... as a template The sequences of the PCR primers used are as follows: 5¢-AGAGAGAATTCATATGTCAGATT TGTTCAG-3¢ for primer 1; 5¢-TTCCATACCACCACCT GCAACTTGAAGCTC-3¢ for primer 2; 5¢-GTTGCAGGT GGTGGTATGGAACAGCATGCT-3¢ ... [44] and an RNase T1 refolding assay [45] For the protease-coupling assay, chymotrypsin was used as a protease and Suc-ALPF-pNA (Wako Pure Chemical Industries, Ltd., Osaka, Japan) was used as a ... GGTGGTATGGAACAGCATGCT-3¢ for primer 3; and 5¢-GGCCACTGGATCCAACTACAGCAATTCTCA-3¢ for primer [the NdeI (primer 1) and BamHI (primer 4) sites are underlined] Primers (forward) and (reverse) were used to amplify...
... K Aoyama, Y Kimura, W Takahashi, and M Toyoda, “Approximation of common fixed points ofa countable family of nonexpansive mappings in a Banach space,” Nonlinear Analysis: Theory, Methods & Applications, ... USA, 1984 15 W Takahashi, Nonlinear Functional Analysis, Fixed Point Theory and Its Applications, Yokohama Publishers, Yokohama, Japan, 2000 16 W Takahashi, Convex Analysis and Approximation of ... resolvents of accretive operators,” Journal of Mathematical Analysis and Applications, vol 104, no 2, pp 546–553, 1984 B Halpern, “Fixed points of nonexpanding maps,” Bulletin of the American Mathematical...
... professional/paraprofessional, and a CBT (Cognitive Behaviour Therapy) rather than educational model [10] Self-management approaches can increase dissemination ofevidence based interventions to large ... has been produced as a hard copy format and as a website and participants are able to choose to use either or both of these formats Both versions contain the same information and resources Support ... addressed before a large scale clinical and cost effectiveness evaluation of this approach Specifically, the trial will provide extensive quantitative and qualitative data on the acceptability of the...
... Incubate for days at 37°C ° DENV E protein Wash and fix cell monolayer Automated data analysis Merged Immunofluorescence Assay Add anti-viral Ab and 2° Ab-FITC & DAPI ° Images captured using automated ... of the positive and negative controls, it takes into account three aspects ofa HTS assay: 1) the dynamic range of assay signal, 2) data variation associated with sample measurement and 3) data ... nuclear translocator Asn asparagine ASPH aspartate beta-hydroxylase BHK baby hamster kidney BSA bovine serum albumin C capsid C-TAD C-terminal trans-activation domain CHK checkpoint kinase CPE cytopathic...
... the pollutants concentration was low in the rain season (from May to October) The data from the local weather station showed that the average annual rainfall and evaporation was 802mm and 2093mm, ... order to make full use of the land advantage to strengthen ecological and scenical effect of ecological system The area was 2000 m2 and hydraulic loading rate was 4cm/d in the free-surface constructed ... adopted to treat a typical village sewage There is the long rain season in local place always accompanied with strong storms at the beginning The stormwater runoff not only brings the large amounts...
... Priorities and hazards for Economies Variable levels of activity and management capability Ships’ ballast water and hull fouling are the most important vectors International shipping, aquaculture ... Framework - Introd uced Marine Pests Phase – Consultancy Identified current management capabilities and approaches Priorities and hazards for APEC Economies Considerations fora Risk Management ... are doing Need to have information on what measures economies have implemented What would be most useful to APEC Economies Explore development of agreed regional strategic measures Management...
... smaller the spreading factor is the fewer codes are available Now imagine what happens if spreading factors that allow high data transmission rates are already used and another user wants to ... gives a good overview on how to store and transport performance data that is already available, but is says nothing about how to get such data or about how to compute and analyse it Measurement ... related toa certain RACH can be investigated and the results are stored in a special database of the performance measurement system This database is often named the ‘topology module’ It contains...
... The questionnaire was translated to Kiswahili as a language familiar to most Tanzanians Data was gathered by an anonymous questionnaire The knowledge test consisted of 10 items and asked students ... number of victims of sexual violence is considered a reliable number for Dar er Salaam in Tanzania [30] According to the results that adolescents are in danger of forced sex or early debut of having ... Sabalda Page of Leshabari at Muhimbili University for guidance and academic support We would like to thank Dr Sarah E Porter for her editorial assistance Authors’ contributions FM collected data...
... Rank accuracy ranges from to Classification analysis was performed separately for each participant, and the mean rank accuracy was computed over the participants 3.2 Results and Discussion Table ... measure for subsequent analysis The mean PSC data for each word presentation were further normalized to have mean zero and variance one to equate the variation between participants over exemplars ... exemplars with mean rank accuracies close to 70% We also determined the classification accuracies separately for nouns only and phrases only Distinct classifiers were trained Classification accuracies...
... operations of passive to Variant (lb) is a good translation if the sentence can be active transformation (if necessary) or structural transfer related toa parallel situation or to an action going ... non-resultative activities are always translated by a Russian verb in imperfective aspect form, e.g arbeiten 'to work' - > pa6OTaTT~ A contrasting class of verbs (siegen 'to win', erreichen 'to achieve') ... TENSE form already with the help of the dictionary only The MT system VIRTEX is made to translate simple German main clauses into Russian including the decision of appropriate aspect forms for...
... experiments show a dramatically enhanced gas separation performance for C02 removal from natural gas The mixed matrix membranes can also be used in the separation of the following pairs of gases (14): ... coating solution prior to casting and curing 2.6.3 Interlayer Films for Safety Glasses Poly(viny1acetal) (PVAL)based formulations are used as interlayer films for laminated glass, as binders for ... ceramic forming, as binder for ink or paint and as thermally processable photographic materials (36) Important issues are improved waterproofness and the compatibility with a plasticizer For example,...
... employed to achieve a meaningful set of factors The appropriate number of factors to be extracted was determined as a function of the proportion of common variance accounted for, residuals analysis and ... information captured As the exploratory factor analysis indicated that the items loaded onto two factors – PPSM-Global and PPSM-Pain, Page of (page number not for citation purposes) Health and ... high internal consistency, greater than the desirable 70 – the PPSM Total Score had an alpha score of 97, PPSM-Global had an alpha score of 95 and PPSM-Pain had an alpha score of 96 (see Table 1)...
... female, and the mean age was 49.4 ± 11.0 years Self-rated FM severity was at least moderate in 88.1% of patients, and 88.3% reported a duration of FM of at least years since diagnosis Approximately ... predictive of pain [15] Not surprisingly, both pain and sleep are considered core domains essential for evaluation in FM clinical trials [16] A variety of sleep instruments are available for evaluating ... forwarded written confirmation of the diagnosis of FM to the study investigators Individuals were required to have a current pain rating of > on an 11-point numerical rating scale (NRS) to enable...
... CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF 59 (RFHV/KSHV)7 ... A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC .A A C .T CAC G.GT GAC.G C T GAAGGTT.ACCTGT CG .A C C.ACCT CCTAAAAG.TC ORF 60 CA TGTTACTCATTTTCCGGGCC A AGAGGCTCTATT ... 5'-TCTGAATATGTCACATCCGTTCATA-3' 5'-GGCCCGGAAAATGAGTAACA-3' 5'-(6-FAM)-TGATCTGTAGTCCCCATGTGTCC-(BHQ-1)-3' Exon OSM Exon OSM Exon OSM 5'-CCTCGGGCTCAGGAACAAC-3' 5'-GGCCTTCGTGGGCTCAG-3' 5'-(6-FAM)-TACTGCATGGCCCAGCTGCTGGACAA-(BHQ-1)-3'...
... mechanism driven by SEA actuators) when in contact with a human We have analyzed an equivalent mechanical system where a dissipative element, a mechanical damper was placed in parallel toa spring ... Elastic Actuators IEEE Robotics and Automation Magazine 2008, 15(3):60-69 22 Colgate JE, Schenkel G: Passivity ofa class of sampled-data systems: application to haptic interfaces Journal of ... when in contact with a human We have analyzed an equivalent mechanical system where a dissipative element, a mechanical damper, was placed in parallel with a spring in SEA The goal was to derive...
... instruments, but also leave several fundamental questions unanswered First, to date the majority of biomechanical upper-extremity evaluations involve reaching and trajectory tracking performed at a limited ... sensitivity for use as assessment tools fora home rehabilitation as a component within a larger-scale biomechatronic system A key question relates to which of the many viable metrics are most effective ... vertical settings [35-37], and incorporate a larger range of Page of 15 (page number not for citation purposes) Journal of NeuroEngineering and Rehabilitation 2009, 6:15 motion that can be scaled and...
... CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF 59 (RFHV/KSHV)7 ... A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC .A A C .T CAC G.GT GAC.G C T GAAGGTT.ACCTGT CG .A C C.ACCT CCTAAAAG.TC ORF 60 CA TGTTACTCATTTTCCGGGCC A AGAGGCTCTATT ... 5'-TCTGAATATGTCACATCCGTTCATA-3' 5'-GGCCCGGAAAATGAGTAACA-3' 5'-(6-FAM)-TGATCTGTAGTCCCCATGTGTCC-(BHQ-1)-3' Exon OSM Exon OSM Exon OSM 5'-CCTCGGGCTCAGGAACAAC-3' 5'-GGCCTTCGTGGGCTCAG-3' 5'-(6-FAM)-TACTGCATGGCCCAGCTGCTGGACAA-(BHQ-1)-3'...
... limit of detection for viral load was 400 copies/mL An additional plasma sample was stored for each patient Participants found to have a viral load of >1000 copies/mL underwent a genotypic resistance ... Moore, Sundhiya Mandalia, Jessica Oyugi, Rose Naluggya, Ali Taylor, Petra Schaefer, David Thomas, Keith McAdam and all the staff of the Adult Infectious Disease Clinic and the Academic Alliance The ... Univariate and multivariate logistic regression analysis Table summarizes the univariate results for adherence patterns, clinical events and laboratory variables associated with viral failure Odds ratio...
... 85:308-312 Mshana GH, Wamoyi J, Busza J, Zaba B, Changalucha J, Kalluvya S, Urassa M: Barriers to accessing antiretroviral therapy in Kisesa, Tanzania: a qualitative study of early rural referrals to the ... Roura M, Zaba B, Urassa M: From HIV diagnosis to treatment: Implementing a referral system to monitor access to ART in Kisesa Ward Dar es Salaam 2008 [http:// www.tazamaproject.org/TAZAMA_NACP_files.shtml] ... Roura M, Urassa M, Busza J, Athanas V, Zaba B: Doubts, denial and divine intervention: understanding delayed attendance and poor retention rates at a HIV treatment programme in rural Tanzania AIDS...
... hospital staff was ease of access; for the health centre staff the quality of care offered in the staff clinic was said to be the most important factor The most important reasons for not attending ... Our analysis is based on secondary data collected for routine clinical care and, as such, we are only able to report on a limited number of variables We chose this operational research approach ... for Health 2009, 7:55 12 Makombe SD, Jahn A, Tweya H, Chuka S, Yu JK, Hochgesang M, AberleGrasse J, Pasulani O, Schouten EJ, Kamoto K, Harries AD: A national survey of the impact of rapid scale-up...