ais block the enzyme aromatase that is responsible for the peripheral conversion of
Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme Biochem ... sensitivity of the human enzyme, and showed that the variant, Glu17, in the pig enzyme is responsible for its peculiar kinetic characteristics (low carnitne Km and high malonyl-CoA IC50) This is the ... related to the low IC50 of this recombinant enzyme [26] As the pig N-terminal fragment is able to change the malonyl-CoA sensitivity of the human enzyme (Fig 2C), we propose that the pig enzyme can...
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Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf
... novo synthesis, it might be involved in the stearic acid incorporation after the de novo synthesis of this lipid (i.e the second hypothesis) According to this hypothesis, previously raised for mammals ... in the de novo synthesis of a given phospholipid, including PI Hence, the rate of synthesis and the amount of CDP-DAG molecules that were used as substrate for PI synthesis appeared to be the ... yeast The first hypothesis, previously raised for plant cells [33], involves the synthesis of two kinds of CDP-DAG molecules: the first type containing stearic acid at the sn-1 position would be the...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... specicity of the substrate) [26] Consequently, substitution of Val371 to isoleucine might prevent the full development of the active conformation This hypothesis is further conrmed by the results of ... in this position most likely causes some degree of structural change; this is especially true in the case of the introduction of an isoleucine, a b-branched amino acid that is not exible In the ... are consistent with the FXI:C and FXI:Ag measured in the patients plasma, further supporting the hypothesis that the Val371Ile mutation is a 6130 type II defect leading to the production of a defective...
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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
... whether djmj is a functional homolog of mammalian jmj, we tested the chromosomal distribution of mJmj and its ability to rescue the phenotypes of the djmj mutants To this end, transgenic flies that ... strains that contain the P or piggyBac transposons in the djmj gene locus were identified The djmjEY02717 allele is an insertion of the EY element [28] in the 5¢-UTR of djmj However, this insertion ... bristles of the progeny were scored for Sb expression On the wild-type genetic background, 29.7% of bristles showed the Sb phenotype As a positive control, we confirmed that on the background of FEBS...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
... of CIIIC on the proteolysis of r32 by HflB was also assayed in the presence of CIIIC (60 lm) instead of CIII (Fig 2B) It is clear that in this A B C Fig ATP-dependence of proteolysis of r32 by HflB ... proteolysis with GST–HflB (0.8 lM) for 40 in the presence of His6–CIII or CIIIC (up to 200 lM) are shown in the upper panels Numbers on the top of each lane indicate the concentration of His6-CIII ... itself is a substrate of HflB [12] CIIIC, the central region of CIII, is not a substrate of HflB, and acts as a better inhibitor for digestion of CII by HflB, than CIII [11] Is this mode of antiproteolytic...
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Báo cáo khoa học: Redox reaction between amino-(3,4-dihydroxyphenyl)methyl phosphonic acid and dopaquinone is responsible for the apparent inhibitory effect on tyrosinase doc
... the formation of dopachrome Changes in the UV/Vis spectra of a mixture of compound and Dopa oxidized by tyrosinase indicated that this was indeed the case (Fig 3C) In the initial phase of the ... So, although both the steric and polar characteristics of the diphenolic substrate modulate the rate of oxidation [23,25], it appears that the size of the side substituent is not the primary factor ... mV for Dopa and 195 mV for compound 1), but indicate that the formation of the phosphonic o-quinone is not favored Thus, two other factors drive the reaction: much slower enzymatic oxidation of...
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báo cáo khoa học: " A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3, encodes a putative chloroplast translation elongation factor that is important for chloroplast development in the cold" docx
... activities, on the other hand, are not sufficient for chloroplast biogenesis and condition the formation of non-pigmented plastids Our working hypothesis is that the green sectors of var2 have ... and var2-5, again suggesting that the interaction between these alleles is complex The acquisition of this second allele of svr3 supports our conclusion that At5g13650 is SVR3 SVR3 encodes a putative ... though the svr7-1 single mutant is resistant to cold treatment, the svr3-1 svr7-1 double mutant is susceptible to it inasmuch that the double mutant shows a chlorosis phenotype similar to that of the...
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Báo cáo y học: " JNK activation is responsible for mucus overproduction in smoke inhalation injury" potx
... was responsible for carrying out the experiments, for data analysis, and for drafted this manuscript; KYK was responsible for the analysis and design for the histologic study; OS oversaw the ... quantitative analysis of the airway mucous secretion, all histological slides of the left lung were randomly sorted and masked before observation The quantity of mucin production in the airway was ... to determine the ΔΔCT The ΔΔCT was then used in the formula ΔΔCT to determine the fold change in mRNA expression The upper and lower limits of fold change were determined by taking the averaged...
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Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... suggesting the existence of an abnormal degradation of the protein [22,24] The second is the putative participation of local factors on the appearance of the disease The TGFBI protein, in addition to the ... degree of amyloid fibril formation Cysteine has the capacity to form disulfide bonds As disulfide bonding of the protein may participate in the appearance of aggregates and predispose to the formation ... higher for C110)131, and H110)131, compared to that of S110)131 and R110)131 Therefore, the results of both The role of disulfide bonding was examined by comparing amyloid formation of C110)131 to that...
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báo cáo hóa học:" Survivin gene levels in the peripheral blood of patients with gastric cancer independently predict survival" doc
... require the lysis of the cells harvested from the peripheral blood of patients in order to extract the mRNA used to assess the expression of target genes Besides CTC, other potential sources of PCR-detected ... aggressiveness of the tumor, and one of these, the Survivin, add independent prognostic power to the TMN staging system This might allow for a better stratification of patient's risk and thus a better therapeutic ... study, the so called "calibrator" is represented by the gene expression of a chosen sample by which the expression profile of each sample of interest is adjusted: this approach enables the investigator...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
... donors The effects of the administration of different doses of IFNa, as well as of repeating the administration of the cytokine in successive treatment cycles, were evaluated The kinetics and the ... according to the percentage of the genes of the lists fitting each class (blue) in proportion to the global composition of the array (light purple) The p value of the modified Fisher test classes ... trend of increase of the expression of HLA-DR (Figure 6D) No significant increase of these molecules was detected by cytofluorimetric analysis in the CD14+ monocytes isolated from the group of subjects...
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MODELING OF THE ELECTROCHEMICAL CONVERSION OF CO2 IN MICROFLUIDIC REACTORS
... the context for the contributions reported in this thesis Chapter is the core chapter of this thesis It details the mathematical formulation of the electrochemical reduction of CO2 in a microfluidic ... practical applicability of the system 1.5 Organization of Thesis In addition to this introductory chapter, which motivates the aim of this research, this thesis is comprised of another seven chapters ... situation, the inlet is closed, and the anode-side gaseous products exit at the other end For the conversion of CO2 to CO studied in this work, the feed gas is a mixture of N2 and CO2 The CO2 in the...
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Tài liệu Research " The Dissertation Committee for Fang Yin Certifies that this is the approved version of the following dissertation: Business Value of Information Technology in the Internet Economy " doc
... digitization due to the restriction of the physical nature of their product Therefore, it is hypothesized that IT capital contributes more to the performance of digital dot coms than to that of physical ... where q is the output, xi is the level of input I, α i is the output elasticity of input I, A is a constant, and where N is the number of inputs The Cobb-Douglas production function is the most ... Ph.D The University of Texas at Austin, 2002 Supervisors: Andrew B Whinston & Anitesh Barua This dissertation consists of three essays that address the issue of the business value of Information...
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Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx
... specificity of the interaction The lack of effect of the diphosphate nucleotide indicates that, following inorganic phosphate release, the ADP-bound ABCG2R482G isoform adopts a conformation capable of ... although the binding data cannot unequivocally inform on the orientation of the sites, only their affinity for interaction with drugs The data presented here suggest that the ABCG2R482G isoform undergoes ... coupled to the catalytic cycle within NBDs The best evidence for an interaction between the two domains is the ability of numerous substrates and modulators of ABCG2 (and the R482G isoform) to...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
... hallmark of the initial response to DSBs is the rapid activation of ATM (product of the ataxia telangiectasia mutated gene), the protein kinase that is mutated in the human hereditary disease ataxia ... In the course of this experiment, irrespective of the presence of the supernatant from the initial centrifugation, the yield of EJ products did not appreciably change (compare lanes and 2) The ... electrophoresis for size-based analysis of DNA Except for the supercoiled form, all aberrant types were preferential targets for aggregation, which occurred depending on the concentration of protein...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... Enlargement of the original plots shown in (B) present in cell extracts of succinate/glucose-grown cells; therefore, the synthesis of the enzyme was inducible Table shows a summary of a typical enzyme ... in a coupled enzyme assay, the enzyme reaction product identified by GC-MS analysis, and the determination of released ammonia The coupled enzyme assay revealed the mechanism of the deamination ... A peak at 527 nm was observed in the emission spectrum These results suggested that the enzyme contains a flavin derivative The flavin cofactor of the purified enzyme was subsequently characterized...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... M/F at the as1-Cas locus Therefore, analysis of their transcripts could have been rather difficult because of the occurrence of at least nine different forms of messenger arising from the F allele ... hydrolysis), corresponding to that expected for the F variant This result is the first evidence for the heterozygous status (M/F) of the individual goat milk analysed In addition to this, the HPLC ... 23 in the 9th exon of the A allele, which is deleted in the F allele, is mutated to T in the M allele, giving rise to a Ser fi Leu substitution Analysis of the intronic flanking sequences The flanking...
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To Cut or Not to Cut? That is the (Central Bank’s) Question In Search of the Neutral Interest Rate in Latin America pdf
... Moreover, there is a strong endogeneity between the level of the policy rate the SELIC—and the level of public debt, given that about half of the domestic public debt is indexed to the SELIC This restricts ... system of equations: where is the nominal short-run (90-day paper) interest rate, the neutral nominal interest rate, stands for the rate of inflation, is the inflation target of the central bank, is ... Analytics For the period prior to the publication of an official inflation target the average yearly inflation number was us ed as the target for the year For 2012M6-13M12 the las t publis hed value...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25