“The Sterile Insect Release Method and Other Genetic Control Strategies”. Rechcigl.“Biological and biotechnological control[r]
(1)GROUP:
DOAN THIEN THANH
VIETNAM NATIONAL UNIVERSITY HO CHI MINH CITY- INTERNATIONAL UNIVERSITY BIOTECHNOLOGY DEPARTMENT
APPLIED ENTOMOLOGY
(2)OUTLINE
1. What is genetic insect control?
Definition Purposes
Advantages and disadvantages
How?
Factors affect on genetic insect control
2. Sterile insect technique – SIT.
(3)(4)Definition
Basic principle: utilize factors which will
lead to reproductive failure
“The use of any condition of treatment that
can reduce the reproductive potential of
(5)Purposes
• Control the growth and development of insect.
• Regulation insect population.
Control pest infestation
Protecting commercial crops
Protect human health from harmful insects (like
mosquitoes)
(6)Advantages and disadvantages
ADVANTAGES DISADVANTAGES
•Friendly with environment.
•Not harmful for
beneficial insect in the same field.
•Not harmful for human health.
•Knowledgeable.
•Require well trained investigators.
•Can not be applied
(7)How?
Based on the induction of dominant lethal mutations in the sperm:
By ionizing radiations or chemical
sterilants
By physical methods
(8)Factors affect on genetic insect control
• Natural density levels. • Cost of rearing.
• Releasing the number of insects required.
• Degree of annoyance or damaged causes by
(9)(10)Sterile insect technique (SIT)
• 1950s - Dr Raymond C Bushland and Dr
Edward F Knipling (Received the 1992 World Food Prize)
• Most widely - most successful.
• Sterile male technique or sterile insect release
method
• Against insect disease vector.
• Suit for insect species that mate only once or
if there is multiple mating, sperm from the
(11)How?
Principle:
Sterile insects + “wild” insects infertile eggs.
How frequently?
(12)(13)Applications
• Pink Bollworm (Pectinophora gossypiella
Saunders)/USA (California)
• Control Culex quinquefasciatus in Sea Horse
Key, Florida coast
• Screwworm fly /USA, Mexico, Libya.
• Mediterranean Fruit Fly (Ceratitis capitata
(14)(15)Advantages/ disadvantages
ADVANTAGES DISADVANTAGES
•Hight cost initial but the control of vector species in an area with a low
population density of the species may be
comparatively
•Saving money in control of year to year where
species can be
•Repeated treatment is required to exterminate the population
•Sex separation is difficult
•Radiation treatment effects health male
(16)Other Genetic Approaches to Insect Control
Inherited sterility
(17)Inherited sterility
Referred to as delayed sterility, F1 sterility,
partial sterility…
Suiting for insects that contain polycentric
chromosomes
Involving the transmission of aberrant
(18)Inherited sterility
2 ways:
Radiation therapy break chromosomes
sterility
Culturing stocks of the target species which
contains homozygous translocations (depend on number and size of the translocations
(19)(20)Chromosomal translocations in an F1 male whose
(21)Inherited sterility- advantages
enhanced reproductive competitiveness of
the partially sterilized individuals compared to the fully sterilized individual used in SIT
The production have higher numbers than
(22)Cytoplasmic incompatibility
• Between insects of the same species with
different Wolbachia infection status
• Sterility is due to a cytoplasmic factor
transmitted through the egg → kill the sperm of the incompatible male after its entry into the
egg
Either unidirectional or bidirectional:
Unidirectional CI : an infected male mates with an uninfected female
Bidirectional CI : in mating between infected
(23)Cytoplasmic incompatibility
• Achieving by mass production
(24)(25)Other genetic approaches
• Principle:
Release not sterile insects produce
sex-ratio distorters (SRDs) meiotic drive
decline population.
• SRDs: heritable elements > modify the sex
ratio > promote transmission
(26)Example
Drosophila melanogaster
D willistoni X ♀ D melanogaster ♂
F1 primarily ♀
D melanogaster X rare F1 ♀ ♂ BC1 primarily ♀
(27)References
• Alan C Bartlett and Robert T Staten USDA, ARS, Western
Cotton Research Laboratory, APHIS, PPQ, Phoenix Methods Development Center Phoenix, AZ 85040 “The Sterile Insect Release Method and Other Genetic Control Strategies”
From http://ipmworld.umn.edu/chapters/bartlett.htm.
• Nancy A Rechcigl.“Biological and biotechnological control
of insect pests” Published by CRC Press, 1999
• Sex ratio- concepts and research methods Cambridge
University Press.
• George Davidson Genetic control of insect pests Published
by Academic Press, 1974 Original from the University of California
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