... to be invariable as all the enzymes analyzed so far have a a3b3 (F1F0) or A3 B3 (A1 A0, V1V0) stoichiometry [28] The uncertainty lay inthe number of ion-translocating subunits inthe membrane-embedded ... reference-free averages generated by translational and rotational alignment of single c rings showed the same stoichiometry (Fig 2D,E) From both the raw data and averages of c rings, it was clear that they ... SDS–PAGE, with an apparent molecular mass of 57–59 kDa, depending on the acrylamide concentration used (Fig 1A) Western blotting analyses of the intact as well as the denatured c rings revealed the...
... domain B) from AMY2 (in green) and TAA (in black) The superimpositioning was guided by the catalytic acids (D179AMY2, E204AMY2, and D289AMY2 and D206TAA, E230TAA, and D297TAA) The invariant Y51AMY2 ... the Met52AMY2 (Met53AMY1) and Trp83TAA (Fig 1A) Also the larger TAA loop in TAA appeared to hinder binding of the substrate beyond subsite )3/)4 as illustrated by global views of AMY2 and TAA ... conserved in plant a- amylases, has a role in substrate binding and activity This local region was different in structures of nonplant a- amylases having a longer ba loop which typically contained aromatic...
... endotracheal intubation, mechanical ventilation, therapeutic hypothermia, and various intravenous pharmacological infusions [10,11] Therefore, with the rapid use of AEDs by random bystanders, the ... one of the greatest advances incritical care medicine during the past decade Similarly, recent technology has also enhanced the quality of basic CPR For the past four decades, basic CPR has been ... was the fact that many of the persons who had operated the AEDs for these survivors had never been trained how to use them [9] In essence, technology has now made the average person readily capable...
... March 2010 Page of References Du B, Xi X, Chen D, Peng J; on behalf of China Critical Care Clinical Trial Group (CCCCTG): Clinical review: Critical care medicine in china mainland Crit Care 2010, ... Gomersall Critical Care 2010, 14:131 http://ccforum.com/content/14/2/131 that we cannot help As acute care specialists we can contribute to education in acute care as well as intensive care Initiatives ... resulting from acute illness or injury, acute care surely should have a place inthe curriculum Competing interests The author declares that he has no competing interests Published: 11 March 2010...
... matrix of pair-wise comparisons A = (aij) can be established as follows: A1 A2 An A1 a1 /a1 a1 /a2 a1 /an A2 a2 /a1 a2 /a2 a2 /an An an /a1 an /a2 an/an 149 where ai/aj = aj/ai for all i, ... parking area development (to minimise the adverse affects of on-grade parking areas) Quality of parking area development Parking areas are in multi-storey carpark surrounded with some landscaping, ... cross-ventilated areas, the index of the distance from the window line and ratio of openable window area or other controllable openings to net primary area: 0.13 The number in benchmark is calculated...
... percentage of greenery area inthe site In daylight factor, other constituting components inthe measurement include the total area and reflectance of interior surfaces, the total area and average ... being the third lowest among the scores of all case studies This is due to the large surface parking area, resulting in small percentage of greenery area inthe total site area, and the rather ... encourages minimising the use of land and to maximise greenery area, part of the later criterion takes astep further in encouraging the providence of greenery area that contains high ecological value...
... is intended to indicate the future potential sustainable performance of the project, based on the information available at the end of the Design phase Because the information available during the ... sustainable public housing in Singapore In substantiating the main discourse of environmental performance and sustainable architecture, the thesis has revealed numerous negative as well as positive ... Systematic approaches to sustainable housing performance and preliminary development of ArchSAM The systematic approach to each of the five sustainable housing performance issues (Part III) is the...
... ‘environment’ or development , but rather the challenge to find ways of integrating these to achieve ‘sustainable developmentThe three major themes have become the hallmarks of the sustainable development ... performances of a building as an illustration Ina scenario that the building is located in tranquil site, the building can adopt a permeable design with ample and large openings, allowing the user ... effects have also been acknowledged as an inevitable factor (Dorf, 2001) Similarly, the techno-centric approach has many contributions as an initiating and radical approach, but also has drawbacks...
... M, Hirai M, Tominaga N, Nakajima H, Elisseeva OA, Masuda T, Nakano A, Kawakami M, Oji Y, Ikegame K, Hosen N, Udaka K, Yasukawa M, Ogawa H, Kawase I, Sugiyama H: Wilms tumor gene peptide-based ... 2000;95:2198-2203 Oka Y, Tsuboi A, Kawakami M, Elisseeva OA, Nakajima H, Udaka K, Kawase I, Oji Y, Sugiyama H: Development of WT1 peptide cancer vaccine against hematopoietic malignancies and solid cancers ... peptide vaccine and the resultant cancer regression Proc Natl Acad Sci U S A 2004;101:13885-13890 Kawakami M, Oka Y, Tsuboi A, Harada Y, Elisseeva OA, Furukawa Y, Tsukaguchi M, Shirakata T, Nishida...
... understand and individuals and groups using in sports language through collocations of words and it will We have found that learning to analyze and interpret the collocation of words inthe language ... examined the lexical and grammatical translation approach has played a key role inthe teaching of English collocation competence of students at DSPEU One hundred and four in Vietnam for decades ... point out of the collocational errors inthe language of sports between English and Vietnamese as in any other languages Choosing the right collocation will make the speech and writing more natural...
... created It also has the advantage of being owned and operated by a company that knows a thing or two about always-on availability! Those reasons alone make it a great place for a new IT infrastructure ... done it and saved a snapshot of their running instance as an Amazon Machine Instance (AMI) That means if someone has already saved an OpenVPN AMI, for example, then you don’t have to anything more ... machine is named Gateway and I used to log in with the name Gateway \Administrator Now, however, I’ve created a domain named dkrdomain.local, so I now have to use the name dkrdomain\Administrator...
... PAGE analysis of the mitochondrial membranes isolated from all these deletion strains and from a wild-type strain Inthe DQCR9, DISP and DBCS1 strains, a protein band of approximately 500 kDa ... temperature; each gel strip was then placed horizontally inthe gel-pouring apparatus for the second dimension (SDS ⁄ PAGE) [48], already containing the separating gel (15% polyacrylamide and 0.1% ... assistance of specific chaperone proteins is also required The available data indicate that the accessory factor Bcs1p is involved inthe binding of ISP to an immature bc1 intermediate Yeast cytochrome...
... to any other sequences available in FASTA and BLAST database programs at the DNA Data Bank of Japan Recently, we reported the cloning and sequencing of the gene encoding 4-amino-3-hydroxybenzoate ... decarboxylase) and a deamination step (2-aminomuconic 6-semialdehyde deaminase) in P fluorescens strain KU-7 [7] The decarboxylation mechanism inthe metabolic pathways for 3-hydroxyanthralinic acid ... activity In contrast, the deaminase from strain 10d contained an FAD-like cofactor, similar to D-amino acid oxidases [25–27], as indicated by the absorption peak of the purified enzyme at 266 nm The...
... parabutoporin, this could explain the higher antibacterial activity on Gram-negative bacteria for parabutoporin compared to opistoporin Also with magainin analogs, an increase in antibacterial activity ... Gram-negative bacteria, while melittin and mastoparan are more active against Gram-positive bacteria As it has been described that the NH2-terminal a- helical domain of dermaseptin S (amino acids 1–18) ... for antimicrobial activity and is even more antibacterial than dermaseptin (34 amino acids) [19], peptides consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino...
... syntax and semantics arising from verbs, by providing examples of the verb's usage, Since IRACQ assumes that a large vocabulary is available for use inthe training examples," a way to rapidly infer ... predicating something of a bare plural, KIND is used; for instance, cats as in cats are ferocious is represented as (KIND CATS) An indefinite set arising as a bare plural ina VP is represented using ... Stallard, David Answering Questions Posed in an Intensional Logic: A Multilevel Semantics Approach In Research and Developmentin Natural Language Understanding as Part of the Strategic Computing...
... strain BAFR1 (mt+ nit1 nit2 sta2–29::ARG7) contains a disruption of the STA2 gene that was generated through random integration of the pARG7 plasmid inthe nuclear DNA of C reinhardtii [18] Strain ... of starch granules stained by Patag (data not shown) The results listed in Table show that for the first 12 h the cells are actively engaged in amylopectin synthesis and that the overall rate of ... of an extra 11.4 kDa at the C-terminus of the mature algal enzyme When the specific activities (activity vs amount of starch) were measured in comparison with potato, cassava, taro and wheat, the...
... of the quadratic maps which the ordinary unimodal maps not enjoy: • Quadratic maps are analytic and they have nondegenerate critical point; • Quadratic maps have negative Schwarzian derivative; ... box mapping Denote the central domain of the map g as A1 and consider the first return map onto A1 This map is again a real holomorphic box mapping and we can again consider the first return map ... construction of the domains A0 and B0 that at the point r the boundaries of Ax0 and B x0 are tangent to each other and that this tangency is quadratic We will look for the map h0 near the point r in the...
... is the parameter appearing inthe formulation of the vanishing conjecture 0.2 Let us briefly indicate the main steps of the proof First, we show that instead of proving that the functor Avd vanishes, ... exhibited a local system E , for which the functor Avd was zero.1 E In particular, we obtain that inthe -adic situation over a finite field the vanishing of the Euler-Poincar´ characteristics takes place ... be the corresponding projections from IntModd to n Bunn , and s the map IntModd → X d All these maps are Σd -invariant n We have a natural map rInt : ItModd → IntModd n n Lemma 1.9 The map rInt...
... F-stat Control Marketing Treatment P-value A VARIABLES AVAILABLE AT TIME OF RANDOMIZATION Client savings balance (hundreds) Active account Barangay’s distance to branch Bank’s penetration in barangay ... barangay Standard deviation of balances in barangay (hundreds) Mean savings balance in barangay (hundreds) Population of barangay (thousands) B VARIABLES FROM SURVEY INSTRUMENT Education Female Age ... impact of the savings product on financial savings held at the financial institution (both inthe SEED account and in other accounts) We measure change in total balances held inthe financial institution...
... said sharply, marvelling a little at his unconcern inthe face of such grave danger Again he disappeared, and Katherine heard a rain of heavy blows beginning to fall upon the door; then with a ... constantly guarded against in drawing a sledge when travelling on snowshoes In going down the slope the sledge had travelled proportionally faster than the man, and, catching against the framework ... made fast; then Katherine and Miles set themselves to the task of keeping their eyes open to find out who had stolen the lard Later in that same day a miserable-looking Indian came in with a...