an in vitro study
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... membrane[36] and also the loading procedure does not affect the MCV This finding is in agreement with previous report[34] Non-significant change in both MCH and MCHC can be explained by pravastatin ... including vinblastine, chlorpromazine, hydrocortisone, propranolol, tetracaine, retinol, and primaquine [16, 19] The current work aims to study the encapsulation of pravastatin in human carrier ... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence...
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báo cáo hóa học:" Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study" docx
... type II and aggrecan core protein was steadily expressed in fibrin/PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/PLGA and PLGA at weeks and weeks β-actin gene ... design of the study and conceived of the study GK participated in the design of the study, conceived the study and drafted the manuscript All authors read and approved the final manuscript 13 ... implantation in a canine model: change in composition of reparative tissue with time J Orthop Res 2001, 19:482-492 Munirah S, Samsudin OC, Chen HC, Hussein SSS, Mohd Azam Khan GK, Aminuddin BS,...
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báo cáo hóa học:" The effect of newer anti-rheumatic drugs on osteogenic cell proliferation: an in-vitro study" doc
... guidelines has been established in some reviews[17,18] In light of these many uncertainties, we carried out an invitro study of the effect of various antirheumatoid drugs alone and in combination ... Scotland) to remove cells remaining in the intra-trabecular spaces and plated at fragment per well in a 24-well plate containing ml of control medium (α-MEM containing 1% Gentamicin v/v and 10% ... increasing the action of the first and negating that of the second That drugs can interact is not unexpected Systematically investigating such interactions in vitro is slowly becoming a routine...
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Báo cáo khoa học: "Contribution of the xenograft bone plate-screw system in lumbar transpedicular stabilization of dogs: an in-vitro study" docx
... than group I In TS, the usage of the MPS system has some disadvantage such as loosening, bending, breaking or pulling out of the screws and plates, and the maximum inter- segmental rigidity and ... Harper KD, Nightingale RW The role of imaging and In Situ biomechanical testing in assessing pedicle screw pull-out strength Spine 1996, 21, 1962-1968 Sandén B, Olerud C, Petrén-Mallmin M, Larsson ... calculated in Fig Calculation of radian angles for converting the forces to the moments Fig The results of the Euclidian distance method and the four main clusters two steps: First, the stiffness in...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... immunofluorescence and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected ... tissue In the present article we established an in vitro model system to investigate the survival and phenotype of human MSCs following injection into bovine NP tissue explants to test the following ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer...
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Báo cáo y học: "Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study" pptx
... quiescence, and induces apoptosis in HT-29 colon adenocarcinoma cells J Clin Invest 1995, 96:491-503 47 Zhang X, Morham SG, Langenbach R, Young DA: Malignant transformation and antineoplastic ... important impact on long-term out- come Therefore it remains important to extend these in vitro studies with animal in vivo studies and even clinical setups Conclusion Although they are in vitro findings, ... sulphate incorporation rate Also, naproxen exhibited adverse effects in an in vivo study using the ACLT canine model of OA [16] That study found that naproxen increased water content in cartilage...
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Báo cáo y học: "Exogenous sphingomyelinase increases collagen and sulphated glycosaminoglycan production by primary articular chondrocytes: an in vitro study" pps
... mean ± strandard error * P < 0.05 2AP, 2-aminopurine; cpm, counts/ min; PKR, protein kinase R; sGAG, sulphated glycosaminoglycan tive mRNA splicing, namely types IIA and IIB, which include and ... This in accordance with studies in human keratinocytes, which have shown that a rapid (15 minutes) but transient (returning to baseline by hour) increase in endogenous ceramide occurs following ... collagen-glycosaminoglycan interactions J Biol Chem 2001, 276:5303-5309 Chua KH, Aminuddin BS, Fuzina NH, Ruszymah BH: Insulintransferrin-selenium prevent human chondrocyte dedifferentiation and promote...
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Báo cáo y học: "Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an in vitro study" docx
... TH and JR participated in gene expression data processing and in study design and coordination CK and MS conceived the study and participated in its design and coordination All authors read and ... several genes involved in the NF-κB signaling pathway showed differential expression, including interleukin-1 receptor antagonist (IL1RN), receptor-interacting serine/threonine kinase (RIPK2), ... processing, participated in the design and coordination of the study and drafted the manuscript KA, LM and TD conducted the cell culture experiments and performed the protein membrane arrays and...
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Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
... modulating their expression or intrinsic adhesive functions [18] PMNs maintain several of these receptors in an inactive state during transit in the bloodstream and extracellular fluids, and only ... Martini Hospital, Turin, for providing human umbilical cords, and the Blood Bank, Molinette Hospital, Turin, for providing human blood References 10 11 12 13 14 15 Vane JR, Bakhle YS, Botting ... adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro J Clin Invest 1984, 74:1581-1592 Zimmerman GA, McIntire TM, Prescott SM: Thrombin stimulates...
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Báo cáo y học: " Sodium nitroprusside and peroxynitrite effect on hepatic DNases: an in vitro and in vivo study" potx
... 8-nitroguanine can be depurinated yielding apurinic sites capable of inducing GC→TA transversions, GC→CG transversions and deletions [17,36] The appearance of the nitroguanine peak during the scan analysis ... analysis and assisted during in vivo experiments DP and RK assisted during in vitro experiments and participated in the design of the study All the authors read and approved the final manuscript ... continuously produced in humans and animals It can be derived from L-arginine through the enzyme nitric oxide synthase (NOS) and by different NO donors, including SNP NO has been shown to play an...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... intraflagellar transport also Tg737, intraflagellar transport 88 intraflagellar transport protein A intraflagellar transport protein B immunohistochemical staining interleukins interleukins 13 interleukin ... remodeling, and stromal edema Epithelium abnormalities in NP involved with stem cells and their progenitors might result from intrinsic (including epigenetic) alterations in their transcriptional and ... epithelium in NP paraffin sections A comparison of the in vivo and in vitro results indicated that this cell model could simulate the in vivo pathophysiological changes of NP in vitro In conclusion,...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
... expression in fibroblasts and hRE Results Human skin cells GSM-900 signal did not induce apoptosis or affect HSP expression in normal human epidermal keratinocytes As shown in Fig 1A, in normal human ... mJÆcm2 single dose, h post exposure) Scale bar: 25 lm Human reconstructed epidermis GSM-900 did not induce an in ammatory process in hRE In these experiments using haematoxylin ⁄ eosin-stained reconstructed ... expression and induce DNA damage in a fibroblastic cell line and human fibroblast primary cells, respectively [58,59] In our study, the effects of GSM-900 exposure on human skin cells were investigated...
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Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx
... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... grown in Eagle's minimal essential medium containing 5% fetal calf serum (FCS) (Sigma, St Louis, MO), mM glutamine (Invitrogen, Carlsbad, CA), and 15 µg/ml gentamicin sulfate (Invitrogen) in a...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
... as Fe and Cu [32-34]; other groups emphasize that organic and black carbon components found mainly in ultra-fine particles confer greater in vivo and in vitro toxicity than fine particles, and ... (ROS) induce chemical and structural changes on human insulin in vitro, including alterations in its immunoreactivity Frontiers in bioscience 2005, 10:838-843 Olivares-Corichi IM, Ceballos G, Medina-Santillán ... the fine fraction indicates a combination of natural and anthropogenic sources influenced by smelter and incineration emissions in the study area Table 3: SEM classification of individual PM10particles...
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báo cáo hóa học:" Development of an in vitro three dimensional loading-measurement system for long bone fixation under multiple loading conditions: a technical description" potx
... longitudinal-circumferential shear modulus G, with published machine testing determined values ranging from 3300 to 5000 (N/mm2) for human and bovine femur and tibia [17] Thus use of E = 18,000 and ... ostectomized-instrumented equine radii It is the authors' opinion that the 3D loading-measuring system described in this manuscript is capable of creating "worst case" clinically relevant loading conditions, ... lateral and cranial (anterior) radius surfaces with at least a 0.5 cm distance from potting material, and to have sufficient bone-potting interface to transmit loads to failure of the instrumented...
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báo cáo hóa học:" Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" docx
... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... grown in Eagle's minimal essential medium containing 5% fetal calf serum (FCS) (Sigma, St Louis, MO), mM glutamine (Invitrogen, Carlsbad, CA), and 15 µg/ml gentamicin sulfate (Invitrogen) in a...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... activity, including phagocytosis, respiratory burst, and transendothelial and transepithelial chemotaxis induced by arginine-glycine-aspartic acid (RGD) containing synthetic peptides and protein [17] ... of α5β1 integrin and CD47/IAP [41] It has been speculated that the five membrane-spanning segments of CD47/IAP and the two membrane-spanning domains of the heterodimeric integrin form an ad hoc ... assessed by measuring either changes in membrane potential, changes in protein tyrosine phosphorylation, or changes in the relative levels of aggrecan mRNA within cells Available online http://arthritis-research.com/content/10/1/R4...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pptx
... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... can depend on the type and intensity of the stimulus, and vary according to the considered cytokine LPS induced similar levels of IL1 production in males and females, with levels increasing in ... and NL participated in the design of the study GC and JD conceived of the study, and participated in its design and coordination All authors read and approved the final manuscript Author Details...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pot
... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... can depend on the type and intensity of the stimulus, and vary according to the considered cytokine LPS induced similar levels of IL1 production in males and females, with levels increasing in ... and NL participated in the design of the study GC and JD conceived of the study, and participated in its design and coordination All authors read and approved the final manuscript Author Details...
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Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx
... immunoprophylaxis in patients with neoplastic deseases II Estimation of antihaemagglutinin and antineuraminidase antibody titre or influenza viruses A and B Int Rev Allergol Clin Immunol 1999, ... streptomycin) Cells were incubated in serum-free MEM containing μg/ml trypsine, mM L-glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin Determination of virus titre The respective cells were incubated ... viruses were produced and inactivated with an UV-GS linker The virus titre inducing 20-30% IFN synthesis (calculated with an IFN-a standard) was determined in serial dilutions and kinetic experiments...
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