BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP HỒ CHÍ MINH ĐỒ ÁN TỐT NGHIỆP SỰ KHÁC BIỆT VỀ DI TRUYỀN CỦA CÁC CHỦNG Aeromonas hydrophila PHÂN LẬP TRÊN CÁ TRA (Pangasianodon hypophthalmus) Ở ĐỒNG BẰNG SƠNG CỬU LONG Ngành: Cơng Nghệ Sinh Học Chun ngành: Công Nghệ Sinh Học Giảng viên hướng dẫn : ThS Nguyễn Thị Hiền Sinh viên thực MSSV: 1051110078 : Nguyễn Trần Trúc Huân Lớp: 10DSH01 TP Hồ Chí Minh, 2014 ĐỒ ÁN TỐT NGHIỆP LỜI CAM ĐOAN Tôi xin cam đoan cơng trình nghiên cứu riêng Các số liệu, kết nêu Luận văn trung thực chưa công bố cơng trình khác Tơi xin cam đoan giúp đỡ cho việc thực Luận văn cảm ơn thông tin trích dẫn Luận văn rõ nguồn gốc Sinh viên thực luận văn Nguyễn Trần Trúc Huân i ĐỒ ÁN TỐT NGHIỆP LỜI CẢM ƠN Em xin chân thành cám ơn: Ba mẹ gia đình tạo điều kiện thuận lợi để em học tập, nghiên cứu hoàn thành tốt luận văn Ban Giám Hiệu trường Đại học Công Nghệ Thành phố Hồ Chí Minh, thầy giáo khoa Công Nghệ Sinh Học- Thực Phẩm- Môi Trường truyền đạt cho em kiến thức, kinh nghiệm quý báu suốt thời gian qua Đặc biệt thầy Phạm Minh Nhựt tạo điều kiện để em thực đề tài ThS Nguyễn Thị Hiền, chị tận tình giúp đỡ, trực tiếp bảo, hướng dẫn em suốt trình làm đồ án tốt nghiệp Trong q trình làm việc với chị, em khơng ngừng tiếp thu thêm nhiều kiến thức bổ ích mà học tập tinh thần làm việc, thái độ nghiên cứu khoa học nghiêm túc, hiệu quả, điều cần thiết cho em trình học tập công tác sau Các anh chị thuộc Trung Tâm Quốc Gia Quan Trắc Cảnh Báo Mơi Trường Phòng Ngừa Dịch Bệnh Thủy Sản Khu Vực Nam Bộ- Viện Nghiên Cứu Nuôi Trồng Thủy Sản II Các bạn bè giúp đỡ em công việc tinh thần suốt thời gian làm đồ án Viện Nghiên Cứu Ni Trồng Thủy Sản II Do trình độ lý luận kinh nghiệm thực tiển hạn chế nên báo cáo khơng thể tránh khỏi thiếu sót, em mong nhận ý kiến đóng góp thầy, cô bạn để em học thêm nhiều kinh nghiệm hoàn thành tốt ii ĐỒ ÁN TỐT NGHIỆP MỤC LỤC LỜI CAM ĐOAN - i LỜI CẢM ƠN - ii MỤC LỤC - iii DANH MỤC CÁC BẢNG - vi DANH MỤC CÁC HÌNH - vii LỜI MỞ ĐẦU - 1 Đặt vấn đề Mục tiêu nghiên cứu: Nội dung nghiên cứu: CHƯƠNG 1.TỔNG QUAN TÀI LIỆU - 1.1 Giới thiệu cá tra Phân loại 1.1.2 Phân bố 1.1.3.Đặc điểm hình thái 1.1.4.Tập tính sinh trưởng 1.1.5.Đặc điểm sinh sản 1.1.6.Tình hình sản xuất tiêu thụ cá tra 1.2 Vi khuẩn Aeromonas hydrophila 1.2.1.Tình hình nghiên cứu A hydrophila 1.2.3.Đặc điểm phát triển 1.2.4 Sự phân bố 10 1.2.5.Dấu hiệu bệnh lý 11 1.2.6.Các nhân tố gây độc A hydrophila 13 1.2.7.Cấu trúc gen A hydrophila 13 1.2 8.Các gen gây độc A hydrophila 14 1.3.Phương pháp PCR ( Polymerase Chain Reaction) 16 1.3.1.Nguyên lý chung phương pháp PCR 16 1.3.2.Kỹ thuật giải trình tự DNA 18 1.4.Các nghiên cứu phát sinh loài 20 1.4.1.Nghiên cứu dựa trình tự gen 16S rRNA 21 iii ĐỒ ÁN TỐT NGHIỆP 1.4.2.Nghiên cứu dựa trình tự “housekeeping” gen 22 1.4.3 Nghiên cứu dựa kỹ thuật protein profile 23 CHƯƠNG VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU -24 2.1 Thời gian địa điểm làm thí nghiệm: 24 2.2.Vật liệu -24 2.3.1 Thiết bị, dụng cụ 25 2.3.2 Hóa chất 26 2.4 Phương pháp nghiên cứu -27 2.4.1 Nuôi cấy vi khuẩn 27 2.4.2 Phương pháp ly trích DNA 27 2.4.3 Quy trình PCR khảo sát gen độc 28 2.4.4 Quy trình PCR 16S rDNA 29 2.4.5 Quy trình PCR gen rpoD 30 2.4.6.Phương pháp điện di acid nucleotide gel agarose 30 2.4.7.Phương pháp xử lý kết giải trình tự 31 2.4.8.Phương pháp điện di SDS- PAGE 31 2.5 Bố trí thí nghiệm 33 2.5.1 Khảo sát diện gen gây độc lực A hydrophila 34 2.5.2 Giải trình tự đoạn gen 16S rDNA rpoD 34 2.5.3 Xác định đa dạng protein tổng số A hydrophila 34 2.5.4 Thiết lập mối tương đồng di truyền chủng 34 CHƯƠNG KẾT QUẢ VÀ THẢO LUẬN 35 3.1.Kết khảo sát gen độc lực 35 3.2 Kết giải trình tự đoạn gen 16S rDNA rpoD -38 3.2.1.Kết PCR khuếch đại đoạn 16S rDNA rpoD 38 3.2.2 Kết phân tích trình tự 39 3.4 Mối tương đồng di truyền chủng vi khuẩn khảo sát 45 3.4.1 Mối quan hệ kiểu gen độc lực 45 3.4.2 Mối quan hệ kiểu gen kết giải trình tự 46 3.4.3 Mối quan hệ kiểu gen protein tổng số 46 CHƯƠNG KẾT LUẬN VÀ ĐÊ NGHỊ 47 TÀI LIỆU THAM KHẢO -48 PHỤ LỤC -55 iv ĐỒ ÁN TỐT NGHIỆP DANH MỤC CÁC CHỮ VIẾT TẮT APS Ammonium Persulfate BA Blood Agar (thạch máu) BHIB Brain Heart Infusion Borth CFU Colony Forming Unit (đơn vị tạo thành khuẩn lạc) Ctv Cộng tác viên D_FISH Tổng cục Thủy sản–Bộ Nông Nghiệp Phát Triển Nông Thôn ĐBSCL Đồng Bằng Sông Cửu Long DNA Deoxyribonucleic acid EDTA Ethylene Diamine Tetra acetic acid KDa Kilo Dalton LD Lethal dose (Liều gây chết) PCR Polymerase Chain Reaction rDNA Ribosome Deoxyribonucleic acid rRNA Ribosomal ribonucleic acid SDS Sodium dodecyl sulfate SDS- PAGE Sodium dodecyl sulfate- polyacrylamide gel electrophoresis TBE Tris/ Borat/ EDTA ( Ethylenediaminetetraacetic acid) TEMED N, N, N’,N’- tetramethylenediamide UV Ultra violet VASEP Hiệp Hội chế biến xuất thủy sản Việt Nam (Vietnam Association of Seafood Exporters and Producers) w/v Weight for volume v ĐỒ ÁN TỐT NGHIỆP DANH MỤC CÁC BẢNG Bảng Thông tin nguồn gốc độc lực chủng vi khuẩn cá tra………24 Bảng 2 Các trình tự mồi xác định loài gen độc A hydrophila………….26 Bảng Quy trình luân nhiệt khuếch đại gen 16S rRNA……………………….… 30 Bảng Quy trình luân nhiệt khuếch đại gen rpoD……………………………… 30 Bảng Nhóm di truyền mẫu phân lập A hydrophila……………………….37 Bảng Độ tương đồng di truyền (%) trình tự 16S rDNA rpoD…… .40 vi ĐỒ ÁN TỐT NGHIỆP DANH MỤC CÁC HÌNH Hình 1 Cá tra Pangasianodon hypophthalmus …………………………………… Hình Aeromonas hydrophila…………………………………………………………9 Hình Biểu bệnh lý bên bên ngồi cá tra nhiễm A hydrophila 12 Hình Kích thước gen A hydrophila…………………………………………………….13 Hình Sơ đồ kỹ thuật PCR chuẩn ……………………………………………… 17 Hình Kỹ thuật giải trình tự………………………………………………… … 19 Hình 2.1 Sơ đồ bố trí thí nghiệm…………………………………………………………33 Hình Kết PCR khảo sát gen độc vi khuẩn A hydrophila………….….36 Hình Vi khuẩn A hydrophila mơi trường BA ………………… …….…….39 Hình 3.Kết PCR khuếch đại đoạn 16S rDNA……………….……………….…….39 Hình Kết PCR khuếch đại đoạn gen rpoD………………………………… …39 Hình Cây phát sinh lồi dựa trình tự 16S rRNA ……… …………………… 39 Hình Cây phát sinh lồi dựa trình tự rpoD……………………… … … … 41 Hình 3.7 Kết điện di SDS-PAGE…………………………………… ………… 43 Hình Mối quan hệ kiểu gen độc lực vi khuẩn A hydrophila…….….…45 vii ĐỒ ÁN TỐT NGHIỆP LỜI MỞ ĐẦU Đặt vấn đề Những năm gần đây, nghề nuôi trồng thủy sản không ngừng phát triển ngày có vị trí quan trọng kinh tế Việt Nam Đồng Bằng Sông Cửu Long (ĐBSCL) vùng đất giàu tiềm thiên nhiên ưu đãi có lợi lớn để phát triển nuôi trồng thủy sản Nuôi trồng thủy sản ĐBSCL có bước phát triển vượt bậc mang lại hiệu kinh tế cao, đặc biệt cá tra đối tượng nuôi chiến lược, thu lại nguồn ngoại tệ lớn, xem nguồn xuất thủy sản quan trọng nước ta Tuy nhiên với gia tăng mức phong trào nuôi cá tra ĐBSCL, tạo điều kiện cho dịch bệnh thường xuyên bộc phát gây nhiều thiệt hại cho người nuôi Trong đó, vi khuẩn coi tác nhân gây bệnh nghiêm trọng gây tỉ lệ hao hụt cao cá tra Đặc biệt vi khuẩn Aeromonas hydrophila gây bệnh xuất huyết tác nhân gây bệnh làm tổn thất lớn cho người nuôi năm Vi khuẩn A hydrophila xem đa dạng di truyền nghiên cứu nước di truyền vi khuẩn hạn chế Sự phân loại chi Aeromonas thường phức tạp, khó khăn việc nhận biết kiểu hình Aeromonas sử dụng phương pháp khác nhau, điều kiện test sinh hóa, biến đổi kiểu hình loài Ngày nay, với phát triển hiểu biết sinh học phân tử, nhiều thị sinh học phân tử nghiên cứu ứng dụng công cụ hỗ trợ đắc lực cho công tác nghiên cứu khả gây bệnh chủng A hydrophila Để góp phần xác định đa dạng di truyền chủng gây bệnh A hydrophila, tìm liên hệ kiểu gen độc lực vi khuẩn nhằm có đươc biện pháp hiệu hạn chế thiệt hại bệnh gây ra, đề tài “Sự khác biệt di truyền chủng Aeromonas hydrophila phân lập cá tra (Pangasianodon hypophthalmus) Đồng Bằng sông Cửu Long” thực Mục tiêu nghiên cứu: Đánh giá khác biệt di truyền chủng A hydrophila phân lập cá tra ĐBSCL phương pháp sinh học phân tử ĐỒ ÁN TỐT NGHIỆP Nội dung nghiên cứu: Khảo sát diện gen gây độc A hydrophila phương pháp PCR xác định đa dạng protein tổng số vi khuẩn 18 chủng A hydrophila phân lập cá tra Giải trình tự đoạn gen 16S rRNA rpoD 18 chủng vi khuẩn để khẳng định lại định danh lồi kiểm tra sinh hóa, đồng thời thiết lập phát sinh lồi tìm mối tương đồng di truyền với kiểu gen ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg agctggtctgagaggatgatcagccacactggaactgagacacggtccagactcctacgg ************************************************************ 296 294 292 294 294 294 300 299 294 300 294 294 296 296 294 296 295 294 292 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt gaggcagcagtggggaatattgcacaatgggggaaaccctgatgcagccatgccgcgtgt ************************************************************ 356 354 352 354 354 354 360 359 354 360 354 354 356 356 354 356 355 354 352 59 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggcgcctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttgttggctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggtcagtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggtagctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggcgcctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggcgcctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttggcgcctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttgatgcctaat gtgaagaaggccttcgggttgtaaagcactttcagcgaggaggaaaggttgatgcctaat ************************************************* ***** 416 414 412 414 414 414 420 419 414 420 414 414 416 416 414 416 415 414 412 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 acgtgtcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acccgctggctgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg atctgctgactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg aactgccagctgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acgtgtcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acgtgtcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acgtgtcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acgtatcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg acgtatcaactgtgacgttactcgcagaagaagcaccggctaactccgtgccagcagccg * *************************************************** 476 474 472 474 474 474 480 479 474 480 474 474 476 476 474 476 475 474 472 60 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg cggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcg ************************************************************ 536 534 532 534 534 534 540 539 534 540 534 534 536 536 534 536 535 534 532 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc gttggataagttagatgtgaaagccccgggctcaacctgggaattgcatttaaaactgtc ************************************************************ 596 594 592 594 594 594 600 599 594 600 594 594 596 596 594 596 595 594 592 61 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat cagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagat ************************************************************ 656 654 652 654 654 654 660 659 654 660 654 654 656 656 654 656 655 654 652 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaa ************************************************************ 716 714 712 714 714 714 720 719 714 720 714 714 716 716 714 716 715 714 712 62 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt agcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgatt ************************************************************ 776 774 772 774 774 774 780 779 774 780 774 774 776 776 774 776 775 774 772 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg tggaggctgtgtccttgagacgtggcttccggagctaacgcgttaaatcgaccgcctggg ************************************************************ 836 834 832 834 834 834 840 839 834 840 834 834 836 836 834 836 835 834 832 63 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag gagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggag ************************************************************ 896 894 892 894 894 894 900 899 894 900 894 894 896 896 894 896 895 894 892 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc catgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatgtctggaatc ************************************************************ 956 954 952 954 954 954 960 959 954 960 954 954 956 956 954 956 955 954 952 64 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgtagagatacgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca ctgcagagatgcgggagtgccttcgggaatcagaacacaggtgctgcatggctgtcgtca *** ****** ************************************************* 1016 1014 1012 1014 1014 1014 1020 1019 1014 1020 1014 1014 1016 1016 1014 1016 1015 1014 1012 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctgtcctttgttg ************************************************************ 1076 1074 1072 1074 1074 1074 1080 1079 1074 1080 1074 1074 1076 1076 1074 1076 1075 1074 1072 65 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ccagcacgtaatggtgggaactcaagggagactgccggtgataaaccggaggaaggtggg ************************************************************ 1136 1134 1132 1134 1134 1134 1140 1139 1134 1140 1134 1134 1136 1136 1134 1136 1135 1134 1132 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg gatgacgtcaagtcatcatggcccttacggccagggctacacacgtgctacaatggcgcg ************************************************************ 1196 1194 1192 1194 1194 1194 1200 1199 1194 1200 1194 1194 1196 1196 1194 1196 1195 1194 1192 66 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacaaagggctgcaagctagcgatagtgagcgaatcccagaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga tacagagggctgcaagctagcgatagtgagcgaatcccaaaaagcgcgtcgtagtccgga **** ********************************** ******************** 1256 1254 1252 1254 1254 1254 1260 1259 1254 1260 1254 1254 1256 1256 1254 1256 1255 1254 1252 B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 tcggagtttgcaactcgatccggaatt gaatcgctagtaatcgcaaatcag-aatt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt ttggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtctgcaactcgactccgtgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtctgcaactcgactccgtgaagtcggaatcgctagtaatcgcgaatcagaatgt tcggagtctgcaactcgactccgtgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtttgcaactcgactccgtgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtctgcaactcgactccgtgaagtcggaatcgctagtaatcgcaaatcagaatgt tcggagtctgcaactcgactccgtgaagtcggaatcgctagtaatcgcaaatcagaatgt * ***** ********** * ***************** ****** * * 1311 1314 1312 1314 1314 1314 1320 1319 1314 1320 1314 1314 1316 1316 1314 1316 1315 1314 1312 67 ĐỒ ÁN TỐT NGHIỆP B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatg-ggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt cgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacaaccatgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt tgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacca-tgggagtgggtt ******************************************* * ********** B66 B168 B110 B12 B88 B5 B77 B38 N48 B86 B99 B85 B10 B141 N35 K28 B56 P11 ATCC7966 gcaccagaagtagatagcttatagccttccgggagggcgtta gcaccagaagtagatagcttaaccttcgggaggg -gcaccagaagtagatagcttaaccttcgggagggcgttac-gcaccagaagtagatagcttaaccttcgggagggcgtttacc gcaccagaagtagatagcttaacc -gcaccagaagtagatagctt -gcaccagaagtagatagcttaaccttcgggaggg -gcaccagaagtagatagct gcaccagaagtagatagcttaacc -gcaccagaagtagatagcttaaccttcgggagggcgttac-gcaccagaagtagatagc -gcaccagaagtagatagcttaaccttcgggagggcgttac-gcaccagaagtagatagcttaaccttcgggagggcgttac-gcaccagaagtagatagcttaaccttccgggaggg gcaccagaagtagatagctt -gcaccagaagtagatagctta gcaccagaagtagatagctt -gcaccagaagtagatagcttaaccttcgggagggcg -gcaccagaagtagatagcttaaccttcgggagggcg -****************** 68 1412 1407 1411 1415 1397 1393 1413 1397 1397 1419 1391 1413 1415 1410 1393 1397 1394 1409 1407 1370 1373 1371 1373 1373 1373 1379 1378 1373 1379 1373 1373 1375 1375 1373 1376 1374 1373 1371 ĐỒ ÁN TỐT NGHIỆP B.2 Trình tự nucleotide rpoD N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 cagttcctggatttgatccaggaaggcaacataggtttgatgaaagcggtagac tcctggatttgatccaggagggcaacatcggtctgatgaaggcggtcgac tcctggatttgatccaggaaggcaacatcggcttgatgaaggcggtcgac -cctggatttgatccaggaaggcaacatcggtttgatgaaggcggtcgac cagttcctggatctgatccaggaaggcaacatcggtctgatgaaggcggtcgac ggtttgcagttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtagac -tggatttgatccaggaaggcaacatcggtttgatgaaggcggtcgac -cctggatttgatccaggaaggcaacatcggtttgatgaaggcggtcgac cagttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtcgac -ttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtcgac tgcagttcctggatttgatccaggaaggcaacatcggtttgatgaaggcggtcgac -ttcctggatttgatccaggaaggcaacatcggtttgatgaaggcggtcgac tcctggatttgatccaggagggcaacatcggtttgatgaaggcggtagac -ttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtagac ctggatttgatccaggagggcaacatcggtctgatgaaggcggtagac tgcagttcctggatttgatccaggagggcaacatcggtctgatgaaggcggtagac -ttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtagac tgcagttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtagac -gtctgcagttcctggatttgatccaggaaggcaacatcggtctgatgaaggcggtagac ***** ********** ******** ** ******* ***** *** 54 50 50 49 54 60 47 49 54 51 56 51 50 51 48 56 51 56 59 N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 aagtttgaataccgtcgcggctacaagttctcgacctatgccacctggtggatccgtcag aagttcgaatatcgtcgcggctacaagttctcgacctatgccacctggtggatccgtcag aagtttgaatattgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaatatcgtcgtggctacaagttctcgacctatgcaacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttttcgacctatgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgcaacctggtggatccgtcag aagtttgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttctcgacctacgccacctggtggatccgtcag aagtttgaataccgtcgtggctacaagttctcgacctatgcaacctggtggatccgtcag aagtttgaataccgtcgtggctacaagttctcgacctatgctacctggtggatccgtcag aagtttgaataccgtcgtggctacaagttctcgacctatgcgacctggtggatccgtcag aagtttgaataccgtcgtggctacaagttctcgacctatgcgacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttttcgacctatgcgacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttttcgacctatgcgacctggtggatccgtcag aagttcgaataccgtcgtggctacaagttttcgacctatgcgacctggtggatccgtcag ***** ***** **** *********** ******** ** ****************** 114 110 110 109 114 120 107 109 114 111 116 111 110 111 108 116 111 116 119 69 ĐỒ ÁN TỐT NGHIỆP N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 gccattacccgctccatcgcggatcaggcccgtactatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtactatccgtatcccggtgcacatgatc gccatcacccgctccatcgcggatcaggcccgtaccatacgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtgcacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgaatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacatatgatc gccattacccgctccatcgcggatcaggcccgaaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgaaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc gccattacccgctccatcgcggatcaggcccgtaccatccgtatcccggtacacatgatc ***** ************************** ** ** ** ******** ** ****** 174 170 170 169 174 180 167 169 174 171 176 171 170 171 168 176 171 176 179 N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgcatctcccgccagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagactatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccattaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatggggcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatggggcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatggggcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatggggcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgcgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa gagaccatcaacaagctcaaccgtatctcccgtcagatgctgcaggagatgggtcgtgaa ***** ** ************** ******** ******************** ** *** 234 230 230 229 234 240 227 229 234 231 236 231 230 231 228 236 231 236 239 70 ĐỒ ÁN TỐT NGHIỆP N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 ccgaatccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgtatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggtatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgtaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgtaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ccgaacccggaagagctggccatgcgcatgggcatgccggaagacaagatccgcaaggta ***** ******************** ***** ******************** ****** 294 290 290 289 294 300 287 289 294 291 296 291 290 291 288 296 291 296 299 N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 ctgaaaattgccaaagagcctatctccatggagacgcccattggtgatgatgaagattcc ctgaaaatcgccaaggagcccatctccatggagacgcccatcggtgatgacgaagactcc ttgaagatcgccaaagagcccatctccatggagacgcccatcggcgatgatgaagactcc ttgaagatcgccaaagagcctatctccatggagacgcctatcggtgatgatgaagactcc ctgaaaatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagactcc ctgaaaatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagactcc ttgaaaatcgctaaagagcctatctccatggagacgcccatcggtgatgatgaagactcc ttgaagatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagattcc ttgaaaatcgccaaagagcccatctccatggagacgcctatcggtgatgatgaagactcc ttgaaaatcgccaaagagcccatctccatggagacgcctatcggtgatgatgaagactcc ttgaaaatcgccaaagagcccatctccatggagacgcctatcggtgatgatgaagactcc ttgaaaatcgccaaagagcccatctccatggagacgcctatcggtgatgatgaagactcc ctgaagatcgccaaagagcctatctccatggagacgcccatcggtgatgatgaagattcc ctgaaaatcgccaaagaacctatctccatggagacgcccatcggtgatgatgaagattcc ttgaagatcgccaaagagcccatatccatggagacgcccatcggtgatgatgaagattcc ttgaagatcgccaaagagcccatatccatggagacgcccatcggtgatgatgaagattcc ttgaagatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagattcc ttgaagatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagattcc ttgaagatcgccaaagagcccatctccatggagacgcccatcggtgatgatgaagattcc **** ** ** ** ** ** ** ************** ** ** ***** ***** *** 354 350 350 349 354 360 347 349 354 351 356 351 350 351 348 356 351 356 359 71 ĐỒ ÁN TỐT NGHIỆP N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 cacttgggcgacttcatcgaagataatacgcttgctttgccagctgacgctgcgaccagc cacttgggcgacttcatcgaagataccaccctggctctgccagcggatgcggccaccagc cacttgggcgatttcatcgaagataccactttggccctgccggccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccggccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgcctgcagatgcggccaccagt cacttgggcgacttcatcgaagataccaccctggccctgccggccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccggccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggctctgccggccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccagccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccagccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccagccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggccctgccagccgatgcggccaccagc cacttgggcgacttcatcgaagataccaccctggctctgccagcggatgcggcgaccagc cacttgggcgactttatcgaagataccaccctggctctgccagcggatgcggcgaccagc cacttgggtgacttcatcgaagataccaccctggctctgccagcggatgctgccactagc cacttgggtgacttcatcgaagataccaccctggctctgccagcggatgctgccactagc cacttgggcgacttcatcgaagataccaccctggctttgccagcggatgctgctaccagc cacttgggcgacttcatcgaagataccaccctggctttgccagcggatgctgctaccagc cacttgggcgacttcatcgaagataccaccctggctttgccagcggatgctgctaccagc ******** ** ** ********** ** * ** **** ** ** ** ** ** ** 414 410 410 409 414 420 407 409 414 411 416 411 410 411 408 416 411 416 419 N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 gagagtctgcgcaacgctacccgcgaagtactggccggtctgaccgcacgcgaagccaag gagagcctgcgcaacgcgacccgcgaagtgctggccgggctgaccgcacgggaagccaag gagagcctgcgcaacgcgacccgcgaagtactggccggcctgaccgcccgcgaagccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgcccgcgaggccaag gagagcctgcgcaacgccacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gagagcctgcgcaacgcgacccgcgaagtgctggccggtctgaccgctcgtgaggccaag gaaagcctgcgcaacgccacccgcgaagtgcttgctggcctgaccgcccgcgaagccaag gaaagcctgcgcaacgccacccgcgaagtgctggctggcctgaccgcccgcgaagccaag gagagcctgcgcaacgccacccgcgaagtgctggccggcctgaccgcccgcgaggccaag gagagcctgcgcaacgccacccgcgaagtgctggccggcctgaccgcccgcgaggccaag gagagcctgcgcaacgccacccgcgaagtgctggccggcttgaccgcccgtgaggctaag gagagcctgcgcaacgccacccgcgaagtgctggccggcttgaccgcccgtgaggctaag gagagcctgcgcaacgccacccgcgaagtgctggccggcttgaccgcccgtgaggctaag ** ** *********** *********** ** ** ** ******* ** ** ** *** 474 470 470 469 474 480 467 469 474 471 476 471 470 471 468 476 471 476 479 72 ĐỒ ÁN TỐT NGHIỆP N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 gtgctgcggatgcgtttcgggatcgacatgaacactgaccacacccttgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacaccgaccacaccctcgaggaagtgggc gtgctgcgcatgcgtttcggtatcgacatgaacaccgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacacccttgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacacccttgaggaagtgggc gtattgcgcatgcgtttcggtatcgacatgaacactgaccacacccttgaggaagtgggc gtattgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcggatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcggatgcgtttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc gtactgcgcatgcgcttcggtatcgacatgaacactgaccacaccctcgaggaagtgggc ** **** ***** ***** ************** *********** ************ N35 B168 B110 N48 B88 B86 B99 B85 B10 B38 B12 B77 B141 B5 P11 ATCC7966 K28 B56 B66 aaacagttcgacgttacccg aagcagttcgacgtta aagcagttcgacgttacccg aagcagttcgacgttaccg -aagcagttcgacgttacc aagcagttcgacgttacccg aagcagttcgacgttacccg aagcagttcgacgttacccg aagcagttcgacgt aagcagttcgacgttacccg aagcagttcgacgttacccgtgagc aagcagttcgacgttaccc -aaacagttcgac aagcagttcgacgttacccg aagcagttcgacgtt -aagcagttcgacgttacccg aagcagttcgacgttacccg aagcagttcgacgttacc aagcagttcgacgttacccg ** ********* 554 546 550 548 552 560 547 549 548 551 561 550 542 551 543 556 551 554 559 73 534 530 530 529 534 540 527 529 534 531 536 531 530 531 528 536 531 536 539 ... tra (Pangasianodon hypophthalmus) Đồng Bằng sông Cửu Long thực Mục tiêu nghiên cứu: Đánh giá khác biệt di truyền chủng A hydrophila phân lập cá tra ĐBSCL phương pháp sinh học phân tử ĐỒ ÁN TỐT... bệnh A hydrophila, tìm liên hệ kiểu gen độc lực vi khuẩn nhằm có đươc biện pháp hiệu hạn chế thiệt hại bệnh gây ra, đề tài Sự khác biệt di truyền chủng Aeromonas hydrophila phân lập cá tra (Pangasianodon. .. rpoD……………………………… 30 Bảng Nhóm di truyền mẫu phân lập A hydrophila …………………….37 Bảng Độ tương đồng di truyền (%) trình tự 16S rDNA rpoD…… .40 vi ĐỒ ÁN TỐT NGHIỆP DANH MỤC CÁC HÌNH Hình 1 Cá tra Pangasianodon