binding stoichiometry and affinity of fluorescent dyes to proteins in different structural states
Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... protein (B) Comparisons between several ligand binding modes in the catalytic cleft of TLL The stereoview of OA binding modes in molecule A and B (OA_A and OA_B, respectively, thick lines); C9 and ... monomer lying in the catalytic site, in contact with W89, according to two binding modes (see Fig 6) These structural considerations suggest that the FRET was probably due to a molecular binding of ... the lid is involved in the binding of the mixed micelles of cis-PnA/NaTDC On the other hand, these shifts might result from the quenching of the tryptophan fluorescence in the presence of the mixed...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... up to 20 A minimum level of laser power and duration of recording time were set for imaging to minimize photobleaching and cell movement during recording, respectively The average intensity of ... efficient and convenient method for measuring protein– protein interaction The same insect cell line can be routinely used to express any recombinant proteins of interest, allowing various combinations ... sensitive method of visualization of FRET and assessment of protein–protein interactions It can potentially allow for studying the associations of molecules of any proteins of interest Acknowledgements...
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Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... substrate binding and stepping motion in motor proteins, or to probe molecular mechanisms underlying conformational changes We anticipate that this technique will have broad applicability to the ... supplies a control showing that rupture corresponds to strand dissociation, as expected, and not to the breakage of the digoxygeninbased link holding the DNA to the surface Using the methods described ... the outset to permit simultaneous singlemolecule fluorescence and optical trapping The choices of dyes and excitation wavelengths were optimized to minimize two-photon excitation and achieve...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... function of the protein may be related to some critical steps in the conversion of inactive forms of protein to that of active forms It is interesting and intriguing to note that in the absence of ... the latter Interestingly, it appears that protein binding to ssDNA in the absence of any nucleotide cofactor results in attenuation of spontaneous annealing (compare line 1, Fig with line 2, Fig ... thereby showing it has attained its native structure This purified protein showed single and double stranded DNA binding activity Binding to single stranded DNA stimulated a significant level of DNA...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... previously [18,46] that the binding of ligands to the D2 dopamine receptor may exhibit co-operativity, which can be accounted for in terms of interactions between binding sites in an oligomer There ... resuspended in buffer and stored at )80 °C in aliquots of 500 lL The protein concentration was determined by the method of Lowry et al [32], using BSA as the standard Radioligand -binding assay ... EcoRI site at the 3¢ end of the insert, to produce the recombinant plasmids TOPOD2L and TOPOD2S, respectively In order to add an epitope tag to both receptors at their N-terminus, complementary synthetic...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... fluorescent proteins in the following four combinations: cerulean-elongin B and citrineelongin C; cerulean-elongin B and elongin C-citrine; elongin B-cerulean and elongin C-citrine; and elongin B-cerulean ... encoding: (C) cerulean-elongin B and elongin Ccitrine; (D) elongin B-cerulean and elongin C-citrine; (E) ceruleanelongin B and citrine-elongin C; and (F) elongin B-cerulean and citrine-elongin ... cerulean-linked elongin B and citrine-linked elongin C can be detected in the following donor-acceptor combinations in decreasing order: cerulean-elongin B and citrine-elongin C > cerulean-elongin B and...
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Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot
... [20] In AC8D582)594, a region from Y582 to L594 in the C1 domain of AC8 was deleted; in AC8D1126)1248 [20], a region from R1126 to P1248 in the C-terminus of AC8 (in the C2 domain) was deleted; in ... camera (Princeton Instruments), z-step motor and dual ®lter wheels controlled by SLIDEBOOK 3.0 software (Intelligent Imaging Innovation, Denver, CO, USA) Binning ´ mode and 500 ms integration ... although initially appearing to introduce a layer of cumbersome complexity to the structure of adenylate cyclase, may actually be a step in resolving one of Ó FEBS 2002 the more intriguing properties...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... Many kinds of proteins are involved into the DNA compaction process Histone chaperons are a group of proteins in eukaryotic cells to help chromatin assembly The binding mechanisms of histone chaperons ... concentration of the reaction buffer into infinite or to add certain amount of competing DNA, both of which are designed to obtain the optimal ratio of DNA to histone proteins As described in the previous ... induced by the binding of histone proteins to DNA at physiological salt concentration: (a) Time course of the assembly and disassembly process of pure histone proteins and DNA 0.01 mg/ml histone...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... GAPDH and PGK linked to cerulean or citrine were introduced into CHO-K1 cells As shown in Figs 1A and S1A, transiently expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal ... C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) were localized in the cytoplasm PGK–cerulean (chimeric protein with N-terminal PGK and C-terminal cerulean) ... We expressed three pairs of chimeric proteins to determine FRET signals with different sizes of linkers connecting GAPDH or PGK to fluorescent proteins (Fig 3) These proteins were relatively evenly...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... alternate interpretation of the lack of agonist-induced BRET observed in this study is that the translation of agonist binding to the ATDs of the family C GPCR homodimer into G-protein coupling of the ... & Mulvihill, E.R (1993) The ligand -binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins Neuron 11, 41–52 14 Tones, M.A., Bendali, N., Flor, ... investigations into the signal transference from the ATDs to the G-protein coupling areas of the receptor homodimer are clearly needed in order to gain a better understanding of the signal transduction...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... concentration [2,5] Some PLCs contain calcium -binding domains [6], and other Ins(1,4,5)P3-metabolizing enzymes, including inositol polyphosphate 3-kinases and inositol polyphosphate 5-phosphatases, ... the combination of increases and decreases in Ins(1,4,5)P3 and PtdIns(4,5)P2 [16] To address the CCh-induced FRET signal changes in PC12h cells to increased Ins(1,4,5)P3 or to decreased PtdIns(4,5)P2, ... level of receptor- and calcium-induced Ins(1,4,5)P3, the removal of both calcium and CCh reduced the receptor- and calcium-induced Ins(1,4,5)P3 to 31% By contrast, the time-dependent restoration of...
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FUNDAMENTALS of KINEMATICS and DYNAMICS
... link to link and to the frame (at point O) A revolute joint is a pin, and it allows rotation in a plane of one link with respect to another A revolute joint also connects the two links and Link ... and new software tools have started making an impact on how the subject of kinematics and dynamics of machines and mechanisms can be taught I have taught kinematics and dynamics of machines and ... tipping of the dump bed All the previous examples involved only links with two connections to other links Such links are called binary links In the example of Figure 1.6, in addition to binary links,...
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Atomistic modeling of energetics and dynamics of diffusive and frictional phenomena in c60 graphene based systems
... barriers into account, to predict the diffusion coefficient as a function of temperature and hydrogen coverage Our findings provide insights into the understanding of the diffusive and frictional ... ―bottomup‖ approach is crucial to address ever-increasing demands for miniaturization of devices [29, 31, 32] In contrast to the top-down approach, in which the structure is imposed, in the bottom-up ... thin film of CNTs, a single CNT connecting two electrodes, a nanoporous metal-organic network consisting of functional molecules and iron atoms, and the letter ―C‖ obtained by manipulating and...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... using lipofectamine Sixteen hours post-transfection, the cells were washed, trypsinized and split at ratio of in 10, in 100 and in 1000 into selective media containing 100 μg/mL of hygromycin ... of in 10, in 100 and in 1000 into selective cell culture media containing 100 μg/mL of hygromycin B and 300 μg/mlL of G418 The diluted cells were re-seeded onto a new 6-well plate and allowed to ... imprecision of the method were studied by using QC samples consisting of a high, medium and low concentration of the five analytes, containing 1, and 10 nM of icariin, icaritin and desmethylicaritin;...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... genistein and Epimedium prenylflavonoids To study combinatorial effects of flavonoids and estradiol, binary mixtures containing increasing doses of genistein and Epimedium prenylflavonoids in the ... genistein, icaritin or desmethylicaritin (Fig 30) 128 Figure 29: Chromatograms of derivatized icaritin, desmethylicaritin and genistein (internal standard) and corresponding EI-MS spectra in full-scan ... obtained for estradiol 93 Figure 13: Isoform selectivity of PPT, DPN and genistein ERα (―•―) and ERβ (―○―) stable cells were exposed to increasing doses of (A) PPT; (B) DPN and (C) genistein (GEN),...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... sex 201 hormone -binding globulin Hence, it is not likely that icaritin will bind to the sex hormone -binding globulin protein found in serum The late appearance of icaritin in sera of human volunteers ... comparable to that of genistein and kaempferol (Maggiolini et al., 2004; Leung et al., 2004) The presence of serum reduced ERα binding of quercetin in MCF-7 cells, with relative binding affinities ... albumin but not sex hormone -binding globulin, which would influence its bioavailability A large proportion of proteins found in serum are made up of albumin, which binds and transports acidic and...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... dose of 100 mg/kg The in- vivo 221 hydrolysis of glycosides like icariin can lead to the production and absorption of aglycones like icaritin and desmethylicaritin into the blood stream and this ... factor-kappa B ligand and osteoprotegerin In this case, osteoprotegerin can act as a decoy receptor for receptor activator of nuclear factor-kappa B ligand which then neutralizes its function in ... especially in terms of icariin but it had a lesser amount of icaritin - 143.3 mg/g icariin, 0.009 mg/g icaritin and 0.122 mg/g desmethylicaritin The amounts of icariside I and icarside II were determined...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... Flavonoid binding to human serum albumin Biochem Biophys Res Commun 398(3):444-9 Boulton, D.W., Walle, U.K and Walle, T 1999 Fate of the flavonoid quercetin in human cell lines: chemical instability and ... Pharmacokinetics of the citrus flavanone aglycones hesperetin and naringenin after single oral administration in human subjects Eur J Clin Nutr 61(4):472-7 Klinge, C.M 2000 Estrogen receptor interaction ... Ma, D.F., Qin, L.Q., Wang, P.Y and Katoh, R 2008 Soy isoflavone intake increases bone mineral density in the spine of menopausal women: meta-analysis of randomized controlled trials Clin Nutr 27(1):57–64...
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Application of formal and dynamic equivalence to the translation of bị and được into english
... helpful to provide: Input for learners of English in order to minimize their errors in translating bị and into English Input for translators with information on error types and solutions to the ... methods of translating bị and into English Application of formal and dynamic equivalence to the translation of bị and into English is also presented This study is also beneficial to anyone who is interested ... ways of transferring opinions; some of them are not included in any kind of grammatical sentences As a result, the translator has to base on the meanings of bị and được, as well as the context in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008