automatic identification of problematic situations

... form Parallel form Fig. 5. CD of Htelo in the presence of various amounts of RGG3. Titration of Htelo with RGG3 (1.2, 1.0, 0.8, 0.6, 0.4, 0.2, 0.1 and 0 equiv. of RGG3) in 100 m M KCl and 50 ... amounts of RGG3. The CD spectrum of Htelo, a hybrid (3 + 1) form, showed a strong positive band at 290 nm and a nega- tive band at around 235 nm, whereas the addition of 1 ratio excess of RGG3 ... 250 0.8 1 0.6 0.4 0.2 0 0.8 1 Fraction of RNA bound Fraction of DNA bound Fig. 4. Binding affinity of RGG3 to Htelo or rHtelo. The DNA or RNA concentration was fixed at 1 n M, whereas the concentration of RNase-treated...

Ngày tải lên: 15/02/2014, 01:20

... percentage of amino acid coverage in the matched proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new ... spectrum of protein 5, fraction 5 ⁄ fraction 6. Fig. S7. MS spectrum of protein 8, fraction 5 ⁄ fraction 6. Fig. S8. MS spectrum of protein 11, fraction 5 ⁄ fraction 6. Fig. S9. MS spectrum of protein ... patterns of activity [13,23]. It has been suggested that the pres- ence of several well-separated peaks in the case of cell- ulosomes from C. cellulovorans may be partly due to the existence of various...

Ngày tải lên: 18/02/2014, 08:20

... four molecules of 3OH-C12:0, two of C10:0, and one of C12:0 [10]. When compared to this struc- ture, the lipid A of O35ElpxX lacks two decanoic acyl (C10:0) substituents, and that of O35ElpxL lacks ... NaCl ⁄ chloroform (1 : 1 v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase was re-extracted with de-ionized water to remove traces of salts, and ... Analysis of physio- chemical and biological features of both mutants was performed to study the functions of these genes and the structures of their resultant LOSs in vitro and in vivo. Results Identification...

Ngày tải lên: 18/02/2014, 14:20

... in mantle. One inducible and two constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increases after thermal ... recovery of cells after protein damage, by assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these ... protein L10, is a transcription cofactor that inhibits activation of AP-1 transcription factors. QM protein is implicated in the conversion of a broad vari- ety of extracellular signals generated...

Ngày tải lên: 18/02/2014, 16:20

... of plasmin in the amidolytic assay. However, if we use the k p ⁄ K m ratio as a mea- sure of the overall impact of stearate, it should be clas- sified as an inhibitor of plasmin, the potency of ... effect of random outliers. The probability distribution of the parameters estimated using this robust evaluation procedure was essential for the identification of the statistical significance of the ... Light attenuation of oleic acid micelles in the presence of synthetic plasmin substrate. (A) Twenty microlitres of 1 mM Spectrozyme-PL was added to 180 lL of various concentrations of oleic acid...

Ngày tải lên: 18/02/2014, 17:20

... Localization of OMM-64 in the inner ear sacculus by immu- nohistochemistry. (A) Macular region of the sacculus after removal of the otolith. Strongly immunoreactive cells were observed at the periphery of ... of the antibody was confirmed by the detection of no bands in the supernatant SDS–PAGE. Subsequently, 100 lL of saccular extract or water-soluble otolith matrix proteins were bound to 10 l L of ... cut-off). Production of rabbit antiserum raised against the recombinant protein and affinity purification of the antibody were performed by Hokkaido System Science (Hokkaido, Japan). Collection of...

Ngày tải lên: 18/02/2014, 17:20

... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on malt agar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... for the rapid detection of hydrophobins from a large number of small samples, we have investigated the potential of intact-cell MALDI-TOF MS (ICMS). Initial applications of ICMS on fila- mentous ... typical MALDI- TOF mass spectra of four strains of H. koningii, T. long- ibrachiatum, H. virens and T. atroviride. The spectra Fig. 1. Alignment of the six hydrophobin protein sequences of H. jecorina....

Ngày tải lên: 19/02/2014, 02:20

... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in their ... min. After the addition of 0.25 mL of 40% potassium hydroxide solution and 0.25 mL of methanol, the cell suspension was boiled for 5 min. Products were extracted three times with 0.2 mL of hexane and evaporated. ... addition of 0.1 mL each of methanol and water. The products were extracted three times with hexane (0.2 mL). After evapor- ation, the residue was dissolved in 0.02 mL of hexane, and 0.001 mL of hexane...

Ngày tải lên: 19/02/2014, 07:20

... number of genes in U6A and U6A-2 cells, IFN-treated U6A-VV-II cells showed the down-regulation of a substantially larger subset of genes. Identification and characterization of a subset of ISGF3-independent ... 1579 expression profile of cells expressing the VV-II mutant form of STAT2 was more similar to that of U6A cells than that of U6A-2 cells (Level 1). IFN treatment led to the further segregation of the sample ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of Immunology, University of Toronto, ON, Canada 2 Division of Signaling...

Ngày tải lên: 19/02/2014, 07:20

... Rosenfeld RG (1997) Identification of a family of low-affinity insulin-like growth factor binding proteins (IGFBPs): characterization of connective tissue growth factor as a member of the IGFBP superfamily. ... the presence of 10 mm CaCl 2 , as described in Experimental procedures. Of the nine cell lines tested, OVISE, Fig. 1. Analysis of noncleaved and cleaved forms of IGFBP-rP1 in conditioned media of eight ... enzyme of IGFBP-rP1. On the other hand, soluble forms of matriptase, which are released from cell membranes by proteolysis, were detected in the conditioned media of at least 5 cell lines out of...

Ngày tải lên: 19/02/2014, 07:20

... in the absence of azide. Ó FEBS 2004 Bioinformatics and mutagenesis of GH-57 (Eur. J. Biochem. 271) 2869 Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues ... study of GH-57, no detailed alignment of the complete sequences of GH-57 members has yet been accomplished. To date, only p artial or selected sequences have been compa red [17–19]. An alignment of GH-57 ... AL662. The analysis of this sequence revealed that the encoded enzyme i s a member of GH-57 [21,22]. The cloning and expression of apu in Escherichia c oli has led to the production of a C-terminally truncated...

Ngày tải lên: 19/02/2014, 12:20

... sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the arithmetic product of its Ó ... absolute magnitude of a combined autoinfluence path is the product of all the rates divided by the product of all the concentrations of its species. Therewith the c oefficient of o rder p of the characteristic ... the basis of the graphical analyses of the characteristic equation and of t he method we develop here. Inspection of Eqn 12 shows t hat in a ll coefficients a p the term consisting of negative...

Ngày tải lên: 19/02/2014, 16:20

... interaction of Yef1p with Utr1p is of biological interest and may be related to the pro- nounced requirement of both Yef1p and Utr1p in the absence of Pos5p. The elucidation of the localization of Utr1p ... The loss of YCp-UTR1 was con- firmed by the Ura– phenotype of MK1219 (require- Table 2. Effect of compounds on ATP-NAD kinase activity of Yef1p. The effect of compounds on the activity of Yef1p ... abilities of LEU2, UTR1 and YEF1 for the growth defects of the pos5 mutant To confirm the complementing ability of LEU2 for the growth defects of the pos5 mutant, we examined the growth of pos5...

Ngày tải lên: 20/02/2014, 01:20

... effect of chloride ions on enzyme activity was investigated (Fig. 4). The hydrolysis of Mca- Fig. 3. Role of His505 and His345 in catalysis. Schematic of the proposed reaction intermediate of ACE2, ... study has highlighted the importance of Arg273 in binding of the C-terminus of peptide substrates by ACE2. The complete loss of activity observed as a result of replacing this single residue could ... nitrogen) of both residues are within hydrogen-bonding distance of the carbonyl oxygen of the amide group of residue P1¢ (tACE) (Fig. 1B) or equivalent terminal carboxylate oxygen (ACE2) (Fig. 1A) of...

Ngày tải lên: 20/02/2014, 01:20

... Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to ... the mechanism of action of our cytotoxic GT oligonucleotides. In this article, we report the identification of eukaryotic elongation factor 1 alpha (eEF1A) as a nuclear component of the CRC in ... the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of...

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Ngày tải lên: 07/03/2014, 00:20