... an ArchiveGap Exists ManuallyTransmittingLogFilesintheArchiveGaptotheStandbySiteManually Applying LogFilesintheArchiveGaptotheStandby Database Creating a Standby Database ... was to transmit redo data tothestandby destination at the same time the online redo log file was being archived tothe local online redo logfiles This change allows the redo log groups to be ... reestablished, the missing archived redo logfiles (referred to as a gap) are automatically detected by Data Guard, which then automatically transmits the missing archived redo logfilestothe standby...
... individual in 11 aspects of life mapped to domains and sub-domains of the ICF: mobility within the home, mobility outside the home, self-care, looking after belongings, looking after the home, looking ... factors on them [17-19] Participation in life situations, such as looking after others and work, as well as psychological well-being [20], may be of more concern to individuals than the underlying ... are not known Such information would help to establish the extent to which older adults maintain or regain their desired levels of function in everyday life – despite an increasing frequency of...
... harvesting operations changing from non-mechanized logging systems to mechanized logging systems inthe said forestlands Therefore, by improving the mechanized logging operations, thelog products increased ... mechanized logging systems In non-mechanized logging systems, after cutting, felling and delimbing, the logs are cut using manual chainsaws into smaller products such as squared timber, sawlogs and ... with the increase inthe total timber production during the studied period, the squared timber production in percentage declined (Fig 5a), but the percentage of log products increased at the same...
... set of mutants in surface-exposed residues to further identify amino acid side chains involved inthe docking of cytochrome c, or inthe presumed electron entry from cytochrome c tothe CuA center ... Using the cyanide-inhibited enzyme, the reaction sequence is limited tothe transfer of the first two electrons reaching the CuA/heme a redox couple To shift cytochrome c oxidation kinetics into ... on the two further subunits of the native oxidase In focussing on the main interaction domain on subunit II, we introduced additional mutations in exposed residues inthe relevant area above the...
... 1), thereby confirming the crucial role of Asp142 Catalysis in family 18 chitinases (Eur J Biochem 271) 259 In most ChiB structures, Asp142 is clearly defined, being either inthe ÔdownÕ or inthe ... Coccidioides immitis was found to be inthe ÔdownÕ position inthe apoenzyme and inthe ÔupÕ position inthe complex with allosamidin [45], which is analogous to what was found for ChiB [21] ... hydrogen bond from the O6 hydroxy (Fig 2) Asparagine is also able to fulfil this role, explaining the residual activity of the D215N mutant The possibility to interact is obviously lost inthe D215A mutant...
... reversible inhibitor was not able to protect the enzyme against thermal denaturation The increased resistance of the a-FMH-treated protein to thermal denaturation would indicate that the covalent binding ... Summarizing, data shown in Fig reinforce our previous findings on the tautomeric forms of the internal aldimine inthe free holoenzyme [19] and the reaction carried out with the suicide analog a-FMH ... not necessarily have to involve the entrance of any inhibitor into the catalytic site, as the conformational change seems to affect the whole enzyme structure In addition, the present results...
... transient, oxazolinium ion intermediate Inthe AD2 E526A–substrate structure, the N-acetyl oxygen of the )1 sugar faces towards Asp524, which is opposite tothe direction in which it points inthe SmChiB–(NAG)5 ... number of amino acid residues contribute tothe binding of the substrate by hydrogen bonding and ⁄ or hydrophobic interactions Using the ligplot program [31], we investigated the specific interactions ... Glu526 side-chain rotated 55° around v1 relative tothe A-form and was exposed tothe solvent The two oxygen atoms of the Glu526 side-chain inthe A-form were positioned close tothe proximal glycosidic...
... S-adenosylmethionine-dependent protein methylases [9] How the sulfur is transferred into the carboxamide group of glycine inthe peptide chain remains to be shown Neither the functions of the five modifications ... of the histidine inside the coenzyme B-binding site With the methylation, the histidine residue is prevented from forming two alternate nitrogen positions by rotating around the angle v2, due to ... However, inthe MCR from the methano4916 trophic archaea of the ANME-1 cluster, the arginine inthe respective tryptic peptide was not methylated (Table 2) The glutamine (Q400) is not methylated in the...
... 2-aminoquinoline (Ks ¼ 17 lM) and indole3-carboxylic acid (the natural auxin, Ks ¼ 18 lM) These compounds are ordered from gain to loss of binding tothe mutant proteins in Table As shown in Table ... residue inthe initial binding and correct positioning of CA during catalysis A role of N302 in anchoring the side-chain carboxylate of CA is further supported by the enhanced binding of amine substituted ... the indole ring In contrast, the I371A mutation appears to remove the steric constraint existing inthe wild-type CYP73A1 and favours a P2/P1 ratio closer toThe observed effects of both of these...
... as holoenzymes (retaining their FAD prosthetic group) The mutants, in their oxidized state, show the typical spectrum of the FADcontaining avoproteins (line in Fig 2), an extinction coefcient at ... in binding were observed for Y238 mutants with the ligands tested (Table 2) These results indicate that the mode of ligand binding is retained inthe two mutants, and that the alteration of the ... equivalents All the potential values are reported vs the standard hydrogen electrode The data were plotted according to Minnaert [17], in which thelog (oxidized/ semiquinone) or thelog (semiquinone/reduced)...
... From the BW Administrator Workbench 11–11 Arranging Workbooks inthe Enterprise InfoCatalog 11–12 Creating Directories inthe Enterprise InfoCatalog 11–12 Getting the Most ... Self-Defined OIW Information Catalog C–8 Activating the OIW Browser in Excel C–10 Downloading/Refreshing the OIW Information Catalog in Excel C–11 Defining and Fine-tuning ... Users tothe SD DemoCube Channel 11–6 Viewing Data inthe SAP DemoCube 11–7 Using the InfoCatalog .11–10 Accessing the InfoCatalog 11–10 From the BW Main...
... unemployment has been in decline for the past six years Although the factory had to be closed, all the employees were relocated to another factory belonging tothe same company ... night to finish their work, but it is impossible to maintain this for very long and so it is not recommended _ One of the main causes of the increase in inner-city lawlessness is the number ... students to take an English language test before they start their course Companies which cannot adapt to changing situations very often go into decline / decrease The tone / style of the meeting was...
... provided their employees Internet access inthe workplace Forty-six of these companies were inthe service sector, whereas the remaining were inthe manufacturing sector These companies ranged in size ... which determines the importance of the transaction which is then factored into the totality of all other transactions in order to determine the value of the judgment Trust becomes the outcome ... Exploratory Study into the Relevance of Trust inthe Context of Information Systems Technology Doctoral Dissertation, The George Washington University, Washington, DC Lippert, S.K (2002) Contributing...
... resupplied from binding sites on plasma proteins Binding to plasma protein is equivalent to a depot in prolonging the duration of the effect by retarding elimination, whereas the intensity of the effect ... Distribution inthe Body Binding to Plasma Proteins Having entered the blood, drugs may bind tothe protein molecules that are present in abundance, resulting inthe formation of drug-protein complexes ... complexes Protein binding involves primarily albumin and, to a lesser extent, !-globulins and acidic glycoproteins Other plasma proteins (e.g., transcortin, transferrin, thyroxin-binding globulin) serve...
... which these ARE elements cooperate in modulating PAI-2 mRNA stability, REMSA analyses were performed to determine the role of the ARE II and ARE IV sites inthe binding of proteins tothe ‘extended ... using primers SJS137 and SJS138, and cloned into the BglII siteinthe b-globin 3¢ UTR in pTETGLO, to generate pTETGLOEXT.ARE The sequences of the primers used inthe present study are listed in ... protein binding activity tothe ‘extended ARE’ The means by which ARE IV cooperates with ARE II to destabilize mRNA still remains unknown The role of the ARE site was not investigated inthe present...
... growth-inhibitory properties of GIF in AD There have been extensive studies examining the role of GIF in AD, linked to its initial discovery as a factor deficient inthe AD brain There remains no ... protein 43, following cortical cryolesion [21] Hence, evidence gathered to date regarding GIF synthesis in response to traumatic brain injury indicates that GIF may be involved inthe neuroinhibitory ... behind the unique biological activity of GIF remain to be elucidated Subsequent studies have investigated whether GIF acts in a neuroinhibitory manner when administered directly into the brain...
... located inthe vicinity of the active site and sensed by the peptidyl substrate are inuenced, in contrast tothe remote exosites, e.g inthe vicinity of the activation pocket, that affect FX binding, ... between the TF-binding region and the active site and between the TF-binding region and the activation domain, appear to function normally In order to further elucidate the effects of the G372(223)A ... FVIIa, whether free or bound to TF, appear to be conned tothe substrate binding cleft (the 170 loop) inthe active site region and the S1 pocket, whereas the intramolecular connections, for instance...
... 266 nm The typical protein absorption peak of 280 nm shifts to 265 nm if the protein contains a flavin-type cofactor [28] We failed to identify the cofactor of the deaminase from strain 10d because ... provided in excess The enzyme activity inthe crude extract and inthe reaction mixture that used 2-amino-5-carboxymuconic 6-semialdehyde as substrate was measured by monitoring the increase inthe ... analysis, and the determination of released ammonia The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step The product...
... for the binding of CP12 The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes observed for the binding of the substrate when the wild-type recombinant ... essential for the assembly of the GAPDHCP12PRK complex Our results also seem to point out that the S loop, known to be involved inthe cofactor-binding site, may also be essential for the interaction ... involved in protein protein interactions These residues being also conserved among other chloroplast GAPDHs (Fig 1B), we mutated them in either alanine or glutamic acid Kinetic parameters of the R197A...