... slurry A number of factors in¯uence this relative risk At a soil pro®le scale, these factors include the relative die-off rates, the relative strength of attachment to soil and to faecal surfaces ... Laboratory experiments on detachment/entrainment in runoff To evaluate the effect of time of contact with soil on E coli mobility, an intact slab of soil was collected using the technique of Douglas ... drainage = 180 mm, runoff = 37 mm Mean E coli c.f.u mL±1 a Replicate Drainage 10 Runoff Total % of total input 10 mean GMb 6.7 Q 10 1.2 Q 1011 9.3 Q 1010 9.0 Q 1010 127 No runoff No runoff 6.7 Q 10 1.2...
... approaches to the problems of immunization with DNA vaccines Furthermore, using combinations of vaccines and alternative routes of administration will allow researchers to customize vaccination ... with live vectors, thereby necessitating the use of large quantities ofDNA Taking into account these factors we also evaluated both in mice and guinea pigs the use of a new concept in vaccine formulation ... (μg/mg of microspheres) DNA – Protein Endotoxin level (UE/mg) - 100% of DNAv plus TDM-loaded PLGA 50:50 microspheres - 100% of DNAhsp65 plus TDM-loaded PLGA 50:50 microspheres A mixture of PLGA...
... about safety ofDNA vaccines always exists, including potential integration of plasmid into host genome, induction of autoimmune responses or immunologic tolerance, and so on, but DNA vaccines have ... been proved to be low immunogenic Adjuvant addition and increased dose of antigen have to be adopted to increase the immune effect [21,22] Compared with these conventional vaccines, DNAvaccine has ... Subgroup (DNA vaccine) Anti-NP Anti-M1 Unexposed to H1N1 NP DNA 12.5 ± 1.0 Not done M1 DNA Not done 10.0 ± 0.81 NP + M1 DNAs 12.5 ± 0.58 10.5 ± 1.29 NP DNA 22.0 ± 0.57a, M1 DNA NP + M1 DNAs Pre-exposed...
... (saliva) consist mainly of secretory IgA (sIgA) [19] This study is the first, to our knowledge, to describe the long term persistence of IgE anti-Influenza virus antibodies in serum of IgE positive and ... representative blot of subject vaccinated with influenza virus vaccine who had low serum IgE levels (
... This kind of L/C allows the seller to transfer all or part of the proceeds of the original letter of credit to a second beneficiary, usually the ultimate supplier of the goods The letter of credit ... need for the buyer to draw against the standby letter of credit, which supports the obligation Back -to- Back letter of Credit Back -to- back L/C is a type of L/C issued in case of intermediary trade ... Standby letters of credit are commonly used to assure the refund of advance payments; support the obligation of a successful-bidder to accept a contract and to perform under the terms of the contract;...
... type (symptoms of chemical intoxication), type (symptoms developed possibly due to chemical exposure), type (symptoms developed not because of chemical exposure but rather because of psychological ... symptoms that the affected person attributes to exposure to low levels of chemicals A report indicated that the rate of school children with MCS-like symptoms, allergies, particularly to offensive ... upset stomach,” “shortness of breath,” and “wheezing” by the Building Assessment Survey Evaluation study.18) These symptoms are almost identical to those of SBS The prevalence of symptoms is...
... isolated mitochondria Finally, alkylation of mtDNA by mitoDC-81 would be expected to disrupt mitochondrial biogenesis and lead to depletion of mtDNA, but there was no generation of q° or respiratory-deficient ... leads to a millimolar concentration of mitoDC-81 within mitochondria on incubation of cells with high nanomolar to micromolar concentrations of mitoDC-81 This high local concentration of mitoDC-81 ... Uptake of mitoDC-81 by energized mitochondria An ionselective electrode was used to measure the uptake of mitoDC-81 into rat liver mitochondria To calibrate the electrode five stepwise additions of...
... all appear to have a role in DNA regulation, in the context of chromatin stability, and to form part of higher complexes linked to active chromatin The DIDO1 gene has been linked to the split ... factor, TFSII [15], that stimulates RNA polymerase II to transcribe through regions ofDNA The structure of domain III of this elongation factor is also solved structurally [16], as a Zn-binding ... was not observed in cultures of wild-type cells (0 of 140; 0.0%) Although merotelic kinetochore attachment to centromeres is generally considered to be a major cause of lagging chromosomes, they...
... initially been reported to function as transcriptional activators of cold-induced genes such as hns [36] and gyrA, encoding a subunit ofDNA gyrase [37] The ability of CSP to bind to singlestranded ... (similar to state 2) (B) Superpositions of L34 residues from Gln34 to Lys39 involving all backbone atoms (Left) Superposition of two models featuring Glu36w ⁄ Gly37/ torsion angle combinations of state ... A2), of which 65 and 35% can be assigned to hydrophobic and hydrophilic areas, respectively Structural organization of the ligand B Fig Binding of hexathymidine to amphipathic platformsof a...
... DNA fragments Fig Chemical structure of WP631 The carbon atoms at positions 13, 14, 3¢ and 4¢ are indicated in the upper part of the dimer analysis revealed up to six distinct binding modes to ... the reaction stopped after by adding lL of mercaptoethanol The DNA was then precipitated with ethanol in the presence of 0.3 M sodium acetate For both DEPC and permanganate the dried DNA pellets ... presence of lM WP631 shown in Fig (tyrT) and Fig (AG1 and GA1) Only a part of each sequence is shown and is written reading 5¢)3¢ from left to right; the right-hand end corresponds to the bottom of...
... inhibitor (+ STI, 0.2 mgÆmL)1) for 15 To examine a role for duodenase-induced release of a mitogenic factor and generation of conditioned media, duodenase was added to cells for 10 prior to addition ... fibroblasts either to become refractory to mitogens or to enter into S-phase more slowly over the time period examined It remains to be established whether chronic exposure to TNFa and IL-1b would ... Pemberton et al (Eur J Biochem 269) with cell surface receptors or induce secretion of a bioactive molecule to induce DNA synthesis In these experiments, addition of conditioned media to pulmonary...
... E (1990) Regulation of the function of eukaryotic DNA topoisomerase I: analysis of the binding step and of the catalytic constants of topoisomerization as a function ofDNA topology Biochemistry ... preference of human topoisomerase I for supercoiled DNA is not the result of an attraction of the enzyme for bent DNA In a previous study [38], we showed that the topo70DL form of human topoisomerase ... properties of a COOH-terminal truncation of topo70 missing the last 106 amino acids (topo58) was similar to those of topo70 Y723F, but a re-examination of these older data [37] reveals that, similar to...
... overexpression of human PRDX5 in mitochondria of Chinese hamster cells decreases formation of 8-oxoG in mitochondrial DNA [20], but the role of this protein in protecting nuclear DNA from oxidation ... inhibition of PRDX5 by CSE can be explained by a CSE-induced decrease in NRF2 (Fig 3C,D) Role of PRDX5 in suppression ofDNA oxidation (formation of 8-oxoG) To study the potential significance of PRDX5 ... Transcription of noncoding DNA A Kropotov et al agents such as CSE (Fig 5E) and in suppression of cell death induced by menadione (Fig 4C) However, the contribution of PRDX5 to menadione-induced DNA oxidation...
... taken it systematically into account in clinical decision making [3] Therefore it is of importance within the medical curriculum to sensitize students to the impact of QoL and health state valuations ... outcome in terms of time spent in a particular health state and with a particular quality of life [5] Different tools were developed to measure health states or health-related quality of life (HRQL), ... were asked to value up to 15 different health states from a sample of 43 states The participants were given selected cards with the description of the health states These cards had to be ranked...
... quantify the levels of HBV DNA in serum as a marker of viral replicative activity [6] The detection and quantification of HBV DNA is reported to have prognostic value for the outcomes of acute and chronic ... Quantification of HBV DNA may be a more useful measure than HBeAg as genetic variants of HBV may continue to replicate at high level without secreting HBeAg Quantification of HBV DNA can be useful to assess ... methodologies were used to monitor HBV DNA levels [11-14] The introduction of PCR-based methods has resulted in a large increase in the sensitivity of HBV DNA detection and commercialisation of PCR-based...
... score of “poor/so-so” comparedto the mode of “poor” for the non-involved group Principles of designing survey questions to minimize bias The involved group had a mode of “good” comparedto “so-so” ... from “so-so” to “good” for two subject areas: knowledge of “the role of SMEs in the livestock feed sector in Vietnam” and knowledge of “principles of designing survey questions to minimize bias” ... Principles of the economics of supply/value chains 3.21 (3) 2.50 (3) 2.50 (2,3) The role of SMEs in the livestock feed sector in Vietnam 2.83 (3) 2.00 (2) 2.75 (2) Indicators of the competitiveness of...
... comparison to the general population top athletes were more likely to have a higher level of education Regarding asthma, allergies and medical treatment, top athletes were more likely to report doctor’s ... potential confounding variables were dichotomised into: ▪ Smoking: never vs ever ▪ Level of education:
... finding include failure to delete autoreactive T cells during thymic selection or decreased activity of regulatory T cells The aim of this study was to analyze the association of the 1858C/T SNP with ... In addition, the influence of the PTPN22 1858T allele on the clinical course of the disease and its relationship to gender, HLA DRB1, the presence of rheumatoid factor (RF) and anticyclic citrullinated ... genotyping revealed that 30.3% of the patients had zero copies, 42.6% had copy, and 27.1% had copies of the shared epitope Control subjects with no history of inflammatory arthritis were recruited...