antigen specific induction of regulatory t cells in vivo and in vitro
Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... death, TR cells lead to the induction of T regulator (Tr1) cells and Th3 cells, which feed back to inhibit inflammation, and the TR cells inhibit proliferation of antigen- specific and bystander ... differentiating into TR cells following activation are derived from a separate lineage of CD4+ T cells, and whether their induction is a result of the initial activation early in the inflammatory ... question: Do TR cells represent a lineage of T cells or a state of activation that may be achieved by any T cell under the appropriate conditions of activation? The induction of TR cells in the...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... and mature DCs, resulting in tolerogenic interaction with T cells in SIT We found that the combination of DNA vaccination with Fc and OVA had better effects onOVAinduced alterations in the spleen ... pathway of regulation of type immunity in asthma Competing interests The authors declare that they have no competing interests Authors' contributions YL performed all analyses and wrote the initial ... priming of antigen- specific CD8 +T cells in vivo[ 29,30] However, DCs express both activating and inhibitory FcRs, and the balance between activating and inhibitory signaling will determine whether...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... GITR-ligand interactions, abrogating the suppressive effect of Treg cells [37] With this in mind, it could be interesting to investigate whether the accumulated Treg cells in patients with arthritis ... one hand, transfer of Treg cells 24 hours after intra-articular antigen challenge might be too late to inhibit activation of effector T cells and their migration to the joint Indeed, T- cell activation ... of the T- cell compartment is still activated 14 days after intra-articular antigen challenge in the absence of Treg cells Compatible with these findings is that the production of cytokines in...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... amounts of IL-4 and IL-10.35 Further studies are required to elucidate the in vivo role of these cells and their subsets Effects of SIT on Dendritic Cells To understand the mechanisms of action of ... applicability that will have persistent clinical effectiveness that can be built within a short duration of therapy time.1 107 Early and Late Effects of SIT on Mast Cells, Basophils, and Eosinophils SIT ... from the oral mucosa were not able to transfer tolerance, but they acted as antigen- presenting cells in senso stricto irrespective of the source and route of SIT Induces Specific T- Cell Tolerance...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
... might not be a feasible option treatment decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation ... that Treg isolated from patients with active RA before treatment with anti-TNF-α were unable to suppress proinflammatory cytokine secretion from activated T cells and monocytes After anti-TNF-α treatment ... indicated that antigen- specific (HC-gp39) CD4+CD25+ Treg might be deficient in patients in comparison with controls [23] A more recent study indicated that in a restricted group of RA patients orally...
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Báo cáo y học: "haracterization of regulatory T cells in urban newborns" pptx
... supervised patient recruitment for the study and obtained funding JEG participated in study design, data analysis, and obtained funding All of the authors participated in drafting the manuscript and approved ... using SAS, version (SAS Institute, Cary, NC) and the R system for statistical computing [27] Results Subject characteristics The subjects in this study consisted of a subset of newborns and mothers ... differences in the numbers of CD25+bright cells present in CB and PB, we next sought to establish whether or not these CD25+bright cells expressed distinct patterns of differentiation/activation markers...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... stimulating T cell responses, stability of the phenotype following in vivo administration, the ability to migrate to sites of T cell activation and generation of CTLs Activated and mature DCs results ... cycles of vaccination (the time point associated with the maximal tetramer + CD8+ response, in patients undergoing vaccination with class I peptides only),, in vitro cytotoxicity against both peptides ... Introduction Induction of an effective anti-tumour response requires the active and integrated participation of host dendritic cells (DCs), taking up tumour-associated antigens (TAAgs) and generating...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... determining immunity or immune tolerance; this determination is based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen ... expression and inhibit T- cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction ... Arthritis Research & Therapy Vol 10 No Park et al deletion of antigen- specific T cells In contrast, repeated feeding of a low dose of antigen favors the induction of active immune regulation involving...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
... T cells after culturing in presence both IL-1b and IL-6 (C) The representative dot plots of CD39+Tregs and Th17 cells detected in naïve CD4+ T cells after culturing in presence of TGF-b1 (D) The ... human Tregs inhibit the generation and differentiation of Th17 cells is unknown It was reported that murine Tregs inhibit Th17 cell responses in vivo in a signal transducer and activator of transcription-3-dependent ... cells were Tregs The most important finding in the present study was that CD39+Tregs could be able to inhibit the generation and differentiation of Th17 cells in a dose-dependent manner The mechanism...
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Báo cáo y học: "CD134 as target for specific drug delivery to T cells in adjuvant arthritis" pps
... experiments and participated in the interpretation of the results WVE participated in the design of the study and in its coordination LE participated in the experiments, statistical analysis and interpretation ... about this time T cells start to infiltrate the joints [17] The present data therefore suggest that early in AA, CD134+ T cells, after activation in the PLN, can migrate to Available online http://arthritis-research.com/content/7/3/R604 ... by treatment with drug-containing anti-CD134 liposomes Next, we investigated whether local targeting to CD134+ T cells in the joint-draining PLN would affect the course of actively induced arthritis...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
... intact in cultures without antigen- presenting cells The functional activity of both patient and healthy control Tregs, however, was decreased in the presence of the patient’s antigen- presenting ... Overproduction of type I interferons in SLE matures dendritic cells (DCs) into immunogenic antigen- presenting cells that strongly contribute to the T- cell and B-cell hyperactivity characteristic of this ... not be able to overcome the strength of T- cell stimulation or the inhibitory effects of proinflammatory cytokines produced by other cells It is known that strong Toll-like receptor stimulation...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
... surveillance and JE pathogenesis Since there is no evidence for JEV infection of DCs in vivo, we investigated the alteration of phenotype and function of the JEV P3-infected DCs both in vitro and in vivo ... IFN-a and TNF-a was independent on viral replication DCs infected with P3 attenuated allostimulatory activities to T cells To test whether P3 infection will impair the ability of DCs to activate ... attempted to address the possible pathogenesis of JEV wild strain infection by testing the interaction of JEV and DCs in vivo and in vitro Carrasco et al., [2004] discovered that CSFV could infect...
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The role of CD8 t cells in the differentiation of TNF iNOS producing dendritic cells and TH1 responses
... Abstract In this study, we showed that activated human CD8 T- cells could induce DCs to produce IL-12p70 in vitro and this interaction also resulted in the production of a cytokine milieu that ... each individual lymphocyte B cells and T cells are the major types of lymphocytes T cells provide important cell mediated immunity by participating in the cytolysis of infected cells through the ... unprecedented ability to activate naive T cells (Steinman and Cohn, 1974) These cells are now known as the primary instigators of the adaptive immunity as they are vital for detecting, alerting and...
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Báo cáo y học: "β Transforming growth factor-β-induced regulatory T cells referee inflammatory and autoimmune diseases" potx
... CD4+CD25+ Treg are potent inhibitors of innate/adaptive immunity [61–63], induction of a population of Treg and documentation of their in vivo potential was an important next step In pursuit of this ... aberrant immunity In mediating its suppressive effects, TGF-β signals through the type I and type II TGF-β serine–threonine kinase receptors, T RI and T RII Interaction of the TGF-β ligand with these ... transfer of in vitro TGF-β-converted Treg together with ovalbumin -specific TCR transgenic T cells resulted in a profound inhibition of antigen- specific expansion of naive CD4+ transgenic T cells in vivo...
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Báo cáo khoa học: "Effects of propranolol in combination with radiation on apoptosis and survival of gastric cancer cells in vitro" ppsx
... used to quantify the cytotoxicity of gastric cancer cells induced by treatments The cells were plated in six-well plates (Costar, USA) at low densities After overnight culture, the cells were treated ... CTGGGCTGTTCTCGCTTCG CTCTCCTCTTCCTTCTCTTCTTCC 140 NM_001025370.1 EGFR 53 Forward Reverse AGG ACA GCA TAG ACG ACA C AGG ATT CTG CAC AGA GCC A 90 NM_005228.3 secondary antibody corresponding to the ... Dunnett’s test Those p values that were less than 0.05 were considered statistically significant Analysis of the data and plotting of the figures were performed with the aid of software (Origin...
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Báo cáo khoa học: " No supra-additive effects of goserelin and radiotherapy on clonogenic survival of prostate carcinoma cells in vitro" ppsx
... interpreted the data and helped drafting the manuscript MN and AH did the irradiations and performed statistical analysis PT and HS participated in the study design and interpretation of the data HC ... cannot be attributed to an increase in radiosensitivity of PC cells by goserelin in vitro It is to be explained by the synergistic effects of AD and irradiation Although studies on the combinatory ... withdrawal did We investigated the influence of incubation with goserelin on the radiosensitivity of PC cells in vitro This is of particular interest, as about 85% of PC express LHRH-receptors...
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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
... between the in vivo and in vitro situation are difficult to make However, the CSC concentrations used in this study are comparable to the concentrations used in other in vitro studies In our study ... on the cellular effects of smoke is based on studies using such smoke extracts instead of smoke Notwithstanding the inherent limitations of this model, it can be argued that epithelial cells in ... content To investigate the effect of CSC on intracellular GSH in the epithelial cells used, the intracellular GSH content of NCIH292 cells was assessed at different times after exposure to CSC The...
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Báo cáo y học: "Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis" docx
... receptors In comparison with intracellular cytokine staining, the major advantage of tetramer technology is the identification of antigen- specific T cells independently of their cytokine secretion profile, ... cells independently of their cytokine-secreting profile Tetramers are also capable of detecting resting antigenspecific T cells, which probably constitute most non-IFN-γsecreting CD8+ T cells of the ... MNCs and of the tetramer-sorted T cell line after weeks of culture In comparison with HLA-B27 tetramer staining, only 68.3% of these antigen- specific T cells were detected by intracellular cytokine...
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Báo cáo y học: "Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease." pps
... successfully to identify antigen- specific CD4+ T cells The intensity of staining correlates with the affinity of TCR for the particular MHC/peptide Although the frequency of antigen- specific CD4+ T cells ... detection of in 1000 Studies have suggested that T cells with these specificities may be present at low frequency in the blood of rheumatoid arthritis patients, and it had been thought that the ... These studies and others [20] suggest that peptide/MHC class II multimers are capable of detecting the great majority of the T cells that can respond to peptide in vitro new construct in each individual...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... factors in the supernatant inhibit the maturation of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric ... vaccine is still not clear in this experimental setting A recent study has demonstrated that vaccination with DCs/tumor fusion cells producing TGF-β resulted in the induction of Treg in vivo and ... which Treg arise in vivo and exert their immunoregulatory effects remain to be defined and are the subject of intensive investigation In the present study, we first show that coculture of T cells...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25