cd33 and cd11b subsets to be both hla drlow and lineage
Báo cáo sinh học: " Transcription and translation of human F11R gene are required for an initial step of atherogenesis induced by inflammatory cytokines" doc
... exposed to proinflammatory cytokines: time and dose-response The expression of F11R mRNA was examined both in arterial HAEC and venous HUVEC following their exposure to the pro-inflammatory cytokines ... diluted in ethanol to a mM (100X) stock solution All stock solutions were diluted in culture media to 1X concentration prior to experimentation HAEC and HUVEC were grown to confluence and then treated ... exposed to proinflammatory cytokines TNFa and IFNg: dose response Endothelial cells, HAEC and HUVEC in culture, were treated with different concentrations of TNFa (0.5 to 1000 pM; to 2000 units) and...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" doc
... cross-talk between osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand-receptor system that directly regulates osteoclast differentiation and bone resorption, and both are ... produced and secreted by osteoblastic lineage cells [13,14] On the one hand, RANKL binds to RANK, which is expressed on osteoclast progenitors, and leads to osteoclast activation On the other hand, ... osteoblasts, neurotransmitters, and the OPG/RANKL/RANK system have to be undertaken to gain a better understanding of the multifactorial process of aseptic loosening and possible therapeutic options...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" pot
... cross-talk between osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand-receptor system that directly regulates osteoclast differentiation and bone resorption, and both are ... produced and secreted by osteoblastic lineage cells [13,14] On the one hand, RANKL binds to RANK, which is expressed on osteoclast progenitors, and leads to osteoclast activation On the other hand, ... osteoblasts, neurotransmitters, and the OPG/RANKL/RANK system have to be undertaken to gain a better understanding of the multifactorial process of aseptic loosening and possible therapeutic options...
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báo cáo hóa học: " Human brain endothelial cells endeavor to immunoregulate CD8 T cells via PD-1 ligand expression in multiple sclerosis" pdf
... added to the upper chamber (1 × 10 cells per Boyden chamber) and allowed to migrate for 24 hours across HBECs FITClabeled BSA (50 μg/ml; Invitrogen) was concurrently added to the upper chamber and ... vitro BBB model HBECs were plated to the upper chamber of a Boyden chamber and then inflamed Activated CD8 (A, B) and CD4 (C, D) T cells were added to the upper chamber and allowed to migrate for ... increased levels of PD-L1 and PD-L2 in response to pro-inflammatory cytokines Such PD-1 ligand expression contributes to the capacity of HBECs to reduce the migration and activation of human T...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... was loaded into a 96-well plate and examined using a luminometer (Victor3; PerkinElmer, Finland) and a fluorescent plate reader with excitation and emission wavelengths of 485 nm and 535 nm, respectively ... addition, phosphorylation of JNK and p38 MAPK has been shown to play a role in cellular differentiation and inflammatory responses [17,29,35] Antioxidants have been shown to attenuate the activation ... peroxidation, and inhibited by antioxidants such as β-carotene and superoxide dismutase [14,27,55] Elevated levels of LPO have been linked to cell injury such as the increase in permeability to ions and...
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Báo cáo y học: " Effect of recombinant IL-10 on cultured fetal rat alveolar type II cells exposed to 65%-hyperoxia" pps
... that balance between pro- and anti-inflammatory cytokines is shifted by a combination of an increase of pro-inflammatory cytokine and a stronger decrease of an anti-inflammatory cytokine rather ... proliferation and VEGF production, and generates an imbalance between pro- and antiinflammatory cytokines, and addition of rIL-10 reduces cellular necrosis and IL-8 production and promotes cell ... According to the former data showing that 65%-hyperoxia induces increased cell death and decreased VEGFrelease and cell proliferation and generates an imbalance between the pro-inflammatory cytokine,...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... using a combination of flow cytometry and immunocytochemistry (Figure 4) Using antibodies to CD133 and NF-κB on primary tumor cultures demonstrated that both progenitor and stem cells expressed NF-κB ... flow cytometry and immunocytochemistry Validation of selected genes by flow cytometry and immunocytochemistry (a) Flow cytometry analysis of prostate cancer cells co-stained with antibodies to CD133 ... [36]) With respect to prostate cancer, Wnt signaling has been linked to progression to androgen-independence and bone metastasis [37,38] Extracellular matrix-receptor signaling and the focal adhesion...
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báo cáo hóa học: " Increased levels of HMGB1 and pro-inflammatory cytokines in children with febrile seizures" pot
... shown to be a key mediator of inflammatory diseases HMGB1 is a nuclear protein that triggers inflammation, binds to lipopolysaccharides (LPS) and IL-1, and initiates and synergizes with a Toll-like ... cytokine levels and laboratory findings between controls and febrile seizure patients The Spearman’s rank correlation coefficient was calculated to detect significant correlations between cytokine ... Figure Correlation between serum cytokine levels in seizure patients (A-D) Correlation between serum levels of IL-1b and HMGB1 (A), IL1b and IL-6 (B), IL-1b and TNF-a (C), and IL-6 and TNF-a (D) in...
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Motility and alignment of human umbilical vein endothelial cells (HUVEC) on 3d scaffolds
... station The system has been designed to be compatible with Si wafers up to 6” This new focusing system is able to produce proton beams down to a sub-100 nm spot size, which can be used for maskless ... must be followed And all solutions and equipment coming into contact with living cells must be sterile, and aseptic technique should be used accordingly 27 3.2 Facilities and Methods Used to Record ... Multiplier (CEM) detector, for beam induced secondary electrons The two types of detectors are used to monitor the proton dose written into the resist Figure 2.3: Interview of P-beam exposure station...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization of BECs and LECs ... conformed to guidelines approved by the Institutional Animal Care and Use Committee of the University of Tokyo MAPK p-Akt Akt BECs LECs Fig Isolation and characterization of mesenteric BECs and LECs ... Implications for tube formation-based assays and transfection assays of tsA58T Ag-expressing ECs We also examined whether the cells formed tube-like structures on collagen gel Both uterine BECs and LECs...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... probes shown in Table and Fig Arrows indicate the bands that are interacting with these probes All the lanes have labelled probes The adding of extracts and cold probe are indicated in the top ... with SalI and SmaI (utilizing the SmaI contained in the pBluescript vector) and subcloned into the luciferase reporter vector pGL2-Basic using the SmaI and XhoI sites in this vector to construct ... representing this promoter fragment was subcloned into PCR2.1 and transferred into pGL2-Basic using the KpnI and XhoI sites in the vector and KpnI and SalI sites in the PCR2.1 clones of the promoter...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... acid into prostaglandin H2 [8,9] To date, two distinct COX isoforms, COX-1 and COX-2, have been identified and characterized, and an alternative splice variant of COX-1, termed COX-3 has been cloned ... majority of studies, cPLA2-a has been reported to translocate to the nuclear periphery and structures within the cytosol, sites presumed to be the nuclear envelope, ER and Golgi apparatus These include ... cells has been reported to occur to sites referred to as the ER, Golgi and nuclear membrane [26,27] However, in very few of these cases have direct double-labelling studies been performed to confirm...
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Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt
... 102b Percent loss from ‘Before’ to ‘After’ cell lysis is 2–4% b Percent loss from ‘Before cell lysis’ to ‘After membrane solubilization’ is 11–15% a and 2-mercaptoethanol and subsequent Coomassie ... namely before and after cell lysis and after membrane solubilization, we measured the acetylcholinesterase activity and the protein concentration, so as to verify the percentage of loss between ... recombinant AChE standard (4.5 lg of protein per lL of sample) and human erythrocyte AChE standard (0.06 lg of protein per lL of sample), with or without protein dithiothreitol and 2-mercaptoethanol reduction...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt
... [16], and some tumor cell lines such as B16 melanoma [17] and P19 embryonal carcinoma cells [18] Both GSK3b and p53 have been shown to localize to mitochondria GSK3b was localized to the mitochondria ... energy metabolism and biogenesis have not been addressed Mitochondrial biogenesis is highly orchestrated, and involves signal cross-talk between the nucleus and mitochondria leading to the coordinated ... receptor-c coactivator (PGC)-1a and PGC-1a-related coactivator (PRC), control the expression of nuclear-encoded OXPHOX genes and transcription factor A mitochondria (TFAM), transcription factor...
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Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf
... concomitantly to a variety of proinflammatory mediators, especially AGEs and LPS It has been demonstrated that both AGEs and LPS play key roles in the development of inflammatory diseases by stimulating cytokine ... proinflammatory cytokine ⁄ chemokine production, which requires both mitogen-activated protein (MAP) kinase and nuclear factor-jB (NF-jB) activation Results AGE-human serum albumin (HSA) and LPS ... AGE-HSA and LPS amplified inflammatory response Furthermore, blockade of both MAP kinases and NF-jB with a combination of inhibitors completely abolished AGE-HSA and LPS costimulated IL-6, IL-8 and...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
... potent vasoconstrictor and growth promoter of vascular smooth muscle cells via the ETA receptor, and because vesicular fusion and release has been shown in numerous cases to be triggered by Ca2+, ... NiCl2 (1 mM) and CdCl2 (1 mM) and the Na+/Ca2+-exchanger-specific inhibitor 2¢,4¢-dichlorobenzamil (0.1 mM) were included in HBSS/ 20 mM Hepes and allowed to act on the HUAECs for 20 before the ... inhibitor Ro-31-8425, lM Na+-channel inhibitor tetrodotoxin, 500 lM mitochondrial ATP-sensitive K+ [mitoK(ATP)] channel inhibitor 5-hydroxydecanoate, or with 50 lM Na+/K+/2Cl– cotransporter inhibitor...
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báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf
... cells expressing CEACAMs CEACAM1 and CEACAM6 have been reported to present selectin ligands to CD62E (ELAM-1, E-selectin) on endothelial cells [23], and appear to be involved in angiogenesis [9,16,28,100] ... unresponsive to CD66b, CD66c, and CD66de mAbs, but they remained responsive to both CD66ae and CD63 mAbs (Fig 2, panel E) Discussion While it has been shown that CEACAM-1, -8, -6, and -3 can each ... responsiveness to the unrelated membrane protein CD63 In contrast, neutrophils desensitized to CEACAM-8, -6, and -3, were still responsive to both CEACAM-1 and CD63 Thus, CEACAM-1 appears to have a...
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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
... TMEV and the possible receptors involved by using antagonists of the classical cannabinoid receptors, CB1 and CB2, antagonists of the vanilloid receptor TRPV1 and inhibitors of PPAR-g receptors; ... sodium pyruvate and 1% antibiotic penicillin and streptomycin (all from Gibco, Scotland, UK) and were maintained under standard cell culture conditions at 37°C and 5% CO2 One hour before experiments, ... FBS/phenolred-free DMEM and 200 μl of 0.45% albumin conjugated to Evan’s blue dye were added to the bottom and the top of the well, respectively and incubated at 37°C for 30 Absorbance of the bottom medium...
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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
... due to HA embedding provided more anchorage for cell process and spreading, adhesion and orientation Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and ... complicated and functional bone tissue, and an appropriate vascular bed to support the metabolic needs of new bone tissue We have been developing a composite scaffold consisting of PCL and HA to enhance ... vivo, which had been elucidated in PCL and HA have been shown non-toxic and non-mutagenic biomaterials [11,21-24] In particular, the biocompatibility of HA due to its similarities to natural bone...
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