application of full length cdna resources to gain of function technology for characterization of plant gene function
báo cáo khoa học: " Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response" docx
... incomplete, the functional characterization of partial cDNAs in the library still allows the retrieval of sequence data for further experiment design and for the isolation of the full- length cDNA of specific ... expected number of genes for a higher plant [63,64]; The calculated number of 50698 transcripts in cassava is a guide for the future efforts required for the completion of the gene catalog for this ... We determined that the full- length library includes transcripts of a total of 7796 distinct genes, with alternative transcripts of about 26% of them To find the number of new transcripts captured...
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báo cáo khoa học: " An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library" ppsx
... sequence of castor FAD2 shares a high level (74%) of identity to that of the FAH12 (Fig 3) To confirm the functional identity of the castor FAD2 cDNA, we have cloned the corresponding ORF into the ... manufacturer's protocol Consequently, a full- length cDNA library containing ~5 × 105 clones was obtained Sequencing of a full- length cDNA library For sequencing, the cDNA library was transferred into the ... Reverse-genetic analysis by knockout or knock-down of these genes in castor plant may provide an answer to the function( s) of the acidic lipases in developing seeds, as transformation technology...
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Báo cáo y học: "ilot Anopheles gambiae full-length cDNA study: sequencing and initial characterization of 35,575 clones" pps
... program to increase the total amount of experimental sequence data and improve current Anopheles gene models Unlike EST data, full- length cDNA libraries are biased toward complete copies of mRNA ... some of the genes described here had previous EST evidence, the availability of full- length enriched cDNAs for these transcripts is particularly valuable for future annotation One transcript of ... availability of a genome from a mosquito relative, species-specific genes, along with the variability inherent in 5' and 3' exons, will probably require the use of full- length data for accurate gene characterization...
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Báo cáo khoa hoc:" Application of a biotin functionalized QD assay for determining available binding sites on electrospun nanofiber membrane" pdf
... method used for attaching antibodies to PVC-COOH nanofibers Page of The purpose of this study was to develop a new method for determining the amount of avidin protein covalently attached to complex ... spectrophotometer femtomoles of QD bound to avidin on the surface for the different weights of nanofiber mats as determined by the equation (fluorescence intensity- 5.3062) ÷ 0.6907 = femtomoles of ... of novel high performance platforms for protein blotting applications J Biol Eng 2007, 12 Senecal A, Magnone J, Marek P, Senecal K: Development of functional nanofibrous membrane assemblies towards...
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Tài liệu Status Report on The Application of Integrated Approaches to Water Resources Management ppt
... the results ofof the global monitoring of progress towards MDG target C: C: to halve, by 2015, results the global monitoring of progress towards MDG 7 target to halve, by 2015, results of the global ... areas for improvement that will take place over time (Armenia, Ghana) or lack indicators to assess performance of the instruments (China) This lack of monitoring tools to assess the performance of ... of the global monitoring of progress towards MDG 77target C: to halve, by 2015, the global monitoring of progress towards MDG target C: to halve, by 2015, the proportion ofof the population without...
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Báo cáo sinh học: " Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene" potx
... aMPV N gene (Fig 2) were utilized to generate affinity-purified rabbit peptide antibodies This approach was exploited to determine if any of the alternative start sites of the aMPV N gene were ... transcripts of influenza virus gene segments and are spliced within the nucleus for production of two different sizes of mRNAs sharing the same 5'-proximal AUG initial codon [16] The P gene of Sendai ... phosphoprotein (P) gene Transcriptional modification of the NDV P gene mRNA allows for potential expression of two smaller putative proteins, designated V and W [12], that appears to be a result of polymerase...
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báo cáo hóa học:" Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene" potx
... aMPV N gene (Fig 2) were utilized to generate affinity-purified rabbit peptide antibodies This approach was exploited to determine if any of the alternative start sites of the aMPV N gene were ... transcripts of influenza virus gene segments and are spliced within the nucleus for production of two different sizes of mRNAs sharing the same 5'-proximal AUG initial codon [16] The P gene of Sendai ... phosphoprotein (P) gene Transcriptional modification of the NDV P gene mRNA allows for potential expression of two smaller putative proteins, designated V and W [12], that appears to be a result of polymerase...
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báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)" doc
... unigenes compared to what they report of 10581 unigenes Meanwhile, almost 78% of the individual full- length EST unigenes had homology to a soybean gene of known or unknown function (3280 out of ... was found to increase the proportion of unigenes in certain gene ontology categories compared to other libraries For example, it was notable that the number of total unigenes of the full- length ... synthesis of even longer full- length cDNAs, and higher representation of long full- length cDNAs in the library In summary, this method of selecting full- length cDNAs by biotinylation of the mRNA...
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báo cáo khoa học: " A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica" pot
... alternative for gaining information about the expressed genes of an organism [6] In particular, the ESTs from a full- length enriched cDNA library provide the complete sequences of functional proteins ... ( & Number of superunigenes $ Maximum number of ESTs per contig Average number of ESTs per unigene Maximum unigene length Minimum unigene length ( Average unigene length ' & $ ( ... poplar, and times that of sorghum Full- length enriched cDNA library construction To construct a full- length enriched cDNA library containing highly variable sequences, total RNA was extracted...
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An analysis of key changes under ucp 600 compared to ucp 500 and recommendations for better ucp 600 application.doc
... receipt of the letter First of all, exporters should make sure that the letter of credit states it is subject to the 2007 revision of the Uniform Customs and Practice for Documentary Credits (UCP) of ... seller performs his other obligation, there will be no need for the buyer to draw against the standby letter of credit, which supports the obligation Back -to- Back letter of Credit Back -to- back ... buyer as collateral Figure 1: Back -to- back letter of credit transaction Many banks are reluctant to issue back -to- back letters of credit due to the level of risk to which they are exposed, whereas...
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The application of games in teaching grammar with reference to tieng anh 10 textbook at ha trung high school, thanh hoa province
... Tips for Using Grammar Games in Class Successfully - Organization: The best way to gauge this is to pay attention to students for the first few days to see what length of time works the best for ... understanding of Grammar, students would not be able to anything more than utter separate items of language for separate functions The expression of functional language is only possible through the use of ... and the aims of the study, it is necessary to limit the study to the areas which are manageable Benefits of the application of games in teaching grammar create the effectiveness of students’...
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An application of alternative risk measures to trading porfolios
... extended to two risk factors, while the portfolio consists of vanilla options on stocks 3.1 One Risk Factor To facilitate a better understanding of the behaviour of generalized Pareto distribution ... Equity stocks The risk factor under consideration is the one-day log-returns of the stock prices 3.1.1 UBS stocks We start with the analysis of UBS stock returns, a case of a highly liquid stock ... the analysis of the DAX stocks Given the lack of extreme events in the time period considered (October 4, 1988 to October 1, 2003), when fitting the tail to the generalized Pareto distribution...
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Application of Hydrothermal Reaction to Biodegradability Improvement of Refractory Pollutants: Structural Conversion of Di- and Trichloroacetic Acid to Biodegradable Products
... treatment methods For future, further inspection of hydrothermal reaction is also required to clarify reaction mechanism of other kinds of refractory pollutants and to apply this technology to real wastewater ... the number of attached chlorine atoms, regardless of the concentration of refractory parts in CAAs The results could be applied as basic data to the treatment of Chlorinated refractory pollutants ... conditions of 250 oC and MPa, biodegradability of DCAA increased to 0.54 within min, and did not vary much up to reaction time of 57 In case of TCAA, the biodegradability change was similar to that of...
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An application of CLT principles in teaching speaking to non english majors at nghe an economics and technology college
... teacher is like the skillful conductor of an orchestra, giving each of the performers a chance to participate and monitoring their performance to see that it is satisfactory At the final stage, the ... students to have capability of oral communication It also purposes to make students accustomed to listening and speaking Here, the students are provided and directed to conform the outline of the story ... boring topics In fact, some of the topics in the textbook were thought to be too difficult and less interesting to the students In this case, it is the role of the teachers to modify and bring to...
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Tài liệu KRONE - The effect of errors on TCP Application performance (Full version) docx
... performance of the application which is dependent upon TCP to provide the transport platform for delivery of its data Physical level errors will cause the application to wait for TCP to retransmit ... referred to by the acronym RTO for retransmission timeout It is calculated for each outgoing segment by a rather complex algorithm For our purposes, it is sufficient to know that the RTO is generally ... on the two types of protocols Connectionoriented protocols are sometimes called reliable protocols This is because they make provisions for important functions to be conducted; functions that...
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Tài liệu Mapping TOEFL® iBT Scores to the CEFR: An Application of Standard ... pdf
... scores to B1 through C1 levels for all four language skills • Listening and Reading judged to be too challenging for threshold A-level candidates • Writing judged to be too challenging for A1 ... wide range of topics in field of interest Sustains conversation with degree of fluency and spontaneity Takes listener and cultural context into account Speaks without causing undue stress to the ... just performing at each CEFR level – Done for Writing, Speaking, Listening, Reading • During Meeting – Calibration to CEFR Levels – Consensus on skills expected of candidates just performing...
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AN ACTION RESEARCH ON THE APPLICATION OF COOPERATIVE LEARNING TO TEACHING SPEAKING TO THE SECOND YEAR SUDENTS AT THE DEPARTMENT OF ENGLISH, GIA LAI TEACHERS' TRAINING COLLEGE
... the first and foremost objective of the study is for the sake of the students Besides, the study is expected to serve as a source of reference for teachers of English on the teaching of speaking ... in to CL will hopefully serves as an advisable supplementation to my knowledge on CL theory and CL application to English teaching For all the above reasons, it is strongly desirable for me to ... appreciation of the value of diversity In CL, groups often stay together for five weeks or more To achieve heterogeneous groups for speaking activities, teachers might want to look at their class...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose