β lapachone treatment induces apoptosis of the endocardium and erythrocytes which in turn affect heart morphogenetic development and function
Báo cáo y học: " Functional cooperation of of IL-1b and RGS4 in the brachial plexus avulsion mediated brain reorganizatio" pps
... signaling, the emerging picture of G protein signaling (RGS) proteins reveals a highly diverse, multifunctional signaling network, which could permit fine-tuning of its interaction with cytokines [13-15] ... avulsion on the brain reorganization After nerve injury, coordinated tissue remodeling and amplified the mounting cellular response in cortex, brainstem and spinal cord was efficiently initiated and ... element-binding protein; GAP: GTPase activating protein; IL-1b: interleukin 1-b; IL-6: interleukin-6; PKACb: protein kinase A catalytic subunit b; RGS4: regulator of G protein signaling Authors’...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... change in the placebo control Use of Q-PCR using RA and OA synovia To determine whether the Q-PCR technique can detect differences in cytokine gene expression in OA and RA synovia, nine RA and 13 ... in the target tissue in RA, the synovium, which is a primary source of cytokines and effector molecules The first serial biopsy studies to assess the effect of therapeutic agents in RA used in ... in which the fluorescence intensity of each sample exceeds a defined threshold is the C(t) For a given set of primers and template, differences in the C(t) correlate with the amount of starting...
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Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
... ces 003 rof C 27 ta noisnetxe lanif dna ces 09 rof C 27 pmet noisnetxe ,ces 54 rof C 06 pmet gnilaenna ,ces 54 rof C 49 ta noitarutaned ,ces 06 rof C 49 ta noitarutaned laitini ,sgniwollof eht ... rof nitca -β dna 1H2PYC ,A3PYC fo tcudorp RCP lm siserohportcele leg esoraga %1 no nur erew selpmas eht ,nitca -β dna 1H2PYC ,A3PYC fo noisserpxe eneg eht yfitnauq oT suoiverp sa emas si RCP rof ... saw eneg 1H2PYC dna A3PYC rof oitar noisserpxe evitaleR denimreted saw )adanaC ,satnemreF( reluR eneG reddal AND bK dradnatS ot ecnerefer htiw nitca -β dna 1H2PYC ,A3PYC rof ytitnauq fo noitatitnauq...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... identify the HSV type in a population of symptomatic and asymptomatic patients attending a GUM clinic in The Gambia The possible role of other co-infections in the secretion of HSV in CVL fluid and ... effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The discrimination of ... relating to sensitivity of the HSV-1 and -2 probe relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in the...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... identify the HSV type in a population of symptomatic and asymptomatic patients attending a GUM clinic in The Gambia The possible role of other co-infections in the secretion of HSV in CVL fluid and ... effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The discrimination of ... relating to sensitivity of the HSV-1 and -2 probe relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in the...
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Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot
... would reduce the cost of performing the reaction thus making the assays more cost effective and increasing the accessibility of the assay to the application of clinical samples in general While ... types of HPV represented in an FDA approved vaccine, types 6,11,16, and 18 Page 16 of 17 The ability of the assay to distinguish between these types of HPV in a complex mixture of DNA and in the ... region of known HPVs and should allow for detection of any of these types The second qPCR assay targets the coding region of the hypervariable loop V of the L1 gene of HPV6, 11, 16 and 18 These...
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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
... Letters indicate the amino acid in the wild-type receptor The asterisk (*) and double asterisk (**) indicate deletions resulting in a gain of function in hyperfunctioning thyroid adenomas [7] The ... patients and that both Th1 and Th2 cytokine profiles were present in their T cell lines [19] Other reports showed predominance of CD8+ T cells in the orbit with either inconsistent cytokine profiles, ... controversial and the study of the inflammatory processes and cytokine profiling in human tissue samples fraught with difficulties as highlighted in the earlier sections Knowledge of the events in the...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
... 0.005% of the total bacteria present in the biofilm, and their cell number increased during fall (Fig 3) Fig - Seasonal changes in the abundance ratio of microcystin-degrading bacteria in the biofilm ... bacteria The number of cells increased in fall, and the number of microcystin-degrading bacteria increased with the increase in the concentration of phytoplankton By using methods described in this ... present in the biofilm of the practical biological treatment facility throughout the year, and the abundance ratio of the microcystin-degrading bacteria increased to approximately 0.005% of the total...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... maintained 107 copies/g level at weeks postinoculation The copies of the liver, spleen and thymus were more than other tissues in gC DNA vaccine-inoculated ducks, while the copies of the duodenum and ... (1-8), as indicated in the x-axis, whereas the corresponding Ct values are presented on the y-axis The correlation coefficient and the slope value of the regression curve were calculated and indicated ... the copies of most tissues (other than kidney) reached a peak at hours postinoculation in AHV-1 Cha strain-infected ducks The concentration of nucleic acid in DNA vaccine-inoculated ducks maintained...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... S100P, and RAB25, these genes may be significant in the classification of the four subtypes Three SC cell lines were close to each other in PC1, PC2, and PC3 As the presence of subclasses in AD and ... with the gene expression data (Figure 1) In immunostained tumor tissues, AD cells showed immunostaining of S100P in the cytoplasm and the nucleus, while SQ cells showed immunostaining of RAB25 in ... SLCO4A1, STMN1, and TGM2) in the eight test cell lines, with the loading number of components 1, 2, and (PC1, PC2, and PC3; dimensions) shown in Figure 2(A) Using the same frame, the four subtypes...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... expression of uPA are causally involved in tumor progression, the understanding of these underlying intracellular events is of importance In the present study, we explored the signaling pathway linking ... of E-cadherin function induces uPA S Kleiner et al A Role of PI3K in Decma-induced Erk activation The partial suppression of the Decma-induced Erk phosphorylation by Wortmannin suggests the involvement ... leading to endocytosis of the E-cadherin complex Accordingly, in the course of Decma-induced disruption of cell–cell adhesion, Src activation was necessary for the initiation of the signaling...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... expression of genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and ... reverse transcribed and amplified by qRT-PCR in the presence of primers and probes specific for the indicated genes and β- actin house keeping gene (panels A-F) Cytokine and chemokine gene expression ... responses in large groups of vaccinated individuals On the other hand, the power of this method as discovery tool should also be underlined Our data document for the first time that in vaccinated individuals...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... out the YF experiments MM carried out the SARS experiments WS participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... out the YF experiments MM carried out the SARS experiments WS participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and...
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Báo cáo khoa học: "Effects of Benzo[a]pyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells" doc
... at a 37℃ in a humidified incubator in an atmosphere of 5% CO2 for 24 h The various chemicals employed in these investigations were solved in DMSO and the final vehicle concentration in culture ... TCDD, in 96 well tissue culture plate and then incubated at 37℃ in a humidified incubator in an atmosphere of 5% CO2 Following incubation for h, cell proliferation was determined using the MTT ... cycles of 30 s at 94℃, 30 s at 60℃ and 90 s at 72℃ for β- actin, IL- 1β and TNF α, 25cycles of 30 s at 94℃, 30 s at 56℃ and 30 s at 72℃ for IL-6 and IFNγ The sequences of primers used are shown in...
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Báo cáo y học: "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte" pptx
... for ensuring ongoing homeostatic maintenance of cartilage and for bringing about repair to damaged cartilage Maintaining integrity of the nuclear material is critical, and the repair of damaged ... resilience to the tissue were used as measures of chondrocyte function Co-administration of TNF-α and EGF in the present study resulted in a marked increase in the proportion of elongated cells and an ... progression of cartilage degeneration High levels of TNF-α are detected in the synovial lining of rheumatic joints and in chondrocytes of osteoarthritic joints [8] TNF-α promotes further expression of...
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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
... Journal of Surgical Oncology 2008, 6:56 The main structure of E-cadherin catenin complex consists of transmembrane E-cadherin, cytoplasmic proteins, called catenins (α-, β- and γ-catenin), and actin ... filament β- catenin and γ-catenin share approximately 65% sequence homology and bind directly to the cytoplasmic tail of E-cadherin in a mutually exclusive manner α-catenin then links the bound β- or ... cut-offs for positivity and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... phase of infection (i.e and hours post-infection), there was an induction of pro-inflammatory cytokines/chemokines At hours post-H5N1/ 2004 infection, there were 2-5 folds increase in the expression ... hours postinfection At hours post-infection, the level of previously elevated cytokines/chemokines still remained at several folds of induction In contrast to the prominent induction of cytokines/chemokines ... mechanism of cytokine/chemokine biosynthesis may be interrupted by the viral components Cytokines and chemokines generally function in an autocrine (on the producing cell itself) or paracrine (on...
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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc
... studies, and drafted the manuscript CZha participated in the design of the study and performed the statistical analysis SC conceived of the study, and participated in its design and coordination ... analyzed with the Rotor-Gene software Sensitivity of the real-time PCR To determine the detection limit and efficiency of the assay, recombinant plasmid of standard DNA was used as a template and was ... authors read and approved the final manuscript Author details Laboratory of Marine Genetics and Breeding (MGB), Ocean University of China, Qingdao 266003, China 2China Animal Health and Epidemiology...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... pathways involved in both modes of reproduction The identification of genes involved in apomictic development will open the possibility of controlling the expression of this trait and engineering ... interest of controlling the process of cloning through seeds The occurrence of both apomictic and sexual reproduction within Brachiaria makes it an interesting system for understanding the molecular ... after exclusion of the least stable gene, BbrizTUB, there was a decrease in the M value of all the other genes and also a change in the M values of the unstable genes, BbrizGDP and BbrizUBI (Figure...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu