5 on human bladder cancer cells correlates with the ability to internalize bcg
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... Interactions of BCG with the bladder wall Adhesion of BCG with the urothelium The interaction between BCG and the bladder wall can be mediated by specific, receptorligand mediated events or by non-specific ... ways to improve response to therapy Figure 1.2: BCG attachment to the bladder mucosa The interaction is mediated by a fibronectin bridge that links integrin 5 1 receptors on the bladder urothelial ... profiles of human bladder cancer cell lines………………………………………………………………………………… 87 4.1.1 Expression of Integrin 5 on human bladder cancer cells correlates with the ability to internalize BCG ………………………………………………………...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... Tc52 [16] In order to ascertain our observation and to rule out the (possibility that the lack of binding of SmGSTO to glutathione was neither due to the presence of the His-tag sequence nor to ... the S mansoni EST database with the complete sequence of Tc52 revealed a clone (EST AI9 758 43) with around 25% sequence identity with the GST-like domain of the T cruzi protein A fragment of 54 9 ... which contact GSH in GSTO 1–1 correspond to S(79), E(78), V( 65) , K (52 ) and C( 25) in SmGSTO, and were predicted to be placed in the same spatial orientation (Fig 7B) DISCUSSION We report here the...
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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... and on rat pheochromocytoma cells Recent studies have found an association between the ability of Abs to recognize native PrPC expressed on the cell surface and their ability to cure prion-infected ... concentrations of Abs to epitopes encompassing amino acids 95 1 05 of the PrP were used Injection of mAbs that recognize the central region of the PrPC (residues 133– 157 ) and bind efficiently to ... as positive control To detect PrPSc conversion, cells were lysed, and then subjected to proteinase K digestion and immunoprecipitation A deglycosylation step was included to reduce the PrPSc and...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... acid sequencing 65 60 55 50 45 40 35 30 25 20 15 10 0 10 15 20 25 Time (min) 30 35 40 45 50 Fig Purification of cockroach GST by phenyl-Sepharose chromatography (A) The elution profile for GST activity ... Sigma 59 .3 57 .9 56 .9 54 .6 45. 4 45. 4 14.8 13.9 13.0 Sigma Epsilon Epsilon Omega Theta Zeta 13.9 36.6 34.7 10.6 26.4 9.7 Cloning of a Delta class GST from B germanica The cloning of cDNA encoding the ... the activation barrier of the reaction [30] In addition, the rate of product release may contribute to the efficiency of the reaction [31] Functionally, BgGSTD1 may play an important role in the...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
... transformation These results further support the idea that the ability of the GST isozymes to induce transformation is dependent on their ability to conjugate 4-HNE to GSH Furthermore, these results ... presented) The colonyforming ratio of clone C4 (HLE B-3) cells to WT-K562 cells used as positive control in these experiments was found to be : Taken together, these results confirm the phenotypic ... and the GFP expression vector, or with fluorescent markers alone At 24 h after the injection, any cells killed by the injection procedure were lifted off the dish leaving only the injected cells...
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Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
... 0– 350 0–200 0– 350 3 3 450 –640 350 –400 200 55 0 350 – 450 12 11 12 12 650 – 750 400– 450 55 0– 750 450 – 750 5 subunits of the purified isoforms N-terminal amino acid analysis was carried out on a particular ... with varying concentrations of CDNB (0.04 5. 12 mM) and a constant GSH concentration (50 mM) The Km and Vmax with GSH as a substrate were performed with varying concentrations of GSH (0.026– 15. 6 ... reported in the literature for GST from nonvertebrate organisms (0.06– 2.7 mM) This allows one of the isoforms to catalyze the conjugation in optimal conditions, whatever the concentration of endogenous...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
... CDS STOP 3¢-UTR 3¢-UTR 3¢-UTR 3¢-UTR – – 36 55 119–138 152 –171 1 75 194 – 326–3 45 397–426 480–499 52 4 54 3 – – 786–8 05 – – 978–997 – – – 119–138 152 –171 1 75 194 2 85 304 – – – 52 4 54 3 55 4 57 3 655 –674 ... pH 6 .5 CDNB–GSH conjugate formation was monitored over time with a PerkinElmer HTS7000 bioassay plate reader at 340 nm and 37 °C The rate of conjugate formation was constant 15 45 after the addition ... found to be specific inhibitors of either GSTM1 or GSTM2 expression in the EGFP assay The results are shown in Table None of these ODNs affected the CDNB conjugation in cells transfected with pEGFP,...
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Báo cáo y học: "4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase" doc
... Quantitation of cytochrome c release in cytosolic fractions To measure cytochrome c release, chondrocytes (1 × 106 cells) were treated for 16 hours with increasing HNE concentrations (0 to 30 μM) with ... than to 20 μM, HNE was cytotoxic and significantly decreased cell viability (up to 50 %) and induced chromatin condensation, compared with untreated cells Pre-treatment of cells with anti-Fas/CD 95 ... apoptotic response of chondrocytes to HNE requires upregulation of pro-apoptotic protein synthesis or whether it relies on pre-existing apoptotic machinery Our data show that the apoptotic response...
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Báo cáo y học: " Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan" ppt
... 0.2 256 rs16 95 5’-ACC CCA GGG CTC TAT GGG AA-3’ & 5 -CAG GTT GTA GTC AGC GAA G-3’ GSTO1 Exon C®A Ala140Asp (A140D) 0.1660 rs49 25 C_11309430_30 GSTO1 Exon -/AGG Glu 155 del (E 155 del) 0.01 25 rs1 150 9437 ... adjusted HR ( 95% CI) Count(%) Follow-up person-years UC Crude incidence (per 1 05) < 50 390 (51 .1) 7 251 11 151 .7 ref 50 -59 270( 35. 3) 450 8 22 488.0 3.38(1.64-6.98)** 60+ 104(13.6) 155 7 51 3.8 3.68(1.48-9.18)** ... higher cancer risk (HR, 34.43, 95% CI, 5. 03-2 35. 74, p < 0.01) when compared to the reference and the etiologic fraction was estimated to be 0.80 0. 75 GSTM1 Non-null Null GSTT1 0. 05 GSTP1-1 05 GSTO1-140...
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Báo cáo y học: " Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients" potx
... stress The present study was undertaken 1) to investigate the cell specific distribution and expression of GSTO1-1 in healthy human lung, 2) to compare the GSTO1-1 expression patterns in the lung ... methanol for 15 minutes The sections were incubated with the primary antibody for GSTO1-1 using a dilution of 1:200 The immunostaining was done using the Histostain-Plus Kit (Zymed Laboratories Inc., ... statistically significant Correlations to lung functions were analyzed with the Pearson correlation test Ethical considerations The study protocol was approved by the ethical committees of Oulu University...
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Báo cáo y học: "Anticitrullinated protein antibody (ACPA) in rheumatoid arthritis: influence of an interaction between HLA-DRB1 shared epitope and a deletion polymorphism in glutathione s-transferase in a cross-sectional study" ppt
... 64 (11) 53 ( 15) 655 (93%) 173 (28%) 53 6 (76%) 420 (69%) HLA-DRB1 SE-positive 53 1 (76%) 434 (71%) One copy 356 (51 %) 303 (50 %) Two copies 1 75 ( 25% ) 131 (21%) GSTM1-null 372 (53 %) 3 15 (52 %) SE-GSTM1 ... 2.24 (0.97 to 5. 16) 0.788 0. 058 51 74 0.73 (0.39 to 1.37) 2.03 (1.08 to 3.82) 0.329 0.027 Positive/Ever 87 5. 05 (2.31 to 11. 05) < 0.001 75 2.10 (1.17 to 3.78) 0.013 AP = 0 .58 (0.31 to 0. 85) AP = ... Null/Never 75 72 1.42 (0.80 to 2 .52 ) 1. 35 (0. 65 to 2.79) 0.227 0.421 67 67 0.72 (0.42 to 1.22) 0. 75 (0.42 to 1.36) 0.223 0.349 Null/Ever 81 2.01 (1.13 to 3 .55 ) 0.017 70 0.83 (0.49 to 1.42) 0 .50 2 AP...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis was performed after transfection Therefore, all the ... performed to analyze the EGFP-containing inclusions Figure 1F shows that the expression of Hsp70 together with Hdj-1 (Hsp40) did decrease the average size of the EGFP-containing inclusion bodies ... compared to the level observed in COS-7 cells transfected with control EGFP and httEx1-25QEGFP vectors When the Hsp-encoding vectors were transfected together with that encoding httEx1-72QEGFP, the...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... a = b = 53 . 35, a = b = 53 .5, parameters (A) c = 116.22 c = 116.2 Wavelength (A) 0.7100 1.0000 Resolution range (A) 50 1. 05 (1.091. 05) 50 1.71 (1.771.71) Measured reections 950 , 955 2 05, 263 Unique ... of these sites These densities were assigned as zinc ions derived from the crystallization solution as an additive The zinc ion closest to FMN, Zn201, is coordinated by the Sc atom of one of the ... Cys39 and the other conformer of Cys40 This zinc ion is bound to His131, which corresponds to the histidine residue that is completely conserved among the FeR homologs [5, 6] Interestingly, the residues...
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Analysis of Healthcare Interventions that Change Patient Trajectories doc
... Interventions That Change Patient Trajectories 3.20 3.21 3.22 3.23 3.24 4.1 5. 1 5. 2 5. 3 5. 4 5. 5 5. 6 5. 7 5. 8 5. 9 5. 10 5. 11 5. 12 5. 13 5. 14 5. 15 5.16 5. 17 5. 18 5. 19 5. 20 6.1 6.2 6.3 6.4 6 .5 6.6 6.7 ... population projections) The 11 categories are as follows: less than 1, 1–4, 5 14, 15 24, 25 34, 35 44, 45 54 , 55 –64, 65 74, 75 84, and 85+ The other partition is one used by the Census Bureau to report ... 12 Age Categories
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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
... region between Val231 and Ala 358 contains the 6A3 epitope To further narrow down the 6A3 epitope, we then identified two nonconserved clusters within this 128 amino acid region (Fig S2B) Because the ... binding to hDPP IV Although monoclonal DPP IV antibody 4D10 arrests the cell cycle FEBS Journal 276 (2009) 654 8– 655 9 ª 2009 The Authors Journal compilation ª 2009 FEBS 655 5 Epitope of monoclonal ... V334T, blocked the inhibition of cancer cell adhesion to DPP IV and soluble DPP IV binding to polyFN-expressing cancer cells (Table 1) Taken together, these data indicate that the 655 4 Thr331-dependent...
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báo cáo sinh học:" Community characteristics that attract physicians in Japan: a cross-sectional analysis of community demographic and economic factors" potx
... independently correlated with the physician-topopulation ratio, and how much the fluctuation in total of the variables could predict the fluctuation of the physician -to- population ratio among the municipalities ... person, showed strong collinearity with one or both of the two variables, and therefore were not used in the regression model Two other variables, the proportion of those aged 65 or older among the ... amenities correlates well with population size The physician -to- population ratio is known to increase proportionately with population [1 ,5] This means that physicians would rather practise in cities with...
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Báo cáo sinh học: "Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts" ppt
... Other groups have only single sites (Group 8) or lack sites altogether (Group 7) The hypervariability of the UL146 ORF led to an analysis of the sequence components downstream of the UL146 stop ... binding to the inhibitory receptor ILT2 J Virol 20 05, 79(4):2 251 -2260 Bar M, Shannon-Lowe C, Geballe AP: Differentiation of human cytomegalovirus genotypes in immunocompromised patients on the basis ... strains Towne and Toledo were also included in the phylogenetic analysis Among these strains the length of the ORF ranges from 342 to 3 75 nucleotides encoding predicted proteins of 114 to 1 25 amino...
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Báo cáo hóa học: " Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that ?" doc
... dicted to make major contributions to the binding energy of the NS5A-SH3 domain interaction To test this hypothesis we constructed the corresponding site-directed mutants of the Fyn SH3 domain in the ... 2008, 5: 24 http://www.virologyj.com/content /5/ 1/24 Figure Surface plasmon resonance analysis of the NS5A(Δ 250 ):Fyn SH3 domain interaction Surface plasmon resonance analysis of the NS5A(Δ 250 ):Fyn ... shown to make an intramolecular hydrogen bond with R96 within the RT-Src loop of the SH3 domain – it was possible that the effects of the double mutant were due to the inability to form this bond...
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báo cáo hóa học:" High rate of loss to clinical follow up among African HIV-infected patients attending a London clinic: a retrospective analysis of a clinical cohort" ppt
... London, W2 1PG, UK Authors’ contributions SMG completed all the statistical analysis, wrote the first draft of the paper, and made alterations to the paper according to suggestions from the other ... services in the UK for at least a year registration in 1997 to 1999 (OR = 4.81, 95% CI: 3.06 to 7 .57 ) and 2000 to 2002 (OR = 2.90, 95% CI: 1.96 to 4.30) versus registration in 2003 to 20 05; a CDC ... versus > 350 cells/ mm (OR = 1.99, 95% CI: 1. 05 to 3.74); and a detectable viral load (OR = 5. 03, 95% CI: 2. 95 to 8 .57 ) at last attendance Among those not receiving HAART, independent risk factors...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25