biochemical identification of a to i rna editing sites by the inosine chemical erasing ice method
Báo cáo hóa học: " Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome" pdf
... strain Harris had only basal relationship with these strains Amino acid identity with prototype strain Harris was around 92% Because no full sequence of Figure fication step of VIDISCA Original ... Germany) Enzymatic digestion of cDNA involved HinP 1I, as in the original protocol, and CviAII instead of MseI in order to optimise the 3'-end of the primer used for amplification later on After ... Rotavirus were negative on the supernatant and on the original patient material The unknown isolate was termed BNI-788st In order to type it, supernatant was subjected to VIDISCA, with an additional...
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Báo cáo y học: " Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus" doc
... GGATCCATGCAATCTACTATGACG GTCGACTCAACATCTATTACACATCA 1042-2124 1083 F1 GGATCCATGCAATCTACTATGACG GTCGACTTACAGCTGCCCTCCCTGGAC 1042-1347 306 F2 GGATCCATGTATGGACAGCCTGTTTAT GTCGACTTAAGCTAATGGTCCAGTAGA ... TGCAGGGATCCATGGCAATTGCTGCAGATAGG CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1411-1575 165 F2-3 TGCAGGGATCCATGAATGACGACCAGTTAGAT GTCGACTTAAGCTAATGGTCCAGTAGA 1531-1731 201 F4 F5 TGCAGGGATCCATGAATGACGACCAGTTAGAT CTTTGGTCGACTTATTCCCCCGGATAATAGAT ... TGCAGGGATCCATGTTAGATGGTGACAATATC CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1543-1575 33 F8 TGCAGGGATCCATGGATGGTGACAATATCTAT CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1546-1575 30 F9 TGCAGGGATCCATGGGTGACAATATCTATTAT...
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A numerical study of a permeable capsule under stokes flows by the immersed interface method
... with an immersed fixed interface The key idea of the IIM is to find coefficients of a new finite difference scheme at irregular grid points by using the information of the field variable and its ... One advantage of this method is that it can handle complex geometries easily The main disadvantage of this method is the lack of ability to handle non-linear equations Therefore, this method cannot ... depend on the hydraulic conductivity of the membrane as also theoretically obtained Further numerical investigations show that the inflation and partial detachment of the initially adhered capsule...
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Báo cáo khoa học: "No Peri- and Postnatal Effects on Calves Born After Transfer of in Vitro Produced Embryos Vitrified by the Open Pulled Straw (OPS) Method" ppsx
... embryonic loss and abortion throughout pregnancy, higher incidence of hydrallantois, deviation in vascularisation of the placenta, prolonged pregnancies, increased birth weight and a higher perinatal ... to April 1999 If obstetrical assistance was required, it was not applied until 21⁄2-3 h after rupture of the fetal membranes (allanto-amnion) Immediately after birth, nasal passages were cleared ... of the calves and the herd veterinarian treated AI and OPS-IVP calves One female AI calf never recovered from pneumonia and diarrhoea and was euthanised at months of age Two of the OPS-IVP calves...
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Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase potx
... pre-existing UMP residue at the 3¢-end of U6 snRNA is a prerequisite for this modification reaction to take place In addition, the finding of intermediates of the transferase reaction (Fig 2A, lanes ... coupled to an oligo(dT)–cellulose matrix via A/ T base-pairing In an initial experiment, a fairly crude protein fraction (hydroxyapatite step) was applied to the column At this level of purification, ... notion that the catalytic activity of the U6-TUTase, together with its two specificities outlined above, is associated with a single polypeptide chain Therefore, binding of the U6-TUTase to its...
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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot
... size, which is not further reducible Biochemical analysis of viridis zb63 thylakoids In order to characterize the composition of this minimal PSII identified in viridis zb63, thylakoids isolated ... carotenoids from the b-branch of the biosynthetic pathway (VAZ, violaxanthin, antheraxanthin and zeaxanthin) increased with illumination intensity Thus, carotenoid composition in the mutant is ... modification in antenna proteins In addition, EM of grana particles purified from viridis zb63 plants grown under different light conditions show that the organization in PSII arrays is maintained...
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Báo cáo sinh học: " Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene" potx
... GGGACAAGTG AAAATGTCTC TTCAGGGGAT TCAGCTTAGT GACTTGTCCT ATAAGCATGC AATCCTTAAA GAATCACAGT ACACAATCAA GGGACAAGTC AAAATGTCTC TTGAAAGTAT TAGACTCAGT GACTTGGAGT ACAAACATGC AATTCTTGAA GACTCTCAGT ATACAATTAG ... CTGAAGTAGG AATGCAGTAC GTGAGCACCA CACCGACTAT GAGCCTGCAG CAGAGGTAGG CATGCAGTAC ATTAGTACTG CACTGGGAGC AGAGCGTACA CAGCAGATAC TAAAGAACTC CTCTAGGAGC TGATAGAACT CAACAAATAC TGAAAAATTC 270 270 aMPV/C/US/Co aMPV /A/ UK/3b ... GCAATAAGAT AAATTTCTCA TGCACGGACA TTGAGAGAGA ACAACAAGAT CAACCTAGCG _ CATAA-ATGA -GGAAGGCCA GAATGATTAT GAGTAATTAA GGGGAGATGA TGAGAGATCA TCCAAATT-T GAGTAATTAA 1164 1154 aMPV/C/US/Co aMPV /A/ UK/3b...
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báo cáo hóa học:" Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene" potx
... GGGACAAGTG AAAATGTCTC TTCAGGGGAT TCAGCTTAGT GACTTGTCCT ATAAGCATGC AATCCTTAAA GAATCACAGT ACACAATCAA GGGACAAGTC AAAATGTCTC TTGAAAGTAT TAGACTCAGT GACTTGGAGT ACAAACATGC AATTCTTGAA GACTCTCAGT ATACAATTAG ... CTGAAGTAGG AATGCAGTAC GTGAGCACCA CACCGACTAT GAGCCTGCAG CAGAGGTAGG CATGCAGTAC ATTAGTACTG CACTGGGAGC AGAGCGTACA CAGCAGATAC TAAAGAACTC CTCTAGGAGC TGATAGAACT CAACAAATAC TGAAAAATTC 270 270 aMPV/C/US/Co aMPV /A/ UK/3b ... GCAATAAGAT AAATTTCTCA TGCACGGACA TTGAGAGAGA ACAACAAGAT CAACCTAGCG _ CATAA-ATGA -GGAAGGCCA GAATGATTAT GAGTAATTAA GGGGAGATGA TGAGAGATCA TCCAAATT-T GAGTAATTAA 1164 1154 aMPV/C/US/Co aMPV /A/ UK/3b...
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báo cáo khoa học: " Identification of a GCC transcription factor responding to fruit colour change events in citrus through the transcriptomic analyses of two mutants" potx
... I E D Q gggaagcaggtgaagatgatgttagagaagcaattaaaatcaaaccagaaataatttgag G K Q V K M M L E K Q L K S N Q K ctttacgattataattatgtcgacagagatggtgttagaaaaggattaattgtagtttat 781 tgacaacataatcacaagaaaaacaaaaatgattgtagtaataatttaatttttttcttt ... tgacaacataatcacaagaaaaacaaaaatgattgtagtaataatttaatttttttcttt 841 ccccaacaaaacctcaatgatacaaaagaattttaataaaaaaaaaaaaaaaaaaaaaaa 61 121 181 241 301 361 421 481 541 601 661 Figure Full-length cDNA and deduced protein of ... E K T V C I N D I P Q ctttacatgaaaatgagcatgcaaataagagaggcacttcaattgcagctagaactcgag L Y M K M S M Q I R E A L Q L Q L E L E aagcatcttcatgatcaattagagatgcaaatgaatttacaaaagctgattgaggatcaa K H L H...
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Báo cáo khoa học: "Identification of a novel picornavirus related to cosaviruses in a child with acute diarrhea" pps
... Picornaviridae genus in South Asian children Proc Natl Acad Sci USA 2008 Yamashita T, Kobayashi S, Sakae K, Nakata S, Chiba S, Ishihara Y, Isomura S: Isolation of cytopathic small round viruses with ... in the 5' direction Analysis of the contig sequence showed that this virus has a genomic organization similar to other picornaviruses Figure Genomic organization of Cosavirus Genomic organization ... that had only 55% identity at the amino acid level to its top hit, the VP3 protein of Theiler's-like virus, a murine picornavirus in the genus cardiovirus Picornaviruses are non-enveloped viruses...
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the use of body language in inaugural addresses made by the us presidents = việc sử dụng ngôn ngữ cơ thể trong các bài phát biểu nhậm chức của các tổng thống mỹ
... and worshipping the ancestors; the American kids can learn the traditional values by themselves to individualism and freedom in social activities Secondly, by the main tools o shared identities, ... Significance of nonverbal communication in social activities Although to date, there have been a lot of information available for the significance of nonverbal communication in conducting social ... languages can help individuals in the society to build linkages to the others Each individual should have their own perception of the significance of contribution and donation to the values of...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... Nco1 sites using a forward oligomer 5¢-CTTTATTTTCAGGGCGCCATGAAGCGCG CAAGACCGTCTGAA-3¢ and a reverse oligomer 5¢-AGCT CGAATTCG GATCCGGTACCTCAGAAGGTAGACAG CAGAACC-3¢ For derivitization with TMR, a ... from the virus [19] These data raise the possibility of coupling of fiber release to binding of the RGD motif of the hypervariable loop to cellular integrins Alterations in hypervariable loop topology, ... blue and the fiber peptide in magenta containing the RGD motif, is located at the top of the distal domain within the first insertion, and faces the solvent-exposed exterior of the particle As in the...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... depressing dimerization A rapid degradation of these helices in formic acid which may be caused by their destabilization support this hypothesis Thus, the physical stability of a- PEC is strongly ... for the degradation (see Results) The analyses of the peptides revealed various splitting positions of the amino acid chain This variability cannot be explained by specific acid- or protease-catalyzed ... hydrolysis of a- PEC which is facilitated by a partial unfolding of the two N-terminal helices (Fig 4) The resulting high flexibility of this peptide region may be responsible for destabilization of favored...
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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc
... a fraction of their initial AMA-binding capability Western blotting and ligand-binding assay of HNE-modified OBP Titration of HNE covalent adducts in OBP The immunoblot analysis reported in Fig ... HNE was bound entirely within the barrel of OBP The immediate and quantitative displacement of HNE by undecanal indicates that, as expected in the case of binding equilibria, the formation of reversible ... for the 16-h incubations, in which at least 50% of residual ligand-binding capacity was maintained at the highest [HNE] ⁄ [OBP] values In the case of 20-min incubations, HNE inactivation was even...
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Báo cáo khoa học: RNA reprogramming of a-mannosidase mRNA sequences in vitro by myxomycete group IC1 and IE ribozymes pptx
... GCCCGATGCCGACAGCA GCCCGATGCCGACAGCAGAATGGTTTCACGAACAAGACGTTTGGCAAAACCCTTTATACCAGCCTCCCTTGGGCA GCCCGATGCCGACAGCAGAATGGTTTCACGAACAAGACGTTTGGCAAAACCGAGTACTCCAAAACTAATCAATAT GGGAATTAATACGACTCACTATAGGNNNNNAAAAGTTATCAGGCATGCACCT ... GGGAATTAATACGACTCACTATAGGNNNNNAAAAGTTATCAGGCATGCACCT GGGAATTAATACGACTCACTATAGGNNNNNGATAGTCAGCATGTACGCTGGC GGGAATTAATACGACTCACTATAGGNNNNTAAAAGCAACTAGAAATAGCGT GGGAATTAATACGACTCACTATAGGNNNNAGGGGACCTTGCAAGTCCCCTA GCCCGATGCCGACAGCAGAATGGTTTCACGAACAAGACGTTTGGCAAAACCGGTATGCGCTTAGCCTTAGAC ... GCCCGATGCCGACAGCAGAATGGTTTCACGAACAAGACGTTTGGCAAAACCGGTATGCGCTTAGCCTTAGAC GCCCGATGCCGACAGCAGAATGGTTTCACGAACAAGACGTTTGGCAAAACCCTTTGTACCGACCTCCGCCAA CAGCAGAATGGTTTCACG CAGAAGCTCATCCGGCTG AGCATCACGACGCCGTCA...
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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx
... pattern of CMAAT2 mRNA expression was similar to that of CM-AAT1 except that expression peaked at 39 DAP instead of 41 DAP Shalit et al [21] have also demonstrated an increase of AAT activity during ... synthesis of these two esters is achieved through the acetylation of endogenous isoamyl alcohol and phenyl-2-ethanol and is an indicator of the expression of an AAT activity In addition, in agreement ... Tanaka, K., Sone, H., Nagasawa, N & Tamai, Y (1999) Isolation and characterization of the ATF2 gene encoding alcohol acetyltransferase II in the bottom fermenting yeast Saccharomyces pastorianus...
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báo cáo hóa học:" Identification of a public CDR3 motif and a biased utilization of T-cell receptor V beta and J beta chains in HLA-A2/Melan-A-specific T-cell clonotypes of melanoma patients" potx
... supported by grants from ISS-ACC, Ricerca Finalizzata 2007 Fasc N.ACC5/2, Italian Ministry of Health, Ricerca Finalizzata 2007 Fasc.7OAF4, ISS-ACC, Ricerca Finalizzata 2007 Fasc N.ACC2/R2.6 16 Lefranc ... one after vaccination; fX: amino acid not available; gn: natural Melan -A In dark gray: amino acids identical to the consensus sequences; in light gray: other preferentially used amino acids at the ... [64] will be available, and the spatial relationships between Melan -A and CDR3 amino acids will became clearer Conclusion The finding of a conserved amino acid motif in the CDR3, together with the...
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báo cáo hóa học:" Identification of a biomarker panel using a multiplex proximity ligation assay improves accuracy of pancreatic cancer diagnosis" doc
... location of the pancreas, localized deep within the abdominal cavity, and oftentimes nonspecific clinical symptoms such as general abdominal pain, weight loss, and jaundice Chronic pancreatitis, ... patients with PLA profiling of putative pancreatic cancer biomarkers After applying advanced statistical methods to this dataset, we identified a panel of three biomarkers that exceed the diagnostic ... disadvantage of being statistically optimistic The present study also has the advantage of increased size and statistical resolution, considering greater than twice as many cases compared to the...
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Báo cáo hóa học: " Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: Identification of a candidate etiologic agent" pptx
... PCR using Tailed_RNAligateRC, 5'AAGCAGTGGTAACAACGCAGAGTACGGGTGAAAGTGATAAC-3' and the gene specific primer, ABV3RaceInner, 5'-GCAATCCAGGAATAAGCAAGCACAAA-3' (maps to residues 8595-8620 of vRNA) Both ... transcription initiation and termination sites in bornavirus genomes Panel A, alignment of the bornavirus transcription initiation sites (TIS) and nucleotides of flanking sequences Panel B, alignment of ... revision of the manuscript Additional material Additional file Alignment of bornavirus genomes 5' and 3' termini Bornavirus genome organization overview diagrammed as in Figure Sequences in alignments...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... and western blot ananlysis and was assisted in these experiments by JAB and JAAG MM and CR helped in designing appropriate Muc16 primers JC assisted in obtaining and maintaining murine ovarian ... developed murine monoclonal antibodies that were initially generated against human MUC16 List of abbreviations EOC: epithelial ovarian cancer; ConA: Concanavalin A; αMe-Man: α-methylmannopyranoside Competing ... report the expression and initial biochemical characterization of Muc16 expressed by the MOVCAR cells Specifically, we identify expression of Muc16 mRNA and provide evidence that, unlike MUC16, the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008