analysis of intrinsically disordered proteins by small angle x ray scattering
Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
... Determination of binding constants from forward scattering The intensity of Ôforward scattering , or I(0), is the X- ray solution scattering that is parallel to the incident beam, and was determined by extrapolation ... prior estimation of the maximal dimension The forward scattering is proportional to the product of the molecular mass and concentration of the scattering particle For a mixture of particles, the ... molecular mass of the scattering species The forward scattering values for various concentrations of wild-type arrestin were measured by extrapolation of Guinier curves [21] to an S value of qffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi1...
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Báo cáo y học: "Application of Small Angle X-ray Scattering (SAXS) for Differentiation between Normal and Cancerous Breast Tissue"
... Evaluation of optimum scattering angle - In order to obtain the optimum scattering angle, the diffraction profiles of a test adipose sample were measured over a range of scattering angles from 4° to ... Elshemey WM Low angle X- ray scattering from spices Radiation Physics and Chemistry 2002; 64: 267-271 Evans SH, Bradley DA, Dance DR, Bateman JE, Jones CH Measurement of small angle photon scattering ... diagnosis using scattered X- rays J Synchrotron Rad 2000; 7: 348-352 Malden CH, Speller RD, et al A CdZnTe array for the detection of explosives in baggage by energy dispersive X- ray diffraction signatures...
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Báo cáo y học: "Insights into the regulation of intrinsically disordered proteins in the human proteome by analyzing sequence and gene expression data" potx
... Edwards et al R50.8 Figure A summary of expression profiles for the highly disordered proteins A summary of expression profiles for the highly disordered proteins (a) The heat map displays four ... disordered transcripts Log10 of the number of tissues in which the transcript is expressed (x- axis); log10 expression of the average magnitude of expression within each tissue (y-axis) The points have ... accumulation of disordered proteins and their transcripts in the cell Unexpectedly, for a proportion of highly disordered proteins, all four of these trends were reversed The highly disordered proteins...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... 0.2 min Fig Time-lapse LSM analysis of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC ... suggesting the existence of some interactive cellular partner FRAP analysis of the nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part of the single compartment of nuage was ... properties of proteins in the PGC determined by FCS and FRAP A fusion protein consisting of Olvas and green uorescent protein (GFP) (OlvasGFP) expressed in the PGC forms granules that exhibit an...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... inclusions and oxidation Comparative analysis of the protective effect of NAC and Hsps against ROS, protein oxidation, iron and nitric oxide level upregulation caused by expanded httEx1 expression ... generated by httEx1-103Q-EGFP expression 3084 Analysis of httEx1-polyQ expression with regard to the three major proteolytic activities of 20S proteasome, a phenomenon partially restored by Hsp expression ... Huntingtin inclusions and oxidation W J J Firdaus et al Fig httEx1-polyQ expression enhances reactive oxygen species (ROS) levels (A) Analysis of ROS induced by httEx1-polyQ expression COS-7 cells...
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IDENTIFICATION OF INTRINSICALLY DISORDERED SEPTAL PORE ASSOCIATED PROTEINS REGULATING DIVERSE ASPECTS OF
... composed of two connexons or hemichannels, with each cell contributing one Each connexon is a hexamer of connexin, a four transmembrane protein A connexon can be composed of a single type of connexin ... IDENTIFICATION OF SEPTAL PORE ASSOCIATED PROTEINS 4.1 Results 4.1.1 Mass Spectrometry Identification of SPA proteins .51 4.1.2 Machine Learning Identifies the SPA Family of Intrinsically Disordered Proteins ... SPA proteins possess features of intrinsically disordered proteins 69 Figure 12 Disorder, coiled-coil, and sequence homology of SPA proteins 71 Figure 13 Biochemical analysis...
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Structural basis for recruitment of traffic proteins by small GTPases
... 1982) The G3 box, DXXGQ, is responsible for the binding of the γ-phosphate of GTP and Mg2+ The guanine nucleotide ring is partly recognized by the G4 box, NKXD, and by the G5 box, (T/G)(C/S)A ... the tetrapeptide motif Rab proteins terminate in a distinct set of cysteinecontaining C-terminal motifs (CC, CXC, CCX, CCXX, or CCXXX) that are similarly 18 19 modified by geranylgeranyltransferase ... part of this introduction will focus on the GTP-binding proteins (G proteins) and more specifically on the small GTP-binding proteins, Ras superfamily 1.2 GTP-binding proteins (G proteins) G proteins...
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A discourse analysis of english speeches delivered by guest speakers at graduation ceremonies in US colleges and universities
... THEORETICAL BACKGROUND 2.2.1 Text 2.2.1.1 Concept of Text 2.2.1.2 Features of Text 2.2.2 Discourse & Discourse Analysis 2.2.2.1 Concept of Discourse 2.2.2.2 Concept of Discourse Analysis 2.2.3 Written ... The focus of the study is on the discourse analysis of EGSs so the concept of discourse analysis, text, cohesion, and coherence are included in this chapter I have chosen the viewpoints of some ... confined to the investigation of some discourse features of graduation speeches The scope of the study is just limited to the analysis of formal speeches delivered by guest speakers at graduation...
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Tài liệu A Comparative Analysis of Individual Communication Processes in Small docx
... them in exchange for unquestioning loyalty (Hofstede, 1991, p.260) Although the contrast of cultural I-C has been used to explain and organize a wide variety of behaviors in a number of different ... defined by (a) the length of speaking time by each group member and (b) the number of turns taken by individuals To assess turn-taking communication behavior, the turn-taking framework developed by ... number of turns taken by each participant was codified, counted, and compared Speaking time was calculated by using a stopwatch to measure the exact length of each conversational turn The length of...
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An analysis of errors commonly committed by high school students in using english questions
... For example: He likes drinking coffee Does he like drinking coffee? Figure 1.4: Classification of Yes – No questions by Vo (2000) 43 primary primary auxiliaries auxiliaries Y/N with Aux as ... “th” of “mother” as “th” of “three”; or “dZ” of “fridge” as “r” of “rain” At morphological level, Vietnamese learners tend to omit the plural suffixes at the end of the word as plural form of word ... breadth of context which the analyst must examine, and extent, the breadth of the utterance which must be changed in order to fix the error Errors may also be classified according to the level of...
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An analysis of the inaugural address by g w bush in the u s president election 2004 from a perspective of discoure analysis
... world at large: the context. Context of culture Context of situation TEXT Figure 1.1: Theory of context of situation Cook, in the same study of language in and out of context, adds that when we ... different types of context Linguistic context: the language that surrounds or accompanies the piece of discourse under analysis Non-linguistic context (experiential context): type of communicative ... of language but the interpretation of that use needs non-linguistic features of the discourse 1.1.3.3 Features of context The best known overview of the context of situation is perhaps that of...
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Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx
... result of selection If the cutting sites are in the flexible loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins studied by ... suggested that proteins occurring in nature have been evolved by the assembly of nonhomologue genes For small protein domains, they may have evolved by assembly and/or exchange of small gene segments, ... at 12 surface-exposed positions In their study, six of these positions were randomized by saturation mutagenesis, in which any of the 20 amino acids can occur at each of the six positions Selection...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... eliminate excessive viscosity of the extract as a result of DNA, lL of DNase I (200 units) was added per 100 lL of buffer [30] Following filtration through two layers of miracloth and the addition of ... overestimate the presence of soluble proteins and underestimate the expression of membrane-associated proteins Despite these problems, the proteomics analysis of the protein complement of normal mouse ... studies that have shown a persistent expression of calsequestrin in mdx microsomes [29], the analysis of total muscle extracts clearly showed a reduction of this luminal constituent in dystrophic...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... pET28a– a4D222 pET–TIPRL pProEx–PP2Aca pGEX) 5X2 pGEX) 5X2 –PP2Aca pGEX) 5X2 –PP2Aca pGEX) 5X2 –PP4c (175–307) pGEX) 5X2 –PP6c (106–305) pGEX) 5X2 –PP2AcCT (210–309) pET–GST–TIPRL exADBD::TIPRL TRP1 lm GAL4AD::PP2Aca ... were coexpressed with His–TIPRL in E coli GST fusion proteins were isolated from extracts by binding to glutathione–Sepharose beads Bound proteins were resolved by SDS ⁄ PAGE and detected by immunoblotting ... the reciprocal experiment, binding of His-a4 to the previously formed GST– PP2Aca:His–TIPRL complex was also observed (data not shown) Because of the lower levels of expression of GST–PP2Aca relative...
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Báo cáo khoa học: Phosphorylation of NF-jB proteins by cyclic GMP-dependent kinase A noncanonical pathway to NF-jB activation potx
... chosen concentrations of reporter DNA, PKG induces a less than twofold induction of transactivation by endogenous p65) Expression of the transfected proteins was confirmed by Western blotting All ... the larger and smaller NF-jB complexes were induced by the conventional antigen In contrast, predominantly the smaller complex, represented by the lower band on the gel, was induced by stimulation ... the lack of transactivation is not overcome by cotransfection of PKG (Fig 2B) We noted that the transfection of PKG alone was sufficient to increase the consensus reporter activity by approximately...
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Báo cáo khoa học: High-pressure effects on horse heart metmyoglobin studied by small-angle neutron scattering pdf
... Guinier, A & Fournet, G (1955) Small- Angle Scattering of X- Rays J Wiley & Sons, New York, USA Ibel, K & Stuhrmann, H.B (1975) Comparison of neutron and X- ray scattering of dilute myoglobin solutions ... following analysis of the scattering data the actual protein contrast given by Eqn has been replaced by the quantity ! NA K0 Pị ẳ bp q0 b Pịvp 0:1ị 4ị Mp where q0b P ị is given by Eqn Using this expression ... the reorganization of the secondary structure of Mb that has been observed by FTIR [23,24] does not affect the compactness of the protein According to Eqn the slope of the plot of c(P) [K0(P)]2/...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... subunits in Western blot of crude extracts separated by SDS/PAGE Lane 1, crude extract of P putida KT2440; lane 2, crude extract of P putida KT2440 pJB653; lanes 3–5, crude extracts of different P putida ... microwave power of mW Trace B is multiplied by a factor of six to show the small signals of the rapid species in this sample The arrows indicate the position of contribution of the resting species ... Comparative EPR and redox ¨ studies of three prokaryotic enzymes of the xanthine oxidase family: quinoline 2-oxidoreductase, quinaldine 4-oxidase, and isoquinoline 1-oxidoreductase Biochemistry...
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Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... sequence database for proteins that are likely to be new members of a functionally related family of membrane proteins The program runs on a personal computer, and the analysis of an organism’s total ... environment that is built into AxoGraph (Axon Instruments, CA), a scientific data analysis and graphics program for Macintosh computers (http://www.axon.com/CN_AxoGraph4.html) The AxoGraph plug-in programs ... in AxoGraph Features common to all of the profiles were identified by eye AxoGraph’s convenient browsing features aided in this task Every profile had a cluster of three peaks located approximately...
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analysis of the astronomers wife by kay boyle
... between the two typesof people is necessary, whether either one realizes it, for the world to function The "Astronomer's Wife" is an excellent short story that brings out the often forgottenpoint ... Ames perhaps could never get along without her husband, itwas no fault of her own that she didn't She provided a comfortable existance for the astronomer so that he would be free to his work, and ... her lifestyle avalid one Of course an understanding was never reached, because otherwise the authorwould not have been able to illustrate the similar conflicts that exist in today's societyso...
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analysis of cuo nanoparticles synthesized by sol gel
... deformation of lattice due to strain caused by imperfection and inelastic scattering of charge carriers by phonons[16-18] The absorbance of CuO sample synthesized with propanol solvent shows faster exponential ... using propanol as solvent The shifting of XRD peaks to higher angle may be a consequence of stain effect Table Evolution of crystallite size and strain of CuO nanoparticles synthesized with different ... Microstructure and Optical Absorption Analysis of CuO Nanoparticles Synthesized by Sol-Gel Route served XRD pattern Our XRD results thus confirm synthesis of pure and well crystalline CuO nanoparticles...
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Tìm thêm: xác định các nguyên tắc biên soạn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25