β1 competing peptide yigsr inhibits allergen induced asm accumulation in a guinea pig model of chronic allergic asthma
Báo cáo y học: " The Role of Catalase in Pulmonary Fibrosis" pptx
... 10 Day Day Day 14 Day 21 p=0.009 Day Day Figure Lung Catalase in C57BL/6J mice Administration of bleomycin decreases lung catalase (A) Catalase activity (B) Catalase mRNA (C) Catalase mRNA in ... After an intraperitoneal injection of ketamine and xylazine for sedation and anesthesia, 0.05 U of bleomycin (Blenoxane; Nippon Kayaku, Tokyo, Japan) was intratracheally administrated as described ... Sunami R, Sugiyama H, Wang Da-Hong, Kobayashi M, Maeshima Y, Yamasaki Y, Masuoka N, Ogawa N, Kira S, Makino H: Acatalasemia sensitizes renal tubular epithelial cells to apoptosis and exacerbates...
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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt
... participated in protein isolation, suspension bead array assays and created the associated data charts RS peer-reviewed the data interpretations All authors read and approved the final manuscript Acknowledgements ... incubated for hour After washing, 100 μl Streptavidinphycoerythrin was added to each well and incubated for 30 minutes After a final wash, the plate was analyzed using the Luminex 100 analyzer ... allocating technical resources to assist with performing the suspension bead array and antibody array assays and Walter Bobrowski for assisting with imaging of antibody arrays Page of (page number...
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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
... pathways that are activated via the EGFR and regulate cell survival and proliferation include phosphorylation of mitogen activated protein kinases (MAPK) and Akt/PI-3 kinase pathways Activation of the ... concentrations of CSC induce cell death, it appears that activation of pro-apoptotic signals predominates Studies using inhibition of these separate MAPK signaling pathways are needed to delineate ... of oxidants/free radicals in mediating these effects of CSC Activation of ERK1/2, a MAPK involved in cell proliferation and survival, was increased by various concentrations of CSC, and sustained...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx
... strands of the shRNA1 and shRNA2 designed from Caveolin-1 cDNA are GCAAGTGTACGACGCGCAC and AACCAGAAGGGACACACA, respectively As negative controls (scrambled shRNAs), shRNAcontrol1 (TAGCGACTAAACACATCAA), ... for Caveolin-1 and P2X7 of Caveolin-1 shRNA-treated E10 cells revealed a partial loss of colocalization of P2X7, with a remaining low amount of Caveolin-1 protein and an altered intracellular distribution ... Caveolin-1 and P2X7 expresssion in lung cells K Barth et al among ‘raft’ and ‘nonraft’ domains of the plasma membrane Lipid rafts are microdomains of the plasma membrane containing a high...
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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt
... 5'-CCCGGTGTGGCCATTGCTGC-3' 5'-GCACTTTTATCAATTGGCTTAATCAC-3' 5'-AACGAGTCAGGGTACACACAATATATG-3' 5'-CAATGTCACTATAGCTGGGCCTCCTGCAG-3' 5'-CAGTGCTCTTACCCAGATGGA-3' 5'-TCTGATAATCGATGACAGACTTCA-3' 5'-CTGCCTGTGTTTCAATTCACGAAGCT-3' ... 5'-TGAACTGGACTTCTCCCATTTCCGTCTTTT-3' 5'-CCTGGTTTGTTAATTGGATTAACGA-3' 5'-GAGGTGGAGTGTTGCAAAGGTAGT-3' 5'-CCCATACCAACATCCCTGAGCTGTCAA-3' 5'-AGCTCTGCCTTCACTACAGAGACTT-3' 5'-GCTTTTATGGAAACCTTCATGGA-3' ... (TP) TRAM (FP) TRAM (RP) TRAM (TP) Sequence 5'-CCCATTCCGCAGTACTCCATT-3' 5'-TTTCCTTGGGCCATTCCA-3' 5'-CAGTTATCACAAGCTCAAAAGTCTCATGGCCA-3' 5'-TGTGAAGAGTGAGTGGTGCAAGT-3' 5'-ATGGCAGCATCATTGTTCTCAT-3'...
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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx
... to cause inflammation and damage cells Oxidants are thought to play a major role in cell injury induced by tobacco smoke since each puff of tobacco smoke contains approximately 1017 oxidant molecules ... physical defence and increased permeability of the alveolar space and inflammation all of which are common characteristics of COPD particularly emphysema Similar findings to those described above ... changes are a result of a cascade of biochemical changes occurring within the cell, resulting in activation of caspases and DNA fragmentation [11] However, while it is possible to measure apoptosis...
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Báo cáo y học: "Modulation of epithelial sodium channel (ENaC) expression in mouse lung infected with Pseudomonas aeruginosa" pps
... 25 Day Day Day Day post-infection Day 14 Day 10.0 Cells in BAL/ X 105 Cells in BAL/ X 106 Day 14 Macrophages in BAL PMN in BAL Day Day Day post-infection Day Day Day Day post-infection Day 14 7.5 ... Montréal, Montreal, Canada) was entrapped in agar beads, and 50-µl suspensions containing × 105 to × 106 CFU/ml were instilled intratracheally in male mice of resistant (BALB/c) or susceptible (DBA/2, ... and γENaC expression following lung infection with P aeruginosa Pseudomonas embedded in agar beads was administered intratracheally in resistant (BALB/c) and susceptible strains of mice (DBA/2,...
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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt
... normalized values over that observed with untreated cells Amplification primers for the human genes were as follows: BAFF, 5'-TGAAACACCAACTATACAAAAAG-3' and 5'-TCAATTCATCCCCAAAGACAT3'; β-actin, 5'-GCTGTGCTACGTCGCCCT-3' ... cells staining for cytokeratin (a) and cytokeratin 19 (b) and the absence of staining with cytokeratin 20 (c) indicates the specificity of ductal epithelial cell origin The absence of staining for ... Positive staining with cytokeratin (i) and the absence of staining with cytokeratin 20 (j) indicate the ductal epithelial origin of HSG cell lines Negative staining with myeloperoxydase (MPO) (k) and...
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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... Scanner 3000 7G (Affymetrix) GeneSpring GX 11 (Agilent) was used for the normalization, filtering and statistical data analysis of the Affymetrix microarray data The linear data was first summarized ... merged into a gene list for further GO and pathway analysis GO and pathway over-representation analysis as well as further analysis of protein-protein interactions and transcription factor regulation ... imported into the software for pathway analysis of statistically significant genes Real-time quantitative RT-PCR assays Total RNA was isolated using the RNeasy Mini kit (Qiagen) as described The cDNA...
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Báo cáo y học: "Phenotypic alterations in type II alveolar epithelial cells in cell mediated lung inflammation" pdf
... polyclonal goat arat IgM/IgG/IgA was used as phycoerythrin (PE) conjugate For specific staining of sorted AEC II, the lectin Maclura pomifera agglutinin was used Intracellular staining for IFN-γ and IL-2 ... clearly indicate that its cells are metabolically very active with the capacity to modulate a variety of inflammatory processes through the action of an array of receptor-mediated events Upon activation, ... expressed in AEC II upon airway inflammation Gene (functional category) Symbol SPC-HA/TCR-HA/SPC-HA Array1 Array2 Fold change Array1/Array2 Genes associated with cell cycle cyclin D2 transforming growth...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... cells into alveolar air space Migration of labeled donor cells into alveolar air space In pneumonia lungs, the ratio of cells that migrated into alveolar air space was measured The results are ... Harbor, ME) and maintained on a standard laboratory diet and housed in a controlled environment with a 12-hour light/dark cycle in the animal care facility at Jack Bell Research Centre All animal experiments ... migrate into the airspaces to participate in airspace inflammation but the majority of cells remain in the lung tissues where they have the potential, via proliferation and differentiation, to participate...
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Báo cáo khoa học: " Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by Nacylhomoserine lactone quorum sensing signals" ppt
... in each experiment was calculated as fold change, i.e.: (the mean number of intracellular cfu after addition of AHL)/(the mean number of intracellular cfu without addition of AHL), and thereafter ... transit through the gastrointestinal tract of a guinea pig, a rabbit, a cow, five mice, six pigs, or 12 chickens [15] Interestingly, SdiA was activated in mice that were infected with the AHL-producing ... this article as: Nesse et al.: Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by N-acylhomoserine lactone quorum sensing signals Acta Veterinaria Scandinavica 2011...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... PBS Intraperitoneal injections of PBS or cyclophosphamide were administered on days 1, +1, and +3 after viral inoculation Statistical analysis Analysis of numerical data and statistical analyses ... Franchini M, Hefti HP, Odermatt B, O'Keeffe M, Alber G, Glanzmann B, Riesen M, Ackermann M, Suter M: Flt3 ligandtreated neonatal mice have increased innate immunity against intracellular pathogens ... role of natural killer cells in protection of mice against death and corneal scarring following ocular HSV-1 infection Antiviral Res 2000, 45(1):33-45 Slifka MK, Pagarigan RR, Whitton JL: NK markers...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... PBS Intraperitoneal injections of PBS or cyclophosphamide were administered on days 1, +1, and +3 after viral inoculation Statistical analysis Analysis of numerical data and statistical analyses ... Franchini M, Hefti HP, Odermatt B, O'Keeffe M, Alber G, Glanzmann B, Riesen M, Ackermann M, Suter M: Flt3 ligandtreated neonatal mice have increased innate immunity against intracellular pathogens ... role of natural killer cells in protection of mice against death and corneal scarring following ocular HSV-1 infection Antiviral Res 2000, 45(1):33-45 Slifka MK, Pagarigan RR, Whitton JL: NK markers...
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Báo cáo khoa học: "Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL/6 Mice" potx
... using types of antisera against chromogranin A (CGA), serotonin, somatostatin, human pancreatic polypeptide (HPP), glucagon, gastrin and cholecyctokinin (CCK)-8 Materials and Methods Five adult ... GIT of various vertebrates including rodents Moreover, there is a great deal of data regarding the gastrointestinal endocrine 15, 16 cells in mouse strains Gastrointestinal endocrine cells are ... of this family are found in the secretory granules of a broad spectrum of amine and peptide- producing cells of the adrenal medulla and gastrointestinal endocrine system, and in some neurons of...
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Báo cáo khoa học: " Immunohistochemical Study of the Endocrine Cells in the Pancreas of the Carp, Cyprinus carpio (Cyprinidae)" docx
... Cyprinus carpio Linnaeus (Cyprinidae) having unique hepatopancreas, were observed using specific antisera against mammalian insulin, glucagon, somatostatin and PP by peroxidase antiperoxidase (PAP) ... glucagon, insulin, motilin and gastric acid [26] and absorption of amino acid, glucose and fatty acid in the gastrointestinal tract [6] Somatostatin-IR cells of the teleostean pancreas have been reported ... study, all four kinds of the IR endocrine cells were detected using antisera against mammalian insulin, glucagon, somatostatin and hPP in the pancreatic islets, pancreatic duct and exocrine regions...
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Báo cáo khoa học: "Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome" ppt
... Princeton Instrument, USA) Three sections per animal were examined and the number of MMC was counted in at least 10 randomly selected fields using an image analyzing software Loss of intracellular granules, ... receptor antagonist effectively reduced the mast cell activatorinduced visceral allodynia [8] In addition, it was also reported that experimental activation of proteinase-activated Role of mucosal mast ... rats at 30 days post-nematode infection [18] and the increased abdominal response to rectal distension in stressed rats [13] It is interesting that about a half of MMC is in the degranulated state...
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Báo cáo y học: " Immunohistochemical Characteristics of Bone Forming Cells in Pleomorphic Adenoma" ppt
... myoepithelial cells, appearing in the outer layer Some lumens had eosinophilic material [Fig (2)] In the area of so-called stromal tissue, spindle-shaped cells proliferated in close or in coarse, and ... Y, Sakuma Y, Yokozawa S, Uchida M, Fujita H, Nonaka H A case of pleomorphic adenoma with remarkable bone formation Jpn J Oral Surg 2003; 49: 272-275 Shigeishi H, Hayashi K, Takata T, Kuniyasu ... Ishikawa T, Yasui W Pleomorphic adenoma of the parotid gland with extensive bone formation Pathol Int 2001; 51: 883-886 Thackray AC, Lucas RB Tumors of the major salivary glands Washington DC: Armed...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... therapeutic vaccines for allergic diseases World Health Organization American Academy of Allergy, Asthma and Immunology Ann Allergy Asthma Immunol 1998;81:401–5 13 Durham SR, Walker SM, Varga EM, ... 38 Sakaguchi S, Sakaguchi N, Asano M, et al Immunologic selftolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25) Breakdown of a single mechanism of selftolerance ... immunotherapy inhibits allergen- induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon-c J Allergy Clin...
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Báo cáo y học: "Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis" doc
... Makino H: Expression of interleukin12 in synovial tissue from patients with rheumatoid arthritis Arthritis Rheum 1998, 41:306-314 Nakamura K, Okamura H, Wada M, Nagata K, Tamura T: Endotoxin -induced ... J Clin Invest 1999, 104:1393-1401 Yamamura M, Kawashima M, Taniai M, Yamauchi H, Tanimoto T, Kurimoto M, Morita Y, Ohmoto Y, Makino H: Interferon-gammainducing activity of interleukin-18 in the ... Tomura M, Mu J, Hamaoka T, Nakanishi K, Clark S, Kurimoto M, Okamura H, Fujiwara H: A mechanism underlying synergy between IL-12 and IFN-gamma-inducing factor in enhanced production of IFN-gamma...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25