cơ hội cho doanh nghiệp nông nghiệp việt nam thâm nhập thị trường mới nổi
Báo cáo y học: "HIV-1 Tat protein enhances Microtubule polymerization" docx
... microtubule dynamics in living cells and lead to apoptosis after cell cycle disturbance [21-24] They activate the intrinsic mitochondrial apoptotic pathway as shown by the mitochondrial membrane ... mitochondria MDAs induce apoptosis through the mitochondrial apoptosis pathway Our previous work on paclitaxel showed that this agent is able to induce cytochrome c release from purified mitochondria ... mitochondria (lane T-) In parallel, cytochrome c levels decreased slightly in mitochondria treated with Tat, as revealed by Western Tat induces cytochrome c release from isolated Figure mitochondria...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... corresponded to the expected cDNA (here named ACMSD I), while the higher band (1068 bp) corresponded to the cDNA currently in GenBank under accession number AAH16018 (here named ACMSD II) ACMSD II coding ... 2154–2161 Bender DA & Olufunwa R (1988) Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells Br J Nutr 59, 279–287 ... nicotinamide adenine dinucleotide biosynthetic pathways delays axonal degeneration after axotomy J Neuroscience 26, 8484–8491 Cooper BF, Sideraki V, Wilson DK, Dominguez DY, Clark SW, Quiocho FA...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... construct and grown until a D of 0.8 (600 nm) in Terrific Broth supplemented with phosphate buffer and kanamycin at 37 °C The temperature was reduced to 27 °C and the culture induced with 0.5 mm isopropyl-b-d-thiogalactopyranoside ... Immunol 199, 25–38 10 Fabry CM, Rosa-Calatrava M, Conway JF, Zubieta C, Cusack S, Ruigrok RW & Schoehn G (2005) A quasiatomic model of human adenovirus type capsid EMBO J 24, 1645–1654 11 Saban ... surface, independently of endocytosis and escape to the cytosol J Virol 74, 7085– 7095 Zubieta C, Schoehn G, Chroboczek J & Cusack S (2005) The structure of the human adenovirus penton Mol Cell 17,...
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Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx
... 223–226 13 Achouri, Y., Rider, M.H., Schaftingen, E.V & Robbi, M (1997) Cloning, sequencing and expression of rat liver 3-phosphoglycerate dehydrogenase Biochem J 323, 365–370 14 Achouri, Y., ... Williamson, D.H., Lund, P & Krebs, H.A (1967) The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver Biochem J 103, 514–527 47 Guynn, R.W., Merrill, ... Plasmodium, Giardia, Trypanosoma, Toxoplasma, Schistosoma, Theileria, Cryptosporidium, Eimeria, Trichomonas and nonparasitic protozoan Dictyostellium discoideum, but did not find orthologues in these...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... unlabeled 0.25 mM CR I–II was prepared in 50 mM deuterated Tris, 25 mM NaCl pH 8.1 The stimulated-echo with longitudinal eddy current delay (STE-LED) method was applied to obtain diffusion ordered ... as they fall in the crowded part of the spectrum and their assignments could not be transferred unambiguously, even though some of these peaks are still observed in this HSQC A pH titration indicated ... assigned at pH 6.7 The CSI scores at pH 6.7 are added to Fig as this data could not be confirmed unambiguously at pH 7.7 Our secondary structure assignment is consistent with known capping motifs...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... Botting (Eur J Biochem 269) Nishizuka, Y & Hayaishi, O (1963) Studies on the biosynthesis of nicotinamide adenine dinucleotide J Biochem (Tokyo) 238, 3368–3377 Mellilo, G., Musso, T., Sica, A., Taylor, ... potent inhibitor of rat and recombinant human kynureninase Biorg Med Chem Lett 12, 361–363 17 Schott, H.-H & Krause, U (1979) Purification and characterization of 3-hydroxykynureinase from yeast...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... submicromolar range On the other hand, differences between the apo-calhepatin and holocalhepatin hydrodynamic volume (16.8 ± 0.1 kDa and 14.6 ± 0.2 kDa, respectively) suggest that calhepatin undergoes ... increases its intrinsic fluorescence intensity and kmax This result, the decrease in the protein hydrodynamic volume, and the fact that Ca2+-loaded calhepatin is retained on a phenylSuperose column and...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx
... in modified Thermus medium containing 15% glycerol at )70 °C One isolate, designated WR-249, was chosen for this study After the extraction of genomic DNA, PCR-mediated amplification, and sequencing ... Rep, M., van Dijl, J.M., Suda, K., Schatz, G., Grivell, L.A & Suzuki, C.K (1996) Promotion of mitochondrial membrane complex assembly by a proteolytically inactive yeast Lon Science 274, 103–106...
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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
... lgÆmL)1 ampicillin and 25 lgÆmL)1 kanamycin A mL overnight culture of transformed E coli cells was transferred to 500 mL fresh LB medium containing ampicillin and kanamycin and grown further at 37 ... shikimate pathway and synthesis of GPI anchors [4] The parasite, however, is equipped with the complete battery of TCA cycle enzymes, although the operation of a mitochondrial TCA cycle and its role ... (h/solid line); porcine heart MDH (mitochondrial) (s/solid line); porcine heart MDH (cytosolic) (n/solid line) Enzyme Oxamate Gossypol Pf MDH Porcine mitochondrial MDH Porcine cytosolic MDH P...
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Báo cáo khoa học: Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot
... clone (accession number AM042695); according to the expected function of the encoded protein, we named the corresponding gene Ag-hkt Sequence analysis revealed that the Ag-hkt cDNA clone contains ... 319–328 Kotanen S, Huybrechts J, Cerstiaens A, Zoltan K, Daloze D, Baggerman G, Forgo P, De Loof A & Schoofs L (2003) Identification of tryptophan and beta-carboline as paralysins in larvae of the yellow ... Lesage A, Drijfhout JW, Verhaert P, Dillen L, Claeys M, De Meulemeester H, Nuttin B, De Loof A & Schoofs L (1998) Purification of toxic compounds from larvae of the gray fleshfly: the identification...
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Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf
... N-amidino-2-hydroxypyrrolidine in the enzyme:inhibitor complexes were obtained after 10 ps molecular dynamics Energy minimization and molecular dynamics were performed on a Silicon Graphics O2 workstation (SGI, Irvine, CA, ... 10)4 M MgCl2, and 1.0 · 10)4 M ascorbic acid (pH 7.4) [25] Membrane preparations were free of mitochondria and nuclei as confirmed by subcellular enzymatic marker assays (data not shown) Two-hundred ... d ¼ p.p.m (relative area 2), and two multiplets at about d ¼ p.p.m (relative area each) As the -CHO signal was not observed, the formation of the corresponding free aldehyde (i.e 4guanidobutyraldehyde)...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... donors in the presence of crude cell extracts containing membranes More recently, Kucera and Matchova [26] succeeded in discriminating between the reduction of Fe(III) sulfate by NADH catalyzed ... (Eur J Biochem 271) with the kinetic software package Perrella [31] EZ-FIT developed by Results Choice of a Fe(III) substrate for the enzyme assay Most of Fe(III) reductases have no known native ... oxidoreductases (B) N-Terminal sequences were determined by Edman degradation Multiple alignment of chosen sequences was performed using CLUSTAL W programme Fre, flavin reductases of Pseudomonas syringae...
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Identification and biochemical characterization of tetrahydrolipstatin targets in m bovis BCG at different metabolic states
... weight NaTDC Sodium taurodeoxycholate NRP Non-replicating persistence OD600nm Optical density at 600 nm PBS Phosphate buffer saline Madhu Sudhan Ravindran 18 PC Phosphatidylcholine PDB Protein data ... particles from the serum82 The LDL contains cholesterol, TAGs and phospholipids, and although most of the phospholipids and TAGs are metabolized, the cholesterol is retained by the macrophage mainly ... cholesterol esters and retinyl esters The HSL family includes both mammalian and several bacterial carboxylester hydrolases142-144 Based on biochemical studies, HSL could be considered as a cholesteryl...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... using Trichoderma reesei 992 6a Adv BioTech 2008, 6, 32-35 [36] Holt S.M., Hartman P.A A zymogram method to detect endoglucanases from Bacillus subtilis, Myrothecium verrucaria and Trichoderma ... South Dakota School of Mines and Technology, Rapid City, SD, USA She completed her M.S in Microbiology from North Maharashtra University, Jalgaon, India She worked as research scholar at Agharkar ... Microbiol Biotechnol 2009, 36, 961-970 [25] Rastogi G., Muppidi G.L., Gurram R.N., Adhikari A., Bischoff K.M., Hughes S.R., Apel W.A., Bang S.S., Dixon D.J., Sani R.K Isolation and characterization...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... added at A600 = 3.0 and when the induction was prolonged for 16 h Therefore, these conditions were chosen for large-scale production and approximately 10 g of wet cell paste was obtained from L of ... conformational changes can occur in the protein structure around this temperature To study the thermodynamic stability of SsCU-NH, we measured the residual activity after 10 of incubation at increasing ... (data not shown) Among the ten models obtained using the two versions of the program modeller, we chose the best one both in terms of stereochemical parameters (91.1% of the amino acids in the most...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... DJ & Thorneley RN (1995) Escherichia coli chorismate synthase catalyzes the conversion of (6S) -6-fluoro-5-enolpyruvylshikimate3-phosphate to 6-fluorochorismate J Biol Chem 270, 22811–22815 15 ... compound 6(S)-fluoroshikimate, produced by AstraZeneca Inc (London, UK), is converted to 6-fluorochorismate by the subsequent enzymes in the shikimate pathway, thus 6(S)-fluoroshikimate could block ... target should have no homolog in mammalian cells In bacteria, erythrose 4-phosphate is converted to chorismate through seven steps in the shikimate pathway, which is essential for the synthesis of...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... (S)-HMG-CoA [12] Another enoyl-CoA hydratase, mitochondrial crotonase, is not active using HMG-CoA and measuring the reverse (dehydration) reaction [13] Mitochondrial trifunctional protein (MTP) is the ... in association with variable phenotypes ranging from apparently normal development to severe psychomotor retardation with progressive neurological symptoms [4] At present, three additional forms ... unknown Table Enzymes, genes, and associated diseases of the human leucine degradation pathway Enzyme name EC Gene OMIM Branched chain amino transferase Branched chain keto acid dehydrogenase E1, alpha...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... coli strain M15-pQEidi2 was grown in Luria-Bertani broth containing ampicillin (180 mgÆL)1) and kanamycin (50 mgÆL)1) Cultures were incubated at 37 °C with shaking At an optical density of 0.7 (600 ... spectrometer from Bruker Instruments, Karlsruhe, Germany Analytical ultracentrifugation Hydrodynamic studies were performed with an analytical ultracentrifuge Optima XL-I (Beckman Instruments, ... mass of 38 463 Da in good agreement with the calculated mass of 38 455 Da (data not shown) Hydrodynamic studies on Idi-2 protein of B subtilis Mass spectra were recorded with a Biflex III MALDI-TOF...
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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... putative protein with a molecular weight of 99 kDa and a 373-bp 3¢-untranslated region The gene, named Lox1:Ca:1, contains nine exons and, as already observed for other lipoxygenase genes, a very ... of potato tuber development Plant Cell 13, 613–626 Bell, E., Creelman, R.A & Mullet, E (1995) A choloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... the hydrolysis of Ub-K-TAMRA was improved by five-fold with respect to Ub-AMC Under the conditions chosen for the assay, saturation was not reached with Ub-K-peptide-TAMRA Processing of ubiquitin ... Sonia Buri (all from Novartis, Basel, Switzerland) and Markus Schirle, Manfred Raida, Anne-Marie Michon and Sonja Ghidelli (Cellzome AG, Heidelberg, Germany) for technical support, Rita Schmitz (Novartis, ... Meredith M, Orr A, Cross A, Kathoria M & Parkinson J (1997) A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein EMBO...
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