automatic identification of problematic situations
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... formParallel formFig. 5. CD of Htelo in the presence of various amounts of RGG3.Titration of Htelo with RGG3 (1.2, 1.0, 0.8, 0.6, 0.4, 0.2, 0.1 and 0equiv. of RGG3) in 100 mM KCl and 50 ... amounts of RGG3.The CD spectrum of Htelo, a hybrid (3 + 1) form,showed a strong positive band at 290 nm and a nega-tive band at around 235 nm, whereas the addition of 1ratio excess of RGG3 ... 2500.810.60.40.200.81Fraction of RNA boundFraction of DNA boundFig. 4. Binding affinity of RGG3 to Htelo orrHtelo. The DNA or RNA concentration wasfixed at 1 nM, whereas the concentration of RNase-treated...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
... percentage of amino acid coverage in the matched proteins; Mpep, the number of unique matched peptides; Upep, the number of unmatched peptides in the MALDI-TOF experiments. The function of new ... spectrum of protein 5, fraction 5 ⁄ fraction 6.Fig. S7. MS spectrum of protein 8, fraction 5 ⁄ fraction 6.Fig. S8. MS spectrum of protein 11, fraction 5 ⁄ fraction 6.Fig. S9. MS spectrum of protein ... patterns of activity [13,23]. It has been suggested that the pres-ence of several well-separated peaks in the case of cell-ulosomes from C. cellulovorans may be partly due tothe existence of various...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... four molecules of 3OH-C12:0, two of C10:0,and one of C12:0 [10]. When compared to this struc-ture, the lipid A of O35ElpxX lacks two decanoic acyl(C10:0) substituents, and that of O35ElpxL lacks ... NaCl ⁄ chloroform (1 : 1v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase wasre-extracted with de-ionized water to remove traces of salts,and ... Analysis of physio-chemical and biological features of both mutants wasperformed to study the functions of these genes andthe structures of their resultant LOSs in vitro andin vivo.ResultsIdentification...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
... in mantle. Oneinducible and two constitutive isoforms of HSP70 aresynthesized in the gills and mantle of C. gigas [26].The expression level of the constitutive forms increasesafter thermal ... recovery of cells after protein damage, by assisting the folding of cytoskeletal proteins that are actively synthesizedand ⁄ or renatured under these conditions. The upregu-lation of all of these ... protein L10, is a transcription cofactor thatinhibits activation of AP-1 transcription factors. QMprotein is implicated in the conversion of a broad vari-ety of extracellular signals generated...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
... of plasmin in the amidolyticassay. However, if we use the kp⁄ Kmratio as a mea-sure of the overall impact of stearate, it should be clas-sified as an inhibitor of plasmin, the potency of ... effect of random outliers. The probability distribution of theparameters estimated using this robust evaluationprocedure was essential for the identification of thestatistical significance of the ... Light attenuation of oleic acid micelles in the presence of synthetic plasmin substrate. (A) Twenty microlitres of 1 mM Spectrozyme-PLwas added to 180 lL of various concentrations of oleic acid...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... Localization of OMM-64 in the inner ear sacculus by immu-nohistochemistry. (A) Macular region of the sacculus after removal of the otolith. Strongly immunoreactive cells were observed at theperiphery of ... of the antibody was confirmed by the detection of no bands in the supernatant SDS–PAGE. Subsequently,100 lL of saccular extract or water-soluble otolith matrixproteins were bound to 10 l L of ... cut-off). Production of rabbit antiserum raised against the recombinant proteinand affinity purification of the antibody were performed byHokkaido System Science (Hokkaido, Japan).Collection of...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on maltagar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... for the rapid detection of hydrophobinsfrom a large number of small samples, we haveinvestigated the potential of intact-cell MALDI-TOFMS (ICMS). Initial applications of ICMS on fila-mentous ... typical MALDI-TOF mass spectra of four strains of H. koningii, T. long-ibrachiatum, H. virens and T. atroviride. The spectraFig. 1. Alignment of the six hydrophobin protein sequences of H. jecorina....
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... exhibit a wide range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their ... min.After the addition of 0.25 mL of 40% potassium hydroxidesolution and 0.25 mL of methanol, the cell suspension wasboiled for 5 min. Products were extracted three times with0.2 mL of hexane and evaporated. ... addition of 0.1 mL each of methanol and water. The products wereextracted three times with hexane (0.2 mL). After evapor-ation, the residue was dissolved in 0.02 mL of hexane, and0.001 mL of hexane...
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Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
... number of genes in U6Aand U6A-2 cells, IFN-treated U6A-VV-II cells showedthe down-regulation of a substantially larger subset of genes. Identification and characterization of a subset of ISGF3-independent ... 1579expression profile of cells expressing the VV-II mutantform of STAT2 was more similar to that of U6A cellsthan that of U6A-2 cells (Level 1). IFN treatment ledto the further segregation of the sample ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of Immunology, University of Toronto, ON, Canada2 Division of Signaling...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... Rosenfeld RG (1997) Identification of a family of low-affinity insulin-like growth factor binding proteins(IGFBPs): characterization of connective tissue growthfactor as a member of the IGFBP superfamily. ... thepresence of 10 mm CaCl2, as described in Experimentalprocedures. Of the nine cell lines tested, OVISE,Fig. 1. Analysis of noncleaved and cleavedforms of IGFBP-rP1 in conditioned media of eight ... enzyme of IGFBP-rP1. On theother hand, soluble forms of matriptase, which arereleased from cell membranes by proteolysis, weredetected in the conditioned media of at least 5 celllines out of...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
... in the absence of azide.Ó FEBS 2004 Bioinformatics and mutagenesis of GH-57 (Eur. J. Biochem. 271) 2869Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues ... study of GH-57, no detailedalignment of the complete sequences of GH-57 membershas yet been accomplished. To date, only p artial or selectedsequences have been compa red [17–19]. An alignment of GH-57 ... AL662. The analysis of this sequence revealed that the encoded enzyme i s amember of GH-57 [21,22]. The cloning and expression of apuin Escherichia c oli has led to the production of a C-terminallytruncated...
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Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
... sign) of a cycle isthe product of the values (the signs) of its steps. The sign o fthe magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the arithmetic product of itsÓ ... absolute magnitude of acombined autoinfluence path is the product of all the ratesdivided by the product of all the concentrations of itsspecies. Therewith the c oefficient of o rder p of thecharacteristic ... the basis of the graphical analyses of thecharacteristic equation and of t he method we develop here.Inspection of Eqn 12 shows t hat in a ll coefficients aptheterm consisting of negative...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
... interaction of Yef1p with Utr1p is of biological interest and may be related to the pro-nounced requirement of both Yef1p and Utr1p in theabsence of Pos5p.The elucidation of the localization of Utr1p ... The loss of YCp-UTR1 was con-firmed by the Ura– phenotype of MK1219 (require-Table 2. Effect of compounds on ATP-NAD kinase activity of Yef1p. The effect of compounds on the activity of Yef1p ... abilities of LEU2, UTR1 and YEF1for the growth defects of the pos5 mutantTo confirm the complementing ability of LEU2 for thegrowth defects of the pos5 mutant, we examined thegrowth of pos5...
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Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... effect of chloride ions on enzyme activitywas investigated (Fig. 4). The hydrolysis of Mca-Fig. 3. Role of His505 and His345 in catalysis. Schematic of theproposed reaction intermediate of ACE2, ... study has highlighted the importance of Arg273in binding of the C-terminus of peptide substrates byACE2. The complete loss of activity observed as aresult of replacing this single residue could ... nitrogen) of both residues arewithin hydrogen-bonding distance of the carbonyloxygen of the amide group of residue P1¢ (tACE)(Fig. 1B) or equivalent terminal carboxylate oxygen(ACE2) (Fig. 1A) of...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically bindingto ... the mechanism of action of our cytotoxic GT oligonucleotides.In this article, we report the identification of eukaryoticelongation factor 1 alpha (eEF1A) as a nuclear component of the CRC in ... thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding. Anal. Biochem. 72, 248–254.20. Laemmli, U.K. (1970) Cleavage of structural proteins during theassembly of...
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- automatic steering of ships
- classifi cation of theories
- automatic monitoring and control of cooling water treament products
- chinese unknown word identification
- classification of spanish verbs
- automatic classification of verbs
- approach to automatic synonym identification
- classification of feedback expressions
- improve opinion target identification
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