ca2 activity of f508del cftr er retention in cf cells
Báo cáo y học: "Abnormal spatial diffusion of Ca2+ in F508del-CFTR airway epithelial cells" pptx
... miglustat induces a profound modification of IP3R Ca2+ dependent activity in CF cells Consequence on IP3Rs Ca2+ activity of F508del- CFTR ER retention in CF cells Finally, we used cells derived from CF- KM4 ... Pharmacology of IP3R response of local uncaging of caged Ca2+ in CF1 5 cells in absence of extracellular Ca2+ Pharmacology of IP3R response of local uncaging of caged Ca2+ in CF1 5 cells in absence of extracellular ... http://respiratory-research.com/content/9/1/70 F508del- CFTR correction in CF- KM4 cells restored histamine ER Ca2+ release compared to non CF MM39 cells Figure F508del- CFTR correction in CF- KM4 cells restored histamine ER Ca2+ release...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... Mutations affecting the activity of ricin A chain (Eur J Biochem 271) 155 Creation of ricin A chain variants Ricin A chain variants containing single amino acid substitutions were generated by PCR ... ricin R213A and ricin R213D towards Vero cells Vero cells were challenged with increasing concentrations of toxin at 37 °C for h Remaining protein synthesis after this time was measured by the incorporation ... majority of the interactions that were seen in the first model are maintained However, the interaction between G4 and Asn122 can no longer be made as G4 is in an altered position making a continuous...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... binding of ligands on the iron of Fe(porphyrin))13G10 complexes: (A) binding of H2O2 on the iron of 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding ... packed against tyrosine residues of the VL domain Binding of imidazoles to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10 The second part of our results concerns the binding of imidazole derivatives ... The binding of the ®rst CN± ligand to the iron was thus more easy in the hydrophobic binding pocket of the antibody than the binding of the second one, most probably because of the steric hindrance...
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báo cáo hóa học: " The effect of hip abduction on the EMG activity of vastus medialis obliquus, vastus lateralis longus and vastus lateralis obliquus in healthy subjects" pot
... during nine sets of different exercises obliquus1– VLO) right lateralshowing the origin lateralis uus portion of theinthe superiorintermuscular septum (LIS) Lateral insertion in vastus lateralis ... retinacular tenderness in patients with patellofemoral pain, including some who demonstrated histological changes within the perineural tissues of the lateral retinaculum on examination of surgical ... lateralis muscle (vastus lateralis obliquus – VLO) in the lateral intermuscular septum (LIS) and its insertion in the superior -lateral border of the patella (P) VLL – vastus lateralis longus Bevilaqua-Grossi...
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Investigations on the antiplasmodial activity of ferrocenyl chalcones
... co-workers provided one of the few detailed investigations into the mechanism of action of ferrochloroquine.31 P 13 According to their proposal, the quinoline ring of ferrochloroquine participated in ... ferrochloroquine and ferrocifens are good illustrations that come to mind In keeping with the utilization of ferrocene in drug design, there is a growing interest in understanding how the ferrocene ring contributed ... structures (PLS) The mode of antiplasmodial action of ferrocenyl chalcones was investigated by determining their binding affinity to heme, inhibition of β-hematin formation, inhibition of GSH mediated...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 3
... of hematin The interaction of chalcones with porcine hematin was investigated by monitoring changes in the Soret band of hematin, following a reported method 25 Appropriate aliquots of hematin ... 3-quinolinyl 4-quinolinyl 2,4-dimethoxy 3-quinolinyl 4-quinolinyl 3-quinolinyl 2,4-difluoro 1-naphthalenyl 2-pyridinyl 2-naphthalenyl 4-quinolinyl 4-chloro 1-naphthalenyl 3-quinolinyl 2-pyridinyl ... methemoglobin in the incubation mixture The same volume of DMSO used to deliver test compound was added in both controls 6.2.2 Inhibition of falcipain-2 Experiments on the inhibition of falcipain-2 were...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 2
... difficulties in the experimental determination Capacity factors were not determined at other pH values besides pH A determination at pH may be warranted in view of the hypothesis that the chalcones interfere ... The experimental method involves determining the retention times of a chalcone as it elutes out from a hydrophobic stationary phase using a mobile phase comprising of methanol-water in different ... the plot of predicted against observed activity for the model under consideration d Training set e RMSEP (root mean square error of prediction) for all compounds except those in the training set...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 1
... barriers and organelles Factors that interfere with choline availability to infected cells would understandably undermine normal parasite growth The increased uptake of choline into infected erythrocytes ... Determination of in vitro antimalarial activity 81 5.4.2.2 Determination of in vitro antileishmanial activity 81 5.4.2.3 Determination of physicochemical properties of chalcones by molecular modeling methods ... vacuole, namely interference with the enzymatic activity of a crude plasmodial extract on the breakdown of methemoglobin, inhibition of recombinant falcipain-2, and binding to hematin It appeared...
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Tài liệu The Influence of Human Activity on the Environment ppt
... harmful effects Develop alternative energy sources • Conserve our rapidly diminishing fossil fuel reserves by using solar power or wind power Benefits of conserving endangered species • Ecotourism ... Species indicators • Maintaining diversity in gene pool • WHAT ELSE????????? This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand ... intensive farming of animals to be cruel • In order to produce more food from the land farmers have to use more fertilisers and pesticides Problems with Fertilisers • Fertilisers enable farmers...
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Báo cáo khoa học: Effect of annealing time of an ice crystal on the activity of type III antifreeze protein pdf
... starting the TH measurement at a slower cooling rate We therefore examined whether an annealing period of 2–3 h in uenced the TH value of nfeAFP8 An annealing experiment was performed within the ... control of the freezing plate in our system is stepwise, a different cooling rate will produce a different time lapse of cooling on a sample In other words, there is a longer annealing period before ... mechanism of type III AFP that causes TH enhancement over a very slow time scale Results Before determining the TH activity of nfeAFP8, the performance of our photosystem was verified using solutions of...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... efavirenz interactions we found that a was 3.5 and 3.0, respectively, indicating that the binding of an AQ derivative results in a reduction of efavirenz binding and, vice versa, the binding of efavirenz ... heterodimer with binding areas of alizarine derivatives found after the blind docking experiment The most favorable binding sites are highlighted in blue Thumb Fingers RNAse H Palm Polymerase Fig ... obtain a deeper understanding of the RTligand interactions, QM polarized docking (Schrodinger Inc, Portland, OR, ă USA) was carried out QM polarized docking workow combines docking with ab initio...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... dominant in the presence of 14 lm of heliquinomycin, and larger complexes were detected in the presence of 43 lm of heliquinomycin Thus, it appears that higher concentrations of heliquinomycin ... quantified The average level of intensity in the cells cultured in the presence of heliquinomycin was expressed in comparison with that in the cells cultured in the absence of heliquinomycin FEBS Journal ... polymerase-a/primase are also inhibited at higher concentrations of heliquinomycin may suggest that heliquinomycin interacts with single-stranded DNA to interfere with the activities However, there...
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Báo cáo khoa học: Effect of magnesium ions on the activity of the cytosolic NADH/cytochrome c electron transport system pptx
... was determined in the experiments summarized in Fig The amounts present in pellets of mg of mitochondrial protein, incubated in mL of medium and then centrifuged, were determined to better appreciate ... content of mitochondria incubated in the absence and presence of exogenous cyto-c The determination of endogenous cyto-c was performed in pellets of mg protein of mitochondria incubated for 10 in ... frequently forming and breaking bridges between different points of the inner and outer membranes; over time, the two membranes could be involved in the contact sites, forming these structures in all...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
... proteincarbohydrate interactions are often of moderate afnity and a rather small energy difference between comparable binding events possibly elicits functional differences of AMY1 and AMY2 in ... surface of the catalytic domain or an intimately associated domain rather than on a CBM, e.g a starch-binding domain (SBD) [1,3,4,1922] The role of multivalent binding in enzymatic degradation of ... identity within a subfamily Only one AMY1 and two AMY2 isoforms were found in germinating seeds from a total of 10 barley a-amylase encoding genes; these three proteins moreover underwent differential...
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Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc
... capable of killing 99% of the cells in a suspension containing 106 CFUÆmL)1 In addition, to determine the in uence of the hydrophobic core thickness of the cell membrane on peptide antibiotic activity, ... potential is given in previous reports [33,34] Determination of intracellular pH The intracellular pH of A laidlawii and S melliferum cells was determined by spectrofluorometry using the internally conjugated ... 5(6-)-carboxyfluorescein succinimidyl ester [37] as described previously [33] Protein and cholesterol determination Protein was determined with the bicinchoninic acid method [38] using BSA as standard...
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Báo cáo khoa học: On the mechanisms of bananin activity against severe acute respiratory syndrome coronavirus pptx
... mutations in membrane protein This finding initially suggested that the observed antiviral activity could arise from bananin interference on a hypothetical helicase–membrane protein interaction, as in ... differently filled by bananin because binding with the small molecule involves a larger molecular interface in the case of the former helicase Furthermore, the absence of the S259 OH group in the ... demonstrated that bananin effectively interferes with SARS-CoV ATPase activity by inhibiting helicase activity Furthermore, in a cell culture system of SARS-CoV, bananin inhibited viral replication...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
... C-terminal pair of Ca2+ -binding sites in CaM bound to the IQ-motif directly following the converter domain inhibits Myo1d actin-activated ATPase activity Deletion of two amino acids at the interface ... either abolished, slowed or not affected [18 20] For myosin 1c it has been demonstrated that binding of Ca2+ to the C-terminal pair of Ca2+ -binding sites in CaM inhibits its actin-translocating activity ... converter and IQ-motif led to a Ca2+ -independent inhibition of the actin-activated ATPase activity Results Binding of Ca2+ CaM to the two Myo1d IQ-motifs The light chain binding domain of rat...
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Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
... the solvent deuterium in TFE/water within 25 This indicates that the atom He1 is either involved in a specific interaction, such as hydrogen bonding in SDS micelles, or is buried in a hydrophobic ... nm were intensified with increasing percentages of TFE, which indicates that the helicity of the peptides increased within more hydrophobic environments The spectral change induced by the increased ... cetyltrimethylammonium bromide In doing so, the fluorescence yield drops in the former, while it increases in the latter case However, these interactions commonly result in significant blue shifts in their fluorescence...
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Báo cáo Y học: Effect of coenzymes and thyroid hormones on the dual activities of Xenopus cytosolic thyroid-hormone-binding protein (xCTBP) with aldehyde dehydrogenase activity potx
... retinolbinding protein and cellular retinoic acid-binding protein in the metabolic channeling of retinoids In Retinoids Progress in Research and Clinical Applications (Livrea, M.A & Packer, L., ... constants were determined under initial velocity conditions, which were linear with time and protein Determination of NAD content The content of NAD (the sum of its reduced and oxidized forms) in Xenopus ... described in Materials and methods Nonspecific binding was determined from the samples incubated in the presence of lM unlabeled T3 and subtracted from the total binding The activity of the retinoic...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25