chemokinesis and chemotaxis in marine bacteria and algae
Marine Chemical Ecology - Chapter 14 pptx
... extension in a marine mollusc, Science, 248, 356, 1990 89 Crisp, D J., Factors in uencing the settlement of marine invertebrate larvae, in Chemoreception in Marine Organisms, Grant, P T and Mackie, ... by tumbling episodes When moving in a direction interpreted as undesirable, tumbling becomes more frequent; after each tumble, swimming begins anew, and, since tumbling results in a random reorientation ... just the standard 20 protein-forming amino acids afford — 20 n, with the exponent n indicating the chain length of a peptide) In both internal and external environments, nucleotides and amino acids...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
... Active-site-binding ligands (e.g amino-oxyphenylpropanoic acid (E)-cinnamate, o-tyrosine) protected the R glutinis PAL from inactivation by the three proteinases, and peptide-bond cleavage by trypsin and ... homotetrameric ‘Tyr-loop -in bacterial models for P luminescens PAL (PlPALin) and S maritimus PAL (SmPALin) were constructed and refined in Swiss-PdbViewer by fixing the bumping side chains followed by ... Constructing the ‘Tyr-loop-out’ variants of the bacterial PALs The ‘Tyr-loop -in models PlPALin and SmPALin were modified by replacing residues 50–85 (PlPALin) and 34–68 (SmPALin) with the corresponding...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... contributes to this interaction, but is not absolutely required; and (c) that other regions of 4E-BP1 are also involved in binding raptor Interestingly, as noted above, mutating the RAIP and TOS motifs ... for the increased phosphorylation induced by insulin We therefore first replaced the isoleucine by the other branched-chain residues, valine and leucine These 4E-BP1 variants were expressed in HEK293 ... motifs in 4E-BP1 V H Y Lee et al ‘functional’ residues (valine, alanine, proline) indicates that a range of side-chains are tolerated here Interestingly, the least effective residue tested (arginine)...
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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt
... analyzed (Fig 2B) In order to investigate whether CEdG has the potential to accumulate in genomic DNA of nongrowing cells, HSC-T6 cells were kept in minimal medium containing 1.5% fetal bovine serum ... shown in Table The amount of CEdG formed during the DHA incubation of the vector pGL3 Control increased with both incubation time and quantity of glycating reagent The reaction mixture containing ... cells (HSC-T6) were incubated in growth media (MEM; Biochrom, Berlin, Germany) with Earle’s salts containing 20% fetal bovine serum (Biochrom) and 1% penicillin ⁄ streptomycin solution (10 000...
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expression of recombinant genes in eukaryotic systems
... using T7 RNA polymerase in a buffer containing 7.5 mM ATP and GTP, 5.6 mM UTP and CTP, and 1.875 mM biotin-6-UTP and biotin-ll-CTP The in vitro-transcribed RNA is then ethanol precipitated and ... thus standardizing and easing the washing process The D N A microarrays can also be washed by immersion in a beaker containing a stir bar and a buffer at the appropriate concentration and temperature ... Proteins Using Baculovirus-Infected Insect Cells Cell Culture Maintenance For initial transfection and selection of recombinant virus and plaque assays, we have obtained the best results using IPLB-Sf21-AE...
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Medsoft, deciphering principles of transcription regulation in eukaryotic genomes
... domains 19 DNA-binding Domains In accord with the sequence and structure of DNA-binding domains, regulatory proteins are often grouped into class The goal function of the DNA-binding domain rules ... affinities for core promoters (e.g., TBP binding to consensus and nonconsensus TATAs; TFIIB binding to a consensus vs a degenerate BRE; TFIID binding to an Inr-containing vs Inr-less promoter) Instead, ... contains one domain on the amino terminus nearby to the DNA binding domain and another on the carboxyl terminus [12] Organization in a domain is substantially flexible For example, deleting analysis...
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Báo cáo y học: "Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid"
... Zn Vitamin B1 Vitamin B2 Vitamin B6 Vitamin B12 Vitamin C Folic acid Nicotinamide Biotin Panthenol Vitamin A Vitamin D3 Vitamin E Vitamin K pH Osmotic pressure ratio to physiological saline NP1 ... standard strain ATCC11778 and clinical isolates (H1, H2, H3) of Bacillus cereus, and the standard strain ATCC10231 and clinical isolates (N6, N7, N8) of Candida albicans Experiment 2: The standard ... strain and or clinical isolates were used for each microorganisms; the standard strain ATCC6538 and clinical isolates (N1, N2, N3) of Staphylococcus aureus, the standard strain ATCC13880 and clinical...
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Inactivation of microorganisms in untreated water by a continuous flow system with supercritical CO2 bubbling
... method for inactivating microorganisms in drinking water In addition, we aim to investigate the effect of the SC-CO2 treatment with regard to structural damage and enzyme inactivation in E coli ... the inactivation of coliform bacteria in untreated water is shown in Figure The coliform bacterial count in the untreated water decreased almost linearly with increasing CO2/sample flow rate in ... apparatus for a continuous flow treatment with SC-CO2 bubbling Measurement of surviving bacteria The coliform and total bacterial counts in the untreated water were determined using the method of...
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Evaluation of the Characteristics of Microorganisms that Contribute to Denitrification in the Paddy Drainage Treatment Apparatus by Quinone Composition Measurement
... Akita-Komachi Rice, and the planting rate was 0.30 m (ridge pitch) and 0.15 m (planting pitch) The paddling and transplanting were conducted on June and June 8, and the inundation began on June in 2007 A ... analysis and does not require any biological and biochemical knowledge or skills Thus, the quinone profiling has high affinity with analysts Moreover, quinone composition used in quinone profiling ... will lead to the finding of paddy characters accomplishing high denitrification activity REFERENCE Burlingame G A., Suffet I H and Pipes W O (1986) Predominant bacterial genera in granular activated...
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Microorganisms Associated with Volatile Organic Compound Production in Spoilt Mango Fruits docx
... used in cosmetics, paints and coatings, lubricants and in the food industry (Bódalo et al., 2005) They are useful chemical intermediates in the synthesis of fine chemicals and pharmaceuticals ... constant flow rate of 0.8 ml/min The oven temperature was programmed at 60 oC for 5min, followed by an increase (held for min), and finally at 100C/min to 280 oC (held for 10 min) The temperature of ... during harvesting, transit or storage periods or when warmer fruit are washed in cooler contaminated water (Kucharek and Bartz, 2000) Early detection of spoilage would be advantageous in reducing...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... the protein during the docking to the binding site on the ribosome or other ligands Strikingly, the second most flexible part of the protein (if one does not take into account the N-terminal region ... implicated in stop codon recognition [5–14] The role of the middle (M) domain will be described in detail below The C domain of eRF1 interacts with the C domain of eRF3 [15–18], and the binding of ... yeast eRF1 in vivo [3], and of Es coli RF2 both in vivo and in vitro [31,32] For instance, GAQ mutants of both RF1 and RF2 are four to five orders of magnitude less efficient in the termination reaction...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
... Dm-eIF4B-L in Drosophila cell cultures and in developing tissues provided interesting insights into the function of eIF4B In mammalian cell cultures, a 50-fold overexpression of eIF4B resulted in the inhibition ... and eIF4F are involved in the scanning process complementing the role of eIF1 and eIF1A, and that in the presence of eIF4F, eIF4B and eIF4A, scanning is less dependent on eIF1 and eIF1A [60,61] ... 20 in · NaCl/ Pi and, when indicated, incubated for with Alexa 488-conjugated phalloidin (1 : 100) in · NaCl/Pi Fluorescent signals were acquired and images analyzed using a ´ 2926 G Hernandez...
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Bioavailability and toxicity of cd to microorganisms and their activities in soil
... microbial biomass in the soil (McCann and Cullimore, 1979) and are involved in maintaining soil fertility and oxygen production (Bold and Wynne, 1978) Mineralization of algae releases nutrients which ... wastewater and other contaminated resources through sorption and accumulation has been gaining importance in recent years 2.2 Bacteria and other microbial populations in soil Bacteria and fungi ... being an enzyme inhibitor, can have deleterious effects on membrane structure and function by binding to the ligands such as phosphate and the cysteinyl and histidyl groups of proteins (Collins...
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Báo cáo khoa học: Autophosphorylation of heme-regulated eukaryotic initiation factor 2a kinase and the role of the modification in catalysis doc
... phosphorylations inhibit protein– protein interactions between isolated NTD and KD domains, leading to the suppression of intramolecular interactions (between N-terminal and C-terminal domains) within the ... protein rearrangements, including intramolecular protein–protein interactions, are required for heme sensing because the axial ligands of the heme, His119 ⁄ His120 in the N-terminal domain and ... protein during heme shortage HRI is composed of an N-terminal domain (NTD) (amino acids 1–144) (Fig 1, red area) and a C-terminal kinase domain (KD) (amino acids 145–619) (Fig 1, green area) including...
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Báo cáo khoa học: Protein folding and disulfide bond formation in the eukaryotic cell pptx
... exciting area of research forwards into the future Indeed, obtaining accurate measurements of the concentrations, distributions and flux of all the main players involved in protein folding in the ... alongside oxidative and reductive pathways of protein folding, and thus functions to help maintain balanced ER redox conditions, are sure to emerge in the coming years Protein targeting and disulfide ... between ricin A retention in the ER and its ubiquitination, and hence proteasomal degradation, in the cytoplasm [31] Protein turnover Oxidative protein folding in the cytoplasm itself is rare Instead,...
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beneficial indigenous microorganisms and bionutrients in natural farming
... microorganisms and enzymes, on energy chi, balance and harmony, of opposites, yin and yang Living soil is the basis of soil fertility Soil, living soil is the alpha and omega, the beginning and end And ... growth hormones and trace elements, and good source of nitrogen and potassium? Check out the kinds of materials you can ferment and use to induce growth, flowering and fruiting Fish Amino Acid As ... power elements in Nature? How we apply the principle of “farming with air, water and sunlight?” In Biointensive mini-farming, one of the basic requirement in the preparation of planting beds is...
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Báo cáo hóa học: " Nanofluid bioconvection in water-based suspensions containing nanoparticles and oxytactic microorganisms: oscillatory instability" potx
... regarding the development of the model, performing simulations, writing and revising the paper and approving the final manuscript Competing interests The author declares that he has no competing interests ... oscillatory instability boundary is obtained by eliminating Ra from the two coupled equations resulting from taking the real and imaginary parts of Equation 54 and solving the resulting equation ... the reasons why this is challenging is because although inducing mixing at the macroscale is easy and can be achieved by stirring, inducing and controlling mixing at the microscale is difficult...
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GENETIC DIVERSITY IN MICROORGANISMS pptx
... as biomining (Rawling, 2002) Biomining is becoming increasingly important in mining because of its lower and containable pollutant outputs comparing to thermal processes Efficacy in biomining environments ... halophilic, and many useful enzymes applied in the detergent industry (washing powders), the textile industry, and other processes were obtained from bacteria growing in saline alkaline lakes Halophilic ... Ammoniaoxidizing archaea are determined to thrive in various habitats including marine and fresh waters, hot/thermal springs, soils, and wastewater treatment systems (Youa et al., 2009) The fascinating...
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INTERRELATIONSHIPS BETWEEN MICROORGANISMS AND PLANTS IN SOIL doc
... that growth inhibition was linked to binding of the bacteria to the hyphae, thus indicating that a lectin-type interaction is probably involved (Nelson et al., 1986) This interaction may not however ... aspects of grain plant response to inoculation INTRODUCTION Obtaining strains capable of forming an effective symbiosis with various species of host-plant is the main task of nodule-forming bacteria ... in a single mode It should be possible to isolate antagonists with one or more antagonist actions, and then introduce the others by genetic engineering with recombinant DNA or protoplasting and...
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Scientific report: "Identify some microorganisms that cause food poisoning in water use in the private slaughterhouses in Hanoi, Vietnam" pot
... condition During slaughtering and subsequent meat processing, the water used for processing becomes a contaminating source Follow the standard of Ministry of Agriculture and Rural development, in the ... hours after infection, including fever, abdominal pain, diarrhea, nausea and sometimes vomiting The results of Salmonella isolation in water using in slaughterhouse were represented in table Salmonella ... then enriched by placing in selenite cystine and tetrathionate broths (9 ml each) and incubating at 42 0C and 37 0C respectively for 24 h Samples were inoculated onto Xylose-lysine-desoxycholate...
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