... printer had insinuated itself within the AI Lab's network, however, something had changed. The machine worked fine, barring the occasional paper jam, but the ability to modify according ... factual input of various has grown. Since launching the GNU Project in 1984,5 Stallman has been at turns ignored, satirized, vilified, and attacked-both from within and without the free software ... cutting." For more information on the GNU General Public License, visit [http://www.gnu.org/copyleft/gpl.html.] In an information economy increasingly dependent on software and increasingly...
... Original (discontinuous) tree2. Binarization:Binarize discontinuous tree, op-tionally with Markovization3. Resolve discontinuity:Split discontinuousnodes into components, marked with indices4. ... the re-sulting PLCFRS, using the different binarizationschemes. First with a right-branching, right-to-leftbinarization, and second with the minimal bina-rization according to parsing complexity ... onbinarizing an LCFRS in a manner that min-imizes parsing complexity, but the presentwork shows that parsing long sentences with such an optimally binarized grammar re-mains infeasible. Instead,...
... a direct interaction of bacitracin with PDI, involvingdisulfide bond formation between an open thiol form of the bacitracinthiazoline ring and cysteines in the substrate-binding domain of PDI.AbbreviationsACN, ... 2011 FEBS 2035 Bacitracin inhibits the reductive activity of proteindisulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domainNina Dickerhof1, Torsten ... Darby NJ & Freedman RB(1998) The b domain provides the principal peptide-binding site of protein disulde isomerase but alldomains contribute to binding of misfolded proteins.EMBO J 17,...
... thedominant mode and in the case of the lupin enzyme itwas over 20 times slower. Such slower cleavage to give18 could arise either from weaker binding of 3 in theactive site of the hydrolase in ... with 0.1M KH2PO4, pH 6.0 (solvent A); solventA/methanol (9 : 1, v/v) (solvent B): 0–9 min, 0% B; 9–15 min, 25%B; 15–17.5 min, 90% B; 17.5–19 min, 100% B; 19–23 min, 100%B and 23–35 min, ... present the results of studies using novel diadenosinepolyphosphate analogs, with an oxymethylene group replacing one or twobridging oxygen(s) in the polyphosphate chain. These have been tested aspotential...
... presence of increasing concen-trations of methyl green, a majo r groove b inding drug [41]and distamycin A, a minor groove binding drug [42].Increasing concentrations of distamycin A resulted in adecrease ... hepatectomy interacts with complex C1,resulting in t he formation of c omplex C2. I f rRLjunRP i sinteracting with RLjunRP complexed with DNA, nosignificant difference in the footprinting pattern ... to incubate overnight. The strip was washed with three changes of 1 · binding buffer over a period of30 min and autoradiographed. In vitroDNase I footprinting analysisDNase I f ootprinting...
... program evaluation studies published in peerreviewed journals since 1990 demonstrating programeffectiveness in delaying first intercourse and/orincreasing the use of condoms or other contraceptivemethods ... The findings indicated that theprogram resulted in delayed first intercourse and/orincreased use of condoms or other contraceptivemethods.School-basedAarons, S.J., Jenkins, R.R., Raine, ... chlamydia infection (anincreasingly common STI among Canadian youth)can lead to severe medical consequences includingpelvic inflammatory disease (PID) and infertility,chronic pelvic pain, ectopic...
... proteins where zinc iscomplexed through four invariant cysteine and/or histidineresidues, forming a zinc finger domain that is mostlyinvolved in DNA binding but also in protein–proteininteractions ... selenomethionineand methylselenocysteine were inactive, as seen previously with XPAzf. As MT contains 20 zinc-binding cysteines ascompared to four cysteine s in XPAzf, for better compar-ison, zinc ... the coupling of zinc-binding structures (either in MT or in zinc finger proteins) by selenium compounds with the cellular r edox system GSH/GSSG, reducible seleniummay be regenerated within catalytic...
... mPER1 contains an additional NLS in its C-terminal domain. (A) Mapping of NLS function in murine Per proteins. Fragments corres-ponding to different portions of mPER1, mPER2 and mPER3 (as indicated) ... The immunostaining was performed with the myc-Cy3 or flag-FITC (Sigma). The cells were embed-ded with Vectashield containing 4’,6-diamidino-2-phenyl-indole (DAPI; Linaris, Bettingen, Germany).AcknowledgementsThis ... located in the C-ter-minal portion (fragment 4) of mPER1 within the 186C-terminal amino acids (Fig. 3, fragment 4b). Wealso noted faint nuclear signals for the correspondingC-terminal fragments...
... acknowledgements4Introduction 5Test 1Paper 1 Reading8Paper 2 Writing 17Paper 3 English in Use20Paper 4 Listening 29Paper 5 Speaking 34Test 2Paper 1 Reading36Paper 2 Writing 45Paper 3 English in ... 4 Listening57Paper 5 Speaking62Test 3 Paper 1 Reading64Paper 2 Writing73Paper 3English in Use76Paper 4 Listening85Paper 5 Speaking 90Test 4 Paper 1 Reading 92Paper 2 Writing 101Paper ... 2PAPER 1READING(1hour 15 minutes)Part 1Answer questions 1-16by referring to the magazine article on page 37, in which four naturalistsexplain their choice of most inspiring book about...
... 5Dystrophin IF after 15 days in culture. Antibody anti-dystrophin N-terminal in green fluorescence. a) DMD muscle cells, after 15 days in culture, with nucleus dyed with Bisbenzimide H33342 (negative ... cultured with differentiating human DMDmyoblasts and myotubes even when the co-culture wasmaintained in growth media [36]. The present results ofco-culture of CD34+ and DMD myoblasts without theinductive ... can interact andexpress dystrophin. This data together with our previousfindings [25] suggest that HUCB loose the capacity to fuse with muscle cells when they are previously committed. In other...
... Levin MF: Compensatory strategies for reaching in stroke. Brain 2000, 123:940-953.16. Levin MF: Interjoint coordination during pointing movementsis disrupted in spastic hemiparesis. Brain 1996, ... reality in clinicaltraining: developing the spatial skills of children with mobil-ity impairments. InIn Virtual Environments in Clinical Psychology andNeuroscience: Methods and Techniques in Advanced ... studying reaching and grasping in healthy sub-jects and in individuals with hemiparesis by comparingmovement kinematics of identical tasks made in a physi-cal and a virtual environment. Since...