65 hyperoxia on fetal type ii cell necrosis
Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
... IL-6 in bronchoalveolar cells and monocytes although the density of TLR9 expression was higher on alveolar macrophages than monocytes This finding may suggest expression of non-functional cell surface ... Walkersville, MD) Stimulation of monocytes and bronchoalveolar cells with TLR ligands To assess ligand-induced TLR expression of monocytes and bronchoalveolar cells, 106 cells were cultured in a ... differentiation process from monocytes to macrophages on the expression of TLRs by in vitro monocyte maturation Expression levels of TLR2, TLR4 and TLR9 were monitored by flow cytometry in the transition...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
... HLADR, CD18 (β2 integrin subunit), CD36 (GPIIIb, GPIV), and CD83 (dendritic cell marker) on monocytes in the peripheral blood mononuclear cell fraction and on differentiated macrophages was detected ... expression of CD14 than monocytes, relative to the isotype control, whereas the HLA-DR expression did not differ between the two cell types Expression of CD18 increased with differentiation, while ... 3b) For IL-8 production, the IC50 was 0.3 µM for control cells and 1.2 µM for RA cells IL-6 and MMP9 production was inhibited only at concentrations between and 10 µM RWJ 6 7657 Pretreatment of...
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Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
... unstimulated control and LPS (1 ㎍/ml)-stimulated cells The cells were cultured for 2, 4, 14, 24, 48, and 72 h, and total RNA was prepared from the cells of the two groups Peripheral blood mononuclear cells ... LPS demonstrated no expression of them These differences in cytokine gene expression are attributable to the difference of cell types Second, IL-6, GM-CSF, IL-12 p35, and IL-12 p40 expression levels ... of Superscript II RT were added to the reaction tube containing the RNA cDNA synthesis was conducted at 42℃ for 50 min, and the reaction was terminated by incubating the reaction tube for 15...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
... reduction in TNF-α mRNA expression As our results demonstrate, DHEA administration is able to prevent such a transcriptional repression of the immune response Animals that underwent CLP and additional ... action on cytokine mRNA expression 48 hours after sepsis induction in both tissue types investigated Immune reactivity in the later phases after sepsis onset, in particular TNF-α expression, ... administration in trauma and sepsis is well known In most study designs, a restoration of the repressed immune response could be reported for several cell types by increases in cytokine secretion after...
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báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
... expression of TAM in NSCLC patients correlated with late stage disease (stage II, III and IV), lymph node metastases, pleural invasion, lymphovascular invasion and poor differentiation Although ... lymphovascular invasion, pleural invasion, and tumor differentiation Histological diagnoses, presence of lymphovascular invasion(LVI), and grade of differentiation were confirmed by two senior ... digestion (0.3 mg/ml, Worthington Biochemical Corp, NJ, USA) for h at 37°C The suspension was filtered through a 70 μm stainless steel wire mesh to generate a single -cell suspension The suspension...
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Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
... experimental conditions, which preclude direct comparisons about differential expression levels between groups like LTNP and ES Additionally, the question remains whether hA3G is constitutively ... codons in vif sequences from these patients (Figure 4) Three out of eight vif sequences in LVL contained one or more premature stop codons in the context of a Trp codon (TGG to TAG) No stop codons ... proportion of hA3G expressing cells or an increase in hA3G expression at the single cell level However, the fact that ES subjects had similar CD4+, CD8+ T cells and monocyte PBMC composition at...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... membrane This was performed by incubating L6 cells at a concentration of · 106 cellsÆmL)1 into NaCl/Pi containing 50 lM digitonin on ice for Cells were washed once in buffer A (1 mM EGTA, mM MgCl2, ... expression, the addition of the mitochondrial H+-ATPase inhibitor oligomycin strongly reduced the oxygen consumption As the oligomycin-resistant portion of respiration reflects the level of proton ... stimulated by the addition of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Fig 3A, upper panel) Stimulation of oxygen consumption by CCCP was maximal at concentrations between 0.5...
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Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf
... obtained on cells incubated with untreated particles and on untreated cells Untreated quartz suspensions, at concentrations of 15, 50 and 100 lgÆmL)1, induced COX-2 synthesis in RAW 264.7 cells ... compilation ª 2006 FEBS 63 ` S Scarfı et al Ascorbate-treated quartz enhances COX-2 expression medium at 18 h than at h incubation (Fig 2B) Under the same conditions (particle concentration, incubation ... expression only in cells stimulated with untreated quartz (Fig 6B,C) Mannitol reduced COX-2 expression in cells incubated with untreated quartz only at 50 lgÆmL)1 particle concentration (Fig...
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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
... regulates transcriptional activation of MCP-1 and MCP-5 expression under hypoxic conditions MCP-5 in mouse astrycotes To study the role of HIF-1α in transcriptional regulation of monocyte chemokine ... HIF-1α+/+ cells (Fig 2B) Hypoxia strongly stimulated the release of MCP-5 into culture media in both cell types; however, MCP-5 levels in HIF-1α+/- cells were only about 50% of the wild -type cells ... isoforms may also contribute to the response to hypoxia, HIF-1α is considered the major regulator of O2-tension sensitive genes in cells [12,13] Decrease in cellular O2 tension or the presence...
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Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt
... cells alone CD147 antagonistic peptide had some inhibitory effect on MMP production and cell invasion in the co-culture In the cyclophilin A (CyPA)-mediated cell migration assays, the addition ... Conclusion We conclude in this study that the increased expression of CD147 on monocytes/macrophages in RA may be responsible for elevated MMP secretion, cell invasion and the CyPA-mediated cell ... USA) and PerCP-conjugated anti-CD14 monoclonal antibody (Becton-Dickinson, San Jose, CA, USA) The red cells in whole blood were lysed with a lysis reagent (Becton-Dickinson) Cells were analyzed...
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Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
... activated cell types contribute to the development and progression of RA Monocytes/macrophages, dendritic cells, T and B cells, endothelial cells, and synovial fibroblasts are major components of ... multiple comparisons, the very strict Bonferroni correction was used [31] Alternatively, the less conservative Holm correction was applied for the correction of the data [24] The application of the ... influence the mRNA expression profile, depending on the inflammatory status and/or cell proliferation, potentially resulting in enhanced immigration/proliferation of T cells, B cells, or synovial fibroblasts...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... Morimoto RI: Repression of the heat shock factor transcriptional activation domain is modulated by constitutitve phosphorylation Mol Cell Biol 1997, 17:2107-2115 Welch WJ: How cells respond to stress ... of infection on viral life cycle [12] Obviously, HSPs constitute specific chaperons for the viral proteins necessary to secure proper folding, translocation and formation of multi-component complexes ... exons (except for the hsp70 encoded by a single exon) to avoid the contribution of genome-templated product in the signal analysis Immunofluorescence cell staining The cells were cultured on...
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Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
... sections were washed three times, for 10 each, with PBS and incubated with type I or V anti-collagen mouse polyclonal antibody, diluted at 1:50 in PBS, and type III anti-collagen monoclonal antibody ... Amplification conditions consisted of denaturation at 95C for 15 s followed by 40 cycles of annealing at 56C for 60 s, and extension at 72C for 60 s Primer sets were designed based on the coding region ... 1:50 in a PBS solution, containing 0.006% Evans blue and mounted with a buffered glycerol solution The reaction was visualized in a Nikon fluorescence microscope Collagen I, III and V Real-time...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... transcription of some genes in these lymphomas was associated with the malignant transformation of these cells or with another factors, such as different proliferation rates of the cell populations ... Another open question is whether there are differences in the expression of genes in HIV/SIV-related and spontaneous lymphomas To address these questions, the subtraction hybridization between the ... myeloid cell line KG-1 [18] Northern blotting with RNAs from SIV-associated monkey lymphomas [13] demonstrated overexpression of the pub gene in the cells of all three SIV-associated monkey lymphomas...
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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
... AY177933 U 7655 3 U 7655 2 354bp Nitrosomonas sp Nm33 AF272408 491bp Nitrosomonas sp Nm41 Nitrosomonas sp Nm58 Nitrosomonas aestuarii Nitrosomonas communis AF272410 AY123820 AF272400 AF272399 CONCLUSIONS ... Association (1995) Dissolved organic carbon (DOC) was analyzed as non-purgable organic carbon (NPOC) by TOC-5000 (Shimadzu, Japan) In situ hybridization In situ hybridization was conducted to monitor ... of amoA mRNA, then continuing care should be taken into consideration in order to make a direct correlation between mRNA detection and in situ activity of ammonia oxidation Amount of amoA mRNA...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... plasmid DNA containing TI gene fragment Expression of recombinant MCoTI -II (ReMCoTI -II) The expression conditions such as ampicillin and IPTG concentrations, temperature and time of induction were ... (containing 58kD fusion protein band) Cells were broken by sonication in 10mM Tris-HCl buffer pH8 containing 1mM EDTA (TE buffer) After centrifugation, the clarified supernatant was loaded onto ... Centrifugation Supernatant Cells Sonicated in TE buffer Centrifugation Pellet Supernatant Chitin column chromatography Washing column, on column cleavage (40C/40hours) ReMCoTI -II Freez dryer ReMCoTI-II...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles in myelination, trophic support and axon regeneration, ... and TNF-alpha on expression of immune molecules and antigen presentation by Schwann cells Cell Immunol 165, 65 70 Kingston AE, Bergsteinsdottir K, Jessen KR, Van der Meide PH, Colston MJ & Mirsky ... afferent neuronal function after inflammation Previous studies have already addressed the participation of SCs in immune responses in the PNS [31] These cells may function as antigen-presenting cells...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... The absolute concentration of apoB turned over was lower under these conditions, due to the lower initial accumulation of nondegraded intracellular apoB (data not shown) Investigation of the nature ... modification is observed for methylglyoxaland glycolaldehyde-modified LDL under the conditions used in these studies, consistent with this previous conclusion To investigate the types of receptor responsible ... accumulation of modified proteins within cells, and subsequent perturbation of cellular metabolism [16,19–21] Previously, we have characterized conditions that yield glycated, but nonoxidized,...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... in control cells within 15 of stimulation This activation pattern is mechanistically consistent with the kinetic parameters of translocation to the cell membrane of these GTPases [25] In contrast, ... expression (P < 0.05) In agreement with this, UV-irradiation of the cells (2.4 JÆm)2), although inducing a strong activation of JNK1/2 and only a very weak phosphorylation of p38, had no effects on ... shown) Higher concentrations were not used to avoid potential nonspecific and/or toxic effects In contrast, anisomycin induced CTGF/CCN2 expression over a wide range of concentrations e.g 1–100...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... response by IL-1b alone under our cell culture conditions We therefore used fetal bovine serum as a positive control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation ... conjunction with other growth factors [2] The present study confirms that the incorporation of AA increases the proliferation of SMC [3,4], but only in response to IL-1b stimulation IL-1b alone ... to activate transcription We tested two constructs fused to the HSV-tk minimal promoter: one contained the wild -type region (Wt Egr-1) and the other contained a mutation affecting the Egr-1-binding...
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