Thử nghiệm lên men rượu vang bưởi bằng nấm men saccharomyces cerevisiae

T R U ONG DAI HOC AN GIANG KHOA NONG NGHIEP & TAI NGUYEN T H I E N NHIEN BO MON CONG NGHE T H I / C PHAM NGUYEN THI MY ANH DH10TP THtT N G H I E M L E N M E N R U O U V A N G BU'dl B A N G N A M M E N SACCHAROMYCES CEREVISIAE CO DINH T R E N V O B l f d l BAO CAO KHOA LUAN T O T NGHIEP DAI HOC Nganh hpc: Cong Nghe Thirc Phhm Can bo hirdng dan: ThS Ho Thi Ngan Ha KHOA HOC 2009 - 2013 TRUQNG DAI HQC AN GIANG THU VIEN An Giang, Thang 2013 TRU'OfNG DAI HOC AN GIANG KHOA NONG NGHIEP & TAI NGUYEN T H I E N NHIEN BO MON CONG NGHE T H l / C PHAM NGUYEN THI M Y ANH DH10TP THU* N G H I E M L E N M E N R U U V A N G BU'dl BANG N A M M E N SACCHAROMYCES CEREVISIAE C O D I N H T R E N V O BU'dl Do sinh vien: NGUYEN THI MY ANH thirc hien va de nap Kfnh trinh hoi dong cham luan van tot nghiep xet duyet Long xuyen, 03 thang nam 2013 GIAO V I E N HUONG DAN ThS Ho Thj Ngan Ha Lbi cam on Trong suot qua trinh thirc hien de tai nhb su tan tinh giup da cua quy thay co va cac ban, de tai tot nghiep da hoan Co dugc ket qua nay, toi xin gui lai cam on den: Co Ho Thi Ngan Ha, ngubi da true tiep huang dan va giup da toi suot thai gian thirc hien de tai Cac thay co b bo mon Cong Nghe Thuc Pham, khoa Nong Nghiep & Tai Nguyen Thien Nhien, Trubng Dai Hoc An Giang da cho toi nhung kien thuc quy bau thoi gian hoc tap tai trubng Can bo thu vien, can bo phbng thi nghiem bo mon Cong Nghe Thuc Pham, khoa Nong Nghiep & Tai Nguyen Thien Nhien, Trubng Dai Hoc An Giang, da tao dieu kien cho toi hoan tot de tai Cubi cung toi xin gui lbi cam on den cac ban sinh vien lbp Cong Nghe Thuc Pham, khoa 10, khoa Nong Nghiep & Tai Nguyen Thien Nhien Trubng Dai Hgc An Giang da dpng vien va giup toi thoi gian thuc hien de tai tai phbng thi nghiem Toi xin chan cam on! Sinh vien thirc hien Nguyen Thj My Anh Muc luc Noi dung Trang Lai cam an i Muc luc ii Danh sach bang v Danh sach hinh viii Tom lirgc x Chirong Giai thieu 1.1 Dat van de 1.2 Muc tieu nghien cuu 1.3 Npi dung nghien cuu Chirang Lirac khao tai lieu 2.1 Giai thieu ve nguyen lieu 2.1.1 Buai 2 2.1.1.1 Giai thieu 2.1.1.2 Thanh phan hoa hoc 2.1.1.3 Cong dung cua qua bubi 2.1.2 Nam men Saccharomyces cerevisiae 2.1.2.1 Hinh dang te bao nam men 2.1.2.2 Kich thuoc te bao nam men 2.1.2.3 Cau tao te bao nam men 2.1.2.4 Cac hinh thuc ho hap cua nam men 2.1.2.5 Thanh phan hoa hoc va dinh dubng ciia nam men 2.1.2.6 Co che van chuyen cac chat dinh duong vao te bao nam men 2.1.2.7 Sinh duong cua te bao nam men 2.1.2.8 Cac hinh thuc sinh san cua nam men 2.2 Co so khoa hoc va cac yeu to anh hubng den qua trinh len men 10 2.2.1 Co so khoa hoc cua qua trinh len men ruou 10 2.2.2 Cac yeu to anh hudng den len men ruou vang 10 2.3 Ky thuat co dinh te bao 11 2.3.1 Phirong phap co djnh te bao nam men tren be mat chat mang 11 2.3.2 Phirong phap nhdt te bao vao he gel 11 2.4 Cac phu gia (hoa chat) sir dung nghien cuu 11 2.4.1 Duong saccharose 13 2.4.2 Acid citric 13 2.3.3 Natri bisulfit (NaHS0 ) 14 2.5 Cac qua trinh, nguyen ly che bien va bao quan co lien quan 14 2.5.1 Qua trinh chan 14 2.5.2 Qua trinh ep 14 2.5.3 Qua trinh phoi che 15 2.6 Cac nghien cuu trudc day Chirong Phirong tien va phirong phap nghien ciru 3.1 Phuong tien nghien cuu 16 16 16 3.1.1 Dia diem va thoi gian nghien cuu 16 3.1.2 Nguyen lieu 16 3.1.4 Dung cu va thiet bi 16 3.2 Phuong phap nghien cuu 16 3.2.1 Phuong phap thu thap va xu ly so lieu 16 3.2.2 Quy trinh co dinh nam men Saccharomyces cerevisiae tren vo budi 17 3.2.3 Quy trinh len men ruou budi bang nam men co dinh 19 3.3 Noi dung nghien cuu 20 3.3.1 Thi nghiem 1: Khao sat anh hudng cua dp Brix, pH va ty le nam men den qua trinh nhan giong nam men 20 3.3.2 Thi nghiem 2: Khao sat anh hudng cua mat te bao nam men ban dau, ty le vd budi va chieu day vd budi dung co dinh 21 3.3.3 Thi nghiem 3: Khao sat anh hudng cua dp Brix, pH va ty le men giong den qua trinh len men chinh ruou vang budi bang nam men co dinh 22 3.4 Phuong phap phan tich Chirong Ket qua va thao luan 24 25 4.1 Thanh phan cua nguyen lieu 25 4.2 Anh hudng cua Brix, pH va ty le nam men Saccharomyces cerevisiae thuan den qua trinh nhan giong dich budi 26 4.3 Ket qua khao sat anh hudng cua mat te bao ban dau, lugng vd budi va chilu day vd budi den qua trinh co dinh nam men 31 4.4 Anh hudng cua Brix va ty le vd budi da co djnh nam men den qua trinh len men chinh ruou vang budi 34 4.5 Thanh phan san pham 39 Chirong Ket luan va kien nghi 40 5.1 Ket luan 40 5.2 Kien nghi 42 Tai lieu tham khao 43 Phu chuong pc_l Phu chuong A pc_l Phu chuong B pc_2 Phu chuong C pc_7 Phu chuong D pc_9 IV Danh sach bang Bang 1: Thanh phan hoa hoc cua mui budi va vd budi Bang 2: Thanh phan dinh duong cua nam men ( % ) Bang 3: Cac phuong phap phan tfch 25 Bang 4: Thanh phan nguyen lieu dich budi 26 Bang 5: Su thay doi mat te bao nam men djch budi theo thoi gian nhan giong 27 Bang 6: Gia trj mat te bao nam men cue dai d cac gia trj Brix, pH va ty le nam men khac 30 Bang 7: Su thay doi mat te bao nam men co djnh tren vd budi theo thoi gian 31 Bang 8: Gia trj mat te bao nam men co djnh tren vd budi sau gio d cac gia trj mat te bao nam men ban dau, lugng vd budi va chieu day vd budi khac 34 Bang 9: Su thay doi Brix theo thoi gian len men 35 Bang 10: Do san pham d cac gia trj Brix va mat nam men co djnh khac 37 Bang 11: Anh hudng cua Brix ban dau den gia trj cam quan san pham 38 Bang 12: Anh hudng cua mat nam men co djnh den gia trj cam quan san pham 38 Bang 13: Anh hudng cua Brix ban dau va mat nam men co djnh den gia trj cam quan san pham 39 BlUng 14: Thanh ph0n cdla sEn ph0m 40 Bang 15: Bang tra ty le duong nghjch dao pc_4 Bang 16:Bang diem mo ta chi tieu trong, mau sac va mui vj cua rugu pc_7 Bang 17: Bang diem mo ta chi tieu muc ua thich cua rugu vang budi pc_8 Bang 18: Bang ANOVA mat te bao theo Brix, pH va ty le nam mem pc_9 Bang 19: Su thay doi mat te bao nam men theo thoi gian pc_9 Bang 20: Sir tuong tac giua brix, pH va ty le nam men bo sung den mat te bao nhan giong pc_9 Bang21: Sir thay doi mat te bao nam men theo Brix Bang22: Sir thay doi mat te bao theo pH pc_9 pc_10 Bang23: Sir thay doi mat te bao theo ty le nam men V pc_10 Bang 24: Bang ANOVA the hien sir thay doi mat te bao co dinh theo mat te bao ban dau, lugng vo budi va chieu day vd budi pc_9 Bang 25: Su thay doi mat te bao co dinh theo mat te bao ban dau, lugng vd budi va chieu day vd budi pc_10 Bang 26:Su tuong tac giua mat te bao nam men ban dau, ty le vd budi va chieu day vd budi den mat te bao nam men co dinh pc_ Bang 27: Sir thay doi mat te bao co dinh theo mat te bao ban dau pc_ Bang 28: Sir thay doi mat te bao co dinh theo lugng vd budi bo sung pc_ Bang 29: Su thay doi mat te bao co dinh theo chieu day vd budi pc_ Bang 30: Bang ANOVA the hien sir thay doi giua cac mau pc_ Bang 31: Sir thay doi theo ham lugng duong va ty le nam men pc_ Bang 32:Su tuong tac giua Brix va ty le nam men bo sung den sinh ciia san pham pc_ Bang 33: Bang ANOVA the hien diem cam quan ve mau sac theo Brix pc_ Bang 34: Sir thay doi diem cam quan san pham ve mau sac theo Brix pc_ Bang 35: Bang ANOVA the hien diem cam quan ve mau sac theo ty le nam men pc_ Bang 36: Sir thay doi diem cam quan san pham ve mau sac theo ty le nam men pc_ Bang 37: Bang ANOVA the hien diem cam quan ve mui vi theo Brix pc_ Bang 38: Sir thay doi diem cam quan san pham ve mui vi theo Brix pc_ Bang 39: Bang ANOVA the hien diem cam quan ve mui vi theo ty le nam men pc_ Bang 40: Su thay doi diem cam quan san pham ve mui vi theo ty le nam men pc_ Bang 41: Sir thay doi diem cam quan san pham ve mui vi theo ty le nam men pc_ Bang 42: Sir thay doi diem cam quan san pham ve theo Brix pc_ Bang 43: Bang ANOVA the hien diem cam quan ve theo ty le nam men pc_ Bang 44: Su thay doi diem cam quan san pham ve theo ty le nam men pc_ Bang 45: Bang ANOVA the hien diem cam quan ve muc ua thich theo Brix pc_ Bang 46: Su thay doi diem cam quan san pham ve muc ua thich theo Brix pc_ Bang 47: Bang ANOVA the hien diem cam quan ve muc ua thich theo cac ty le nam men pc_ Bang 48: Su thay doi diem cam quan ve mire ua thich theo ty le nam men vi pc_ Bang 49: Bang ANOVA the hien diem cam quan ve mau sac theo Brix va ty le nam men pc_15 Bang 50: Sir thay doi diem cam quan ve mau sac theo Brix va ty le nam men pc_15 Bang 51: Bang ANOVA the hien diem cam quan ve mui vi theo Brix va ty le nam men pc_15 Bang 52: Su thay doi diem cam quan ve mui vj theo Brix va ty le nam men pc_15 Bang 53: Bang ANOVA the hien diem cam quan ve theo Brix va ty le nam men pc_16 Bang 54: Su thay doi diem cam quan ve theo Brix va ty le nam men pc_16 Bang 55: Bang ANOVA the hien diem cam quan ve mire ua thich theo Brix va ty le nam men pc_17 Bang 56: Sir thay doi diem cam quan ve mire ua thich theo dp Brix va ty le nam men bo sung pc_17 vii Danh sach hinh Hinh 1: Buai Nam Roi Hinh 2: Cau tao mot don vi ciia chuoi pectin Hinh 3: Nam men Saccharomyces cerevisiae Hinh 4: Chu ky sinh san cua nam men Saccharomyces 10 Hinh 5: Cau tao hoa hoc cua acid citric 14 Hinh 6: So quy trinh tong quat co djnh nam men tren vo budi 18 Hinh 7: So dd quy trinh len men ruou vang budi bang nam men co djnh 20 Hinh 8: So bo tri thi nghiem 21 Hinh9: So dd bo tri thi nghiem 23 Hinh 10: So dd bo tri thi nghiem 24 Hinh 11: Nguyen lieu Budi Nam Roi 26 Hinh 12: Su tang trudng ciia te bao nam men djch budi theo thoi gian nhan giong d cac gia trj Brix khac 28 Hinh 13: Su tang trudng cua te bao nam men djch budi theo thoi gian nhan giong d cac gia trj pH khac 28 Hinh 14: Su tang trudng ciia te bao nam men djch budi theo thoi gian nhan giong d cac ty le nam men khac 29 Hinh 15: Su thay doi mat te bao nam men co djnh tren vd budi theo thoi gian cd djnh d cac gia trj mat te bao ban dau khac 32 Hinh 16: Su thay doi mat te bao nam men co djnh tren vd budi theo hoi gian cd djnh d cac ty le vd budi khac 33 Hinh 17: Su thay doi mat te bao nam men co djnh tren vd budi theo thoi gian cd djnh d cac chieu day vo budi khac 33 Hinh 18: Su thay doi Brix theo thoi gian len men dieu chinh Brix ban dau khac 36 Hinh 19: Su thay doi Brix theo thoi gian len men dieu chinh mat nam men cd djnh khac 36 Hinh 20: San pham ruou vang budi 20 Hinh 21: Quy trinh cd djnh nam men saccharomyces cerevisiae tren vd budi de nghj 42 Hinh 22: Quy trinh san xuat ruou vang budi de nghj 43 viii te bao nam tren canh ben trai va ben tren ciia dem thi khong thuoc ve dem Tong so te bao dem hinh chu nhat nen tir 100 den 250 te bao Cach tinh ket qua So te bao co mot ml mau ban dau dugc tinh theo cong thirc: „ N= A1000.F h.S Trong do: A: So te bao trung binh mot dem h: Chieu cao ciia buong dem (mm) S: Dien tich cua mot dem (mm") 1000- he so chuyen doi tir mm sang cm F: he so pha loang ciia mau trudc dem B2 Xac dinh ham lugng dudng khu bang phuong phap Lane Eynone Hoa chat - NaOH 30%, 10 % -Pb(CH COO) 30% - Na S0 bao hoa - Metyl xanh % nude - Feling A: CuS0 tinh t h i 69,28g + Nude clt din 1000ml - Feling B: Kali natri tartrate 346g + NaOH lOOg + Nude clt din 1000ml - Phenolphtalein % cdn Tien hanh Lay 10 ml dung dich rugu cho vao binh tarn giac 250ml va 50ml nude cat Trung hda dung dich bang NaOH 10% vdi 2-3 giot phenolphtalein 1% cho den dung djch chuyen tir dd sang vang Khu tap chat bang 7ml Pb(CH3COO)2 De yen phut den thay xuat hien mot ldp chat long suot ben tren ldp can thi coi nhu da khir tap chat xong Ket tua Pb(CH3COO)2 du blng 18-20 ml Na S0 cho tit ca vao binh dinh mire 100ml va them nude den vach Cho vao binh tarn giac: 5ml fehling A + 5ml fehling B, lac deu sau cho vao 15ml djch lgc, dem dun sdi tren bep va quan sat mau cua dung djch Neu mau xanh cua dung djch Fehling bien mat, nghia la dudng du, can pha loang djch lgc va djnh phan lai Neu mau xanh ciia dung djch Fehling chua mat, them tir tir tirng ml dung djch dudng vao binh tarn giac, dun sdi va quan sat cho den cd ket tua mau gach, dung djch khong anh xanh Thu lai bang each nhd mot giot xanh methylen vao dung djch dang sdi thay mat mau xanh trd ve mau gach Dgc the tich va tra bang tinh lugng dudng pc-3 Bang 15: Bang tra ty le dircmg nghjch dao So ml dung dich duong da sir dung I e l J Lirong duong nghich chuyen (mg/ 446 So ml dung dich duong da su aung Luong duong nghich chuyen (mg/ 1UUII1I So ml dung djch duong da su / i ii ii I I OA ZH- 919 Z I 9,9 Luong duong nghjch chuyen (mg/ So ml dung djch duong da su Luong duong nghjch chuyen (mg/ 100ml UUIlg 00m1 560 49 19 44 11 J5 Z9 , Z 44 191 OQ 44 t*t 1R UUIlg ZH-,Z 1 D fi "41 - J 904 R 9QR 9O Z Q7 J J R ZR o9 Z 01 QO 97 fi 144 Q 45 116 11 V Q 7/ ZO z7 o8 R4 109, / 77 / 140 • t6u 114 119,/ 20 254,4 29 177,6 136,6 47 111,4 21 242,2 30 171,7 39 133,3 48 109,2 22 231,8 31 166,3 40 130,1 49 107,1 23 222,2 32 161,2 41 127,1 50 105,1 110,/ Ket qua So tra bang i: HSPL :] 100 Ham lugng dudng khir = (%) Klidi lugng mau 1000 B3 Phuong phap dinh luong vitamin C Nguyen tac Acid ascorbic la hgp chat khong no chua nhom endiol de bi oxi hoa khu thuan nghich, bi pha huy nhanh dudi tac dung ciia chat oxi hoa va ben mdi trudng acid.Vitamin C cd the khu dung djch Iod nen co the djnh lugng vitamin C bang phuong phap chuan vdi dung djch I 0,01 N , vdi chi thj mau la dung djch tinh bdt cho den cd mau xanh Hoa chat - Dung djch HC1 5% - Dung djch hd tinh bdt 1% -Dung djch I 0,01N Tien hanh Lay gam djch ep so ri cho vao binh djnh muc 50 ml vdi ml HC1 5%, cho nude cat vao du 50 ml Lac cho ddng nhat Lay 20 ml djch tir binh djnh mire cho vao erlen dung tich 100 ml, chuan bang dung djch I cd tinh bdt lam chi thj mau cho den mau xanh pc-4 Tinh ket qua Ham lugng vitamin C tinh theo cong thuc: x/„\_V*Vi y°l-~ *0,00088*100 y2*w Trong do: - V: s6 ml dung dich I 0.01 N dung chuan (da trir the tich I trlng) 0,0IN chuan mau - Vj: The tich djch thi nghiem (50ml) - V :The tich lay chuan (20ml) - W: Khoi lugng mau dem xac djnh vitamin C - 0,00088: so gam vitamin C tuong ung vdi ml dung djch I 0,01 N B4 Phuong phap dinh luong acid tong so Nguyen ly Dung dung djch kiem de trung hoa het cac acid mau vdi chat chi thj mau la phenolphatalein den mau hong nhat ben vung Hoa chat -Dung djch NaOH 0,1N - Dung djch phenolphthalein 1% cdn 90° Tien hanh Lay 10 ml djch ep so ri cho vao binh djnh muc 100ml, cho them nude cat vao vira du 100 ml de d l nhan biet diem chuyen mau djch ep so ri chin co mau dd Lac cho ddng nhat Dung pipet lay 25 ml djch mau tir binh djnh muc cho vao binh tarn giac, nhd vai giot phenolphtalein vao va dem chuan Nhd dung djch NaOH 0,1 N tir buret xudng cho den djch thu co mau hong nhat ben vung Tinh ket qua Ham lugng acid toan phan tinh theo cdng thuc sau: X (g/lft)= K* n *(100/25) * (100/v) Trong do: - K: he so loai acid (acid citric K=0,0064) - N: so ml NaOH 0,1N dimg de chuan 25 ml mau thu (ml) - v :the tich mau ban dau lay djnh lugng acid tong (ml) B5 Xac dinh dp ruou Dung binh tarn giac co dung tich 500ml, rot 100ml rugu can thu va 100ml nude cat lac deu cho vao dung cu cat Cho len bep dun cat lay dung 100ml dung djch rugu tai dau dng sinh han Sau dem lam lanh, nhiet rugu cat 20°C, dung rugu ke de do rugu, dgc rugu d nhiet 20°C chinh la ciia rugu vang Phu chirong C: Cac bang diem mo ta danh gia cam quan pc-5 Bang 16: Bang diem mo ta chi tieu trong, mau sac va mui vi ciia san pham rirou vang birdi fi^ni i t ! ueu *;r>ii XX i y, 11Ullg J 4 Do I vll »V1 r •» Trong suot, co it vat the nho A x \ - / x x v- _ , w vy x v x v y x x ^_ vx vy x x x a a x w ax t ax a ax i v x n x vy • T-Tnri rtiir P O nhipn vat tlip tho t V ' l v l 1 L_ , t - t ' 111 1 V Li V Ci L LI L LI Ivy n Due nhieu can lang, co nhieu vat the tho \j Van v a n niif V I U L san o a n nham i j i i a i n khnno i v i i v j i i t ; dat uai Man vano sano rat dap tnmp Mau vang sang dac trung Mau vang sang hoi dac trung Mau vang hoi dam Mau vang dam Mau vang rat sam, san pham khong dat Mui thorn rat dac trung ciia san pham, vi rat hai hoa, hau vi tnt Mui thorn dac trung ciia san pham, vi hai hoa, hau vi tot Mui thorn hoi nong ciia san pham, vi chua hoa hop, hoi gat Mui nong, dang soc, vi kem hai hoa, thoang mui la Mui hang, vi la ro ret Mui la kho chiu, san pham bi hu hong * ci Ll Mui vi bUOl Trona co tuons doi nhieu vat the nho — Mau sac Call V C U l l l J d l l l l at U u v pc-6 L l U l l E l Bang 17: Bang dilm mo ta chi tieu mire ira thich cua riryu vang budi Mire mo ta Diem tirong ung Thich cue Thich rat nhieu Thich vira phai Thich hoi hoi Khong thich khong chan Chan hoi hoi Chan vira phai Chan rat nhieu Chan cue pc-7 Phu chirong D: Ket qua thong ke cac so lieu thi nghiem Dl Ket qua thong ke thi nghiem 1: Ket qua khao sat anh hudng cua dp Brix, pH va ty le nam men den qua trinh nhan giong nam men Bang 18: Bang ANOVA mat te bao theo Brix, pH va ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 48.5727 26 1.86818 31.37 0.00(X) Within groups 3.21552 54 0.0595467 Total (Coir.) 51.7883 80 Bang 19: Su* thay doi mat te bao nam men theo thoi gian Multiple Range Tests for Mat te bao by Mau Mau Count Mean Homogeneous 3.072 X 3.2 XX 19 ; 3.264 XXX 3.36 xxxx 25 3.488 xxxx 3.584 xxxxx 3.648 xxxxx 3 3.672 xxxx 16 ; 3.712 xxxx 22 3.72 xxxx 3.804 11 3.70o V vv XXX 3.968 XXX 4.032 XX 10 4.256 20 4.48 21 4.848 XX 13 4.864 XX 23 4.992 XX 18 5.064 XX 27 5.112 XX 24 5.16 XX 12 5.16 XX 17 5.184 XX 15 ; 5.208 XXX 26 5.376 XX 14 Groups XXX XX X pc-8 Bang 20:Sir tirong tac gitra brix, pH va ty le nam men den mat te bao nhan giong Analysis of Variance for mat Source M A I N EFFECTS A:Do Brix B:pH C:ty le nam men INTERACTIONS AB AC BC ABC RESIDUAL T O T A L (CORRECTED) te bao - Type HI Sums of Squares Mean Square Sum of Squares Df F-Ratio P'-Value 41.1118 15.5499 27.8249 2 20.5559 7.77497 13.9124 12.96 4.90 8.77 0.0000 0.0081 0.0002 7.33799 18.4278 2.46458 2.73351 428.27 541.121 4 270 296 1.8345 4.60695 0.616146 0.341689 1.58619 1.16 2.90 0.39 (i 22 0.3304 0.0223 0.8168 0.9880 Bang 21: Su* thay doi mat dg te bao nam men theo Brix Multiple Range Tests for Mat te bao by Do Brix Method: 95 percent LSD Do Brix ('ount LS Mean LS Sigma Homogeneous Groups 19 27 3.59333 0.0904642 X 25 27 4.49333 0.0904642 22 27 4.77956 0.0904642 X X Bang 22: Su* thay doi mat te bao theo pH Multiple Range Tests for Mat te bao by pH pH Count LS Mean LS Sigma Homogeneous Groups 3.5 27 4.02311 0.0904642 X 4.5 2^ 4.29867 0.0904642 X 27 4.54444 0.0904642 X Bang 23: Su* thay doi mat te bao theo ty le nam men Multiple Range Tests for Mat te bao by Ty le nam men 7Y le nam men ('ount LS Mean LS Sigma Homogeneous Groups 0.2 27 3.76622 0.0904642 X 0.3 27 4.53333 0.0904642 X 0.4 27 4.56667 0.0904642 X D2 Ket qua thong ke thi nghiem 2: ket qua khao sat anh hudng cua mat dp te bao nam men ban dau, ty le vd budi va chieu day vd budi dung cd dinh Bang 24: Bang ANOVA the hien su* thay doi mat te bao co djnh theo mat dg te Mean Square F-Ratio P-Value 26 0.56195 19 42 O.OOOd 1.59181 55 0.0289421 16.2025 81 Source Sum of Squares Between groups 14.6107 Within groups Total (Corr.) pc-9 Bang 25: Su* thay doi mat te bao co dinh theo mat te bao ban dau, liryng vo biroi va chieu day vd birdi Multiple Range Tests for Mat te bao by Mau Mau Count Mean Homogeneous 0.938667 X 1.25867 X ! 1.432 XX 1.44533 XX 3 1.45867 XX 1.49333 XXX 19 1.496 XXX 20 1.57333 XXX 1.58133 XXX 24 1.59 XXX 10 1.59733 XXX 1.60267 XXX 1.64267 xxxx 1.664 xxxxx 22 1.696 xxxxx 27 1.74933 21 1.84267 xxxx 12 1.90667 XXX 25 1.90667 XXX 16 1.92533 XX 26 2.032 X ? 2.06667 X 15 2.088 X 18 2.37067 X 17 2.416 X 23 2.50933 XX 14 Groups xxxx 2.85067 X Bang 26:Su* tirong tac giira mat te bao nam men ban dau, ty le vd birdi va chieu day vd birdi den mat te bao nam men cd djnh Source MAIN E F F E C T S A:Col B:Col_2 C:Col INTERACTIONS AB \C IKABC RESIDUAL TOTAL(CORRECTED) Sum of Squares Pf Mean Square F-Ratio P-Value 5.78192 > > '2 1.947NS 2 2.89096 1.41436 0.973941 172.60 84.44 58.15 0.00(K) 0.0(100 0.933019 0.0704648 1.20697 1.67497 0.904448 15.3484 4 0.233255 0.0176162 0.301742 0.209371 0.016749 13.93 1.05 18.02 12.50 o.oooo 54 80 pc-10 0.0000 0.3893 0.0000 0.0000 Bang 27: Sy thay doi mat te bao co dinh theo mat te bao ban dau Multiple Range Tests for Mat te bao co dinh by Mat te bao ban dau Mat te bao ban dau Count LS Mean LS Sigma Homogeneous Groups 20 27 1.45393 0.0543695 X 60 28 1.80943 0.0534347 40 27 2.10341 0.0543695 X X Bang 28: Sy thay ddi mat te bao co dinh theo liryng vd birdi bd sung Multiple Range Tests for Mat te bao co dinh by Luong vo buoi Luong vo buoi Count LS Mean LS Sigma Homogeneous Groups 10 27 1.54311 0.0543695 X 20 27 1.68474 0.0543695 15 28 1.93892 0.0534347 X X Bang 29: Sy thay doi mat te bao cd djnh theo chieu day vd birdi Multiple Range Tests for Mat te bao co dinh by Chieu day vo buoi Chieu da\ vo buoi ('ount LS Mean LS Sigma Homogeneous Groups 0.5 27 1.61837 0.0543695 y 28 1.75077 0.0534347 X 1.99763 0.0543695 : 0.75 _ X D3: Ket qua thong ke thi nghiem 3: ket qua khao sat anh hudng ciia dp Brix dich budi vd mat dp nam men cd dinh den qua trinh len men chinh nrgu vang budi Source Sum of Squares D Mean Square F-Ratio P-Value Between groups 142.146 15 9.47639 26.76 0.0000 Within groups 11.3333 32 0.354167 Total (Corr.) 153.479 47 f Bang 31: Sy thay ddi dg cdn theo ham lirgng dircmg va ty le nam men Multiple Range Tests for Do by Mau Mau Count Mean Homogeneous Groups 5.33333 X 5.33333 X 3 5.66667 XX 6.33333 X 10 8.0 X 16 8.33333 X 8.66667 XX 15 8.66667 XX 13 9.33333 XX pc-11 9.33333 XX 9.66667 X 12 9.66667 X 14 9.66667 X 9.66667 X 9.66667 X 11 11.0 X Bang 32:Su* tirong tac giua Brix va ty le nam men bo sung den sinh cua san pham Source M A I N EFFECTS A:Do Brix B: Luong nam men INTERACTIONS AB RESIDUAL TOTAL(CORRECTED) Sum of Squares Df Mean Square F-Ratio P'-Value 120.353 3.23756 3 40.1177 1.07919 96.89 2.61 0.0000 16.5568 13.25 153.479 32 47 1.83965 0.414063 4.44 O.IMIOS 0.0687 Bang 33: Bang ANOVA the hien diem cam quan ve mau sac theo Brix Source Sum of Squares Pf Mean Square F-Ratio P'-Value Between groups 11.7634 3.92113 6.18 0.0005 Within groups 139.625 220 0.634659 Total (Corr.) 151.388 223 Bang 34: Su* thay doi diem cam quan san pham ve mau sac theo Brix Multiple Range Tests for Mau sac by Do Brix Do Brix Count Mean Homogeneous Groups 16 56 3.42857 X 25 56 3.76786 X 19 56 3.82143 XX 22 56 4.07143 X Bang 35: Bang ANOVA the hien diem cam quan ve mau sac theo ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 5.62054 1.87351 2.83 0.0004 Within groups 145.768 220 0.662581 Total (Corr.) 151 388 223 Bang 36: Su* thay doi diem cam quan san pham ve mau sac theo ty le nam men Multiple Range Tests for Mau sac by Ty le nam men 7Y le nam men Count Mean Homogeneous Groups 56 3.55357 X 56 3.75 XX 56 3.78571 XX 56 4.0 X pc-12 Bang 37: Bang ANOVA the hien diem cam quan ve mui vi theo Brix Source Sum of Squares Pf Mean Square F-Ratio P-Value Between groups 8.29911 2.76637 5.86 0.0005 Within groups 103.911 220 0.472321 Total (Corr.) 112.21 223 Bang 38: Sy thay doi diem cam quan san pham ve mui vj theo dg Brix Multiple Range Tests for Mui vi by Do Brix Do Brix Count Mean Homogeneous Groups 25 56 3.625 X 19 56 3.75 X 16 56 3.78571 X 2: 56 4.14286 X Bang 39: Bang ANOVA the hien diem cam quan ve mui vj theo ty le nam men Source Sum of Squares Pf Mean Square F-Ratio P'-Value Between groups 6.26339 2.0878 4.34 0.0004 Within groups 105.946 220 0.481575 Total (Corr.) 112.21 223 Bang 40: Sy thay doi diem cam quan san pham ve mui vj theo ty le nam men Multiple Range Tests for Mui vi by Ty le nam men 7v le nam men Count Mean Homogeneous Gr< tups 56 3.58929 X 56 3.78571 XX 56 3.875 56 4.05357 XX X Bang 41: Bang ANOVA the hien diem cam quan ve theo Brix Source Sum of Squares Pf Mean Square F-Ratio P-Value Between groups 7.58929 2.52976 4.31 0.0001 Within groups 129.25 220 0.5875 Total (Corr.) ! J6.839 223 Bang 42: Sy thay doi diem cam quan san pham ve theo Brix Multiple Range Tests for Do by Do Brix Do Brix Count Mean Homogeneous Groups 25 56 3.42857 X 16 56 3.51786 XX 19 56 3.78571 22 56 3.875 XX X pc-13 Bang 43: Bang ANOVA the hien diem cam quan ve theo ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 2.94643 0.982143 1.61 0.0001 Within groups 133.893 220 0.608604 Total (Corr.) 136.839 223 Bang 44: Su* thay doi diem cam quan san pham ve theo ty le nam men Multiple Range Tests for Do by Ty le nam men Ty le nam men Count Mean Homogeneous Groups 56 3.53571 X 56 3.58929 XX 56 3.64286 XX 56 3.83929 X Bang 45: Bang ANOVA the hien diem cam quan ve mire ira thich theo dg Brix Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 18.5134 6.17113 6.71 0.0002 Within groups 202.268 220 0.919399 Total (Corr.) 220.781 223 Bang 46: Su* thay doi diem cam quan san pham ve mire ira thich theo Brix Multiple Range Tests for MDUT by Do Brix Do Brix Count Mean Homogeneous Groups 16 56 6.67857 X 25 56 6.73214 XX 19 56 7.07143 XX 22 56 7.39286 X Bang 47: Bang ANOVA the hien diem cam quan ve mire ira thich theo ty le nam men Source Sum of Squares Pf Mean Square F-Ratio P-Value Between groups 12.0134 4.00446 4.22 0.0003 Within groups 208.768 220 0.948945 Total (Corr.) 220.781 223 Bang 48: Su* thay doi diem cam quan ve mire ira thich theo ty le nam men Multiple Range Tests for MDUT by Ty le nam men 7Y le nam men Count Mean Homogeneous Groups 56 6.76786 X 56 6.82143 X 56 6.92857 X 56 7.35714 X pc-14 Bang 49: Bang ANOVA the hien diem cam quan ve mau sac theo Brix va ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 39.0312 15 2.60208 4.82 0.0000 Within groups 112.357 208 0.540179 Total (Corr.) 151.388 223 Bang 50: Su* thay doi diem cam quan ve mau sac theo Brix va ty le nam men Multiple Range Tests for Mau sac by Mau Method: 95.0 percent LSD Mau Count Mean Homogeneous Groups 14 2.78571 X 14 3.07143 XX 16 14 3.14286 XX 14 3.57143 14 3.71429 XX 14 3.78571 XX 15 14 3.78571 XX 14 3.85714 XXX 14 3.92857 XXX 12 14 4.0 XXX 13 14 4.0 XXX 14 4.07143 XXX 10 14 4.07143 XXX 14 4.07143 :•: xx 14 14 4.14286 XX I 14 4.35714 X XX Bang 51: Bang ANOVA the hien diem cam quan ve mui vi theo Brix va ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 27.2812 15 1.81875 4.45 0.0000 Within groups 84.9286 208 0.40831 Total (Corr.) 112.21 223 Bang 52: Su* thay doi diem cam quan ve miii vi theo Brix va ty le nam men Multiple Range Tests for Mui vi by Mau Method: 95.0 percent LSD Mau ( ount Mean Homogeneous Groups 16 14 3.14286 X 14 3.28571 XX 15 14 3.5 XXX 14 3.5 XXX 14 3.64286 XXX pc-15 14 3.71429 XXXX 12 14 3.78571 XXX 14 14 3.78571 XXX 14 3.92857 XXX 14 3.92857 XXX 14 4.0 XX 13 14 4.07143 XX 14 4.07143 XX 14 4.14286 XX 10 14 4.14286 XX 11 14 4.57143 X Bang 53: Bang ANOVA the hien diem cam quan ve theo Brix va ty le nam men Source Sum of Squares Pf Mean Square F-Ratio P-Value Between groups 21.5536 15 1.4369 2.59 0.0001 Within groups 115.286 208 0.554258 Total (Corr.) 136.839 223 Bang 54: Sy thay doi diem cam quan ve theo Brix va ty le nam men Multiple Range Tests for Do by Mau Method: 95.0 percent LSD Mau Count Mean Homogeneous Groups 16 14 3.21429 X 14 3.28571 XX 15 14 3.35714 XX 14 3.42857 XX 14 14 3.42857 XX 14 3.5 XXX 14 3.64286 XXX 14 3.64286 XXX 14 3.71429 XXX 12 14 3.71429 XXX 14 3.71429 XXX 13 14 3.71429 XXX 10 14 3.71429 XXX 14 3.78571 XX 14 4.0 I 14 4.57143 X X pc-l Bang 55: Bang ANOVA the hien diem cam quan ve mire ira thich theo Brix va ty le nam men Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 39.7098 15 2.64732 3.04 0.0002 Within groups 181.071 208 0.870536 Total (Corr.) 220.781 223 Bang 56: Su* thay doi diem cam quan ve mire ua thich theo Brix va ty le nam men Multiple Range Tests for MDUT by Mau Mau Count Mean Homogeneous Groups 13 14 6.28571 X 14 £ XX A^tO CI 6.42857 14 O.J Y V Y A A A 14 14 6.57143 XXXX 14 6.78571 xxxxx 14 6.85714 xxxxx 14 6.92857 xxxxx 16 14 6.92857 xxxxx 14 7.07143 xxxx 12 14 7.07143 xxxx 15 14 7.14286 XXX 14 7.14286 XXX 10 14 7.14286 XXX 14 7.21429 XX 14 7.28571 X 1 14 8.14286 A X pc-17 ... ruou vang vira ngon lai vira dem lai nhieu lpi ich cho sue khoe Nam men Saccharomyces cerevisiae la chung nam men dugc sir dqng bien qua trinh len men rugu vang. Trong qua trinh san xuat ruou vang, ... men ma ngudi ta chia lam nhieu kilu len men khac Tuy nhien co hai hinh thuc len men chinh la len men yem (ki khi) va len men hieu Len men ruou la qua trinh len men yem vdi su co mat cua nam men, ... dinh nam men saccharomyces cerevisiae tren vo budi la phuong phap nham giu cac te bao nam men tren vd budi Ben canh dd, len men ruou vang budi bang nam men Saccharomyces cerevisiae co djnh tren