choose expression from the type pull down menu so you can put the variable framenumber in the frame field
Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
... GATA-binding factor GATA-binding factor GATA-binding factor GATA-binding factor Sox-5 Sox-5 Yin and Yang nuclear factor Y nuclear factor Y E2F, involved in cell cycle regulation E2F, involved in ... Toronto, Ontario, Canada) in both directions The deduced amino acid sequence of the FABP was determined using the algorithm in GENE RUNNER v 3.05 (Hastings Software, Inc.) Nucleotide and amino acid sequences ... Ontario, Canada), fixed in 4% paraformaldehyde for min, in NaCl/Pi twice for three min, and then washed in 1· NaCl/Cit for 20 The fixed tissue sections were immersed in a hybridization buffer containing...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... supplements The coding region of HPV16 E5, an E5 alpha type protein [32], containing a HA-tag at the 5-end terminus and was cloned into the pCI vector (Promega) devoid of the starting methionine Further, ... domain of HPV16 E5 Since the dimer calnexin-HLA is a natural step in the antigen processing route, it can be hypothesized that HPV16 E5 binds to the calnexin-HLA-I complex and that this binding ... blocks further trafficking of the HLA-I complex to the plasma membrane, leading instead to its accumulation in the ER/Golgi of the infected cell A direct binding of E5 to the heavy chain of HLA-I...
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Báo cáo hóa học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient cells" ppt
... supplements The coding region of HPV16 E5, an E5 alpha type protein [32], containing a HA-tag at the 5-end terminus and was cloned into the pCI vector (Promega) devoid of the starting methionine Further, ... domain of HPV16 E5 Since the dimer calnexin-HLA is a natural step in the antigen processing route, it can be hypothesized that HPV16 E5 binds to the calnexin-HLA-I complex and that this binding ... blocks further trafficking of the HLA-I complex to the plasma membrane, leading instead to its accumulation in the ER/Golgi of the infected cell A direct binding of E5 to the heavy chain of HLA-I...
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Prototane - Type triterpenes from the rhizomes
... connected from the spin-system of the 1H-1H COSY and was further confirmed by the long-range correlations in the HMBC spectrum as shown in table All the NMR data suggested the Protostane type of ... The rhizomes of Alisma plantago-aquatica L var orientalis Samuelsson were collected in Tam Dao Mountain, Vinh Phuc Province in January, 2006 and were identified by Dr Tran Huy Thai, Institute ... spectrum and by the spin-coupling of proton H-11 ( 3.88, ddd, J = 5.8, 10.7, 10.7 Hz) [6] The carbonyl group was assigned to C3 from the cross peaks of protons H-23/H-24 and carbon C-3 in the HMBC spectrum...
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Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx
... of the L-hOrn3 a-amino group onto l-haOrn4 Taking into account that the synthetases involved in the biosynthesis of the DKP-inheriting toxins thaxtomin and fumitremorgin also contain a C-terminal ... domain A4 again is predicted to activate l-arginine, displaying 60% identity to the characterized A-domain of MycC Interestingly, A1 and A4 inherit a highly identical (90%) specificity-determining ... periplasmic-binding protein Putative non-ribosomal peptide synthetase MbtH protein Putative ABC transporter transmembrane component ABC transporter protein, ATP-binding component IclR -type transcriptional...
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Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc
... plateau in the spinal cord about 10 days after birth (Fig 5E) To determine the origin of AChE in the spinal cord, the lumbar region of the spinal cord was sectioned and stained with the PRiMA ... expressed in the spinal cord: the PRiMA I transcript decreased slightly after birth, but increased dramatically thereafter and was the predominant form in the adult, the PRiMA II transcript first increased ... Schematic diagram showing the lumbar region of the spinal cord (left) The dorsal horn and ventral horn are indicated The right panel shows PRiMA staining in the lumbar region on the same scale at...
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Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt
... found either in wild -type strains and in mutant strains harboring mutations in the genes encoding enzymes of the biosynthesis and/or the transfer of the O-specific polysaccharide [12,13] The core ... of G was inferred by taking into account the down eld shift of the carbon signal C-6 (63.7 p.p.m., Table 1) indicating its involvement in a glycosydic linkage The HMBC spectrum confirmed the sequence ... contained the significant longrange correlations required for the determination of the sequence and of the attachment points In fact, together with intraresidual long-range cross-peaks, interresidual...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... the neighbor joining method (using PAUP software) from the amino acid sequences of the IL-10 family together with the carp IL-10 gene The numbers indicate the bootstrap confidence values obtained ... to the carp IL-10 sequence The most highly conserved stretch in the IL-10 family is at the C-terminus in the C-helix and at the Nterminus of the F-helix; a similar pattern was also found in carp ... harbored two instability motifs, in comparison to five in human IL-10, in the 3¢-UTR Whether this difference in the number of instability motifs affects translation needs to be investigated in future...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... chain due to the loss of the hydrogen-bonding interaction with the side chain amide nitrogen of Asn181 [39] This may cause less effective substrate binding in the )1 subsite Interestingly, in the ... of the high amount of arginine present in the refolding buffer Even after repeated, extensive dialysis the recombinant hevamine was not retained on the Mono S column Nevertheless, the recombinant ... refold these inclusion bodies The procedure yielded pure protein as judged by SDS/ PAGE The activity of the pure recombinant protein was 80% of that of the wild -type protein in both the lysozyme...
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Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
... related to YRPW motif Ribosomal protein L30 Lysosomal-associated protein transmembrane 4b Fatty acid-binding protein 4, adipocyte Angiopoietin Serine ⁄ threonine kinase Metadherin mftc 19, 46.86 LN54 ... only in the proximal region of the retina, whereas fabp3 transcripts were distributed in several structures, including the diencephalon, hindbrain, spinal cord, and somites (Fig 5A) At 24 hpf, the ... protein tyrosine kinase Mapping panel Gene symbol Chromosomal location Mapping information derived from the merged genetic map at the ZFIN website: http://www.shigen.nig.ac.jp:6070 b Mapping information...
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Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... Gly164 in (A) (C) The deduced amino acid sequence of LdUSP The DNA binding domain (DBD, C-region) is underlined The ligand binding domain (LBD, E ⁄ F-region) is underlined with dashes The sequence ... against L decemlineata, suggesting that the receptor-binding of DBHs in L decemlineata is different to those in C suppressalis and S exigua The aim of this study is to examine the SAR of ecdysone ... demonstrated the temporal expression pattern of LdEcR in the integument of 4th instar larvae As shown in Fig 2A, the EcR message steeply increased at day 4, then remained at the high expression...
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Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc
... ranges from 87.5 to 59, the latter value pertaining to human L chains The most similar to the T bernacchii constitutive chain are the H2 chain from S gairdneri (87.5%) and the M chains from S ... these findings, the fluorescence intensity decreases and the emission wavelength shows a modest shift towards the red, indicating further exposure of the W93 residue to solvent Interestingly, the ... within the four-helix bundle of the L chains between K62 and E107 and thought to play a special role in conferring thermal stability [20]; in the T bernacchii M chain, just as in the human H chains,...
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Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt
... adaptation by causing dispersion of melanin pigment granules in skin melanophores resulting in darkening of the skin [23] POMC is the major cargo protein in this cell type and during adaptation ... panels), suggesting that the expression of the GFP–PrPC fusion protein in the intermediate pituitary is coregulated with these proteins The POMC and p24d1 ⁄ protein levels were similar in the ALs of ... directing expression of the fusion protein specifically to the Xenopus melanotrope cells, leaving the integrity of the regulation by the hypothalamic neurons intact We studied for the first time in...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
... curdlan or laminarin After binding of the carbohydrate to the receptor, the expression of the gene coding for the binding protein increases In turn, the potential scavenger molecule, a fibrinogen-related ... localization, expression of the (1fi3)-b-Dglucan-binding protein in the cells of the pinacoderm, can be expected for fibrinogen or the related molecules, e.g ficolins, which are involved in host defense ... against the highly conserved region within the (1fi3)-b-D-glucan-binding proteins; in the b-1,3-glucanbinding protein from the black tiger shrimp, Penaeus monodon (accession number AF368168-1) the...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... depicting the phylogenetic relatedness of gcGLUT to the known GLUT1– GLUT4 proteins from fish, avian or mammalian sources The protein sequences obtained from the GenBank/EMBL/Swissprot databases include: ... encoding these domains and that there is a high selective pressure to maintain the arrangement of these exons In mammals, hypoxic stress is known to increase GLUT1 and GLUT3 expression in specific ... (3) the serine/threonine residues at positions 298 and 299 in loop (Fig 2) that are involved in conformational change of the GLUT protein during transport [25] To date, cDNAs of five GLUT isotypes...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... NifL-protein are located in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal domain of NifL might enter the lipid bilayer and contact the quinones dissolved ... tetracycline-resistance cassette (derived from the MiniTn5 [28]) was inserted and the resulting interrupted genes were cloned into the suicide vector, pKAS46 These constructs were then introduced into ... transduction into the parental strain After introducing nifLA and nifA into plasmids, the resulting strains were grown anaerobically under nitrogen-limitation with glutamine as the sole nitrogen source...
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Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
... for the major cross-linking reaction in P polycephalum Furthermore, the result of the Northern blotting indicates that a single species of transcript arises from the genomic locus corresponding ... around the GTPbinding region, catalytic site, and Ca2+-binding region are highly homologous to the corresponding regions of the human TGase and the other invertebrate TGases (Fig 3) The eight amino-acid ... terminus that is required for binding to the plasma membrane, thereby being involved in the formation of the corneum [47] Additional amino-terminal residues of TGase include the sequences that are post-translationally...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... sieboldina agglutinin [21], the exception being the ricin B1 domain which has two sugar-binding sites in the same molecule [22,23] Based on these structural features, R -type lectins generally contain ... obtain the Kd more precisely for the sugar-binding site in the slow exchange regime However, the Kd of the a sugar-binding site could not be calculated using nonlinear regression fitting to the binding ... concentration to the binding isotherm describing the binding of one ligand molecule to a single protein site using the Solver function of 15 EXCEL 2002 The weighted average of the H and N chemical-shift...
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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
... successfully used in the studies of the respective proteins [34,35] Applying this approach for the production of the first recombinant protein of invertebrate origin, the 2-5A synthetase from the sponge ... synthesizing activity of the recombinant porcine 2-5A synthetase (A) and N-terminally His-tagged recombinant 2-5A synthetase from G cydonium (B) The products formed from ATP during a h synthesis ... of determining the absolute values of these parameters may be low, the data show an increasing trend in the specific activity depending on the number of nucleotides per protein molecule In order...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... b-tubulin chains from the giant amoeba R filosa were shown to remain soluble when expressed in E coli following their interaction with the prokaryotic chaperonin GroEL/ES [29]; the resulting soluble ... to the difference in the isoelectric point of b-T1 and human b5, calculated from their respective primary sequences (4.55 and 3.91, respectively, determined using the WINPEP software [39]) Finally, ... 4–5 °C [23] In contrast to other unicellular eukaryotes, in which the tubulin pool is generally represented by a single isotype, in E focardii, three distinct isotypes of b-tubulin have been...
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