γ release and cell mediated cytotoxicity by tumor vaccine draining lymph node tvdln cells after vaccination with mgc8 cells and gm csf ifa
Báo cáo sinh học: "Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke" potx
... 4, and B) Significant improvement in neurological function noted in group 3, 4, and compared with group on day 21 after acute IS, and further improvement observed in group compared with group and ... expressions of Bcl-2 and PGC-1a in group than in other groups and markedly lower in group and than in group and 5, but no difference between group and and between group and † vs other groups, ... higher in groups and than in groups and 5, and notably higher in group compared with that in group Better Preservation of Mitochondrial Cytochrome C after Combined Therapy with CS and EPO against...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
... (M/F) p value Cell and plasma isolation Blood samples (10 ml) from both healthy volunteers and asthmatic patients were obtained by venepuncture, and neutrophils (N) were isolated with a density ... diverse forms including spheres (1, and 8), clusters (2, and 7), plates (5 and 6) and reticular forms (9) corresponding to PM10 particles (indicated by numbers 1–5) and the fine fraction (6–9), (Figure ... and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
... [50], who suggested antagonistic interactions between 1O2 and H2O2 with a reduction of 1O2 -mediated cell death and stress signalling response by H2O2 content In contrast, the present condition of ... treatment) and 80 mM sucrose and 10 M atrazine (SA, tolerance treatment) http://www.biomedcentral.com/1471-2229/9/28 Chlorophyll and carotenoid extraction and quantification Pigments were extracted by ... At3g52880 At5g03630 Monodehydroascorbate reductase (MDAR5) Monodehydroascorbate reductase (MDAR3) Monodehydroascorbate reductase (MDAR4) Monodehydroascorbate reductase (MDAR1) Monodehydroascorbate...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... FL1-H Blank Standard growth condition Stressed condition 100 Fig DCF oxidation in cells with and without glucose Rho+ and q0 cells from osteosarcoma (bone), rhabdomyosarcoma (muscle), and lung carcinoma ... bone q0 cells CuZnSOD mRNA (Fig 4) and protein content were decreased (Fig 5), whereas in muscle q0 cells MnSOD decreased in mRNA and protein amount compared with parental cells (Figs and 5) The ... fluorescence compared to q+ cells Bone-derived q0 cells had the highest level of intracellular ROS compared to muscle and lung q0 cells both in standard growth conditions and in stressed conditions...
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Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
... ** % of apoptosis 50 40 30 20 10 B -cells Non-B cells Mean of fluorescence intensity (X100) B 50 Control BLV *** 40 30 20 10 Non-B cells B -cells ROS levels in B cells (x 100) C BLV control 45 40 ... at a density of 106 cells/ ml and incubated for 30 at 37°C with 10 μM of CM-H2DCFDA After h and h of culture, B cells were stained using anti-IgM monoclonal (clone Pig45) and Alexa Fluor 647conjugated ... twice with phosphate-buffered saline (PBS) supplemented with 0.075% EDTA and three time with PBS alone to deplete platelets After estimation of cell viability by trypan blue dye exclusion, 106 cells...
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Báo cáo y học: "Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen specie" pptx
... and the cells were incubated at 37°C for minutes After incubation, cells were stimulated with heat-aggregated IgG (HAIgG) (100 μg/ml) or by heterotypic or homotypic cross-linking of FcγRIIa and/ or ... After incubation, white blood cells were separated by the dextran sedimentation method described above and resuspended to × 10 7cells/ ml for extracellular staining of the cells Monoclonal antibodies ... percentage of cells expressing FcγRIIIa in the RA population in comparison with the control (data not shown); 10% of lymphocytes expressed FcγRIII These were either natural killer cells or a T-lymphocyte...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
... affected by 1O2 and O3 , whereas there is negative correlation between the genes modulated by 1O2 and those modulated by H2O2 (Table 6) This antagonistic transcriptional regulation mediated by O and ... complete list with subcategories is provided in Additional file 1: Table S3 Many of the genes (up-regulated and down-regulated) fell into the Cell Rescue, Interaction with Cell Environment and Interaction ... -80°C Ascorbate and glutathione were extracted and assayed following the method developed by Queval and Noctor [119] ATP and ADP were assayed as previously described [120] Amino acids and sugars were...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... and dead cells after treatment, or not, with MDP (100 lgÆmL)1) for h Cells were washed twice with cold NaCl ⁄ Pi, detached by trypsinization (1· trypsin), rewashed twice with cold NaCl ⁄ Pi and ... lgÆmL)1 MDP for h (Fig 3) (unstimulated cells 2.48 nmol NO ⁄ 106 cells ± 0.29; MDP treated cells 16.99 nmol NO ⁄ 106 cells ± 0.31; P < 0.05) However, stimulation with MB (100 lgÆmL)1) failed to generate ... macrophages After h of adherence, cells were washed twice with cold NaCl ⁄ Pi to remove non-adherent cells; then they were detached by trypsinization, rewashed twice with cold NaCl ⁄ Pi and finally...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
... epithelial cells and also in vascular smooth muscle cells [101,102], and seems to be involved in angiotensin IImediated hypertension [103–105] Nox1, as well as Nox2, forms a complex with p22phox ... epithelial cells of the adult and fetal kidney [117,118] and vascular endothelial cells [91,119], its function remains to be elucidated Nox4 is complexed with p22phox as well [80,82,84], and constitutively ... via its PRR by interacting with Noxa1 and via its SH3 domains by binding to the Nox1 partner p22phox [106,110,111,149] In cells expressing Noxo1 and Noxa1, Nox1 generates superoxide without stimulants...
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Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
... increased 2.7-fold and 3.8-fold after treatment with PMA at concentrations of 10 and 20 μg, respectively (control versus PMA, p ≤ 0.05), and 2.4-fold and 3.7-fold after treatment with SIN-1 at concentrations ... VEGFR-1 and VEGFR-2 mRNA after stimulation with ROS donors The levels of mRNA encoding VEGF and VEGF receptors (VEGFR-1 (flt-1) and VEGFR-2 (KDR, flk-1)) were quantified by real-time RT-PCR After ... 2.6-fold, 10.4-fold and 4.6-fold at PMA concentrations of 5, 10 and 20 μg, respectively Stimulation of cartilage explants with 10 and 20 μg of PMA upregulated VEGF mRNA by 2.3-fold and 4.9-fold, respectively,...
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Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
... of cells with high energy needs, such as osteoblasts and osteoclasts in bone O2 consumption is increased when ascorbic acid is added to force cells to differentiate into osteoblasts, and ATP release ... U/ml penicillin G (Sigma), and 100 µg/ml streptomycin (Sigma) These cells were maintained in a humidified incubator containing 95% air and 5% CO2, kept at 37 degrees Celsius, and subcultured approximately ... Scientific, Pittsburgh, PA) at a density of about x 105 cells/ cm2 The cells were serum-starved for 24 h in α-MEM supplemented with 0.2%FBS prior to flow Cells were subjected to oscillatory fluid shear...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... c: Cells were stimulated by 480 µg/ml Hp11638M extract and the ROS level was 874; d: Cells were stimulated by 960 µg/ml Hp11638M extract and the ROS level was 1334; e: Cells were stimulated by ... SDS–PAGE, and bands were captured Treatments of AGS cells AGS cells in the logarithmic phase were divided into two groups Cells in one group were grown in the medium containing μmol/L DHR-123 and 60 ... 1212 after stimulation with Hp11638M extract or Hp11638 extract (Fig 2) Moreover, the ROS levels in AGS cells treated with 480 µg/ml and 960 µg/ml Hp11638M extract and with 240 µg/ml, 480 µg/ml and...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
... conditions and after reoxygenation Cleaved caspase-3 protein expression in heart and liver under hypoxic (H) conditions (48 h) and after reoxygenation (48R8, 48R24: 48 h of hypoxia, followed by h or ... Investigation of mechanisms of cell death during cell injury in vivo caused by ischemia and reperfusion is of clinical importance, but technically difficult In heart and liver, apoptotic cell death is demonstrated ... higher H2O2 levels For brain and eyes, there was a gradual increase in the amount of H2O2 after hypoxia and reoxygenation In heart, muscle and liver, the H2O2 level dropped after 24 h of reoxygenation...
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Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot
... is detoxified in the mitochondrial matrix by glutathione peroxidase (GPx1; Fig and Eqn 4) and peroxiredoxins III and V (PrxIII and PrxV; Fig and Eqns and 6, respectively; [55]) At least seven ... DNA in mouse and human cells Basic Life Sci 5B, 589–591 Anderson CT & Friedberg EC (1980) The presence of nuclear and mitochondrial uracil-DNA glycosylase in extracts of human KB cells Nucleic ... protect the cell from damage by the OÀ , by preventing per2 oxide -mediated inactivation of Sod1 [59] Interestingly, Sod itself protects GPx from inactivation by OÀ [60] Thus, Sod and GPx may...
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Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot
... spectrum mode X-band EPR spectra were obtained with a Bruker ESP300 spectrometer with peripheral equipment and data handling as previously described [13] The spectra were simulated with the program ... was carried out by comparison with a Cu2+ standard [13] RESULTS AND DISCUSSION the presence of NADH in the crude extract In order to test this hypothesis, French press cell rupture and preparation ... observed with the more gentle osmotic shock protocol described by Tokuda & Unemoto, with NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
... the synergistic cytotoxicity induced by saikosaponins plus cisplatin in Siha cells, A549 cells, and SKOV3 cells Siha cells (A), A549 cells (B), and SKOV3 cells (C) were pretreated with NAC (1 mM) ... accumulation in Siha cells, A549 cells, and SKOV3 cells Siha cells, A549 cells, and SKOV3 cells were treated with saikosaponin-a (10 μM) or saikosaponin-d (2 μM) for 30 respectively and stained with μM of ... synergistic cytotoxicity induced by saikosaponins plus cisplatin in HeLa cells (A) and (B) HeLa cells were pretreated with BHA (100 μM) or NAC (1 mM) for 30 or remained untreated and then treated with...
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Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps
... numbers and percent neutrophils With unchallenged animals the circulating white blood cell count was ± × 106 cells/ ml, and this number was increased by antigen challenge to 18 ± × 106 cells/ ml ... Intensity Intracellular Superoxide and PKC Inhibition Extracellular Superoxide and PKC Inhibition O2- ( mol/min/106 cells) Protein Kinase C (PKC) inhibition and intracellular Oxidative Activity With circulating ... completed to 50 mL with PMN buffer without calcium, and after a second spin at 400 g for 10 at 4°C, the supernatant was discarded The pellet was washed with calcium free PMN buffer and centrifuged...
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Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx
... calcium handling, which is essential for intracellular signaling Lipid peroxides can also damage DNA and proteins Attack of DNA by ROS results in DNA strand breaks As with lipids, O2•– and H2O2 ... of the cell s basic signalling mechanisms Similarly, regulation of extracellular and intracellular levels of O2•– and H2O2 could provide potential for signalling of extracelluar to intracellular ... was abandoned because of the hole and rusted when it was no longer cared for Signs of oxidative changes may simply indicate that molecules or cells have been abandoned by the organism and are...
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Báo cáo y học: "Endogenous TGF-β activation by reactive oxygen species is key to Foxp3 induction in TCR-stimulated and HIV-1-infected human CD4+CD25- T cells" docx
... TCR-stimulated CD4+CD25- T cells CD4+CD25- T cells were labeled with CFSE (2.5 μM) and cultured with anti-CD3 and anti-CD28 for and days Cells were then counter-stained intracellularly with PE-conjugated ... CD4+CD25- T cells TCR and TCR and CD28 stimulation of human CD4+CD25- T cells produced TGF-β and exhibited phosphorylation of Smad2 A CD4+CD25- T cells (1 × 106/ml) were cultured with antiCD3 and anti-CD28 ... ROS with MnTBAP abrogated active TGF-β and reduced CD25+Foxp3+ T cells Neutralization of ROS with MnTBAP abrogated active TGF-β and reduced CD25+Foxp3+ T cells CD4+CD25- T cells were cultured with...
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