eclampsia and clinical signs of uterine rupture had the highest rates at around % Total (100.0%) 2131 (100.0%) Table 3: Institution where the clinical officers did their internship against the Institution ... the performing surgeons, by noting information about the institution at which they did their internship as well as the number of years of practice each of them had after a completed internship ... within the next eight years Success stories from Sri Lanka [7] and Malaysia [8] point to human resources as a crucial factor in reducing maternal mortality In order to cope with the ever-increasing...