approaches data analysis and inference
Population Genetics for Animal Conservation potx
... sections: Statistical Approaches, Data Analysis and Inference; Molecular Approaches and Applications; From Genetic Data to Practical Management: Issues and Case Studies and Future Directions ... Introduction [1] HEIDI C HAUFFE AND VALERIO SBORDONI Statistical approaches, data analysis and inference [23] Statistical methods for identifying hybrids and groups [25] ERIC C ANDERSON How to use MIGRATE ... 395–420 Young, A G and Clarke, G M (2000) Genetics, Demography and Viability of Fragmented Populations Cambridge: Cambridge University Press Statistical approaches, data analysis and inference Statistical...
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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
... specificity Materials and methods Animals Sprague–Dawley rats (two male and two females, 250– 270 g) and C57B1/6 mice (two adult males and two adult females, one wild-type and one Adh4 knock-out ... (Belmont, MA, USA) and the Netherlands Brain Bank (Amsterdam, The Netherlands) Blocks of cortex, anterior amygdala, striatum and midbrain from four nondemented control subjects (three male and one female, ... metabolism of ethanol and retinoic acid In vitro and in vivo data indicate that ADH1, ADH3 and ADH4 can oxidize retinol to retinal, with ADH4 having very high efficiency and ADH3 low efficiency...
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Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx
... at exons and 2, and and of the AANAT gene we subjected RNA from mouse tissue and normal and malignant melanocytes to RT-PCR amplification (Fig 3A) RT-PCR with primers located at exons and demonstrated ... cycle and skin cell type In this study, we show the specific expression of the tryptophan hydroxylase gene in normal and malignant mouse melanocytes, in anagen and catagen skin, and in pituitary and ... authentic standards identified elution peaks for N-acetylserotonin and melatonin Protein concentration was determined with a dyebinding method using BSA as the standard [24] Western blot analysis...
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báo cáo hóa học: " Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord" potx
... Foundation (KK) and by NIH grants PO1 HD25938 and RO1 CA95287 (WBS) We thank Dr Michael Hefferan and Dr Viktor Skihar for help with spinal cord surgery, and Francisco Beltran and Adriana Charbono ... with Adobe Photoshop CS3 Ver 10.0 (Adobe Systems) to standardize brightness and contrast All data were analyzed statistically using ANOVA and un-paired t-tests P-values less than 0.05 were considered ... types during demyelination and remyelination Along with comparisons of demyelination and remyelination in wild type and NG2 null mice, we evaluated the recruitment and abundance of specific cell...
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báo cáo hóa học:" Progressive obesity leads to altered ovarian gene expression in the Lethal Yellow mouse: a microarray study" pptx
... CH, and KT carried out the treatments and tissue collection, prepared preliminary data summaries, and participated in microarray analyses KE performed RNA extractions and microarray analyses, and ... data JB performed final data analysis and drafted the manuscript All authors read and approved the final manuscript Acknowledgements Grant Support: NIH INBRE 2P20RR016479, NIH R15 HD044438, and ... These data will be presented in a separate communication Figure ± SEM; n = for each group) Body weight (g) of 90 and 180-day black and LY mice (mean Body weight (g) of 90 and 180-day black and...
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Báo cáo y học: "Hepatocyte growth factor ameliorates dermal sclerosis in the tight-skin mouse model of scleroderma" doc
... of HGF on IL-4 and TGF-β1 mRNA expression in tight-skin mice IL-4 and TGF-β1 mRNA expression in tight-skin mice Total RNA was isolated from the skin, spleen, and lung, and IL-4 and TGF-β1 mRNA ... participated in the design and co-ordination of the study, and participated in the interpretation of the results T Imado and SK performed the animal study and histologic analysis HS participated ... of IL-4 and TGF-β mRNA expression and production IL-4 and TGF-β have been postulated to have major roles in fibrinogenesis in animal models [19-23] To clarify whether modulation of IL-4 and TGF-β...
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Báo cáo y học: "Analysis of C4 and the C4 binding protein in the MRL/lpr mouse" pptx
... interpreting the data, and critically reviewed the manuscript MCB acquired funding, planned and supervised the experiments, and revised and edited the manuscript All authors read and approved the ... infiltrates, and increased C3 and IgM staining The patient was diagnosed with atypical Behcet's disease and was treated with solumedrol and cyclophosphamide Genotyping was not reported, but her father and ... changes in histologic score and immunofluorescence data Comparisons of serum C4 levels were analyzed by analysis of variance with a Bonferonni P value correction Kaplan–Meier analysis was performed...
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báo cáo khoa học: " Increased hepatic oxidative metabolism distinguishes the action of Peroxisome proliferator-activated receptor d from Peroxisome proliferatoractivated receptor g in the ob/ob mouse" ppt
... ion (184 m/z) Multivariate data analysis was performed using SIMCA-P+ 11.0 (Umetrics AB, Umeå, Sweden) NMR data sets were mean-centered and Pareto-scaled prior to analysis Pareto scaling involves ... variable’s group standard deviation and therefore does not bias models towards large concentration metabolites Data sets were analyzed using principal components analysis (PCA) and partial least ... metabolomic and chemometric studies, and drafted the manuscript DGH and DWA coordinated the animal study and sample collection JNH participated in intellectual discussion of the data AWN participated...
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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
... similar to that used by Gao and Innan [10] (the collection of these genes is denoted as the NEW gene set; see Materials and methods, below, for detail and Additional data files and for the gene list) ... guage [48] Data were loaded into a MySQL database for subsequent querying Additional data files The following additional data are available with the online version of this paper Additional data file ... duplications in the human and mouse genomes, and estimated the respective duplication rates of the two mechanisms We conducted our analysis in both two-copy gene families and in the entire genome...
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Báo cáo y học: " Gene expression regulation in the context of mouse interspecific mosaic genomes" pdf
... between B6 and the IRCSs, B6 and SEG, and SEG and the IRCSs (Tables and 3) When partial correlations were considered, we conserved a significant correlation between B6 and the IRCSs and, interestingly, ... Materials and methods) The horizontal lines represent a 1% probability of random occurrence estimated by one thousand random permutations of gene order, for each strain and each chromosome and SEG ... the dysregulation between B6 and SEG on the one hand and B6 and the IRCSs on the other hand is associated with a strong correlation between gene expression in SEG and the IRCSs, meaning that the...
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Role of SPHK2S1P signalling in regulating mitochondrial function in the MPTP induced mouse model of parkinsons disease and in the MPP treated MN9D cells
... thesis I am extremely thankful and grateful to Professor Charanjit Kaur for her encouragement and moral support throughout my candidature She has been a pillar of strength and a source of inspiration ... Mrs Nandhini kanagaraj and Ms Ooi Yin Yin for their friendly support throughput the course of my study I owe my deepest gratitude to my parents for their eternal love, support and understanding ... examined Tyrosine hydroxylase (TH )and dopamine transporter (DAT) are two major rate limiting enzymes in the dopamine synthesis Gene expression analysis and Western blot analysis from the SNc of the...
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Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc
... on tissue Gb3 GSLs were extracted from kidney and liver of control and CsA-treated Fabry mice after weeks recovery post ERT, and from CsA-treated and untreated wildtype mice The Gb3 content was ... lanes and 3, GSL standards: from the top GlcCer, LacCer, Gb3,Gb4,Gb5 (Forssman), GM3, GM2,GM1; lanes and 4, lipid extract of wild-type liver; (F, G) lane 1, GSL standards; lanes and 4, lipid extracts ... wild-type and Fabry mouse tissues GSLs were separated by TLC (C ⁄ M ⁄ W 65 : 25 : v ⁄ v ⁄ v) and visualized using orcinol spray for carbohydrate (A) or VT1 overlay (B) to detect Gb3 Lane 1, GSL standards,...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... (wild-type) and 5¢-TTTTCTC TTTCCCCGTAAGGAAAGCAGAG-3¢ (mutant) The double-stranded oligonucleotides were labeled with [c-32P]ATP (Amersham Biosciences) and T4 polynucleotide kinase (TaKaRa), and used ... excised and DNA fragments were extracted Subcloning the fragments into pGEM-T easy vector followed by sequence analysis revealed that major and minor transcription start sites are 332 bp and 276 ... (Promega), which lacks eukaryotic promoter and enhancer sequences A series of deletions was prepared by use of restriction sites (PstI, XbaI, and SalI) (Fig 1) and by the method using exonuclease III...
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Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... motifs: the Walker A and B motifs, the signature or LSGGQ motif and the A-, D-, H- and Q-loops These motifs are positioned around the bound nucleotide and help to position and maintain it in the ... single-site mutants (E552D, N and A, and E1197D, N and A,) and three double-mutants (E552Q ⁄ E1197Q, E552Q ⁄ K1072R, K429R ⁄ E1197Q) The ATPase activity, binding affinity and trapping properties were ... analyzed E552Q (E552N and E552A) and E1197Q (E1197N and E1197A) mutants In the double-mutants Q ⁄ Q, R ⁄ Q and Q ⁄ R, trapping appears to be drug stimulated but Vi independent and occurs most readily...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
... structures of CMP and UMP, and interactions between the cytosine moiety of nucleotide and various side chains of wild-type CMP kinase (Upper) Chemical differences between CMP and UMP are indicated ... or dCMP showed that discrimination between CMP and UMP is achieved by Ser36, Arg110 and Asp132, which form hydrogen bonds with the amino group and the N3 atom of the cytosine (Fig 1) This study ... or various NMPs (CMP, dCMP, AraCMP and ddCMP), underlined the residues involved in recognition of the nucleobase, pentose moiety and phosphate group(s) [15], and site-directed mutagenesis experiments...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... Glu, Ser and Thr residues, the absence of Lys, His and Arg except at the termini, and the fact that it is completely unstructured based on the absence of medium- and long-range NOEs and the observation ... including the ‘BG loop’ and the ‘G’-strand (Fig 2) [24] Hortner et al [25] have modelled the structure of the SOCS3 SH2 domain and suggest that the BG loop and bG strand are formed from residues ... information available for the ESS, but sequence analysis, modelling [7] and the slight homology shared between the ESS and similar regions on Stat1 [8] and Stat3b [9] suggest that it may consist of...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
... interactions with substrates and ⁄ or inhibitors Results and Discussion Characterization of cDNAs and trypsin-like deduced protein sequences The three partial cDNA fragments and the 5¢ and 3¢ ends obtained ... expected size were identified by PCR analysis (T3 and T7 primers of TOPO TA CloningÒ Kit) and restriction enzyme analysis (EcoRI), followed by agarose gel electrophoresis, and sequenced from both directions ... crustacean trypsins [2,11] In addition, we hypothesize that Cys71 and Cys252 in Patry1a, PaTry1b, PaTry2 and PaTry3 sequences, and Cys71 and Cys267 in PaTry4 are free Cys As in crayfish [8], there is...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... mutant DNase I and then incubated with [35S]Met and [35S]Cys (A) The protein was immunoprecipitated from conditioned medium with rabbit anti-DNase I serum and protein A–agarose beads, and the eluant ... protein, and lanes and are endo-b-N-acetylglucosaminidase-treated and PNGase F-treated samples, respectively DNase I having different types of N-glycan is usually detected as a broad band [19] ... and transported through the endoplasmic reticulum (ER) and Golgi apparatus via a degradation and processing pathway (D) It may be salvaged from lysosomal degradation of oligosaccharide (D1) and...
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Báo cáo khoa học: Identification of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockfish Sebastes schlegeli pot
... 213 and 279 nm and the minor peak at 223, 267, 371 and 447 nm (data not shown), suggested the major peak to be a proteinaceous component, and the minor peak a flavin-like chromophore In native and ... spectra (A) and mass spectra (B) of FAD standard and the chromophore derived from SSAP The chromophore was obtained by boiling SSAP in 1% SDS for 10 min, ultrafiltration using an Ultrafree-MC, and RP-HPLC ... M A FAD standard Chromophore from SSAP 150 Absorbance 100 75 50 37 250 128 100 350 400 Wave length (nm) FAD standard 450 500 786.30 B 348.62 Intensity (%) mass of 120 kDa SDS ⁄ PAGE analysis revealed...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... nondenaturing polyacrylamide gel and found that fragment A, B and D clearly separated into two bands from which the upper band always corresponded to a rat brain control (Fig 2) Both bands from F-11 fragment ... separated into two bands The slower migrating band always corresponded to the rat brain control, and the faster migrating band to the N18TG2 control (Fig 5) The existence of both rat and mouse VRL-1 ... rVRL-1-GFP band appeared at 110 kDa, the upper band at 114 kDa and the diffuse band at 128 kDa The endogenous VRL-1 can also be observed at 80 kDa in the anti-VRL-1 Western blot and does not...
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