3 image acquisition and protocols
Methods in molecular biology vol 1531 type 3 secretion systems methods and protocols
... laws and regulations and therefore free for general use The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate ... translocon proteins YopB and YopD [5154], and the second as a general regulator of type III substrate synthesis and secretion [5559] Interactions between LcrH and the YopB and YopD translocators ... Olson and Deborah M Anderson 10 Measurement of Effector Protein Translocation Using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies Sara Schesser Bartra and...
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Tài liệu LESSON 3: PORTS AND PROTOCOLS doc
... networks - IP addresses - ports and protocols LESSON – PORTS AND PROTOCOLS 3.2 Basic concepts of networks 3.2.1 Devices In order to understand the explanation of protocols and ports, it is necessary ... Pete Herzog, ISECOM LESSON – PORTS AND PROTOCOLS 3.1 Introduction The text and exercises in this lesson try to impart a basic understanding of the ports and protocols in current use, as well as ... LESSON – PORTS AND PROTOCOLS “License for Use” Information The following lessons and workbooks are open and publicly available under the following terms and conditions of ISECOM: All...
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E-Marketing, 3rd editionJudy Strauss, Adel I. El-Ansary, and Raymond FrostChapter 3: The potx
... environmental and SWOT analyses Review the existing marketing plan and any other information that can be obtained about the company and its brands Review the firm’s e-business objectives, strategies, and ... customers to a Web site and to interact with brick -and- mortar customers Firms use Web pages and e-mail to: - Communicate with their target markets and business partners, - Build brand images, - Create ... psychographic, and past behavior toward the descriptors help firms identify potentially attractive markets Allows the company to select its target market and understand its characteristics, behavior, and...
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Internet Security Cryptographic Principles, Algorithms and Protocols - Chapter 3 ppt
... K2 , and K3 The register contents C0 (left) and D0 (right) are computed using Table 3.1: C0 = bcd1a45 D0 = d22e87f Using Table 3.2, the blocks C1 and D1 are obtained from the block C0 and D0 ... blocks P1 , P2 and P3 , and IV are given as: P1 = 0x317f2147a6d50c38 P2 = 0xc6115733248f702e P3 = 0x1370f341da552d79 and IV = 0x714289e53306f2e1 Assume that three keys K1, K2 and K3 used in this ... subkeys’ third and second encryption subkeys For rounds and 9, the second and third decryption subkeys are equal to the additive inverse modulo 216 of Table 3.12 IDEA encryption and decryption...
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Satellite networking principles and protocols phần 3 pot
... network protocols and new services and applications Introduction 51 1.17 Convergence of network technologies and protocols The convergence is the natural progression of technologies pushing and ... development of common standards through an ITU-T and ISO/IEC joint committee The established MPEG2 standard was adopted in DVB for the source coding of audio and video information and for multiplexing ... raw data and uncompressed video and audio, where the data can be, for example, teletext and/ or subtitling information and graphical information such as logos Each of the video, audio and programme-related...
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Antibody Phage Display Methods and Protocols - part 3 ppsx
... of VL and VH repertoires, and their random pairing as Fabs into a phage-display vector The library is posiFrom: Methods in Molecular Biology, vol 178: Antibody Phage Display: Methods and Protocols ... PCR products, digest with EcoRI and XhoI, and gel-purify from a 0.8% TAE gel Combine and purify all second PCR products for VL, digest with SacI and HindIII, and gel-purify from a 0.8% TAE gel ... purification/detection tags, and so on) and adding standard linker sequences if scFvs are to be constructed by overlap extension prior to cloning All primers are shown 5′ to 3′ with standard codes for degeneracy...
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AD HOC NETWORKS Technologies and Protocols phần 3 ppsx
... much lower and more variable bandwidth than wired networks The wireless channel working as a shared medium makes available bandwidth per node even lower So routing protocols should be bandwidth-efficient ... in which the centers of rectangles are the mean values of and throughput and their half widths and half heights are the variance of and throughput‚ respectively These rectangles roughly describe ... and node about the Framework and Mechanisms for Fair Access in IEEE 802.11 47 Figure 2.11 Network configurations with two competing flows existence of each other‚ the handshake between node and...
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Glycoprotein Methods and Protocols - P1
... Glycoprotein Methods and Protocols: The Mucins Edited by: A Corfield © Humana Press Inc., Totowa, NJ Davies and Carlstedt To isolate mucins, the bonds that hold the mucous gel together and those that ... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; antibodies and lectins or coating methods such as the glycan detection method and...
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Glycoprotein Methods and Protocols - P2
... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... microwave oven and set power on “high” and heat the solution so that it boils for Transfer sections to fresh antigen retrieval solution and repeat process Remove from microwave oven and permit sections...
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Glycoprotein Methods and Protocols - P3
... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... Glycoprotein Methods and Protocols: The Mucins (Corfield, T ed.), Humana, Totowa, NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, ... using a standard curve fitted using an appropriate line of best fit; programs are available to interface with microplate readers and isotope counters that store data and compute standard curves...
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Glycoprotein Methods and Protocols - P4
... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus ... adherent to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents Clin Sci 63, 187–195 Bell, A E., Allen, A., Morris, E R., and Ross-Murphy, S...
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Glycoprotein Methods and Protocols - P5
... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...
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Glycoprotein Methods and Protocols - P6
... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... by agarose gel electrophoresis and Western blots of the gels can be probed with lectins and antibodies Using this methodology, we have shown that with pooling and rechromatography it is possible...
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Glycoprotein Methods and Protocols - P7
... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...
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Glycoprotein Methods and Protocols - P8
... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...
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Glycoprotein Methods and Protocols - P9
... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...
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Glycoprotein Methods and Protocols - P10
... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... and methanol (two times) The efficiency of the cleaning procedure should be tested by repeating steps and Absorbance levels above background indicate that antibody remains bound to the pins and ... Chromatography (see Notes 12–15 and Fig 3) Prepare Sepharose-peptide affinity matrices and pack columns according to the manufacturer’s instructions (see Notes 12 and 13) Equilibrate the columns...
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Glycoprotein Methods and Protocols - P11
... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...
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Glycoprotein Methods and Protocols - P12
... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... oven temperature from 120 to 240°C at 2°C/min Use injector and detector temperatures of 240 and 250°C, respectively 168 168 Michalski and Capon Fig GLC separation of trimethylsilylated deriviatives...
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Glycoprotein Methods and Protocols - P13
... Davies, M J and Hounsell, E F (1998) HPLC and HPAEC of oligosaccharides and glycopeptides, in Glycoscience Protocols (Hounsell, E F., ed.), Humana, Totowa, NJ, pp 79–100 14 Kakehi, K., and Honda, ... (3 × 0.5 cm column) and elute with three column vol of HPLC-grade water Collect the effluent and eluent and then pool (2.5-mL volume) Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade ... 6.0, and load the sample carefully and directly to the center of the column, drop wise (see Note 3) Centrifuge for at 1100g and collect the excluded O-Glycanase O-Linked Chain Release and Fractionation...
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