brain metastases gene amplification using quantitative real time polymerase chain reaction analysis
Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... http://www.virologyj.com/content/6/1/142 Figure Establishment of the fluorescent quantitative real- time PCR (FQ-PCR) standard curve Establishment of the fluorescent quantitative real- time PCR (FQ-PCR) standard curve Ten-fold dilutions ... the fluorescent quantitative real- time PCR (FQ-PCR), has been used widely to quantify the number of genomic copies of pathogenic microorganisms [18,19] GPV detection by real- time PCR has only ... The FQ-PCR amplification curves and the corresponding FQ-PCR standard curve (Figure 1) were generated by employing the successively diluted known copy numbers of pVP3 for real- time PCR reaction...
- 7
- 338
- 0
Báo cáo y học: "Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit" pdf
... using conventional genetic technology A new assay, termed SeptiFast (Roche Diagnostics, Mannheim, Germany), enables rapid, multiplex testing for micro-organisms using a real- time polymerase chain ... neutropenia using 16 S rRNA Yanagihara et al Critical Care 2010, 14:R159 http://ccforum.com/content/14/4/R159 14 15 16 17 18 19 20 Page of gene amplification by polymerase chain reaction Eur ... Yanagihara et al.: Evaluation of pathogen detection from clinical samples by real- time polymerase chain reaction using a sepsis pathogen DNA detection kit Critical Care 2010 14:R159 Submit your...
- 9
- 307
- 0
Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... transplantation by real- time PCR of Page of which two were positive by pp65 antigenemia assay and 12 were negative by both real- time PCR and pp65 antigenemia assay One donor was positive only by realtime PCR ... doi:10.1186/1743-422X-7-322 Cite this article as: Madhavan et al.: “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus ... Among the 26, 15 patients were tested positive for both pp65 antigenemia and real - time PCR The highest copy number detected by real - time PCR was 29, 768 copies/ml and lowest was copies/ml The...
- 7
- 399
- 0
Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection" pptx
... novel real- time reverse-transcriptase polymerase chain reaction J Infect Dis 2004, 189:652-657 15 van Doornum GJ, Guldemeester J, Osterhaus AD, Niesters HG: Diagnosing herpesvirus infections by real- time ... included in the subsequent analysis Table Viruses identified by conventional methods and real- time PCR Pathogen Viral culture (n = 21) Immunofluorescence (n = 22) Real- time PCR (n = 23) RSV A/B ... needed to determine the real clinical impact of multiple infections and to determine whether the use of real- time PCR prevents unnecessary antibiotic treatment However, real- time PCR offers a rapid,...
- 7
- 539
- 0
Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... microarray analysis) YD: Experiments (cell culture and quantitative real- time PCR analysis) YF: Experiments (cell culture and quantitative real- time PCR analysis) MI: Experiments (analysis with ... interaction Figure Gene networks and pathways of 11 genes from PCA analysis The network was analyzed using Ingenuity Pathways Analysis software and is displayed graphically as nodes (genes /gene products) ... pulmonary adenocarcinoma by expression profiling of eight genes as determined by quantitative real- time reverse transcriptase polymerase chain reaction J Clin Oncol 2004, 22:811-819 Fan X, Shi L,...
- 12
- 520
- 0
Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... to 1:107 CEA mRNA Analysis by Real- Time Quantitative PCR Quantitative PCR was performed using the Sequence Detector System, ABI PRISM 7500 (Applied Biosystems 7500 Fast Real- Time PCR System) PCR ... Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real- time reverse transcriptase -polymerase chain reaction to predict recurrence of gastric adenocarcinoma Journal ... tumour cells in patients with non-small cell lung cancer by real- time fluorescent quantitative reverse transcriptase -polymerase chain reaction in peroperative period J Cancer Res Clin Oncol 2006,...
- 8
- 439
- 0
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference Obviously there is no "one good gene ... cells were harvested at several time points (table 1) and RNA was extracted The RNA transcription level of putative reference genes was determined by quantitative real- time PCR as described below ... reference gene selection for quantitative realtime PCR Biochem Biophys Res Commun 2004, 313:856-862 Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP: Determination of stable housekeeping genes, differentially...
- 5
- 452
- 0
báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference Obviously there is no "one good gene ... cells were harvested at several time points (table 1) and RNA was extracted The RNA transcription level of putative reference genes was determined by quantitative real- time PCR as described below ... reference gene selection for quantitative realtime PCR Biochem Biophys Res Commun 2004, 313:856-862 Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP: Determination of stable housekeeping genes, differentially...
- 5
- 539
- 0
A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... was done using MPB64 primers (Sigma Aldrich/ Bangalore Genei) which are specific for Mycobacteria of the tuberculosis complex 16 Amplification reaction was typically performed in a 50µl reaction ... scale use of polymerase chain reaction in a routine mycobacteriology laboratory J Clin Microbiol 1993; 31: 2049-56 Noordhoek GT, Van Embden JD, and Kolk AH Reliability of nucleic acid amplification ... Broor S, Chakraborty M, Pande JN, Seth P Diagnosis of pulmonary tuberculosis by polymerase chain reaction for MPB64 gene: an evaluation in a blind study Indian J Chest Dis Allied Sci 1998 Jan-Mar;...
- 8
- 524
- 0
Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... of time It is not suitable for large-scale applications In recent times, a more sensitive, time- saving and advanced method has emerged in the field, which is fluorescent quantitative real- time ... enteritis virus by polymerase chain reaction Avian Dis 1998, 42:554-564 31 Hansen WR, Brown SE, Nashold SW, Knudson DL: Identification of duck plague virus by polymerase chain reaction Avian Dis ... 35 Yang F, Jia W, Yue H, Luo W, Chen X, Xie Y, Zen W, Yang W: Development of quantitative real- time polymerase chain reaction for duck enteritis virus DNA Avian Dis 2005, 49:397-400 36 Guo Y, Cheng...
- 10
- 293
- 0
Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... cloned gene fragments were sequenced using an ABI Prism 3100 Genetic Analyzer (Applied Biosystems) Standard curves were generated for each gene to evaluate primer efficiency and for data analysis ... amplified by PCR using primers specific for Vb, Cb, and G3PDH genes Standard curves were generated using the purified PCR products Representative results from a Cb primer-based reaction are shown ... McGuinness PH, Richards C, Putten KV, McCaughan GW, Eris JM, Bishop GA: Real- time reverse transcriptasepolymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression...
- 9
- 568
- 0
Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... immune function J Virol 2003, 77(8):4950-4959 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real- time quantitative PCR and the 2(-Delta Delta C(T)) Method Methods 2001, ... Radonic A, Thulke S, Bae HG, Muller MA, Siegert W, Nitsche A: Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, ... housekeeping genes would be most reliable for use as normalisers in QPCR we subjected the data to analysis using the GeNorm tool [13] (Table 2) Using the results from the GeNorm analysis it was...
- 5
- 481
- 0
Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... immune function J Virol 2003, 77(8):4950-4959 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real- time quantitative PCR and the 2(-Delta Delta C(T)) Method Methods 2001, ... Radonic A, Thulke S, Bae HG, Muller MA, Siegert W, Nitsche A: Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, ... housekeeping genes would be most reliable for use as normalisers in QPCR we subjected the data to analysis using the GeNorm tool [13] (Table 2) Using the results from the GeNorm analysis it was...
- 5
- 574
- 0
Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... SD, standard deviation Table Sequences of the gene- specific oligonucleotide primers and probes for real- time reverse transcriptase polymerase chain reaction Primer and probe HAS-1 Sequence Position ... 42:476-485 35 Heid CA, Stevens J, Williams PM: Real time quantitative PCR Genome Res 1996, 6:986-994 36 Gibson UEM, Heid CA, Williams A: Novel method for real time quantitative RT-PCR Genome Res 1996, ... real- time reverse transcriptase -polymerase chain reaction for equine infectious anemia virus J Virol Methods 2002, 105:171 39 Yoshida M, Funasaki H, Saito M, Kajitani K, Fujii K: Pathologic gene...
- 7
- 466
- 0
báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... electrophoresis Real- time PCR conditions and analysis PCR reactions were performed in 96-well plates with the Chromo4 Real- Time PCR Detector System (BioRadđ) using SYBRđ Green to detect dsDNA synthesis Reactions ... Microsoft Excel file (Microsoft, Redmond, WA) for further gene expression stability analysis Analysis of gene expression stability For analysis of gene expression stability and rank, geNorm v 3.4 software ... control reference genes using geNorm Average expression stability values (M) of the control reference genes using geNorm Plotted from the least stable to the most stable A: including all genes and primer...
- 10
- 267
- 0
báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... dNTP, 0.4 μL of each primer and 1.25 U enzyme Real- time PCR Mitchell Real- time PCR was performed in an Mx 3005P QPCR system (Stratagene, La Jolla, CA) using a SYBR Green based PCR assay (with ROX ... expression in rice by quantitative real- time PCR Biochem Biophys Res Commun 2006, 345(2): 646-651 Nicot N, Hausman J-F, Hoffmann L, Evers D: Housekeeping gene selection for real- time RT-PCR normalization ... new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics Anal Biochem 2002, 302(1):52-59 doi:10.1186/1471-2229-10-4...
- 11
- 330
- 0
Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
... studies have given evidence for overall safety as well Quantitative real- time PCR (QRTPCR) is the most widely used method for specific quantitative assay of ultra low concentration of pDNA in ... from hsp60 gene, generating 161 bp specific product 500 ng of genomic DNA (gDNA) template was amplified in duplicate in glass capillaries in a final volume of 20 μl using 2× Real time PCR Syber ... PCN/500 ng gDNA) (Fig 1) Finally, mouse and beef Figure Linearity analysis after QRTPCR amplification Linearity analysis after QRTPCR amplification Dilution series of pDNAX (109 – × 10°copies) was...
- 11
- 333
- 0
Quantitative real time PCR
... information for the publication of quantitative real- time PCR) guidelines [1] represent a major milestone in the transformation of the real- time quantitative polymerase chain reaction (qPCR) from a research ... the polymerase chain reaction Cold Spring Harbor Symp Quant Biol 51:263–273 Bustin SA, Nolan T (2004) Pitfalls of quantitative real- time reversetranscription polymerase chain reaction Biomol ... Real- time RT-PCR normalisation; strategies and considerations Genes Immun 6:279–284 21 Tricarico C, Pinzani P, Bianchi S et al (2002) Quantitative real- time reverse transcription polymerase chain...
- 233
- 730
- 0
real-time systems design and analysis, 4th edition
... edition of Real- Time Systems Design and Analysis, with the descriptive subtitle Tools for the Practitioner Hardware for Real- Time Systems Real- Time Operating Systems Fundamentals of Real- Time Systems ... plant Even without defining the term real- time, ” it is probably understood that those events demand timely or real- time processing Real- Time Systems Design and Analysis: Tools for the Practitioner, ... various forms of the term, such as real time, real- time, and realtime may appear But to computer, software, and systems engineers the preferred form is real- time, and this is the convention that...
- 571
- 528
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25