... bovine serum (GIBCO) and a mixture of penicillin-streptomycinglutamine (PSG from GIBCO) Puromycin-resistant MEF lines were grown, maintained, serum shocked, and serum starved in medium containing ... itself, and clinical features of depression such as premature early morning awakening from sleepand diurnal variation in mood, shortened REM sleep latency, advances in hormonal and temperature rhythms, ... differences in the molecular and genetic details of the circadian machinery in mammals and Drosophila, the basic regulatory principles are maintained [20,21] Central to the molecular circadian...
... of sleep span spent actually sleeping (% sleep) , sleep latency, sleep efficiency, frequency of long sleep episodes (long sleep episodes) Site Differences in Actigraphy Each patient’s baseline sleep/ activity ... for a linear increasing or decreasing trend and subsequently determining the probability that the slope of the best-fitting line is different from zero Using the same technique, the cosinor method ... the quality of circadianrhythmsand how well activity andsleep are each consolidated within the day Grutsch et al Journal of CircadianRhythms 2011, 9:4 http://www.jcircadianrhythms.com/content/9/1/4...
... proteins are indicated in grey and mouse proteins in orange; proteins indicated in black are found in both The regulators are bZIP type factors in fly and nuclear hormone receptor (NHR) proteins in ... to the DNA-binding domains of LUX, CCA1, and LHY One ELF4 homolog and several PRR-like genes containing a responseregulator domain and a CCT domain were also found ([27] and G.B and S.A.K., unpublished ... mouse In addition, the proteins containing the bHLH and PAS domains also regulate the expression of binding partners that will repress their action The PER protein acts with TIM in the fly to inhibit...
... its N-terminal region, but in contrast to CLOCK, both the PAS-A and PAS-B domains of NPAS2 contain a heme-binding site, and CO binding to the heme inhibits the DNA-binding activity of the NAPS2–BMAL1 ... neuronal PAS domain protein (NPAS2), which is homologous to CLOCK [7–10] NPAS2 plays an important role in maintaining circadian behaviors in normal light–dark and feeding conditions, and it is critical ... direct or indirect manner Therefore, it is worth studying how the bHLH domain contributes to heme binding to the PAS-A domain in NPAS2 and how the bound heme participates in the binding of NPAS-2...
... self-reported sleepiness before and after sleep We labeled those with more sleepiness in the morning than on the night before as 'a.m sleepier' and those with less sleepiness in the morning than on ... disturbing the sleep of this group of CFS patients is an imbalance of the cytokine sleep network (that is, sleep- producing and sleep- disrupting cytokines) in favor of sleep- disrupting cytokines ... of sleep than healthy controls In comparison with controls, sleepin CFS had little effect on either self-reported sleepiness or fatigue And, interestingly, for patients only, ratings of sleepiness...
... sleep period spent sleeping) are highest when sleep begins in the late evening and lowest when sleep begins in the middle of the day In the current study, both sleep duration and subjective sleep ... sleep quality was lowest for sleeps initiated during the daytime, and peaked for sleeps initiated at 00:00– 02:00 h These results indicate that the sleep of locomotive engineers working and sleeping ... fall in mean sleep duration from the daily peak for sleep initiated at 20:00–22:00 h to the daily minimum for sleep initiated at 12:00–14:00 h, and a sharp increase insleep duration for sleep initiated...
... Immunohistochemical stain for determining mPER1 protein expression in brains and kidneys a: Positive staining in the nucleus and cytoplasm are found in brains of Con, MD and MW mice b: Representative ... that circadianrhythms of clock genes including mPer1 were maintained in the kidneys of SCN-lesioned mice [28] In feeding studies, it was found that feeding schedules could entrain the circadian ... purposes) Journal of CircadianRhythms 2006, 4:9 http://www.jcircadianrhythms.com/content/4/1/9 Immunohistochemical stain for determining mPER1 protein expression in brains and kidneys Figure Immunohistochemical...
... Journal of CircadianRhythms 2006, 4:10 Background Measurement of locomotor activity is a valuable tool for analysing factors influencing behaviour and for investigating brain function As ... potential drift of signal detection in time, leading to false increase or decrease in movement In experiment 3, we were interested in the sensitivity of our system andin the comparison of our data ... video recording during darkness by using a camera that, by automatically switching to infrared in complete darkness, allowed us to continuously monitor activity during the light and dark phases...
... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell proliferation Also, increase in small-intestinal mucosa ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ), ... found to display prominent circadian oscillations in mouse brain reaching at its maximum in the afternoon/early evening hours and minimum in the night/early morning hours The circadian oscillation...
... of virus infections inhoneybees Although significant progress has been made inhoney bee virus research in the last few decades (Reviewed in Chen and Siede, 2007 [7], investigation into virus ... sequenced and analyzed [8-14] Using RT-PCR based assays, the virus infections inhoneybees can be detected and quantified in a rapid and accurate manner [15,16] As with all single-strand positive ... replication and tissue tropism of Deformed Wing Virus (DWV), a highly prevalent honey bee virus that causes wing deformity and mortality inhoneybees worldwide, in both bees with wing deformity...
... c) Pointers and values a) Value types and reference types in simple terms is nothing but conversion of a value type into a reference type a) Casting c) Unboxing d) Overriding b) Boxing ... for statement’s syntax is incorrect using System; class Test { static void Main() { int @Main; int[] Static= new int[3]; @Main =100*Static[1]; Console.WriteLine(@Main); } } What will be the output ... A.F Init B B.F b) Init A Init B A.F B.F c) d) A.F Init B Init A A.F A.F B.F Init B Init A 89 //.Inconsistent accessibility: base class 'Test' is less //accessible than class 'Q3' lỗi dòng using...
... of the following is the correct syntax for declaring an indexer a) protected int this[int var1] c) public int this(int var1) b) public int classname[int index] d public int this[int ) var1] A ... Main() { IndexerTest IndexMe = new IndexerTest(); IndexMe[1] = 100; IndexMe[2] = 1000; System.Console.WriteLine(IndexMe[1]); System.Console.WriteLine(IndexMe[2]); System.Console.WriteLine(IndexMe[3]); ... public string this[int pos] { get { return myData[pos]; } set { myData[pos] = value; } } static void Main(string[] args) { IntIndexer myInd = new IntIndexer(5); myInd[1] = "Some Value"; myInd[4]...
... workstations and mainframes within the Department of Computer Science, and supports projects in distributed computing and parallel processing such as the Camelot distributed transaction processing system ... be described in more detail in a later section When shadowing, the data is instead copied from the original 9 char *file_data; int i, file_size; extern float rand(); /* random in [0,1) */ /* ... a memory object and offset within that memory object Additional information stored for each range includes protection and inheritance information To account for sharing through inheritance, Mach...
... procedure's linkage section is made as a segment within that process In certain cases the linkage sections of several procedures are combined into a single linkage segment private to the process Linking ... the link pointer and have the form shown in Figure 12 The displacement k is determined by the coding of P and is invariant with respect to the process using P Procedure Call and Return The coding ... entry point in the called procedure segment Thus in establishing the link for an external procedure call, the generalized indirect address placed in the calling procedure's link data points to...
... Vinh (North central region), Can tho and Bac Lieu (Mekong river delta) On the other hand, in winter-spring season rainfall is increasing trend which observed at Bac Giang, Ha noi, Nam dinh, Vinh, ... crop rotation , especially for defining the growing period for Agriculture in the North andin the Mountain regions Absolute minimum temperature Absolute minimum temperature is very important ... mountain in central region (see table 5) Rainfall The situation of rainfall is some what complex depending on locations and seasons In annual and summer season (May - October) small decreasing...
... medicine are undeniable If nothing else had been achieved but two things – in the nineteenth century, the end of bleeding, purging, and leeching as a result of Louis’ studies (Louis, 1835); andin ... handling measurement bias Randomization is the best means of addressing confounding bias, and blinding the means for measurement bias While blinding is important, it is not as important as randomization ... securing information on them” (Miettinen and Cook, 1981) Confounding bias is handled either by preventing it, through randomization in study design, or by removing it, through regression models in...
... decades later The number of men in this sector remained stable over the period, at around 700 000 Women also made gains in both the finance/banking industries andin the defence-related public ... women worked in finance and banking institutions in 1975, the number increased to 450 000 by 1995 The number of men grew only marginally from 425 000 to 480 000 over the same period In defence, ... 1975 and 1995 brought significant changes in the representation of women in Freedonia's work force, according to the graphs In 1975, for example, some 300 000 men and 250 000 women worked in the...
... contributions ARD was involved in the study conception and design, data collection, analysis, revision, editing and manuscript writing HK was involved in the conception and design of study and took an ... active part in the data analysis and results interpretation GO participated to the study conception and design, writing-up and finalization of the manuscript MC contributed to analyze and interpret ... During the open coding phase, all the interviews were read several times, and key words and phrases, incidents and facts in the text were noted Primary codes were extracted The codes and data were...
... disturbances, and could potentially be used to help clinicians identify patients at increased risk An elevated baseline creatinine was associated with a 50% increased risk of ICU-acquired hypernatraemia and ... (1.07 to 1.70) 0.010 *Time-independent (age, baseline creatinine, random glucose) and time-dependent (minimum Glasgow coma scale, glucose level, Acute Acute Physiology and Chronic Health Evaluation ... Calgary and CHR approved this study and waiver of patient consent Data sources Demographic, hospital and clinical data were obtained using the regional ICU patient data warehouse Data sources include...