6 cord blood stem cells many categories of stem cells with different characteristics or different approaches to charac
báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc
... were counted according to their morphologic characters Comparison of absolute CFU (total CFU together with three different types of CFU) expansion fold after 14 days culture with or without alemtuzumab ... depletion of CD52+ cells with alemtuzumab in ex vivo expansion experiments of CBU may lead to a higher percentage of CD34+ cells through depletion of more mature CD52+ hematopoietic progenitors Hence, ... after 14 days of culture Many clinical cord blood expansion trials now incorporate the expansion of http://www.jhoonline.org/content/1/1/19 one unit of cord blood following the infusion of another...
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Soluble mediator profiles of cord blood mononuclear cells in early onset childhood allergic disorders
... factors 120 5.3.4 Factor Analysis of cytokine profiles 124 5.3.4.1 Factor analysis of LPS stimulated cytokine profiles 124 5.3.4.2 Factor analysis on PHA stimulated cytokine profile 127 5.3.5 Monocytes ... of studies relating CBMC cytokine responses to a family history of atopic disorders 23 TABLE 1-3 Summary of studies related to in vitro CBMC cytokine responses to the subsequent development of ... Respiratory syncytial virus SPT Skin prick test SCORAD Severity scoring of atopic dermatitis TIMP Tissue Inhibitor of Metalloproteinase TLR Toll Like Receptor Th T helper Th1 Type helper T cells...
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Design of artificial microenvironment for cord blood CD34+ cells expansion ex vivo
... requires strategies to maintain an appropriate balance between stimulatory and inhibitory modulators of HSC function Positive modulators of HSC function Among the different stimulatory cytokines identified ... matrix bound modulators of cell function, many investigators have focused on developing culture systems supplemented with stroma, appropriate combinations of stimulatory factors to maintain and ... concentrations of early acting cytokines SCF and FL result in higher yields of cells that were enriched with primitive progenitor and multipotent hematopoietic progenitor cells in a 3D Cellfoam bioreactor...
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Báo cáo y học: "Clinical observations on intensive immunosuppressive therapy combined with umbilical cord blood support for the treatment of severe aplastic anemia" ppt
... were given double units of the umbilical cord blood transfusion HLA matching and blood typing were performed for umbilical cord blood selection, and cord blood units with 1-3 HLA typing mismatches ... combined with an unrelated umbilical cord blood transfusion The mononuclear cell (MNC) count of the transfused cord blood was 2.25-15.1 × 107 cells/ kg with a median MNC count of 4.0 × 10 cells/ kg ... hematopoietic support and reduced transfusion requirement Further expanded study is needed to characterize the kinetics of hematopoietic reconstitution Table Recovery of hematopoiesis in different...
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Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc
... concentrations of c-secretase inhibitors then analyzed by FACS (A) Relative fluorescence was calculated as the ratio of mean linear fluorescence (MLF) of inhibitortreated cells (grey solid line) to the MLF of ... are fused to the transcription activator factor Gal4-VP16 It is expressed with a signal peptide to ensure correct insertion and orientation into the membrane Proteolytic processing of the substrate-activator ... substrate-activator protein by c-secretase releases the activator domain into the cytosol, allowing it to promote expression of the enhanced green fluorescent protein (EGFP) reporter gene (Fig 1A) Therefore,...
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Báo cáo khoa học: Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides doc
... Yang et al OH O OR O OH OR' (R=H, SO3Na; R'=SO3Na) H+ (R=H, SO3Na) OH O HO OH O O O O OH O OH O O O O OH OR H+ OH OR' OH O HO OH O O O O OH O O OH CH2OH OR OR' OH O O OR' O OH OR OR' (R=H) H O ... important to compare directly, and investigate in detail, a pair of polysaccharides or oligosaccharides with a similar galactan sequence but with Gal substituting for the 3,6-anGal residues Unfortunately, ... j-carrageenan with 0.1 M TFA at 60 °C for h (C) Reductive hydrolysate obtained from j-carrageenan with 0.1 M TFA at 60 °C for h (D) GC-MS total ion current chromatogram of the HPLC fraction of j-carrageenan...
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NEW APPROACHES TO CHARACTERIZATION AND RECOGNITION OF FACES pptx
... the most important steps in a face recognition system and differentiates between semi-automatic and fully automatic face recognizer The goal of an automatic face detector is to search for human ... if found, to accurately return their locations In order to make the detection fully automatic the system has to work without input from the user Many attempts to solve the problem of face detection ... into N vertical super states, or image strips, for the overall top -to- bottom HMM Next the data corresponding to each of these vertical super states is now horizontally segmented from left to...
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NEW APPROACHES TO CHARACTERIZATION AND RECOGNITION OF FACES by Peter M. Corcoran doc
... the most important steps in a face recognition system and differentiates between semi-automatic and fully automatic face recognizer The goal of an automatic face detector is to search for human ... if found, to accurately return their locations In order to make the detection fully automatic the system has to work without input from the user Many attempts to solve the problem of face detection ... into N vertical super states, or image strips, for the overall top -to- bottom HMM Next the data corresponding to each of these vertical super states is now horizontally segmented from left to...
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báo cáo khoa học: "Chemical and transcriptional responses of Norway spruce genotypes with different susceptibility to Heterobasidion spp. infection." ppsx
... mechanisms [1, 2] Many of these are associated with the production of secondary metabolites to delay or stop the establishment of fungi or insects within the tree [2, 12-14] Oleoresins produced ... analyses LC-MS analyses were performed on a Finnigan HPLC system, consisting of a Surveyor MS Pump Plus, Surveyor Autosampler Plus and Surveyor PDA Plus detector, coupled to a 2D linear ion trap, Finnigan ... method for isolating RNA from pine trees Plant Molecular Biology Reporter 1993, 11(2):113116 Sambrook J, Russel D: Molecular cloning: A laboratory manual, edn New York: Cold srping harbor laboratory...
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A brief study of phoenix joint stock company and its advertising approaches to business operations
... customers Consider placing an ad or writing a short article for the magazine Contact a reporter to introduce yourself Reporters are often on the look out for new stories and sources from which to ... (major): Almost everyone read the local, major newspapers You can get your business in the newspaper by placing advertises, writing a letter to the editor or working with a reporter to get a story ... possible to communicate the benefits and features of their new product or brand to consumers For example, South Korea’s Kia Motors Corp entered the US automobile market in 2009 and used ads to tell...
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Self assembly and rheology of block copolymer with polymer additives or particle suspensions
... micellization temperature (CMT) of F127 The effect of PAA is strongly dependent on the pH of the medium Next the incorporation of Pluronic L64 or P105 to F127 results in formation of mixed micelles It is ... Thermograms of F127 in presence of PEO of different M.W.; F127 conc = 20%; PEO conc = 6% 58 Figure 3.5 Effect of MW of 6% PEO on CMT of 20% F127 (top); Effect of PEO conc on CMT of 20% F127 ... (BASF laboratories, Wyandoote, USA)) or Synperonics® (ICI laboratories, Wilton, UK) US Food and Drug administration (FDA) guide has presented Pluronic F127 as an inactive ingredient for different...
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The interplay of collaborative technology with learner characteristics conceptual and empirical examinations
... Revised Theoretical Framework of CT Effectiveness 219 Figure 8.1 Flowchart for the Educator’s Selection of CT 228 xii The Interplay of Collaborative Technology with Learner Characteristics: ... factors that may serve to enhance the effectiveness of CT For CT characteristics, the study delineates two salient characteristics that are relevant to the emerging technology Five learner characteristics ... Uptake of New Breeds of Collaborative Technologies In recent years, developments in the IT world have seen a shift from offline software to online software services One of the forefronts of this...
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Generation of vorticity at a free surface of miscible fluids with different liquid properties
... Formation of a vortex ring 124 Fig 3- The plot of multiple vortex rings region 125 Fig 3- Formation of multiple vortex rings 126 Fig 3- 10 Formation of a thin jet 127 Fig 3- 11 The top view of ... would like to express my gratitude to National University of Singapore for providing me with the financial support during my doctoral education i TABLE OF CONTENTS Acknowledgement i Table of Contents ... thanks also go to staff of Impact Mechanics Laboratory for their input and support on the project I would also like to thank every member of my family and my friends for their support and confidence...
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Acoustic behaviors of polymer microspheres with tailored chain or matrix structures
... Thickness of the disk ρb Bulk density of the disk η Viscosity of the streaming medium τ Tortuosity ε Total porosity of the materials Reff Effective flow resistance ψ Shape factor account of the ... the porous framework inside each individual particle offers a space suitable for different types of structural tailoring to improve the acoustic performance The porous microspheres composed of ... plot of SD11, SD11EA10 and SD11EA50 for the characterization of interstitial voids size distributions 119 4.3.5 Log differential intrusion plot of SD31 and SD31EA50 for the characterization of pore...
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Evaluation of different approaches to protein engineering and modulation
... digest of the Sortase A gene PCR amplification of SortaseA from the S.aureus genome Sortase Sortase Sortase Cloning of Sortase A into the pTOPO vector MCS 10B The T7 genome-10-3-B vector 10B ... Transformation of plasmids/ligated vectors into chemically competent cells The competent cells were thawed on ice The DNA (plasmid/ligated vector) was added into the competent cells (the volume of ... emerge 12 Library of phages bearing peptides on the surface Incubation of the of phage with the receptor Affinity based binding of ligand phages to the receptor Immobilized receptor The process...
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Systemic risks of genetically modified crops the need for new approaches to risk assessment
... on ecotoxicological laboratory approaches Standard protocols and organisms are used due to the good reproducibility of experiments, easy breeding of those organisms and low costs of the work The ... effects”, e.g the effects of the herbicides used together with herbicide tolerant crops, as demanded by the legal Page of 11 framework, how to deal with the foreseeable EU-wide use of antibiotic marker ... commercially in the USA [23-25] The U.S authorities did not require an ecologically oriented laboratory or even field test for the deregulation of Bt cotton in 1995 [26] The respective risk research...
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Báo cáo hóa học: " Quantitative expression analysis of HHV-6 cell receptor CD46 on cells of human cord blood, peripheral blood and G-CSF mobilised leukapheresis cells" docx
... [14] Santoro et al [15] suggested the existence of additional cellular factors, possibly co-receptors that are crucial for HHV-6 infection However CD34+ and CD38+ hematopoietic progenitor cells ... surface antigen Figure of CD46 MACS blood of adult donors (PB)CD34+, CD38+, CD33+ hematopoietic from G-CSFcells from cord blood cells [triangles] and molecules on cell CD34+ cells of [circles], leukapheresis ... Membrane cofactor protein (MCP or CD46) is a cellular pilus receptor for pathogenic Neisseria Mol Microbiol 1997, 25:639-647 Russell S: CD46: a complement regulator and pathogen receptor that mediates...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... syad 41 rof )ASU ,negortivnI ;FGNh( rotcaf htworg laruen namuh lm/gn 01 dna )dnalreztiwS ,ehcoR ;FGEh( rotcaf htworg lamredipe namuh lm/gn 01 ,)dnalreztiwS ,ehcoR ;FGFb( rotcaf htworg tsalborbif ... reyemxorB ,E namkculG 67-66 ,28 ,3991 doolB yawhtap noitaitnereffid elcsum htooms ralucsav a gniwollof etaitnereffid taht sllec lamyhcnesem era serutluc worram mret-gnol namuh morf sllec lamortS ... morf sllec mets lamorts lamyhcnesem fo laitnetop cinegoetso detaler-egA AG drawoH ,AB sooR ,C idrociR ,CP rellihcS ,G otiloppI’D 37-76 ,181 ,9991 loisyhP lleC J sllec rotinegorp lamyhcnesem worram...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
... ,skeew dna ta spuorg GBCU dna CSM-BCU eht naht serocs yblO rewol yltnacifingis dah G puorg ,revewoH skeew ta spuorg rehto eht morf ecnereffid tnacifingis yna wohs ton did G puorG spuorg PC dna NC ... noitatnalpsnart htiw puorg eht ;)5 = n :G( FSCGhmr fo noitcejni suoenatucbus htiw puorg eht ;)5 = n :PC( noitcejni -aidem htiw puorg lortnoc eht ;)5 = n :NC( tnemtaert on htiw puorg lortnoc ehT yrujni ... noitarefilorp llec mets worram enob etalumits ot nwohs la te miL yeH-iJ 672 .)50.0 < p( noitatnalpsnart retfa keew ta spuorg rehto lla htiw derapmoc sa devorpmi yltnacifingis erew spuorg CSM-BCU...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
... were suspended with 500 μl of normal saline prior to mixing with 700 mg of β-TCP (group CM) The same volume of normal saline mixed with β-TCP was prepared as the control (group C) Histological examination ... tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages ... cytokine profiles in osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology Cell Biol Int 2008, 32, 320-325 27 Rogers I, Casper RF Umbilical cord blood stem cells Best...
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