3 lineage of stem cells in placental umbilical cord blood
Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
... Transplantation of canine umbilical cord blood- derived mesenchymal stem cells in experimentally induced spinal cord injured dogs J Vet Sci 2007, 8, 275-282 22 Lu J, Descamps M, Dejou J, Koubi G, Hardouin ... progenitors from umbilical cord blood stem cells by BIO-PLEX technology Cell Biol Int 2008, 32, 320-325 27 Rogers I, Casper RF Umbilical cord blood stem cells Best 393 Pract Res Clin Obstet Gynaecol ... Fig Lateral radiographs of antebrachium at 0, 4, 8, and 12 weeks after first injection of canine umbilical cord blood derived stem cells was decreased by weeks after injection The dog could bear...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition,...
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báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf
... Conclusion Our findings showed that umbilical cord blood CD34+ stem cells have the potential to interact with dystrophic muscle cells restoring the dystrophin expression of DMD cells in vitro Although ... amplified in co-cultures, indicating the absence or very low expression of dystrophin in co-cultures (data not shown) Transdifferentiation of CD34+ stem cells into muscle cells During the expansion of ... mesenchymal stem cells isolated from human umbilical cord blood Stem Cells 2004, 22(4):617-624 Nunes VA, Cavaçana N, Canovas M, Strauss BE, Zatz M: Stem cells from umbilical cord blood differentiate into...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... before administration of StemKine supplementation (day 0) and at days 1, 2, 7, and 14 Study participants were required to ingest two capsules of Stem- Kine in the morning and two in the evening for...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... in isolating enough multipotent stem cells from human umbilical cord blood and maintaining cell culture for experimental analysis A guarantee of enough numbers of multipotent stem cells out of ... doubling level (CPDL) and culture of canine umbilical cord blood (cUCB)-mesenchymal stem cells (MSCs) Cells were cultured in DMEM (with 20% FBS) A: Two bars in a graph indicate the CPDL increase ... shown in pellet formation and toluidine blue staining In this study, we used cUCB-MSCs at 3∼5 passage Generally, increasing the passage number of adult stem cells often leads to a decline in the...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence staining of ASC group; A1-A3: Glial fibrillary acidic protein (GFAP), ... canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose-derived adult stem cells: Transplantation of ASCs in spinal cord ... sufficient to show changes of spinal cord lesions in the chronic phase In vivo MSC studies have demonstrated the cellular fate of cells which integrated into injured spinal cord [33] Recent reports...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... supporting the proteomic results Notably, nine cases of LCNEC including four used for the proteomic experiments Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... Poly(rC)-binding protein 4.31 3.79E-02 1.01 23 16 Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 Page of 13 Table Significant changes in...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... gm) were included in the study Animals were inbred in the experimental animal unit, Faculty of Medicine, Cairo University Rats were maintained according to the standard guidelines of Institutional ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched...
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Stem Cells in Endocrinology - part 3 doc
... mobility of rats with spinal cord injuries when injected intravenously Cord blood cells were observed in the areas of injury of spinal cord but not others and never seen in the control, uninjured ... the umbilical cord is a rich source of stem cells Ende coined the term Berashis cells, meaning “beginning cells, ” to describe the primitive multipotential cells found in human umbilical cord blood ... oxygen deprivation of the brain or in a Parkinson’s disease model in rat 3.2 Umbilical Cord Blood Cells Umbilical cord blood is a rich source of hematopoietic stem/ progenitor cells and has been...
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báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc
... after 14 days of culture Many clinical cord blood expansion trials now incorporate the expansion of http://www.jhoonline.org/content/1/1/19 one unit of cord blood following the infusion of another ... role of Alemtuzumab in preserving primitive CB HSC during ex vivo cord blood expansion Results Evaluation of the quality of CB CD34+ hematopoietic progenitors and stem cells The purity of CD34+ cells ... Expansion of umbilical cord blood for clinical transplantation Curr Stem Cell Res Ther 2007, 2(4):324-335 Hofmeister CC, Zhang J, Knight KL, Le P, Stiff PJ: Ex vivo expansion of umbilical cord blood stem...
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Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps
... graded on a five point scale: = minimal injury, = injury up to 25% of the field, = Page of 11 injury up to 50% of the field, = injury up to 75% of the field, and = diffuse injury [19] Myeloperoxidase ... protein macroarray analysis included inflammatory cytokines of interest, including complement 5a (C5a), the soluble form of intercellular adhesion molecule (sICAM)1, IL-1a, IL-1b, IL-6, IL-16, interferon-inducible ... cytokine profiles Lung homogenates at post-injury day were used for a mouse macroarray experiment to profile various inflammatory cytokine proteins involved in the peak inflammatory reactions induced...
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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf
... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit IL-1β induced activation of degradative pathways in MSCs It must be noted that in this study ... positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will require further detailed investigations In the present study we...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... remained in the circulation, showing a times enrichment of Lin- PCs in the pneumonia lungs Few of these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the ... and sequestration of progenitor cells Increased number of circulating progenitor cells has been shown in tissue injury animal models [26,27] as well as in clinical settings including burn, post-cardiac ... remain constant (as shown in the BM homing study by Szilvassy and colleagues [38]), increasing the number of infused progenitor cells will increase the number of these cells that home to injured...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...
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In vitro differentiation of stem cells towards islet like cells
... Mesenchymal stem cells of different origins were shown to differentiate into insulin producing cells under appropriate conditions This study assessed the potential of umbilical cord lining stem cells ... types of cells in the body There are three main types of stem cells: embryonic stem cells isolated from the inner cell mass of blastocysts, neonatal stem cells isolated from umbilical cord, and ... differentiation of amnion-derived stem cells into insulin producing cells Wei et al demonstrated the expression of insulin at transcript level after culturing the amnion-derived stem cells with nicotinamide...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... MA, USA) Binding of all of the antibodies was detected using an ECL detection system (iNtRON Biotechnology, Seoul, Korea) in accordance with the manufacturer’s instructions The intensity of the ... shown that interactions between KCs and endotoxin comprise the initiating event leading to hepatotoxicity in liver injury, including endotoxemia and ischemia ⁄ reperfusion injury [14] In our studies, ... polyclonal anti-phosphoserine ⁄ threonine serum (Abcam) as the primary antibody Binding of all of the antibodies was detected using an ECL detection system (iNtRON Biotechnology) in accordance with the...
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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot
... differentiation of embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells ... cap-binding factor The binding of maskin to eIF4E precludes the binding of eIF4G to eIF4E, thus inhibiting the formation of the translation initiation complex Following stimulation, the kinase ... within this reservoir of self-generating stem cells emerge, at intervals, groups of cells with a distinct morphology called A1 spermatogonia, which mark the beginning of spermatogenesis Each of...
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