assays for detection and screening purposes of a l arabinofuranosidases of families 51 and 54
Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... vaccine strain; lane 2: positive control of CDV wild-type strain; lane 3: uninfected cells control; lane M: DL2000 DNA Marker; lane 4: canine parvovirus; lane 5: canine adenovirus; lane 6: canine ... control of CDV In addition to traditional methods using virus isolation, several promising antigenELISA, AGP, and FA methods have been developed and evaluated However, these methods are laborious and ... cells, and uninfected cells by RT-nPCR (Fig 1) Sensitivity, applicability, and repeatability of the multiplex RT-nPCR Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification...
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Báo cáo khoa học: "INTEGRATING MULTIPLE KNOWLEDGE SOURCES FOR DETECTION AND CORRECTION OF REPAIRS IN HUMAN-COMPUTER DIALOG*" potx
... containing a repair and missed 97 sentences actually containing a repair Of the 500 that it proposed as containing a repair, 62% actually did and 38% did not Of the 62% that had repairs, it made ... to parse, as well as a different set of acceptable phrase types Patterns While acoustics alone cannot tackle the problem of locating repairs, since any prosodic patterns found in repairs are likely ... speaker's range, and most had considerable energy Thus when combined with the feature of a following silence of at least 60 msec, glottalization on syllables with sulficient energy and not at...
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Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
... assays BA did the mosquito inoculation experiments and IFA PJA did the multiplex PCR for clinical samples and participated in the sequence alignment SA did the MAC ELISA and HI assay for all clinical ... Sathish N, Manayani DJ, Shankar V, Abraham M, Nithyanandam G, Sridharan G: Comparison of IgM capture ELISA with a commercial rapid immunochromatographic card test and microwell ELISA for the detection ... it critically All authors have read and approved the final manuscript Acknowledgements Gurukumar KR acknowledges Council of Scientific and Industrial Research, Government of India, for providing...
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Guidelines for the early detection and screening of breast cancer ppt
... and survival analysis at Salmaniya Medical Complex, Bahrain Eastern Mediterranean Health Journal, 1999, 5(3):430–439 Abdul Hamid G, Tayeb MS, Bawazir AA Breast cancer in south-east Republic of ... developing and implementing the national health care agenda and programmes Early detection programmes allow for a more favourable prognosis for patients, offer increased and less toxic treatment ... between a cyst and a solid mass, to explore a palpable abnormality not clearly visible on a mammogram or to obtain a better view of a lesion that cannot be mammogrammed It is often the initial diagnostic...
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STUDY ON EPIDEMIOLOGICAL FEATURES, APPLICATION OF DIAGNOSTIC KIT FOR DETECTION OF TRYPANOSOMIASIS CAUSED BY TRYPANOSOMA EVANSI IN CATTLE AND BUFFALOES IN A FEW NORTHERN MOUNTAINOUS PROVINCES AND RECOMMENDATION FOR PREVENTIVE AND TREATMENT MEASURES
... gadfly The fly species all appeared in all places investigated and frequency of appearance was 100 % Table 3.6 Result of nomenclature, distribution and frequency of appearance of blood sucking flies ... habitat, clearing plants in each area, not letting water stagnate; composting manure in order to kill eggs and larva of flies and gad flies; housing animals with nets to prevent flies and gad ... that clinical signs in trypanosome infected buffaloes and cattle include falling and rising fever, emaciation, anemia, edema, corneal inflammation, swelling of the testes and testitis, swollen...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... validation of flow cytometry and T cell functional assays, which are generally more challenging We developed and validated HLA -A2 flow cytometry, IFNγ real time RT-PCR, and IFNγ ELISPOT assays ... linearity of dilution Due to the lack of a standard reference material, calibration standard curves were not evaluated for quantification of cellular IFNγ response Linearity of dilution was evaluated ... considered acceptable Data from 80 TIL cells spiked into two PBMC lots at 105 cells (low PBMC assay) were analyzed by two analysts each performed eight intra-day assays and 10 inter-day assays Cells...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... GCG ACA CCA GTT G TCA ACC ATG AGG TCA TGG CCA TA CCC CGG GTG AGT TCT TGC TYG A TTC CCC TGG AGA AGT ACT CCT CGA ACT AAA TCC ATA CCT AGC ACAC CAG TGG CGG GAC AAA CCA AC CTC TCC TGG AGA ACT CCT ACT ... was obtained from external laboratories (Table 2) This stool panel was applied to the Lightcycler assay and all the genotypes were detectable Detection and quantification of human NoV by Real-Time ... method allows a calculation of the minimum amount of NoV present based on cDNA values Ideally quantification of an RNA virus involves the use of an RNA standard However, RNA standards are not...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... GCG ACA CCA GTT G TCA ACC ATG AGG TCA TGG CCA TA CCC CGG GTG AGT TCT TGC TYG A TTC CCC TGG AGA AGT ACT CCT CGA ACT AAA TCC ATA CCT AGC ACAC CAG TGG CGG GAC AAA CCA AC CTC TCC TGG AGA ACT CCT ACT ... was obtained from external laboratories (Table 2) This stool panel was applied to the Lightcycler assay and all the genotypes were detectable Detection and quantification of human NoV by Real-Time ... method allows a calculation of the minimum amount of NoV present based on cDNA values Ideally quantification of an RNA virus involves the use of an RNA standard However, RNA standards are not...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... †National Collection of Type Culture, ‡Sal: Salmonella spp., §ST: Salmonella Typhimurium, Salmonella Enteritidis ∥ SE: Detection and quantification of Salmonella spp., Salmonella Typhimurium and ... Methods Bacterial strains A total of 128 bacterial strains (i.e., 110 Salmonella spp and 18 non-Salmonella) were used in this study (Table 1) Salmonella isolates consisted of 13 serotypes and isolated ... *Salmonella Typhimurium, †Salmonella Enteritidis, ‡not tested Detection and quantification of Salmonella spp., Salmonella Typhimurium and Enteritidis Table The evaluation of the multiplex real...
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báo cáo khoa học: " Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring Insulin-Like Growth Factor-1 in a Preclinical Model of Human Breast Cancer" doc
... light-controlled conditions in the University of Maryland, Baltimore animal facility and maintained in accordance with institutional guidelines approved by the University of Maryland, Baltimore Animal Care ... the mammary gland: lessons from transgenic and knockout models J Mammary Gland Biol Neoplasia 2000, 5:19-30 Rinaldi S, Toniolo P, Muti P, Lundin E, Zeleniuch-Jacquotte A, Arslan A, Micheli A, Lenner ... and change in impedance was measured for The impedance value for each IGF-1 measurement was normalized to the corresponding PBS baseline value Each sample was measured at least in quadruplicate...
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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
... no template; lane M: DL2000 DNA ladder Maker (TAKARA) ing temperature (Tm) The thermal profile for melting curve analysis consisted of a denaturation for at 95°C, lowered to 55°C for 30 s and then ... be a simple, sensitive, specific and rapid method for detection and quantitation of PRRSV in field samples Materials and methods Virus strain and field samples CH- 1a (GenBank access number: AY032626) ... specificity and sensitivity of one step SYBR green real-time RT-PCR Plot of the amplification of a 10-fold serial dilution of ch- 1a RNA to calculate the detection limit and sensitivity of real-time...
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Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps
... Sensitivity of the LAMP assay The detection limits of the assay were evaluated using tenfold serial dilutions of plasmid (pVP3) The plasmid DNA (2.8 × 1011copies/ l) was serially diluted tenfold, and l ... of GPV infection in both laboratory and field settings Materials and methods Goslings, tissues, virus, DNA, and standard plasmid DNA templates preparation Goslings, tissues, virus, and standard ... multocida (5 :A) , Salmonella enteritidis (No 50338), and Escherichia coli (O78) (Key Laboratory of Animal Diseases and Human Health of Sichuan Province) FQ-PCR was carried out as control assay Sensitivity...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... clade and directly in clinical specimens, and evaluated it with a large number clinical samples Using this assay, reliable diagnostic results can be obtained in a few hours, thus enabling timely ... Our assay failed to detect virus in a nasal swab and a throat swab (Table 1) This may be due to RNA degradation during long-term storage and multiple freezethaw cycles Tran Tan et al Virology ... results were obtained when analyzing 48 clinical samples from patients with either seasonal influenza or non-influenza respiratory illness Clinical evaluation was performed on 58 stored clinical...
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Báo cáo y học: "Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay" pdf
... device Lateral-flow immunochromatographic assay (LFIA) was manufactured as double antigen direct sandwich assay Devices (DCN, Carlsbad, CA, USA) for testing of up to 10 l of biological samples ... predictive values were calculated according to the appropriate formula Sensitivity was exclusively calculated within the RA group Specificity was calculated against rheumatic diseases and a healthy ... British BioCell International), were individually conjugated to goat anti-human IgG and IgM (Dianova, Hamburg, Ger- Page of many) and mixed Anti-human immunoglobulin colloidal gold conjugate was dispensed...
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AN INTEGRATED ATOM CHIP FOR THE DETECTION AND MANIPULATION OF COLD ATOMS USING a TWO PHOTON TRANSITION
... stable only if the magnetic moment of the atom adiabatically follows the direction of the magnetic field In both classical and quantum mechanics, the magnetic moment of atom adiabatically follows ... (2.21) Classically, θ is the angle between the magnetic moment and a magnetic field Quantum mechanically, for the Zeeman energy levels of a neutral atom the total angular momentum is F and the ... knowledge how to build laser and laser electronics, among many others I would also like to thank my all other colleagues in CQT, specially Evon, Teo, Dileej, Bob, Imran, Jacky, for your help and...
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Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids
... detection based on signal amplification approach Electrochemical and optical nucleic acids assays based on (I) a novel carbon nanotube (CNT)-based label, (II) a branched DNA (bDNA) amplifier have been ... follows: (I) CNT-based label A novel multiple enzymes conjugated CNT-based label has been developed for highly sensitive optical and electrochemical detection of human ALL related p185 BCR-ABL ... 3-APZ 3-aminophenoxazone ABL Abelson leukemia ABTS 2’azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid AFM atomic force microscope ALL acute lymphocytic leukemia ALP alkaline phosphatase AML acute...
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Exploration of a framework for behavior based malware detection and classification
... that malware behaviors are composed of basic behavior blocks that are shared mainly between malware variants of the same family, and among a small number of malwares in other families This means ... collected is semantically rich enough to allow the identification of known malwares and classification of malwares based the similarity of their behaviors, as will as flexible enough to allow ... malware actions This research also has the potential to allow malware family classification using another paradigm Finally, the information learned from future research in this area will help...
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A study of difficulties in learning english listening skill for beginners in the asemlink of intrernational languages center and some suggested solutions
... vital to have at least a native teacher of that language Many English centers offer foreigners such as American, Canadian, Australian or even Filipino, Singaporean people as teachers of English ... their foreign language acquisition Adults’ capacity for understanding and logical thought is greater, and they are likely to have developed a number of learning skills and strategies which children ... “situational” and “task” motivation Global motivation is the overall orientation of the learner towards the learning of the foreign language; Situational motivation has to with the context of learning...
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A reliability data base for performance and predictive assessment of electric power systems
... Committee on Outage Statistics, Canadian Electrical Asso~iation, Instruction Manual for Reporting Generation Equipment Outage Data, Canadian Electrical Association, Montreal Billinton R and Allan R N ... Probabilistic Approach", Transactions PWRS-1, No 4, pp 171-178 - 12 Billinton, R., Debnath, K., Oprisan, M and Clark, I M., April 1990, "The Canadian National Data Base for Reliability Evaluation and Assessment", ... reserve assessment and probabilistic evaluation of transient stability ( ) Billinton and Chu (11) The SRIS data base can provide almost all the basic data required in a probabilistic operating...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25