... able to bind tothe ribosome inthe presence of wild-type eEF2 coded forbythe remaining eEF2 gene inthe yeast strain YOR133w (Fig 1) It was therefore possible that these mutants could interfere ... described by Hoffman and Winston [47] The gene for eEF2 was amplified by PCR using primers eEF2F and eEF2R (supplementary Table S1) The 2.5 kb PCR-product was introduced into the TOPO vector pYES2.1 and ... Ca2+ and calmodulindependent protein kinase (CaMPKIII) [15,16] The activity of the eEF2 kinase is regulated bythe mitogen-activated protein (MAP) kinase and mTOR-signalling pathways [17] These...
... Consultants for Requirements and Systems Engineering Requirements arenot just for Christmas BAE Systems Moving the Comfort Zone Data Model Data Model in Action Benefits so far The Future Telelogic User ... Consultants for Requirements and Systems Engineering Requirements arenot just for Christmas BAE Systems Moving the Comfort Zone Data Model Data Model in Action Benefits so far The Future Telelogic User ... Requirements and Systems Engineering Moving the Comfort Zone Culture and existing processes DOORS Perspective Telelogic User Group Conference - 20th November 2007 11 Consultants for Requirements and Systems...
... Consultants for Requirements and Systems Engineering Requirements arenot just for Christmas BAE Systems Moving the Comfort Zone Data Model Data Model in Action Benefits so far The Future Telelogic User ... Consultants for Requirements and Systems Engineering Requirements arenot just for Christmas BAE Systems Moving the Comfort Zone Data Model Data Model in Action Benefits so far The Future Telelogic User ... Requirements and Systems Engineering Moving the Comfort Zone Culture and existing processes DOORS Perspective Telelogic User Group Conference - 20th November 2007 11 Consultants for Requirements and Systems...
... methods should not be used The problem with these estimators is that they focus on when patients die inthe hospital rather than whether they die The quality of a patient’s life inthe ICU is very ... confuse longer survival with better morality The Proportional Hazards model estimated using the methods of Fine and Grey or by using standard software as defined above measures the difference in ... Critical Care Vol 10 No Schoenfeld estimator as the CIF when there are no patients still alive inthe hospital and will approximate it if there are only a few Why ‘survival’ and competing risk...
... useful to see if there were any alert flags set bythe annotator, and, forthe more inquisitive reader, to see what data quality is reported inthe experimental section In addition to providing the ... between the true andthe currently modeled structures In such a map, the parts existing inthe structure, but not included inthe model, should show up inthe positive map contours, whereas the parts ... is not reused Any changes to atomic coordinates result in a new accession code; the old files are then moved into the ‘obsolete area’, but can still be accessed (with some effort) Structural information...
... adenosine modulates the production of inflammatory cytokines, including TNFα, IL-10, and IL-12 [23,25-27,47] In addition tothe regulatory effect of adenosine in cytokine secretion, we have further ... responsible forthe deamination of adenosine to inosine) in animal models of acute and chronic inflammation [8] Moreover, adenosine receptor antagonists and deletion of adenosine receptors eliminates the ... similar roles in regulating inflammation inthe peritoneum We examined the pharmacologic mechanism by which methotrexate diminishes inflammation inthe thioglycollate-induced peritoneal inflammation...
... score for both the hands and feet Seventeen of these patients were judged to show repair bythe experts viewing only the joint-of-interest in Exercise I; inthe remaining six cases, the two independent ... experiment The images were again randomized and recoded so they were not presented inthe same sequence as forthe first exercise, andthe readers were not informed as to which joints had been ... judgement in Exercise I The joint-of-interest inthe first exercise was then indicated tothe readers, and they repeated their review and chose which joint was better, and which film was first in time,...
... design, interpretation of the data and drafting of the manuscript ND contributed to acquisition of the data and drafting the manuscript BD helped designing the study and drafting the manuscript and ... markers of response to therapy and thus be related to outcome Therefore, we investigated whether baseline status or levels of ACPA or IgM-RF and their changes inthe year thereafter are associated ... between changes in antibody levels and changes in disease activity inthe same time period [2] We arenot aware of any study reporting changes in levels as a predictor for outcome later inthe disease...
... aspartate aminotransferases; inthe heart, creatine kinase and troponin T), which are released into the serum during ongoing necrosis These markers are widely used in clinical practice However, the results ... out the ELISA measurements inthe laboratory, drafted and wrote the manuscript, and prepared the tables and figures CB participated inthe design of the study and has been involved in revising the ... fragments containing the CK-18 Asp 396 neoepitope without detecting native or intact CK-18 [24,27] for assessing apoptosis, in combination with measuring total CK-18 as an indirect marker for necrosis...
... protein folding and quality control factors for entry into host cells Cell 131, 516– 529 Sandvig K & van Deurs B (2002) Transport of protein toxins into cells: pathways used by ricin, cholera toxin ... delivered tothe ER [43] Thus, endocytosed StxB was delivered into the endocytic recycling pathway within min, was transferred to perinuclear endosomes within 10–20 min, and then was delivered tothe ... circuitous path from plasma membrane to cytosol Other toxins such as diphtheria toxin and anthrax toxin take advantage of low endosomal pH to penetrate the endosomal membrane and enter the cytosol...
... Furthermore, the UV spectra of the materials in peaks II and IV were in agreement with the UV spectra for eoxins (Fig 4, inset) [11] To further analyze the identity of the materials in peak II and ... (40 lm) forthe indicated time The incubations were terminated by adding one volume of methanol Samples were centrifuged (1400 g for min) andthe supernatants were diluted with water to a maximum ... was set to 21 for MS ⁄ MS at 626 m ⁄ z andto 18 for MS ⁄ MS at both 497 and 440 m ⁄ z The metabolite spectra were compared with those of synthetic standards Immunostaining Formalin-fixed and paraffin...
... change the appearance of the Terminal window by selecting the Window Settings option from the Terminal menuThe Terminal Inspector panel appears, allowing you to configure the settings forthe current ... window manually by clicking the red Close button inthe upper left-hand corner of the Terminal window You can change this behavior inthe Window settings for Terminal by setting the windows to ... After theoptionsinthe command line, you can add arguments inthe form of filenames or text The shell command passes these arguments along tothe command that you are executing, and they give the...
... rather than their actions as a result of it.”6 Will mass advertising diminish in its in uence and use? I think so People are increasingly cynical about and increasingly inattentive to advertising ... services, and programs to serve these chosen markets, and calls upon everyone inthe organization to think and serve the customer.” In short, marketing’s job is to convert people’s changing needs into ... Marketing, Wharton School of Business “Here is anything and everything you need to know about where marketing stands today and where it’s going tomorrow You can plunge into this tour de force...
... rather than their actions as a result of it.”6 Will mass advertising diminish in its in uence and use? I think so People are increasingly cynical about and increasingly inattentive to advertising ... services, and programs to serve these chosen markets, and calls upon everyone inthe organization to think and serve the customer.” In short, marketing’s job is to convert people’s changing needs into ... Marketing, Wharton School of Business “Here is anything and everything you need to know about where marketing stands today and where it’s going tomorrow You can plunge into this tour de force...
... change the appearance of the Terminal window by selecting the Window Settings option from the Terminal menuThe Terminal Inspector panel appears, allowing you to configure the settings forthe current ... window manually by clicking the red Close button inthe upper left-hand corner of the Terminal window You can change this behavior inthe Window settings for Terminal by setting the windows to ... After theoptionsinthe command line, you can add arguments inthe form of filenames or text The shell command passes these arguments along tothe command that you are executing, and they give the...
... g, for users inthe file group, o, for others — users notinthe group — or a, for all users You can designate more than one set of users by combining these designators together, such as go, to ... directory and add it tothe directory stack, usethe command pushd pathname This command adds a new pathname tothe stack, and prints the stack You can usethe directory stack by typing popd to ... DIRECTORIES There is another way to remove an entire directory and its contents, hidden or otherwise, using the rm command The -r option instructs the rm command to remove a directory and everything...
... automatically uses the full Terminal window If there are additional lines on the screen that vi does notuseto display the file, a tilde (~) appears at the beginning of each of these lines These arenot ... tothe beginning of the line, you can type d^, because the ^ keystroke moves the cursor tothe beginning of the line To delete the current line, you can type dd If you want to delete everything ... lines of text together onto one line, you can usethe editing command J This removes the line break at the end of the current line, and joins it tothe next line When you edit the text in your file,...
... define an area of the screen to capture by dragging the mouse Typing a space in Interactive mode switches the selection from mouse selection to window selection; if you click a window in Window ... addition, the top command provides useful information about processes in general and system performance The information included inthe top output for each process includes the process ID (PID), the ... command and continues processing The command to display lines of text in awk is print, not echo You must include lines of text in single quotation marks Awk uses { and } markers to begin and...
... by using the chmod command and giving it a name ending in command When you double-click the icon of a command file, the Terminal program opens a new window and executes the command in that window ... possible for people in New Jersey to send e-mail to people in Ethiopia, for teens in California to read their Pennsylvania grandmother's recipes on the Web, andfor people to chat and form meaningful ... not need the password Entering su - invokes the environment of the new user in addition to assuming their username This command leaves you positioned inthe home directory of the user, with the...
... system does notuse these files; it uses NetInfo Therefore, learning to browse the NetInfo information base and make changes is essential for understanding and monitoring your system The most important ... command option saves you the time of canceling jobs individually The Print Center is a useful tool for monitoring and controlling your printer; it allows you to: determine the status of a printer; ... Unix to store information about systems, users, and so on The early Unix systems stored all user information inthe /etc/passwd file, and information about the local system and other systems in...