classifi cation of theories
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... form
Parallel form
Fig. 5. CD of Htelo in the presence of various amounts of RGG3.
Titration of Htelo with RGG3 (1.2, 1.0, 0.8, 0.6, 0.4, 0.2, 0.1 and 0
equiv. of RGG3) in 100 m
M KCl and 50 ... amounts of RGG3.
The CD spectrum of Htelo, a hybrid (3 + 1) form,
showed a strong positive band at 290 nm and a nega-
tive band at around 235 nm, whereas the addition of 1
ratio excess of RGG3 ... 250
0.8
1
0.6
0.4
0.2
0
0.8
1
Fraction of RNA bound
Fraction of DNA bound
Fig. 4. Binding affinity of RGG3 to Htelo or
rHtelo. The DNA or RNA concentration was
fixed at 1 n
M, whereas the concentration of
RNase-treated...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
Ngày tải lên :
16/02/2014, 15:20
... Kessler
Department of Clinical Medicine, University of Oxford, UK
Introduction
The genus Yersinia consists of three pathogenic species
that are agents of a variety of diseases, one of which
was historically ... GTPase-binding
capacity of YpkA and OTUB1-mediated increase in
susceptibility to infection (Fig. 6). The binding of small
GTPases has been reported to be independent of the
kinase activity of YpkA [16]. ... can be achieved either by induction
of RhoA mRNA expression, deubiquitination of RhoA
itself or promoting activation of RhoA, possibly
through the manipulation of RhoA-specific guanine
nucleotide...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
Ngày tải lên :
18/02/2014, 08:20
... percentage of amino acid coverage in the matched proteins; M
pep
, the number of unique matched peptides; U
pep
, the number of
unmatched peptides in the MALDI-TOF experiments. The function of new ... maximum peptide mass tolerance
of 150 p.p.m., one missing cleavage allowed, a fixed modifi-
cation of cysteines by iodoacetamide (carbamidomethyl),
a variable modi cation of methionines (oxidation) ... patterns
of activity [13,23]. It has been suggested that the pres-
ence of several well-separated peaks in the case of cell-
ulosomes from C. cellulovorans may be partly due to
the existence of various...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... mean of the fluorescence signal of at least eight wells
containing aliquots of the same solution. The morphology
of Ab aggregates formed from solutions incubated as above
but in the absence of ThT ... two-step puri cation results
in a highly pure product, and yields 10–20 mg of
Ab(M1–40) per liter of culture. In the example shown
in Fig. 3, 30 mg of peptide was obtained from 2.2 L of
bacterial ... broader coverage of struc-
tural assignments to the N-terminus of Ab. Moreover,
the procedures described here are also suitable for
expression and puri cation of mutant versions of
Alzheimer’s...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
Ngày tải lên :
18/02/2014, 14:20
... four molecules of 3OH-C12:0, two of C10:0,
and one of C12:0 [10]. When compared to this struc-
ture, the lipid A of O35ElpxX lacks two decanoic acyl
(C10:0) substituents, and that of O35ElpxL lacks ... NaCl ⁄ chloroform (1 : 1
v ⁄ v). A new volume of chloroform was used for each of
the three extractions. Finally, the organic phase was
re-extracted with de-ionized water to remove traces of salts,
and ... Hofmann K & Stoffel W (1993) TMbase – a database
of membrane spanning protein segments. Biol Chem
Hoppe-Seyler 374, 166 .
44 Claros MG & von Heijne G (1994) TopPred II: an
improved software...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
Ngày tải lên :
18/02/2014, 16:20
... ¼½ðE
target
Þ
DC
ttargetðcontrolÀsampleÞ
=
½ðE
ref
Þ
DC
trefðcontrolÀsampleÞ
;
where E
target
is the ampli cation efficiency of the target or
gene of interest, E
ref
is the ampli cation efficiency of the
reference (ribosomal 18S) and C
t
is the crossing ... recovery of
cells after protein damage, by assisting the folding of
cytoskeletal proteins that are actively synthesized
and ⁄ or renatured under these conditions. The upregu-
lation of all of these ... protein L10, is a transcription cofactor that
inhibits activation of AP-1 transcription factors. QM
protein is implicated in the conversion of a broad vari-
ety of extracellular signals generated...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... of plasmin in the amidolytic
assay. However, if we use the k
p
⁄ K
m
ratio as a mea-
sure of the overall impact of stearate, it should be clas-
sified as an inhibitor of plasmin, the potency of ... effect of
random outliers. The probability distribution of the
parameters estimated using this robust evaluation
procedure was essential for the identi cation of the
statistical significance of the ... Light attenuation of oleic acid micelles in the presence of synthetic plasmin substrate. (A) Twenty microlitres of 1 mM Spectrozyme-PL
was added to 180 lL of various concentrations of oleic acid...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
Ngày tải lên :
18/02/2014, 17:20
... cut-off). Production of
rabbit antiserum raised against the recombinant protein
and affinity puri cation of the antibody were performed by
Hokkaido System Science (Hokkaido, Japan).
Collection of ... overnight. Complete digestion of genomic DNA con-
tamination in the total RNA was confirmed by lack of
ampli cation of a b-actin mRNA fragment by PCR using a
pair of primers (5¢ -ATCACCATCGGCAACGAGAG-3¢
and ... distri-
bution of mRNA expression and immunolocalization
suggests the potential function of OMM-64. In the
inner ear sacculus, the otolith is close to the proximal
side of the sacculus, and calci cation of...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
Ngày tải lên :
19/02/2014, 02:20
... for the rapid detection of hydrophobins
from a large number of small samples, we have
investigated the potential of intact-cell MALDI-TOF
MS (ICMS). Initial applications of ICMS on fila-
mentous ... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on malt
agar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... typical MALDI-
TOF mass spectra of four strains of H. koningii, T. long-
ibrachiatum, H. virens and T. atroviride. The spectra
Fig. 1. Alignment of the six hydrophobin protein sequences of H. jecorina....
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
Ngày tải lên :
19/02/2014, 07:20
... identi cation of
CYP93E1 as triterpene hydroxylase implies that the
function of other members of the CYP93E subfamily
are not necessarily related to flavonoid biosynthesis.
Fig. 7. GC-MS analysis of ... exhibit a wide range of structural diversity produced by a
sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-
tial origin of structural diversity of skeletons in their ... min.
After the addition of 0.25 mL of 40% potassium hydroxide
solution and 0.25 mL of methanol, the cell suspension was
boiled for 5 min. Products were extracted three times with
0.2 mL of hexane and evaporated....
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Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
Ngày tải lên :
19/02/2014, 07:20
... number of genes in U6A
and U6A-2 cells, IFN-treated U6A-VV-II cells showed
the down-regulation of a substantially larger subset of
genes.
Identi cation and characterization of a subset of
ISGF3-independent ... 1579
expression profile of cells expressing the VV-II mutant
form of STAT2 was more similar to that of U6A cells
than that of U6A-2 cells (Level 1). IFN treatment led
to the further segregation of the sample ... Missis-
sauga, ON, Canada) and relative quantification soft-
ware were used for all reactions. PCR reactions were
performed in a final volume of 20 l L containing 0.5 lm of
each primer and 5 lL template cDNA...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
Ngày tải lên :
19/02/2014, 07:20
... Rosenfeld RG (1997) Identi cation of a family of
low-affinity insulin-like growth factor binding proteins
(IGFBPs): characterization of connective tissue growth
factor as a member of the IGFBP superfamily. ... the
presence of 10 mm CaCl
2
, as described in Experimental
procedures. Of the nine cell lines tested, OVISE,
Fig. 1. Analysis of noncleaved and cleaved
forms of IGFBP-rP1 in conditioned media of
eight ... other hand, 10 ng of trypsin converted a major
part of the 33 kDa IGFBP-rP1 to the 25 kDa form,
but an increased amount (50 ng) of trypsin nonspecifi-
cally degraded both forms of IGFBP-rP1. These...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... in the absence of azide.
Ó FEBS 2004 Bioinformatics and mutagenesis of GH-57 (Eur. J. Biochem. 271) 2869
Bioinformatics of the glycoside hydrolase family 57 and
identi cation of catalytic residues ... study of GH-57, no detailed
alignment of the complete sequences of GH-57 members
has yet been accomplished. To date, only p artial or selected
sequences have been compa red [17–19]. An alignment of
GH-57 ... AL662. The analysis
of this sequence revealed that the encoded enzyme i s a
member of GH-57 [21,22]. The cloning and expression of apu
in Escherichia c oli has led to the production of a C-terminally
truncated...
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Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
Ngày tải lên :
19/02/2014, 16:20
... sign) of a cycle is
the product of the values (the signs) of its steps. The sign o f
the magnitude of a Ôminus combined autoinfluence path
of order pÕ equal those of the arithmetic product of its
Ó ... absolute magnitude of a
combined autoinfluence path is the product of all the rates
divided by the product of all the concentrations of its
species. Therewith the c oefficient of o rder p of the
characteristic ... the basis of the graphical analyses of the
characteristic equation and of t he method we develop here.
Inspection of Eqn 12 shows t hat in a ll coefficients a
p
the
term consisting of negative...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
Ngày tải lên :
20/02/2014, 01:20
... interaction of Yef1p with Utr1p is of
biological interest and may be related to the pro-
nounced requirement of both Yef1p and Utr1p in the
absence of Pos5p.
The elucidation of the localization of Utr1p ... The loss of YCp-UTR1 was con-
firmed by the Ura– phenotype of MK1219 (require-
Table 2. Effect of compounds on ATP-NAD kinase activity of
Yef1p. The effect of compounds on the activity of Yef1p ... abilities of LEU2, UTR1 and YEF1
for the growth defects of the pos5 mutant
To confirm the complementing ability of LEU2 for the
growth defects of the pos5 mutant, we examined the
growth of pos5...
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