artery athymic nude mice in which angiogenesis is characteristically impaired received an intracardiac injection of 5×105 culture expanded human epcs hepcs two control groups were identically injected with either human microvascular ecs hmvecs or m
CELL SELECTION AND RESELECTION pot
... CORPORATION TTKTVT HCM CELL RESELECTION PROCEDURE MEASUMENTS FOR CELL SELECTION MS search tất kênh hệ thống (124 for P‑GSM, 174 for E‑GSM, 194 for R-GSM, and 374 for DCS 1 800) MS đọc sóng mang ... DIV 7) * BS_PA_MFRMS / 4} N: số sóng mang BCCH BA list Đọc từ tin “System Information Type 2” BS_PA_MFRMS: Số khung liên tiếp dành cho Paging đa khung 51 Đọc từ tin “System Information Type 3” ... 900MHz RXLEV(2) = -70dBm cell 900MHz RXLEV(3) = -75dBm cell 900MHz RXLEV(4) = -68dBm cell 1800MHz RXLEV(5) = -70dBm cell 1800MHz RXLEV_ACCESS_MIN = -98dB MS_TXPWR_MAX_CCH = 30dBm với MS 1800 MHz...
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Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
... this paper, we established cell suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods ... for 10-30 min, and then rinsed repeatedly with sterile distilled water The sterilized roots were cut into sections of 2-10 mm and then were inoculated into MS solid medium (Murashige and Skoog, ... times of subculture into the solid medium the callus were inoculated into liquid medium (same with above) Stock cell culture and culture condition Suspended cells of P vietnamensis were initiated...
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Báo cáo " Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production " docx
... plant cell cultures have been found [2,7] Optimization of the hormone concentration and combination are often effective For ginseng cell growth, 2,4 D is most commomly used in routine culture maintenance ... effective in increasing productivity In this paper, we established cell suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of ginseng cell culture ... on The maximum biomass yield of cell suspension culture of ginseng was obtained in medium containing 2,4-D as compared to IBA or NAA However, ginsenoside production was much higher in IBA or NAA...
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Báo cáo khoa học: Super life – how and why ‘cell selection’ leads to the fastest-growing eukaryote doc
... that may comprise at least four main metabolic processes, i.e catabolism, anabolism, maintenance and transport [911] Each of these metabolic groups consists of a network of interacting metabolic ... selective mineral media with substrate markers are used The drawback of using mineral medium is often a lower maximum growth rate, resulting in an increase in cost-intensive bioreactor times To optimize ... eukaryotic organism on dened glucose ammonium mineral medium, and is certainly Control of highest eukaryotic growth rate much shorter than that of the more minimalist Mycoplasma genitalium [60] Figure...
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Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
... After times of subculture into the solid medium the callus were inoculated into liquid medium (same with above) Stock cell culture and culture condition Suspended cells of P vietnamensis were initiated ... of ginseng cell was affected by concentration of macro elements in the MS medium Cultures were maintained in 300 ml conical flasks for weeks Concentration of macro element NH4NO3 KNO3 Determination ... through callus induction from the cultivated plant root [8] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose...
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Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
... Nur77 is part of a small family of transcription factors (which also includes Nurr1 and Nor1) that is thought to play an important role in mediating TCR-induced apoptosis in immature thymocytes ... Palmer for his support Work from the laboratory of MAD and ET are supported by the University of Missouri Mission Enhancement Fund References Anderson G, Lane PJ & Jenkinson EJ (2007) Generating intrathymic ... these and other roles of Akt and ERK2 [15], these data suggest an additional mechanism by which thymocytes could distinguish positive from negative selecting ligands The mechanism by which Nur77 mediates...
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Báo cáo y học: "VLA-4-dependent and -independent pathways in cell contactinduced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients" potx
... (CD11a-5E6, anti -human CD11a/LFA-1α; AZN-L27, antihuman CD18/integrin β2; Lia1/2, anti -human CD29/integrin β1; 5F12, anti -human CD44; ACT-1, anti -human integrin α4β7) were obtained through the VIth Human ... thank S Yamashita and M Komine for secretarial assistance This work was supported in part by a grant from the Japanese Ministry of Education and the Organization for Pharmaceutical Safety and ... enhance function of human B cells J Clin Invest 1998, 102:606-618 Mighell AJ, Thompson J, Hume WJ, Markham AF, Robinson PA: RT-PCR investigation of fibronectin mRNA isoforms in malignant, normal...
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Báo cáo y học: "B cells in Sjögren’s syndrome: indications for disturbed selection and differentiation in ectopic lymphoid tissue" doc
... populations of IgM (cµ)expressing memory B cells and memory-type B cells seem to be closely involved in both B-cell disturbances and malignant complications in pSS Finally, more than half of individual ... be abnormally stimulated by local BAFF excess and locally secreted (auto)antibodies Abnormal stimulation and impaired censoring mechanisms enhance the risk for malignant transformation of B cells ... structures within inflamed tissues remain unclear For example, in pSS, the following open questions remain about the role of ectopically formed lymphoid tissue in B-cell disturbances and autoimmune...
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Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx
... Cell culture supernatants were collected and tested for cytokines in enzyme-linked immunosorbent assay (ELISA) For determination of polyclonal IgM, B cells were cultured for days Animal studies MRL-MpJ-Faslpr/lpr ... determined in acridine orange flow cytometry Interleukin-6 (IL-6) and polyclonal IgM were measured in enzyme-linked immunosorbent assay (ELISA) (n = to 7) (e) Total mouse B cells were stimulated with ... mediumtreated samples) FITC, fluorescein isothiocyanate class B INH-ODNs as inhibitors of both anti-dsDNA antibody secretion (detected by immunofluorescence and by ELISA) and of anti-Sm/RNP antibody...
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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc
... Figure Serum immunoglobulin levels in pristane-treated mice Total immumice noglobulin levels were measured by enzyme-linked immunosorbent assay in serum obtained months after treatment with phosphate-buffered ... by, and performed in compliance with, the guidelines of the Institutional Animal Care and Use Committee Autoantigen microarrays Antigens were printed in ordered arrays on FAST slides (Whatman, ... pristane or PBS as a negative control WT BALB/c mice treated with pristane develop an immune complex-mediated glomerulonephritis [38] The development of proteinuria in the mice, a measure of...
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Báo cáo y học: " Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell" potx
... medium for >4 weeks before washing again and incubating in 50 μg ml-1 ampicillin, 50 μg ml-1 streptomycin dissolved in K/2 medium, incubating for days, and then transferring to antibiotic-free medium ... transport and metabolism Energy production and conversion Intracell traffic., secretion and vesicular transport Amino acid transport and metabolism Lipid transport and metabolism RNA processing ... 3.9 × 10-3 MORN3 _HUMAN MORN repeat-containing protein × 10-18 GS00985 7.8 × 10-3 PTHD2_MOUSE Patched domain-containing protein 2 × 10-08 7.8 × 10-3 AMT12_ARATH Ammonium transporter member 2 × 10-25...
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báo cáo khoa học: " Effect of clone selection, nitrogen supply, leaf damage and mycorrhizal fungi on stilbene and emodin production in knotweed" doc
... of the pots was inoculated with a mixed inoculum of isolates of arbuscular mycorrhizal (AM) fungi: Glomus mossae BEG95, G claroideum BEG96 and G intraradices BEG140 (all originated from man-made ... [22], antioxidant, antimutagenic, anti-inflammatory, chemopreventive [23,24] and anticancer effects [25-27] including the inhibition of breast cancer [28] It also inhibits aglucosidase which is ... 52 Majority of its particles fell in the category 10-50 m (50%), followed by 1002000 m (40%) months Each inoculated pot received 100 ml of inoculum of AM fungi, consisting of equal volumes of...
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Báo cáo y học: "Dendritic cell subsets dynamics and cytokine production in SIVmac239-infected Chinese rhesus macaques" pptx
... Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, ... remained at a normal level at the chronic phase of infection The trends of change were more likely with African green monkeys, but not with Ind Rhs This difference in change may be important in ... this study were from the Kunming Primate Research Center, Chinese Academy of Sciences (CAS), and housed at the ABSL-3 laboratory in accordance with the Guide for the Committee on Animals for...
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Báo cáo y học: "The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)" pdf
... liquid cultures of S coelicolor was shown to influence ribosome biogenesis, certain transport systems, and a number of genes from major carbon metabolic pathways, amino acid metabolism and purine/ ... Strand Synthesis Supermix (Invitrogen) according to the manufacturer's instructions PCR cycling was performed at 25°C for 10 minutes, 42°C for 120 minutes, 50°C for 30 minutes, 55°C for 30 minutes ... known involvement of ppGpp synthesis in the regulation of antibiotic and secondary metabolite production, and paint a picture of a global regulatory mechanism with inhibitory and stimulatory effects...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... treatment of NP Inflammatory mechanisms Inflammatory cells disorder is one of the most important mechanisms in NP pathogenesis Based on the genetic defection, the atopic immunity response to microorganisms ... transport protein A intraflagellar transport protein B immunohistochemical staining interleukins interleukins 13 interleukin xxii IL-9 IT KRT14 KRT5 LEKT1 LTC4S Math1 MCPH MFI MMP MTOC MUC1 MUC16 ... epithelial stem cells, it is still difficult to distinguish between the normal and abnormal mechanisms underlying epithelial repair and remodeling Therefore, culturing and comparing human nasal epithelial...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... passaging, by replacing NP medium with N2B27 medium made up of DMEM/F12 with mM L-glutamine and 1×N2 supplement and Neurobasal medium with 0.2 mM NEAA and 1× B27 supplement mixed in a 1:1 ratio (Ying ... F-12 medium (DMEM/F12) supplemented with 20% KnockOut Serum Replacement (KOSR), mM L-glutamine, 0.2 mM nonessential amino acids (NEAA), 0.1 mM 2-mercaptoethanol and ng/ml recombinant human basic ... inactivation of MEF cells MEF primary cells were plated on a gelatin-coated 15-cm tissue culture dish and expanded in MEF medium containing Dulbecco’s modified Eagle’s medium (DMEM) supplemented with...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... dynamically regulated, and are important during mammalian development In particular, the brain is one of the most developmentally evolved and complex biological systems It is comprised of distinct ... are decorated with H3K4me3 (trimethylation of histone lysine 4) and H3K27me3 (trimethylation of histone lysine 27) bivalent epigenetic marks, reminiscent of “poised” protein-coding genes in mESCs ... the chromatinmodifying complex to silence neuroectoderm lineage markers by H3K27 methylation Binding of lncRNA to SOX2 may prevent OCT4 or other core ES transcription factors from binding Since...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line (Figure 8.5) Since transcription factor binding events may not ... NPCs from which the neurons were derived from In Chapter VII, the indispensable role of RMST in human neurogenesis was confirmed in vitro, by means of an RNAi approach Neural progenitors with decreased ... other somatic tissues All three isoforms of RMST were detected by qPCR, and relative expression was normalized to RMST expression in the whole brain sample Microarray analysis indicated that RMST...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors Stem Cells 28, ... lncRNAs were established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance ... is also probable that hnRNPA2B1 may regulate the splicing of RMST into its three isoforms However, in this current study, the functions of individual isoforms are not established 8.3.2 RMST may...
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