and ledgf p75 binding analysis of in double mutants within loop 170ehlk173
Báo cáo y học: " 3′-coterminal subgenomic RNAs and putative cis-acting elements of Grapevine leafroll-associated virus 3 reveals ‘unique’ features of gene expression strategy in the genus Ampelovirus" potx
... adenylate underlined) in (a) and by an arrow in (b), termination codon of the preceding ORF is underlined and marked with an asterisk and the translation initiation codon is in bold and marked with ... different, with New York and Chile isolates containing 158 nt, and South Africa and Washington isolates having 737 nt In general, the genome of Washington isolate of GLRaV-3, downstream of 5′NTR sequence, ... obtained This strategy, instead of cloning and sequencing by random oligonucleotide primers, decreased the number of steps required for determining the complete genome sequence of the virus and...
- 14
- 353
- 0
Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... comparing band intensity to the Hyperladder I (Bioline) marker Two sets of DNA sequencing primers were designed and validated for each genotype 1a and 1b (table and 2) Each set of primers contains ... alternate samplings of sequences within the viral quasispecies population Record keeping Record keeping and storage of samples and reagents must be meticulous to avoid costly and time-consuming errors ... versus and 6, or lanes and versus and 10) For amplicon 2, AMVRT and M-MLV RT worked equally well (lanes and versus and 8, or lanes and versus and 10) Lanes 11 and 12 are negative controls in which...
- 9
- 444
- 0
báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... comparing band intensity to the Hyperladder I (Bioline) marker Two sets of DNA sequencing primers were designed and validated for each genotype 1a and 1b (table and 2) Each set of primers contains ... alternate samplings of sequences within the viral quasispecies population Record keeping Record keeping and storage of samples and reagents must be meticulous to avoid costly and time-consuming errors ... versus and 6, or lanes and versus and 10) For amplicon 2, AMVRT and M-MLV RT worked equally well (lanes and versus and 8, or lanes and versus and 10) Lanes 11 and 12 are negative controls in which...
- 9
- 442
- 0
Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... bacterial pathogens in joint disease Despite the frequent occurrence of Page of 14 (page number not for citation purposes) genital and intestinal infections in Tunisia [20-24], no studies of ReA-related ... aSemi-quantification of intensity of the 16S rDNA amplification products, visualized using ethidium bromide staining after agarose gel electrophoresis: '+' indicates barely visible band, and '++++' indicates ... synovial samples containing bacterial DNA: 42% of synovial fluid and ST samples in one study [18], and 10% of ST samples in another [17] Our high proportion of bacterial DNA in ST sam- ples from...
- 14
- 500
- 0
Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... positive in 20 of the 27 (74.10%) patients indicating the presence of bacterial 16S rDNA within the synovial samples Of note, PCR was positive in five out of five (100%) patients with ReA and also in ... arthritis fied in three out of seven (43%) RA patients and in three of six (50%) OA patients Accordingly, the presence of bacterial DNA within the SF samples was significantly higher in ReA and UA patients ... risk of triggering an immune response finally culminating in synovitis However, genetic susceptibility factors of the host are also playing an important role particularly in persistent infections...
- 11
- 461
- 0
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells ... in the presence of DTT Fig 12 showed chitin gel before (N1) and after (N2) releasing MCoTI-II Fig 12 Changing in SDS-PAGE protein pattern of chitin gel before and after releasing ReMCoTI-II 1,2: ... system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin ,and proteinases from Plutela...
- 9
- 497
- 0
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers doc
... 100 ng of RNA in a final volume of 10 μl including μl of 10x poly(A) polymerase buffer, 0.1 mM of ATP, μM of RT-primer, 0.1 mM of each deoxynucleotide (dATP, dCTP, dGTP and dTTP), 100 units of MuLV ... another sequence than the miRNA of interest, this will lead to incorporation of the binding site of the TaqMan probe and this unspecific amplicon will be indistinguishable from the desired PCR ... efficiency of miRNA templates than LNA-spiked primers independently of whether intercalating dye or TaqMan probes are used for detection The lower dissociation rates of double- stranded DNA containing...
- 11
- 465
- 0
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization doc
... domains: the larger N-terminal domain (domain I, residues 1±321), which folds into a (b/a)8 barrel, and the smaller C-terminal domain (domain II, residues 322±387), which consists of loops and ... variations in the enthalpy and entropy of conformational changes of importance in binding and catalysis can be due to sequence changes outside the active sites In the evolutionary adaptation of kcat and ... structure of XI are shown in Fig Neither the substrate -binding sites (H53, D56 and K182) nor the metal -binding sites (E180, E216, H219, D244, D254, D256 and D286) were affected by the mutations in the...
- 7
- 268
- 0
báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... specific binding to the relevant tetramer (TIL1520 binding to gp100 and TIL1143 binding to MART-1) Low background binding was observed from cells with no tetramer (0.00% for TIL1520 and 0.02% ... optimal binding and to assure longitudinal assay performance Tetramer binding temperature (room temperature-RT or Page of 25 (page number not for citation purposes) Journal of Translational Medicine ... 2–8°C) was also evaluated and RT was chosen Co-staining with anti-CD3 showed decrease tetramer binding probably due to proximity of CD3 and TCR, therefore anti-CD3 staining was not used Fixed cells...
- 25
- 639
- 0
Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" docx
... TOPO 2.1 Sequence of Randomly Amplified DNA Multi-Cloning Site C Figure PCR Screening and Sequencing of Randomly Amplified Coxsackie Virus A7 cDNA PCR Screening and Sequencing of Randomly Amplified ... Screening and Sequencing of Randomly Amplified Adenovirus Type 17 DNA PCR Screening and Sequencing of Randomly Amplified Adenovirus Type 17 DNA Randomly amplified DNA from Adenovirus 17-infected ... frequency of binding sites within a particular viral genome, but using the EditSeq DNA Editing application (DNAStar), we observed that primers within this mixture will bind every 200–500 bp in both...
- 11
- 387
- 0
Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... concentration of 107 – 101 molecules are shown from left to right (B) Standard curve of GI assay R2 1.00 and a slope of -3.8 was obtained (C) Melting curve of GI standards showing melting point at 90°C in ... combination of both methods greatly speeds up ascertaining when a NoV outbreak is occurring and which strain is responsible A SYBR green mastermix containing a proof reading enzyme would again ... on the left binding to there respective probes Clinical samples binding to probe GII/4 can be seen on the right of both A and B Biotinylated RT-PCR Biotinylated reverse primers G1NVR and G2NVR...
- 8
- 535
- 1
Báo cáo hóa học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" ppt
... TOPO 2.1 Sequence of Randomly Amplified DNA Multi-Cloning Site C Figure PCR Screening and Sequencing of Randomly Amplified Coxsackie Virus A7 cDNA PCR Screening and Sequencing of Randomly Amplified ... Screening and Sequencing of Randomly Amplified Adenovirus Type 17 DNA PCR Screening and Sequencing of Randomly Amplified Adenovirus Type 17 DNA Randomly amplified DNA from Adenovirus 17-infected ... frequency of binding sites within a particular viral genome, but using the EditSeq DNA Editing application (DNAStar), we observed that primers within this mixture will bind every 200–500 bp in both...
- 11
- 347
- 0
Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... concentration of 107 – 101 molecules are shown from left to right (B) Standard curve of GI assay R2 1.00 and a slope of -3.8 was obtained (C) Melting curve of GI standards showing melting point at 90°C in ... combination of both methods greatly speeds up ascertaining when a NoV outbreak is occurring and which strain is responsible A SYBR green mastermix containing a proof reading enzyme would again ... on the left binding to there respective probes Clinical samples binding to probe GII/4 can be seen on the right of both A and B Biotinylated RT-PCR Biotinylated reverse primers G1NVR and G2NVR...
- 8
- 502
- 0
Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses" pdf
... rapidly with in a period of hours compared to hours in two-step typing assays This can provide faster information to the clinicians leading to initiation of suitable symptomatic therapy This single ... collected over a period of six years from India Preparation of RNA standard The detection limit of this assay was determined using RNA standards The RNA standard was produced using T7 transcription ... Emergence and continued circulation of Dengue-2 (Genotype IV) virus strains in northern India J Med Virol 2004, 74:314-22 Gore MM: Need for constant monitoring of dengue infections Ind J Med...
- 5
- 482
- 0
Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
... ces 003 rof C 27 ta noisnetxe lanif dna ces 09 rof C 27 pmet noisnetxe ,ces 54 rof C 06 pmet gnilaenna ,ces 54 rof C 49 ta noitarutaned ,ces 06 rof C 49 ta noitarutaned laitini ,sgniwollof eht ... stibihni dica ciadiakO ]9[ )Mµ 001( niloksrofyxoedid ,1 dna )Mµ 001( niloksrof ,)Mµ 004( BP htiw srh 61 rof sllec amotapeh nekcihc gnitaert retfa dnuof osla saw 73A3PYC fo slevel tpircsnart fo ... saw eneg 1H2PYC dna A3PYC rof oitar noisserpxe evitaleR denimreted saw )adanaC ,satnemreF( reluR eneG reddal AND bK dradnatS ot ecnerefer htiw nitca-β dna 1H2PYC ,A3PYC rof ytitnauq fo noitatitnauq...
- 7
- 291
- 0
Báo cáo y học: " Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt" potx
... Organization of Animal Health (OIE) The assay was initially highly successful in detecting H5N1 infections in Egypt [5,7] Since 2007, however, an increasing number of strains in Egypt escaping detection ... actions of rapid and specific testing, culling and vaccination of poultry is the key to mitigate the risk of human infections and fatalities in Egypt Controlling the endemic HPAIV H5N1 situation in ... of a multiplex RTqPCR assay for detection and differentiation of Egyptian H5N1 HPAI viruses of clade 2.2.1 proper (p) and the emerging 2.2.1 lineage of viruses which escape standard vaccine-induced...
- 8
- 295
- 0
báo cáo khoa học: " Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data" potx
... experimental set by Miner software [29], and the values were used in all subsequent analysis (Table and Additional file 2) Miner software pinpoints the starting and ending points of PCR exponential ... expression analysis is increasingly important in many fields of biological research Understanding patterns of expressed genes is crucial to provide insights into complex regulatory networks and will ... available for most of plant species, including cotton The classical housekeeping genes involved in basic cellular processes such as 18 S rRNA, ubiquitin, actin, b-tubulin, and glyceraldehyde-3-phosphate...
- 12
- 390
- 0
Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
... comprising Coronaviridae and Arteriviridae Archive of Virology 1997, 142:629-633 Hill H: Overview and history of mystery swine disease (swine infertility and respiratory syndrome) Proceedings of ... Mystery Swine Disease Communication Meeting Denver, CO 1990:29-31 Polson DD, Marsh WE, Dial GD: Financial evaluation and decision making in the swine breeding herd Veterinary Clinics of North ... serially to 10-7 and were assayed in a 25 μl reaction mixture containing μl of diluted RNA; 0.4 μM of Nf primer and 0.4 μM of Nr primer; 12.5 μl of 2× One Step SYBR RT-PCR Buffer; μl of PrimeScript™...
- 7
- 350
- 0
Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
... nucleotide sequence of the CP gene and the amino acid sequence of coat protein showed that all PVM isolates fell into two distinct groups - I and II (Table and Fig 3) PVM isolates in group I only ... China, Poland and Russia and group II consisted of Canadian and US isolates Isolates in group I might be further divided into two sub-groups - Ia, and Ib PVM isolates from China and Poland formed ... primers PVM3 and PVM4, a NcoI site was revealed in PVM isolates detected in Germany, Italy, Russia, Poland and China, but not found in the isolates detected in the US (Idaho strain) and Canada...
- 7
- 452
- 0
Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... 5), indicating a high specificity for influenza A viruses of subtype H5 Evaluation of sensitivity and specificity in clinical specimens The sensitivity of the assay was clinically evaluated in ... bases were incorporated into the forward and reverse primers, respectively As the binding efficiency of the original TaqMan probe was inadequate, LNA residues were incorporated that result in a tighter ... detection of clade and H5N1 viruses in a large number of clinical specimens (n = 58) The assay described here has been established within the laboratories of the South East Asia Infectious Disease Clinical...
- 5
- 460
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25