... Recent studies demonstrated that fucoidan has significantly important biological effects on natural killer cells [13 ], hematopoietic stem cells [6], endothelial cells [11 ], and DCs [10 ] Although the ... contains two novel proteins related to baculoviral inhibitor of apoptosis proteins Cell 19 95, 83, 12 43 -12 52 17 Santini V, Giles FJ The potential of amifostine: from cytoprotectant to therapeutic ... together, our results demonstrate that fucoidan increases BMC viability following irradiation These effects may be the result of apoptotic inhibition in irradiated BMCs Fucoidan treatment alters...
... magnitude (the maximum magnitude of the measurement area of the operated knee divided by that of the intact cartilage of the opposite, nonoperated knee; %MM) was used as a quantitative index of the ... R558 Arthritis Research & Therapy 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 R559 Vol No Hattori et al human cartilage Clin Biomech (Bristol, Avon) 2004, 19 : 213 - 216 Hattori K, Takakura ... as the maximum magnitude of the measurement area in the operated knee, divided by that of the intact cartilage of the opposite, nonoperated knee *P < 0.05 on the nonparametric Mann–Whitney U test...
... down-regulated genes annotated in the GO system (not shown) There were 348 genes in the list for the 764 up-regulated genes and 733 genes in the list for the 1, 910 down-regulated genes List hits shows the ... Tcells sensitive to apoptosis [ 31] Together with their results and our finding that the ATM signaling pathway is repressed in the immunoregulatory networks of BMMC, we suggest that in RA patients, ... results suggest that the overexpression of genes that take part in the antigen presentation pathway and IFN signaling contribute to the pathogenesis of RA Our results also suggest that the underexpression...
... The data are expressed as the ratio of the mRNA copy numbers to those of β-actin Effect of treatment with methotrexate (MTX) or oral steroids (Steroid) was evaluated by Welch's t test Horizontal ... quantitative PCR The data are expressed as the ratio of the mRNA copy numbers to those of β-actin The data are representative of two different experiments ure 7, treatment of bone marrow CD34+ cells ... ELISA Statistical significance was evaluated by Wilcoxon's signed rank test their capacity to differentiate into fibroblast-like cells upon stimulation with TNF-α The data suggest that the increased...
... on the platform, the background image was taken under the lights of an illuminator http://www.medsci.org Int J Med Sci 2 011 , Then, the illuminator was turned off, and the image of light emitted ... intravenous transplantation of MSCs is whether the MSCs can migrate into and proliferate in the target tissues, which is the basis of therapeutic effects of MSCs In adults, MSCs remain the potentials ... necrosis In the intravenous transplantation of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can...
... (SpotsNo 4, 64, 10 3, 10 8, 11 4, 12 1, 12 3) (Table 3) The majority of these proteins have unspecific or unknown functions or they are clearly related to tissues and not to hematological cells [18 -23] As ... competing interests Authors' contributions 10 11 Note 1 %T [19 ] Acknowledgements Competing interests GH, AL, DN, and JP carried out the 2D electrophoresis and all other experimental work PS, ET, ... Annexin Patient A 12 9 76 77 67 #02-05p 12 9 76 77 67 #02-02b Figure Detail of the two-dimensional patterns of patient A Detail of the two-dimensional patterns of patient A Detail of the two-dimensional...
... and the ATMP, the product possesses no antimicrobial activity under the conditions of the test, and the sterility may be then carried out without further modification Data Analysis For the endotoxin ... limit represent the lowest bacterial concentration in the inoculums that the system can evidence The specificity of the system represent its ability to detect the single micro-organism in the ... inoculums and the detection limit represent the lowest micro-organism number in the sample that the system can detect The robustness of the system represent its ability to obtain identical results when...
... in order to investigate the attitude of the engrafted stem cells to the hosting myocardium [26] The number of treated patients with the combined approach so far is limited and current evidence ... application of stem cells into the failing heart with multiple targeted injections following device insertion Note the outflow graft (1) connected to the device (2) which has been implanted into the ... engraftment, and poor survival of the implanted cells in the host myocardium limit their potential for a significant clinical benefit Hybrid combination therapy with LVAD and stem cells implantation;...
... vitro and implantation in vivo http://www.josr-online.com/content/4 /1/ 5 10 11 12 13 14 15 16 17 Competing interests The authors declare that they have no competing interests 18 Authors' contributions ... endothelial cells Our data showed that the addition of HA elevated NO production in comparison with the HA-free PCL scaffolds, suggesting that HA particles promoted functions of the endothelial cells ... biocompatible and osteoconductive It suggests that the addition of HA to PCL will improve the biocompatibility and osteoconductivity of the polymer [12 -14 ] However, the precise dose-response relationship...
... It should be pointed out that the AFM tip is going to be contaminated at the first touch and continue with the following touches, and this contamination can influence the next interaction of the ... between DCs and the latter [17 -19 ] Therefore, we further examined whether BMDCs treated with LPS were of a functional feature of DC maturation The amount of IL -12 in culture supernatants of BMDCs ... the top central part of thecells Statistical analysis Numerical data obtained from each experiment were expressed as mean ± SD, analyzed by SPSS 10 .0 statistical package The Student’s t test...
... We thus may postulate that TNF-α is the key molecule at the onset of RA pathogenesis that induces or contributes to modifying the characteristics of the MSC, which then R1 313 act to favor the ... the data DN participated in the design of the study and the analysis of the data, and wrote the manuscript All authors read and approved the final manuscript 16 17 18 19 20 References 10 11 12 ... the data FC procured the samples of arthritic synovium TR helped with the biostatistical analysis JS participated in the analysis of the data CJ participated in the design of the study and the...
... functional activities that could contribute to rheumatoid inflammation Among these activities are their ability to promote antibody production by B cells, the capacity to protect lymphocytes ... increased by co-culture with B cells IL -1 and TNF were only detected in the culture supernatants after co-culture with B cellsThe effect of co-culture with B lymphocytes on the secretion of cytokines ... apoptosis, the ability to secrete large amounts of cytokines and chemokines that could promote the accumulation and activation of lymphocytes and monocytes, and their unique capacity to promote the...
... Competing interests The authors declare that they have no competing interests Authors' contributions LX had full access to all data in the study and takes responsibility for the integrity of the data ... vitro They speculated that this could increase the ability of OCPs to enter the bloodstream [23] It has been reported recently that RANKL-stimulated osteoclastogenesis promotes the mobilization ... whether they could differentiate into osteoclasts, as described previously [4] These treated cells were fixed and stained for tartrateresistant acid phosphatase activity to identify osteoclasts...
... to rituximab Taken together, these results suggest that the BM B cells are more resistant than PB B cells to antiCD20 mAb depleting therapy in patients with RA Rituximab preferentially depletes ... GK participated in the design and conduction of the study and drafted the manuscript PS participated in the design of the study, performed the statistical analysis of the data and drafted the manuscript ... activity scores that include twenty-eight-joint counts Development and validation Page of (page number not for citation purposes) Arthritis Research & Therapy 11 12 13 14 15 16 17 18 19 20 21...
... University, 211 100, P.R China Page of 10 10 11 12 13 14 15 16 17 18 Authors’ contributions YL, DZ and YZ carried out the main experiment and drafted the manuscript FZ and DZ conceived of the study ... than those in the control group, there was no significant difference between the two groups These results indicated that PI3-K pathway was of importance for the anti-apoptotic role of simvastatin ... pretreatment with 50 n M wortmannin, a PI3-K inhibitor, diminished the anti-apoptotic effect of simvastatin (Fig 7) The cell viability detected by MTT assay was significantly higher in simvastatin...
... manuscript 12 13 14 15 16 17 18 Competing interests The authors declare that they have no competing interests Received: 11 October 2 010 Accepted: February 2 011 Published: February 2 011 19 References ... with the cultures of days In the CTRL/7d and FeProt/7d groups, we plated half the amount of cells plated in the CTRL/3d and FeProt/3d groups but thecells approached confluence at days, and the ... measurements of the group pre-treated with mitomycin C showed a sustained proliferation rate of 6.9% (data not shown) We believe that it is very important that a significant fraction of thecells retain...