alpha proton detection based backbone assignment of intrinsically disordered proteins
STRUCTURE ANALYSIS OF POLYSACCHARIDE ESTERS
... from the ratio of the spectral integrals of the protons of the RU and the methyl protons of the acetyl moiety Alternatively, trimethylsilylation of cellulose acetate with a wide range of DS values ... these protons A H,1 H-COSY NMR spectrum of this region is displayed in Fig 8.12, confirming the assignment Consequently, the spectral integrals of the protons of the AGU and the methyl protons of ... ratio of the spectral integrals of the AGU protons and the methyl protons of the acetyl moiety This is also useful for the DS determination of long-chain esters via the spectral integrals of the...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant avoredoxin Reduction titration of avoredoxin by NAD(P)H The reduction of ... Figure shows the results of linear regression analysis of the logarithms for the redox ratio of the mediator versus that of recombinant avoredoxin The redox potential of oxidized avoredoxin reduced ... His126 of A fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivative, although a ferric ion was not found at the site of either...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... exchanging properties of the amide protons of SGCI[L30R, K31M] are very similar to that of SGCI Both the amide proton of Lys13 of SGCI and of SGCI[L30R, K31M] variant and Asp13 of SGTI are quicklyexchanging, ... Fig Backbone of 10 superimposed structures of SGCI (A) and 10 superimposed structures of SGTI (B) Schematic representation of the secondary structure elements and the disul®de bonding pattern of ... peaks between residues of the binding loop and that of the 11±16 loop are also different from that of SGCI and its variant (Table 3) Qualitative evaluation of H±D exchange data Proton deuterium exchange...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... identity of the peptide, and hence the presence of des-(27–31)C-peptide in extracts of mouse pancreatic islets Two other signals of importance were detected whose masses agreed with those of glucagon ... signals of b- and y- fragments of both C-peptides and the truncated form of C-peptide from proinsulin (Fig 4) were detected in the LC-nozzleskimmer experiments The signal from the truncated form of ... experiment, a spectrum of 200 scans, 512 K data points was collected The spectrum was calibrated using a dataset of a sample of standard peptides After calibration, the masses of the standard peptides...
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báo cáo hóa học:" Growth and structure analysis of tungsten oxide nanorods using environmental TEM" doc
... the wire and the behavior of the interface between the nanorods and wire due to the difficulty of the transmission of electrons for TEM analyses In this case, a part of the tungsten wire was fabricated ... intervals of 0.38 nm in the longer directional axis of the nanorods and in intervals of 0.37 nm in the perpendicular direction to the longer directional axis These interval distances of 0.38 and ... image (b), and SAED pattern (c) of the tungsten oxide nanorod Figure TEM of tungsten nanowire and HRTEM and TEM images of the nanorod (a) TEM image around the surface of non-heated tungsten wire...
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Báo cáo y học: "Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation" pot
... different proteins from one gene [17,18] For instance, a brainspecific isoform of NFI-A [3], which was first isolated in 1990 by Inoue and coworkers [19], activates the transcription of mouse ... functions based on Gene Ontology (GO) Biological Process categories (Additional data file provides a list of assignments) Among the 197 upregulated genes, a large percentage (34.6%) of the candidate ... that encode RNA binding proteins, transcription factors and ribosomal proteins, and comprise only 10.4% of probe sets on the microarray Figures and show the distribution of gene functions in the...
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Crystal structure analysis of pils, a type lvb pilin from salmonella typhi
... pilin structure 83 Figure 4-16 Model of the structure based TCP model 84 Figure 4-17 A close up view of the neighboring subunits of structure based model of TCP (PDB code:1or9) with ∆PilS crystal ... Superposition of the complex structure and the native structure in the vicinity of the peptide 77 The surface charge property of the native ∆PilS molecule and the complex 78 Superimposition of the backbones ... (in preparation) iii TABLE OF CONTENTS Page Acknowledgements i Publication iii Table of contents iv Summary viii List of abbreviations ix List of figures xii List of tables xiv CHAPTER MACROMOLECULAR...
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A study on the structure of the speech “ I have a dream” by Martin Luther King A systemic functional grammar analysis
... facilitating communication in all modes, not as an isolated area of study” (G Lock, 1996) As having the experience of drilling with a number of grammar exercises in schooling as well as being the teacher ... (1996), Van Hoang Van (2006)… The content of the speech “I have a dream” is retrieved from the official and popular website www.americanrhetoric.com 1.6 Design of the study There are four main chapters ... Martin Luther King - a systemic functional grammar analysis based on Halliday’s functional grammar as the theoretical framework 1.2 Aims of the study In carrying out the research, the writer aims...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly ... steady-state microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, ... rates of NH4+, NO2-, and NO3- The biofilm surface was at a depth of zero The values are means of triplicate measurements Microelectrode Measurements Steady-state concentration profiles of O2,...
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An Analysis of Domestic Water Demand Structure and Water Recycling in Beijing
... center of Beijing Fig - Location map of Beijing city and Haidian District center, and has many places of interest and famous shopping areas (Beijing Municipal Bureau of Statistics, 2005b) One of ... (L/year) NBd: number of households of size d in Beijing City (number) The composition of households in Beijing City was based on a statistical yearbook (Beijing Municipal Bureau of Statistics, 2005b) ... Beijing City So, the results obtained here can be thought of as being typical of Beijing City By the end of 2003, the registered population of the district was 2.4 million, 3.4% more than in 2002...
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an economic analysis of financial structure
... Financial Intermediation Asymmetric Information Conflicts of Interest • Conflicts of Interest is a type of moral hazard problem caused by economies of scope • Arise when an institution has multiple objectives ... impermissible audit Conflicts of Interest: Remedies II • Sarbanes-Oxley Act of 2002 (cont’d) – Beefs up criminal charges for white-collar crime and obstruction of official investigations – Requires ... Conflicts of Interest: Remedies I • Sarbanes-Oxley Act of 2002 (Public Accounting Return and Investor Protection Act) – Increases supervisory oversight to monitor and prevent conflicts of interest...
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02 three dimensional static and dynamic analysis of structure
... group (or patch) of elements, of arbitrary shape, is subjected to node displacements associated with constant strain; and the results of a finite element analysis of the patch of elements yield ... the equations of statics or the equations of geometry, in general, we should satisfy the equations of statics for the reasons previously stated However, because of the existence of inexpensive ... effective for the development of structural analysis programs The static and dynamic analysis of structures has been automated to a large degree because of the existence of inexpensive personal computers...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... chromatin biology In this area, ETD -based MS has enabled detection and site-mapping of combinatorial modifications on highly basic histone proteins [14] Two copies of each of the four core histones, H2A, ... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser ... versions of the L34 protein are present in a ratio of : 20 Analysis of the spectra recorded on the other peaks in the base peak ion chromatogram allowed us to detect and identify 46 of the 55 proteins...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... superposition of the exponential rise to analyze quantitatively the contribution of each phase The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... thylakoids in the absence of additives or in the presence of lm DCMU or dPQ over a range of temperature from 15 C to 25 C The range of temperature was set on the basis of the membrane transition ... Boisvert et al have attributed both of the two latter phases to the reduction of the acceptor side of PSII [9,12,13], or more specically to the reduction of two distinct plastoquinone (PQ) pools...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... leads to formation of the active complex, whereas binding of a minimum of m Mn2+ ions forms the inactive complex The sizes of the active and inactive fractions are the result of competitive metal ... presence of Mn2+ The midpoint of the Mg2+ titration curve in the absence of Mn2+ is approximately 4.5 mm, and nearly full activity is reached by 10 mm Mg2+ Hill analysis of the data gives n-values of ... parts of P7, J8 and P9.0 protected The Cr.LSU pattern is distinct in that the rst three nucleotides of P7, and the 3Â-half of J8 7, are not protected The rst three nucleotides of P7 are part of...
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Tài liệu Báo cáo khoa học: "Identifying Linguistic Structure in a Quantitative Analysis of Dialect Pronunciation" docx
... 3.2 Measuring of Dialect Distances Aggregate analysis of Bulgarian dialects done in this project was based on the phonetic distances between the various pronunciations of a set of words No morphological, ... representative The method of quantification of regular sound correspondences described in the second part of the paper was successful in the identification of the underlying linguistic structure of the dialect ... analysis based on a partition of a set of objects into groups or clusters (Manning and Schütze, 1999) Multidimensional scaling is data analysis technique that provides a spatial display of the...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... in Fig 2A From 2.5 g of serum proteins, mg of homogeneous DM64 were obtained Physicochemical and chemical characterization DM64 has a molecular mass of 63 659 Da by MALDITOF MS SDS/PAGE, under ... respectively, both of which are homodimeric proteins in native conditions The precise mode of action of class II PLA2 myotoxins remains elusive However, it seems clear that the initial target of these ... comparison of the first three domains of DM64 and a1Bglycoprotein shows that they are homologous to the three DM43 domains [24] Each of these domains in the three proteins possesses typical signatures of...
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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... levels of laminin c1 chains in EDTA/detergent extracts of various mouse tissues The content of b1 chains in these extracts exceeded the content of b2 by a factor of 5±20 The relative amounts of b2 ... density of synaptic junctions (usually accounting for less than 0.1% of the total basement membranes) The radioimmunoassays also indicated a twofold higher content of b1 chain in lung and kidney of ... mean (SD) length of the side chain was estimated from electron micrographs of 30 particles to be 40 ( 10 nm), which is in good agreement with a molecular mass of 20 kDa Mutation of Ser721 to Ala...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference Proton ... build-up of an interesting proton pair with that of a reference proton pair which has a known fixed distance, i.e geminal protons PDTRP offers three well resolved reference points, each of which ... of geminal protons ˚ with a proton/ proton distance of 1.8 A: Asp2-b/b¢, Pro5-d/d¢ and the C-terminal carboxamide NH protons As can be seen from the three panels in Fig 4, the initial slopes of...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... [13], yellow), based on domains I and II The arrow indicates the direction of projection to the circle in (C) (B) As (A), view 90° away (C) Comparison of the positions of the tip of domain IV in ... 2010 FEBS 3791 Crystal structure of Staphylococcus aureus EF-G A Y Chen et al B C 38 Å Fig Conformational space of EF-G on and off the ribosome (A) Superposition of S aureus EF-G with the ribosome-bound ... domain III, which is disordered in structures of wild-type T thermophilus EF-G [12,13] In molecule B, part of the switch I region could be built The entire switch I region is disordered in all previous...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25