activation of the cyclic adenosine monophosphate second messenger system by parathyroid hormone
Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
... could be blocked by MEK inhibition Second, activation by forskolin ⁄ IBMX at the protein level was not observed beyond h However, the activation at the mRNA level was detectable by northern blot analysis ... at the same concentrations, H-89 failed to repress the Cdx-2 promoter Thus, H-89 inhibits the proglucagon gene promoter presumably by blocking the PKA signaling pathway That the expression of the ... Journal 272 (2005) 2746–2759 ª 2005 FEBS L Chen et al Activation of Cdx-2 expression by cAMP Fig Comparison of the effect of H-89 on expression of the proglucagon and Cdx-2 gene promoters Three micrograms...
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Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"
... staffed by parent unit doctors In addition, there was a trend toward increased frequency of activation of the MET service during these hours in the years following the introduction of the MET system ... was then constructed from the 2568 episodes of MET service review to illustrate the frequency of activation at various times over the 24-hour period Episodes of activation were related to the ... criteria to increase the ease of utilisation of the MET system at our institution Furthermore, the timing and frequency of patient observations reported in the study would be typical of most hospitals...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... play an important role in the formation of the MT1-MMP–furin complex; therefore, 3168 disruption of the interaction between GRASP55 and furin should perturb the activation of pro-MT1-MMP and reduce ... important for the interaction of MT1-MMP ICD with the PDZ2 domain of GRASP55 The interaction of PDZ domains with ICD internal motifs has previously been reported For example, the interaction of the ErbB2 ... the previously observed intracellular activation of pro-MMP2 [59] Mutation of the LLY573 motif to AAA573 in the MT1-MMP ICD has been reported to significantly decrease the internalization of the...
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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... with such a mechanism The most parsimonious explanation of the loss of the potentiating effect in the mutant receptor is that the mutation results in the loss of the ability of dTC to act as such ... Representation of the extracellular domains of the c–a subunits based on the crystal structure of AChBP The positions of cE93 and aR55 at the subunit interface are indicated and modeling suggests that these ... channel activation or receptor desensitization In the case of the Torpedo nAChR, the importance of the a-subunit in ligand binding has long been recognized [11-15], but the involvement of non...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... At the bottom is the quantification of the level of the activation of JNK activity induced by CD53 ligation in three independent experiments The relative values are calculated by the ratio of the ... determine if the activation of JNK by ligation of the human CD53 antigen was a common signal response shared with other cells of the lymphoid lineage, we used the Jurkat Luciferase assays of transcriptional ... functional in the system, but the way they induce JNK activation is different from that of tetraspanin antigens The activation of JNK was normalized with respect to the efficiency of transfection,...
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Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... phosphorylation of the C-terminal SSXS-motif of the R-Smad Based on the diverse mechanism of Smad activation by either TGF-b1 or NGF, specific subsets of target genes might be induced The potential of NGF ... complexes with Smad4 following the activation by TbRI Fig Role of Smad4 in NGF-triggered activation of the Smad pathway (A) The role of functional Smad4 was investigated by reporter gene assays using ... and the cells were analysed by confocal microscopy The projection of multiple sections is seen on the left for each panel to visualize the morphology of the cells The middle row shows staining of...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... when used at 10 mm The blocking of the ATPase activating effect of glucose was not the result of a toxic effect of 2-deoxy-d-glucose, as subsequent incubation in the presence of 10 mm glucose resulted ... that 88 ± 12% of the complex was dissociated by glucose treatment These results represent the mean ± SD values from three independent experiments Further evidence for the occurrence of the H+-ATPase–acetylated ... an early stage of the mechanism that leads to, respectively, inhibition and activation of the enzyme The increase of enzyme activity (determined by ATP-hydrolyzing capacity or by H+-pumping activity)...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
... define the role played by each transcription factor and coactivator in activation of the IFN-b promoter We show that activation of the IFN-b promoter was critically dependent on the nature of the ... role in the context of a given promoter if the arrangement of cis-acting elements allows them to occur Synergistic activation of the IFN-b gene promoter in insect cells The ability of the IFN-b ... ng p65) in the presence or absence of 0.75 or 1.5 lg of the p300, CBP or p300/CBP coactivators The value in the absence of TFs was 0.09 (B) Activation of the )110IFNbCAT reporter by cotransfection...
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Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot
... sequences of the upstream region and of the rst 1938 nucleotides of the rst intron of the rat GS gene were reported [5,6] This intron sequence ends at the EcoRI restriction site (Fig 1) The remaining ... +132 in the GS cDNA, that is, the translation start site in the second exon) between the HindIII and NcoI sites in the polylinker upstream of the luciferase cDNA The 305 bp bovine growth -hormone ... In the case of the C2/7 cells, the concentration of foetal bovine serum was reduced to 1% to induce the formation of myotubes Harvest of the cells was carried out 48 h after transfection in the...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... with those of other GPCRs indicates that the C-terminus of the adenosine A2a receptor consists of about 120 amino acids, which compares to only 30±45 amino acids for the other three known adenosine ... corresponds to the N-terminus of the mature maltose-binding protein Binding of the fusion protein to the IMAC gel in buffer containing 50 mM imidazole indicates the presence of the C-terminal ... nonspeci®cally to the XAC-agarose Theophylline was removed by gel ®ltration before quantifying speci®c [3H]ZM241385 binding in the XAC-agarose eluate The value obtained was only 50±80% of the theoretical...
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Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx
... of K31, together with analysis of PKCe knockout MEFs with knockdown of PKCd, revealed further evidence of a crucial role of K63-ubiquitination of the RING domain of TRAF2 for recruitment of the ... dependent on the RING domain of TRAF2 and the dimeric Ubc13–Uev1A conjugating enzyme complex [28,30] By contrast, elucidation of the structure of the RING domain and the first zinc finger of TRAF2 ... understanding of the molecular mechanisms involved in TNFR1induced activation of the IKK complex and the classical NF-jB pathway might thus become possible only with the help of mathematical modeling The...
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Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... activity of the kb of the 5¢-flanking region was dramatically decreased by the deletion of GC-box3, the GC-rich Fig Alignment of the 5¢-nucleotide sequences of the human, rat and mouse NOX4 genes The ... Fig 3A, the transcriptional activity was dramatically decreased by the deletion of GC-box1 The activity was further decreased by the deletion of GC-box2 (Fig 3B) As shown in Fig 3C, the transcriptional ... suppressed the expression of Sp1 and the antiSp3 shRNA-expression plasmid suppressed the expression of Sp3 The promoter activity of the NOX4 gene was significantly reduced by cotransfection with the...
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![Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt](https://media.store123doc.com/images/document/14/rc/kt/small_AqRuqpZDo1.jpg)
Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt
... abolished by inhibitors of RNA and protein synthesis [6], suggesting the de novo expression of genes involved in suppression of the cell death machinery This hypothesis was confirmed by proteomics-based ... serum, the increase of c-Fos content in ouabain-treated HeLa cells was insensitive to the presence of the ERK inhibitor PD98059 (Fig 7C) These negative results are consistent with the lack of activation ... regulation of the 5¢-UTR by transcription factors, gene activation or silencing is under the complex control of three-dimensional positioning of genetic materials and chromatin in the nuclear space These...
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báo cáo hóa học:" Heterogeneous activation of the TGFβ pathway in glioblastomas identified by gene expression-based classification using TGFβ-responsive genes" pptx
... downregulated by TGFβ in another study, the direction counts will cancel each other during summarization therefore the total direction counts will be fewer than the total counts of the studies ... Cooperation of the ErbB2 receptor and transforming growth factor beta in induction of migration and invasion in mammary epithelial cells Proceedings of the National Academy of Sciences of the United ... growth, survival and migration [4] The importance of activation of EGFR-PI3K/PTEN pathway in the pathogenesis of glioblastoma has been confirmed in the subgroup of patients who showed clinical responses...
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Báo cáo sinh học: " Dengue virus serotype infection specifies the activation of the unfolded protein respon" pot
... proliferation through the activation of specific components of the Unfolded Protein Response [31,32] These observations are also supported by the occurrence of replication and maturation of flaviviruses ... determinant for the physiological consequences of infection Since the activation of the UPR is on the one hand essential for cell survival during viral infection and on the other hand detrimental ... inducer of GADD34 expression than DENV2 (Fig 3A and 3B) In order to test whether this was caused by different replication of the two viruses, we compare the growth of the two viruses by plaque...
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Báo cáo hóa học: " Dengue virus serotype infection specifies the activation of the unfolded protein response" pdf
... proliferation through the activation of specific components of the Unfolded Protein Response [31,32] These observations are also supported by the occurrence of replication and maturation of flaviviruses ... determinant for the physiological consequences of infection Since the activation of the UPR is on the one hand essential for cell survival during viral infection and on the other hand detrimental ... inducer of GADD34 expression than DENV2 (Fig 3A and 3B) In order to test whether this was caused by different replication of the two viruses, we compare the growth of the two viruses by plaque...
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Báo cáo hóa học: " Higher polymerase activity of a human influenza virus enhances activation of the hemagglutinin-induced Raf/MEK/ERK signal cascade" pot
... on the cell surface We recently showed that membrane accumulation of the HA protein triggers the activation of MAPK signaling [24] In this study, we therefore analyzed the expression of HA on the ... expression The relative polymerase activity of the wild type H3N2 was higher than that of the wild type H1N1 The values obtained from the transfections comprising the wild type system of each virus ... In addition to the crucial function of PB1, the PB2 subunit is responsible for recognition and binding of the cap structure of host mRNAs [32,33] The role of the PA subunit in the transcription...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
... over-expression on the activity of the GnRHR promoter was determined by comparing the luciferase activity of the promoter in the presence of pFoxl2 (over-expression) to the activity of the promoter in the absence ... effectiveness of this analysis, Foxl2 derivatives consisting of the entire open reading frame of Foxl2 fused to either the strong transcriptional activation domain of Herpes simplex virus VP16 [9] or the ... for this study The GnRHR gene was not found in either of the other group’s data sets and is noted in Table because further evidence of Foxl2 regulation of this gene is provided in the section that...
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Báo cáo y học: "Methotrexate enhances the anti-inflammatory effect of CF101 via up-regulation of the A3 adenosine receptor expression" doc
... compared with the control group and each of the other treatment groups (Figure 1a) In the therapeutic treatment study, in which all of the treatments were initiated at the onset of disease, clinical ... the mode of action of MTX It acts as an antagonist of folic acid, subsequently inhibiting the synthesis of purines and pyrimidines It was further suggested that the anti-inflammatory effect of ... to the culture system for the last hours PBMCs from MTX-treated RA patients were incubated for hour with CF101 (10 nmol/l) At the end of the incubation time in all of the above experiments, the...
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báo cáo khoa học: " Activation of the hedgehog pathway in chronic myelogeneous leukemia patients" ppt
... bromide staining The density of PCR products were measured by Bio-Rad gel imaging system( Bio-Rad, USA) of photographs of ethidium-bromide-stained agarose gels The relative gene expression of Shh, Ptch1, ... determined by comparing the ratio of PCR products of the target cDNA segments and the b-Actin cDNA segment as a reference Statistical analysis The data are presented as means ± SEM The differences ... in different phases of CML Further analysis of the data revealed an association of Hh signaling activation with progression of CML We compared the transcript levels of Hh and its receptors in patients...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25