a functional analysis of the cameron site chipped stone assemblage

... acag|gtaag tatg|gtaag tatg|gtaag agag|gtaag g(t)5aacag|aagaa c(t)4gtatacag|actc a( t)4cag|atcc c(t)4ag|aatc Not in coding region I I II (2929) (986) (1985) (1490) Table Comparison between exon ... (5Â-CACGCAAGCTTCT CTTGAGTCTCTATGAAGG-3Â) and the 5Â specic oligonucleotides (5Â-CCGGAGCTCGAGACTCTTAGTAA ATGTGCCCC-3Â) for amplication of the fragment from )949 to +33 (5Â-CCGGAGCTCGAGCCGCTAAAGA ... stranded oligonucleotides spanning the region from )70 to )36 of the xMGP promoter (5Â-GATCCAGGGGAGGGAAAACAAGGA GATGAGGAGGTGTGGT-3Â, and 5Â-GATCTACCA CACCTCCTCATCTCCTTGTTTTCCCTCCCCTG-3Â) as BamHI/BglII...

Ngày tải lên: 08/03/2014, 10:20

... the substantial progress that has been made towards elucidating the mechanism of glucosidic bond cleavage and the roles of the catalytic pair, our knowledge of the molecular determinants of aglycone ... 5¢-AGTTCCGTAGGACGCGGAAGTA AATGTGTC-3¢; mutation F200K, 5¢-CACCGACCTGGG GCTTTGACCCCAGTTCCGTAG-3¢; mutations F461L and F461L in P2, 5¢-CGTTCGGTGAAGCCGGCCAGC CATTCAAAGTTGTC-3¢; mutations W373K and ... the mutants, and simulations of the substrate–enzyme interactions of the wild-type and one of the mutants The results provide indications of the native enzymes’ catalytic action and determinants...

Ngày tải lên: 16/03/2014, 04:20

... binding and cleavage creates an amplification loop that maintains a constant level of sense and antisense piRNAs The flamenco-derived piRNAs, on the other hand, is generated by an unknown primary ... stage 14 of oogenesis when a mature egg chamber forms and is ready for fertilisation b a Figure 1.1.1 Schematic diagrams of the D melanogaster ovary (a) An ovariole, showing a germarium at the ... exoribonucleases Eukaryotic mRNA degradation involves two predominant pathways (Figure 1.4.1), namely the 5’ and 3’ mRNA decay pathways Both pathways require deadenylation, that is the removal of the...

Ngày tải lên: 14/09/2015, 08:25

... aggaaattggacaggaaaacg agatcatccaagggcaaatct tcctttaagcatcttacagctaaagg tttagctgtaagatgcttaaaggagct gaccaaataaaaataatacgacttc aactaattgctggcttgttatg tcattgacgataccagcgcatc tccgggtgcgtttaggtgag ttctatcaacaggctgtccacaggtt ... gtcgggcagtatgtattccaa cactacgactacatgcgcgata gaacttggcgcagatctcc atgtcgaagatcggaattaac ttagtccttgctctgcatatactt aaatgtggctcctttctgctt cttgcactgggttcagagttc aggacattcgggagaccataa atttcaggcagttcgttgct aggaaattggacaggaaaacg ... actttgctggtggaggtacggagacagagtaaattctgt t cataatactccacgcgcaaa cgtcttttggcttcttctcc attgccctcaaatcaagcag gtggacggaggagaagacaa tcattgacgataccagcgcatc tccgggtgcgtttaggtgag tgcccatctacgagggttat agtacttgcgctctggcgg...

Ngày tải lên: 14/09/2015, 08:25

... thesis In krimp mutant ovary, progression of oogenesis was compromised and degeneration of the egg chambers was observed from stage onwards (data not shown) A closer examination of krimp mutant ... polarity in krimp mutant oocytes The level of GRK expression was markedly reduced in 100% (n = 30) of the mutant ovarioles and its localisation to the anterior-dorsal corner of the oocyte was affected ... krimpf06583 is a suitable mutant allele for the characterisation of the krimp phenotypes, this allele was placed over an available deletion that uncovers krimp genomic region, Df(2R)Exel6063 Transheterozygotes...

Ngày tải lên: 14/09/2015, 08:25

... vas, aub, cuff, and mael mutants In all of the examined mutants except mael, KRIMP perinuclear localisation was affected, while the localisation of AGO3 and MAEL appeared to depend on KRIMP, as ... It was also noticeable that VAS localisation depends partially on proper AUB localisation Although VAS foci were apparent in aub mutant, cytoplasmic VAS was visibly more abundant than in the ... mutant alleles or allelic combinations are used for all the mutants Localisation of the nuage components at the perinuclear regions of the germline cells reflects a hierarchical assembly All the...

Ngày tải lên: 14/09/2015, 08:25

... rescue the perinuclear localisation of AGO3 and MAEL, the expression level and anterior-dorsal localisation of GRK, and karyosome compaction of the oocyte; but not precocious osk mRNA translation ... krimp and mael mutants (Figure 3.2. 1a) , similar to what has been reported previously in vas, aub, and armi mutants (Savitsky et al., 2006; Vagin et al., 2004; Vagin et al., 2006) TART was de-repressed ... retroelements in the nuage component mutants armi, aub, krimp, and mael cDNAs that are previously employed for the semi-quantitative RT-PCR in (a) are used for quantitative PCR, in the presence of iQ™...

Ngày tải lên: 14/09/2015, 08:25

... that consist of nuage cytoplasmic foci docking partially around the mRNA degradation components Overlapping nuage/P-body foci are expressed as percentages of the total number of overlapping and ... foci A complete overlap and partial overlap are indicated by a white arrow and arrowhead respectively Bar is μm (c) Immunostaining of non-overlapping Me31B A Me31B focus (green) that lacks the ... non-overlapping P-body foci The range of overlaps (complete or partial) appears to be independent of the foci sizes and nuage/P-body pairs (b) Immunostaining of overlapping cytoplasmic AGO3 (red) and...

Ngày tải lên: 14/09/2015, 08:25

... unaffected in at least one of the piRNA pathway mutant aub Figure 3.3.7 Deadenylation is unaffected in aub mutant LM-PAT assay of cycB In the piRNA pathway mutant aub, deadenylation appears unaffected ... since the poly (A) tail length is comparable to that of the control In contrast, deadenylation is impaired in the deadenylase mutant twin, as indicated by the accumulation of longer poly (A) tails ... monitor deadenylation activity, the length of the cycB poly (A) tail was examined in the aub mutant using LM-PAT assay (Figure 3.3.7; Salles et al., 1999) In comparison to the deadenylase mutant twin,...

Ngày tải lên: 14/09/2015, 08:25

... unaffected in the mRNA degradation mutants PAGE-northern analyses of piRNA production in the mRNA degradation mutants The level of piRNA production is unaffected in the mRNA degradation mutants ... both the piRNA pathway and mRNA degradation proteins 102 Figure 3.3.14 Nuage localisation is unaffected in the mRNA degradation mutants Immunostaining of the nuage components in mRNA degradation ... and pcm Furthermore, the piRNA pathway proteins AUB, AGO3, and KRIMP exhibited normal perinuclear and cytoplasmic localisation in the mRNA degradation mutants (Figure 3.3.14) The normal piRNA...

Ngày tải lên: 14/09/2015, 08:25

... overlapped with the tER and endosomal markers (Figure 3.4.1) This is consistent with the observation by Lee et al (2009) that AGO1 and AGO2 are associated with membranes in the cytoplasm in RNAi-defective ... LAMP2, and ARF6 (green) In the wild-type ovary, KRIMP, PCM, and TER94/CD63/LAMP2/ARF6 overlap In the piRNA pathway mutants spn-E and aub, pi-body assembly is compromised but KRIMP remains tethered ... RNAi-defective mutant HPS4 Figure 3.4.1 Cytoplasmic nuage is tethered to ER/endosomal compartments Immunostaining of the nuage component KRIMP (magenta), P-body protein PCM (red), and tER/endosomal markers...

Ngày tải lên: 14/09/2015, 08:25

... Cytoplasmic compartmentalisation of the fetal piRNA pathway in mice PLoS Genet in press Aravin, A. A., M.S Klenov, V.V Vagin, F Bantignies, G Cavalli, and V .A Gvozdev 2004 Dissection of a natural ... Shcherbata, K .A Fischer, K Nakahara, R.W Carthew, and H Ruohola-Baker 2005 Stem cell division is regulated by the microRNA pathway Nature 435:974-978 Hay, B., L Ackerman, S Barbel, L.Y Jan, and ... M .A Valencia-Sanchez, G.J Hannon, and R Parker 2005b MicroRNAdependent localisation of targeted mRNAs to mammalian P-bodies Nat Cell Biol 7:719-723 Livak, K.J 1984 Organisation and mapping of a...

Ngày tải lên: 14/09/2015, 08:25

... in language B He considers CA as a form of interlanguage study and as a central and substantial component of applied linguistics As a matter of fact, CA has had much to offer to practical teaching ... modal in a certain situation makes clear which meaning is intended An effort has also been made to have a contrastive analysis of the meanings expressed via the modal verbs can, may, must and the ... with other verbs to create verb phrases in the sentence 1.4 Contrastive analysis (CA) As one of the main aims of this paper is to carry out a contrastive analysis on the meanings expressed via the...

Ngày tải lên: 29/01/2014, 00:23

... 5¢-AAGCT TCACCATGTACCCTGCCCACATGTACCAAGTG TAC-3¢, 5¢-AAGCTTCACCATGCCGCACCGGCTC ATCGAGAAAAAGAG-3¢, 5¢-AAGCTTCACCATG GCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or 5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGG TTTACAAGCTG-3¢) ... sets of primers (5¢-AAGC TTGAGCGGATCCCCAGCGCGCAACCACC-3¢ and 5¢-GCAGCAGGATCCTCTAGAGAGTTTAGTC TTTG-3¢ for FLAG-DEC1; and 5¢-GAATTCGGCGG ACCAGAGAATGGACATTTCCTCAACCATC-3¢ and 5¢-TCTAGACTACAGCGGCCATGGCAAGTCACTAA ... CGTGACCGG-3¢ for DEC1-H5 7A; 5¢-GATGAGCCG GTGCGGCAATTTGTAGGTCTCC-3¢ and 5¢-GAGAA AAAGAGAGCTGACCGGATTAACGAGTGC-3¢ for DEC1-R6 5A, FLAG-DEC1-R6 5A and DEC1:4–232R6 5A; and 5¢-GATGAGCCGGTGCGGCAATTTGTA...

Ngày tải lên: 19/02/2014, 16:20

... Corporation; Hialeah, FL, USA) at the ÔServeis Cientifico-TecnicsÕ of the University of Barcelona, using the 488 nm line of an argon laser and standard optical emission filters Percentages of cells at ... described above for flow cytometry After establishing a scan area, the slides were analyzed using a 40 · objective and mW of Argon laser power The entire cell preparation was examined A cell gallery was ... contrast and fluorescence photographs of selected field of cells obtained under the same magnification and contrast acquisition characteristics, and using the autofluorescence of the anthracycline as unique...

Ngày tải lên: 20/02/2014, 23:20

... long-term analysis we assume the structure of the agricultural economy in the United States remains stable That is, the role of energy in agriculture remains the same over time The relation between ... hence, putting them at significant market disadvantage The bill sets a maximum amount of allowances that can be rebated to EITE industries at, 2% for 2012 and 2013, 15% in 2014, and then declining ... energy-related expenses, other farm expenses, and the value of agricultural production stays the same.10 To assess the impacts of HR 2454 beyond 2018, we assume the underlying structure of the agricultural...

Ngày tải lên: 21/02/2014, 01:20

... GAATCATTATGAAGCGGTGAGAGCTAATTTTGAAGG TGCTCGTACGCTGCAGGTCGAC TATTTACAAATTTACCTATACGCTCTGAGTTGATATTAC ATCGATGAATTCGAGCTCG GCTAATAACAACTAATATCACTAACAGAAGACCATTAC CCCGTACGCTGCAGGTCGAC GGAGGATTTATCAAAAGCTACTTCGAAAGCTAGGAACG ... CCTTCTTTGATTGTGCTGCAGAACGTTGAGGCTCTATT TGG CCAAATAGAGCCTCAACGTTCTGCAGCACAATCAAAGA AGG CGGATCCATGGTTAAAGATATTATCGAAAGGCATTTGC CCGCATGCGGCCGCTCAAGATGGTGTAAACGCACTAA GCG CACTAGCAGAACGTACTACGGTGTGGTTTATACAAGAG GGTTCCAGCTGAAGCTTCGTACGCT ... TCTAAGCTTGAGCTCTTTCACATAAGGGAGAGTCG CTGTATTCTATTTTTTCAAGAGTTCGATTCTATTG CAATAGAATCGAACTCTTGAAAAAATAGAATACAG CTTCTCTACGCCCGTGCTC GGAGGATCCGTCGACCATGACGACGACCAAGAG CCTTCTTTGATTGTGCTGCAGAACGTTGAGGCTCTATT...

Ngày tải lên: 07/03/2014, 16:20

... PH17P(+) PA55G()) PA55G(+) PT69G()) PT69G(+) TTGTACGTTGCGAACAACCCCGGACAAT ATTGTCCGGGGTTGTTCGCAACGTACAA TTGTACGTTGCAATCAACCCCGGACAAT ATTGTCCGGGGTTGATTGCAACGTACAA ACCACAGGTGTTGTACATTGCGATCAACC GGTTGATCGCAATGTACAACACCTGTGGT ... GGTTGATCGCAATGTACAACACCTGTGGT TCCTACCGCAGGTCCTGAGCAGCAGGGA TCCCTGCTGCTCAGGACCTGCGGTAGGA TTTGCCCGCACTGGCGGCTTTGCGGTGTC GACACCGCAAAGCCGCCAGTGCGGGCAAA TTGGCATACGTACCACAGGTGTTGTAC GTACAACACCTCCGGTACGTATGCCAA ... role as the catalytic base (c) D75E: aspartic and glutamic acids are dicarboxylic acids, but glutamic acid has an additional carbon in the lateral chain The clear effect of this change in the RNase...

Ngày tải lên: 16/03/2014, 03:20

... large package In this case, note that two small packages are equivalent to a large package.14 With this assumption, the analysis with small packages is similar to the case when the firm only offers ... requires that the per-unit margins from the small package are at least twice as large as the per-unit margins from the large package In this paper, we have assumed for simplicity that the marginal costs ... version that can be found at http://mktsci.journal informs.org/ Acknowledgments The author thanks Wilfred Amaldoss; Ram Janakiraman; Ambar Rao; seminar participants at the University of Arizona, University...

Ngày tải lên: 16/03/2014, 11:20

... [31] MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after tryptic digestion of the labeled and nonlabeled Ag-NPA-1 was performed as described in [20,32] The data were analyzed ... of the medium and K2 the orientation factor, determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the ... nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and comparison with that in A suum using a polyclonal antiserum raised against native...

Ngày tải lên: 16/03/2014, 18:20