Nghiên cứu sự biến đổi thành phần gen nhân ITS 2, 28s rRNA và gen ty thể COX1 của loài sán lá gan lớn fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại việt nam

i ĐẠI HỌC THÁI NGUYÊN TRƯỜNG ĐẠI HỌC KHOA HỌC - NGUYỄN THỊ HOA ÁNH NGHIÊN CỨU BIẾN ĐỔI THÀNH PHẦN GEN NHÂN ITS-2, 28S rRNA VÀ GEN TY THỂ COX1 CỦA LOÀI SÁN LÁ GAN LỚN FASCIOLA SSP DẠNG THUẦN VÀ DẠNG LAI GÂY BỆNH TRÊN ĐỘNG VẬT TẠI VIỆT NAM TĨM TẮT LUẬN VĂN THẠC SĨ CƠNG NGHỆ SINH HỌC Chuyên ngành: Công nghệ sinh học Mã số: 60 42 02 01 Người hướng dẫn khoa học: TS NGUYỄN THỊ BÍCH NGA THÁI NGUYÊN - 2016 i LỜI CAM ĐOAN Tơi xin cam đoan: Đây cơng trình nghiên cứu khoa học trực tiếp thực số kết cộng tác với cộng khác Các số liệu trình bày luận văn hồn tồn trung thực xác Ngày…… tháng … năm 201… Học viên Nguyễn Thị Hoa Ánh ii LỜI CẢM ƠN Tơi xin chân thành bày tỏ lòng biết ơn sâu sắc đến: - TS Nguyễn Thị Bích Nga người trực tiếp hướng dẫn, tạo điều kiện thuận lợi cho suốt trình nghiên cứu, học tập hồn thành luận văn - Cảm ơn cán Phòng Miễn dịch học – Viện Công nghệ sinh học nơi thực tập nhiệt tình giúp đỡ tơi hồn thành luận văn Tơi xin gửi lời cảm ơn đến: - Ban Giám đốc Đại học Thái Nguyên, Ban Giám hiệu Trường Đại học Khoa học, Phòng Đào tạo Quan hệ Quốc tế, Khoa Khoa học sống - Trường Đại học Khoa học - Đại học Thái Nguyên - Sự tài trợ kinh phí Quỹ phát triển Khoa học công nghệ quốc gia (Nafosted) cho đề tài mã số 106-YS.02-2013.06 TS Nguyễn Thị Bích Nga làm chủ nhiệm - Cuối muốn bày tỏ lòng biết ơn sâu sắc đến người thân ln khuyến khích,động viên chia sẻ khó khăn với tơi suốt q trình học tập hồn thành nghiên cứu Học viên Nguyễn Thị Hoa Ánh iii MỤC LỤC LỜI CAM ĐOAN i LỜI CẢM ƠN ii MỤC LỤC iii DANH MỤC CÁC CHỮ VIẾT TẮT vi v DANH DANH MỤC MỤC vii BẢNG BIỂU HÌNH ẢNH MỞ ĐẦU CHƯƠNG 1:TỔNG QUAN TÀI LIỆU 1.1 Giới thiệu loài sán gan lớn 1.1.1.Vị trí phân loại sán gan lớn hệ thống phân loại động vật 1.1.2.Đặc điểm hình thái học sán gan lớn Fasciola sp 1.1.3.Chu kỳ sinh trưởng phát triển sán 1.1.4.Trứng sán gan lớn 1.1.5.Sán trưởng thành 1.2.Đặc điểm bệnh sán gan lớn 10 1.2.1.Cơ chế sinh bệnh 10 1.2.2 Đặc điểm dịch tễ bệnh sán Fasciola trâu, bò Việt Nam 12 1.3 Tầm quan trọng nghiên cứu giám định lồi phương pháp sinh học phân tử giải trình tự 17 1.3.1 Giới thiệu sơ hệ gen nhân ribosomal DNA (rDNA) 18 1.3.2 Hệ gen DNA ty thể 19 CHƯƠNG 2:VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 22 2.1 Vật liệu nghiên cứu 22 2.2 Thời gian địa điểm nghiên cứu 22 2.3 Dụng cụ thiết bị hóa chất 22 2.3.1 Dụng cụ, trang thiết bị 22 2.3.2 Hóa chất 22 2.4 Phương pháp nghiên cứu 23 iv 2.4.1 Phương pháp thu thập bảo quản mẫu Fasciola sp 24 2.4.2 Phương pháp tách chiết DNA tổng số 24 2.4.3 Thiết kế mồi, thực PCR gen ITS2, 28S gen cox1 25 2.4.4 Phương pháp giải trình tự 27 2.4.5 Phương pháp phân tích xử lý số liệu 28 CHƯƠNG 3:KẾT QUẢ NGHIÊN CỨU VÀ BÀN LUẬN 30 3.1 Thu nhận gen ITS-2 chẩn đoán phân biệt 30 3.1.1 Thu thập mẫu sán gan lớn phân loại hình thái 30 3.1.2 Sử dụng gen ITS-2 chẩn đoán phân biệt 30 3.2 Kết thu nhận gen 28s xây dựng phả hệ 35 3.3 Kết thu nhận chuỗi gen ty thể COX1 39 3.3.1 Kết giải trình tự gen cox1 Fasciola sp Việt Nam so sánh với loài thuộc lớp sán Trematoda 41 3.3.2 Xây dựng mối quan hệ phả hệ loài thuộc lớp sán 47 KẾT LUẬN 53 TÀI LIỆU THAM KHẢO 54 v DANH MỤC CÁC CHỮ VIẾT TẮT DNA Deoxyribonucleotde AST Abnormal Spermatogenic Type NST Normal Spermatogenic Type Cox1 cytochrome oxidase subunit ITS-2 Second internal transcribed spacer Rdna Ribosome DNA SLGL Sán gan lớn PCR Polymerase chain reacton ddNTP dideoxyrinonucleotide triphosphate dNTP bp deoxyrinonucleotide triphosphate Cặp TAE bazơ (base pair) PAGE Tris base-Acetic-EDTA Điện EtBr di gel polyacylamid Ethidium EDTA Bromide OD Ethylene Diamine Tetraacemic Acid Kb Mật độ quang học Kda Kilobase Kilodalton µl Microlitre µm Micrometer mm Millimeter DANH MỤC BẢNG BIỂU Bang 2.1 Danh sách cac mồi dung phan ưng PCR thu nhận gen ITS-2, 28S COX1 25 Bảng 3.1 So sánh nucleotide vị trí biến đổi vùng giao gen ITS-2 mẫu sán gan lớn vùng địa lý vật chủ khác 33 Bảng 3.2 Các mẫu cung cấp chuỗi gen 28S nghiên cứu 36 Bảng 3.3 Các mẫu sán Trematoda Ngân hàng gen cung cấp chuỗi cox1 sử dụng so sánh đối chiếu nghiên cứu 40 Bảng 3.4 Tỷ lệ tương đồng trình tự amino acid gen cox1 loài sán thuộc lớp Trematoda 46 vii DANH MỤC HÌNH ẢNH Hình 1.1 Hình ảnh mơ tả hình thái sán gan lớn Fasciola sp Hình 1.2 Chu kỳ sinh trưởng phát triển SLGL Fasciola sp ký sinh vật chủ động vật người Hình 1.3 Hình ảnh trứng sán gan lớn Fasciola sp phóng đại 400 lần kính hiển vi điện tử Hình 1.4 Hình thể sán gan lớn Fasciola sp trưởng thành Hình 1.5 Mơ hình cấu trúc ribosome DNA gen nhân vùng gen cần nghiên cứu18 Hình 1.6 Mơ hình cấu trúc DNA hệ gen ty thể 20 Hình 2.1 Sơ đồ quy trình nghiên cứu giải mã hệ gen ty thể Fasciola sp 23 Hình 3.1 Hình ảnh sán gan lớn FspNB-VN FspCB-VN nghiên cứu 30 Hình 3.2 Điện di kiểm tra sản phẩm PCR vùng gen ITS-2 31 Hình 3.3 So sánh trình tự chuỗi nucleotide ITS-2của 16 mẫu Fasciola sp 32 Hình 3.4 Điện di sản phẩm PCR gen 28S với cặp mồi U28SF – U28SR 35 Hình 3.5 Mối quan hệ nguồn gốc phả hệ mẫu Fasciola sp xác lập dựa thành phần nucleotide gen 28S ribosome chương trình MEGA6.06 38 Hình 3.6 Điện di kiểm tra sản phẩm PCR gen cox1 với cặp mồiFSP12F – FGHR2 40 Hình 3.7 Trình tự amino acid suy diễn FspNB-VN FspCB-VN so sánh với chuỗi gen cox1 thuộc lớp sán Trematoda 44 Hình 3.8 Cây phả hệ thể mối quan hệ loài mẫu sán dựa thành phần amino acid gen cox1 chương trình MEGA6 48 MỞ ĐẦU Lý chọn đề tài Bệnh sán gan lớn (fascioliasis) hai loài Fasciola hepatica Fasciola gigantica thuộc họ Fasciolidaekí sinh động vật nhai lại (trâu, bò, dê, cừu…) dần thích ứng gây bệnh người Trước đây, đặc tính hình thái học (morphology), sinh thái học (ecology) huyết học (serology) thường sử dụng chẩn đoán đáp ứng phần lớn yêu cầu phân loại sinh vật có ngành ký sinh trùng [5],[18] Tuy nhiên phân loại sán gan dựa vào hình thái khó phân định Đặc biệt có tượng lai dạng trung gian (hybrid form) quần thể sán gan phát hiện, hệ gen có trộn lẫn F hepatca F gigantca (Fh/Fg), hệ gen nhân tế bào mang gen từ dòng bố (F hepatca) hệ gen ty thể (F gigantca) mang tính di truyền theo dòng mẹ [7] Bệnh sán gan lớn coi bệnh ký sinh trùng bị lãng quên (neglected emerging infectious disease) tổ chức y tế giới (WHO) quan tâm Sán gan lớn gây bệnh người, ảnh hưởng đến sức khỏe cộng đồng, chúng gây tổn thương bệnh lý hệ thống gan mật, gây can xi hóa đường mật, ký sinh lạc chỗ di chuyển lạc lên não… gây tổn thương nặng nhu mô gan làm suy gan dẫn đến tử vong [4], [7], [17] Một số trường hợpdo bệnh sán gan lớn người gây phản ứng viêm, xơ hóa gan, dẫn đến chẩn đốn bị nhầm bệnh ung thư gan [2], [5] Đối với động vật ăn cỏ (trâu, bò, dê, cừu…) nhiễm sán gan lớn làm giảm trọng lượng vật, ốm yếu, thiếu máu bệnh lý, vật không sinh sản, gây thiệt hại lớn kinh tế [11] Vì việc phát sớm xác định xác lồi Fasciola gây bệnh cần thiết, có ý nghĩa thực tiễn chẩn đoán điều trị Chẩn đốn, giám định phân biệt lồi Fasciola thực phương pháp sinh học phân tử dựa thị gen nhân ty thể Xuất phát từ u cầu đó, chúng tơi tiến hành thực đề tài: “Nghiên cứu biến đổi thành phần gen nhân ITS-2, 28S rRNA gen ty thể COX1 loài sán gan lớn Fasciola sp dạng dạng lai gây bệnh động vật Việt Nam” Mục têu đề tài - Giải trình tự phân tích gen nhân ITS-2, gen 28S sán gan lớn dạng xác định dạng laigây bệnh động vậttại Việt Nam - Giải mã gen COX1 hệ gen ty thể F hepatca F gigantca dạng dạng lai, phân tích đặc điểm di truyền - Xây dựng phả hệ dựa trình tự gen ITS-2, 28S rRNA COX1 phân loại Nội dung nghiên cứu - Thu nhận sàng lọc mẫu Fasciola sp, giám định hình thái học nhận dạng dòng lai dựa vào vùng gen hệ gen nhân ITS-2 để phân biệt Fasciolagigantca Fasciola dạng lai (hybrid), so sánh phân tích liệu trình tự gen ITS-2 gen 28S, xây dựng phả hệ - Thu nhận gen ty thể COX1 gen ty thể, phân tích trình tự nucleotide/amino acid, xem xét mối quan hệ tiến hóa di truyền phả hệ - Phân tích biến đổi di truyền dịch tễ học phân tử: xác định khác biệt trình tự hệ gen nhân vùng ITS-2, 28S COX1 F hepatica F gigantica (thuần lai) Ý nghĩa khoa học thực tiễn - Góp phần cung cấp trình tự chuỗi gen ITS-2, 28S cox1 sán gan lớn Fasciola sp Việt Nam - Ngồi kết xác 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(2007), “Making the most of mitochondrial genomes-markers for phylogeny,molecular ecology and barcodes in Schistosoma (Platyhelminthes: Digenea)”, Int J Parasitol, 37(12), pp 140118 Tài liệu dịch 67 Skrjabin KI Petrov AM (1977), Nguyên lý môn giun tròn thú y, Bùi Lập Đồn Thị Băng Tâm dịch từ nguyên tiếng Nga, Nhà xuất khoa học kỹ thuật, Hà Nội, (1), tr 56 – 57 Tài liệu từ mạng internet 68 Hồ Việt Mỹ, Lê Quang Hùng, Trần Thị Kim Dung, Đặng Tất Thế (2000), http://www.dostbinhdinh.org.vn/kyYeu/KyyeuSTKT19972007/Giaidoan2002-2003/P101 - 102.htm 69 http://iranhelminthparasites.com/species/fasciola.htm 70 Sách “Ký sinh trùng côn trùng y học, Học viện Quân y, 2008 71 www.dpd.cdc.gov/dpdx Viện sốt rét kí sinh trùng côn trùng Trung ương 61 PHỤ LỤC Phụ lục 1.Trình tự nucleotde gen 28S * 20 * 40 * 60 * FspNB-VN : AATGAGGGTGAAAAGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCTGAGCAGA CCTTGGAGTCGG : 75 FspCB-VN : AATGAGGTTAAAAGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCTTGAGCAGAC CTTGGAGTCGGG : 75 FgigSN-AY2 : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCGGACC TTGGAGTCGGGT : 75 FgigTH-HM0 : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACC TTGGAGTCGGGT : 75 FgigKE-EU0 : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCGGACC TTGGAGTCGGGT : 75 FgET : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCTTGAGCGGAC CTTGGAGTCGGG : 75 FgEQB : AATGAGGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTCCCTGAGCAGAC CTTGGAGTCGGG : 75 FhEQB : AATGAGGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCCTGAGCAG ACCTTGGAGTCG : 75 FspEQO : AATGAAGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGGATGGCCCAGTATCTTCCTGAGCAGA CCTTGGAGTCGG : 75 FhAus : AATGAAGGGGAAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCTGAGCA GACCTTGGAGTCG : 75 FhBe : AATGAGGGTGAAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCCTGAGCA GACCTTGGAGTC : 75 FhepEG-EU0 : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACC TTGGAGTCGGGT : 75 FhepSA-AY2 : AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACC TTGGAGTCGGGT : 75 FjacLK-EU0 : ATTGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACC TTGGAGTCGGGT : 75 FmagUS-EU0 : AGTGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACC TTGGAGTCGGGT : 75 FbusVN-EU0 : AGTGAGGGTGAAAGGCCCGTGGGGGTGGAGACTCAGATTGGACAGTATCTCCCTGAGCAGAC CTTGGAGTCGGGT : 75 80 * 100 * 120 * 140 * FspNB-VN TTGTTTGTGAATGCAGCCCGTTGTTTGTGAATGCAGCCCFspCB-VN 14 : AAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCG 914 FgigSNTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCCAY2 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FgigTHTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC14 HM0 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FgigKETGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC14 EU0 : : TTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAGGC AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FgET 15 TAAATACTAGCACGAGTCCGATAGCG : FgEQB TTGTTTGTGAATGCAGCCC14 : AAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCG 15 FhEQB GGTTGTTTGTGAATGCAGCCCCAAAGCGGGTGGTAAACTCCATCCCAAG : GCTAAATACTAGCACGAGTCCGATAG : 015 FspEQO GTTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAGG : CTAAATACTAGCACGAGTCCGATAGC : FhAus : GGTTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAG 15 GCTAAATACTAGCACGAGTCCGATAG : FhBe : GGGTTGTTTGTGAATGCAGCCCCAAAGCGGGTGGTAAACTCCATCCCAA 15 GGCTAAATACTAGCACGAGTCCGATA : 014 FhepEG- TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCCEU0 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FhepSATGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC14 AY2 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FjacLKTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC14 EU0 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FmagUSTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC14 EU0 : AAGGCTAAATACTAGCACGAGTCCGATAGCGA : FbusVNTGTTTGTGAATGCAGCCC-AAAGTGGGTGGTAAACTCCATCC14 EU0 : AAGGCTAAATACTTGCACGAGTCCGATAGCGA : 160 * 180 * 200 * 220 FspNB-VN AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTA GAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGT 22 FspCB-VN : AACAGTGCGTGAAACCGTTCAGAGGTAA : FgigSNACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 AY2 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : FgigTHACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 HM0 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : FgigKEACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 EU0 : : AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTA ACAGTGCGTGAAACCGTTCAGAGGTAAA : FgET 22 AACAGTGCGTGAAACCGTTCAGAGGTAA : FgEQB AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTA 22 : AACAGTGCGTGAAACCGTTCAGAGGTAA : 422 FhEQB CGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAG : TAAACAGTGCGTGAAACCGTTCAGAGGT : FspEQO GAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGT 22 : AAACAGTGCGTGAAACCGTTCAGAGGTA : FhAus : CGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAG 22 TAAACAGTGCGTGAAACCGTTCAGAGGT : FhBe : GCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGA 22 GTAAACAGTGCGTGAAACCGTTCAGAGG : FhepEG- ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 EU0 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : FhepSAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 AY2 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : FjacLKACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 EU0 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : FmagUSACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA 22 EU0 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : 322 FbusVNACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAA EU0 : ACAGTGCGTGAAACCGTTCAGAGGTAAA : * 240 * 260 * 280 * 300 FspNB-VN FspCB-VN AACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTAT ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATG 29 : GCATGAGCTTGGTCATATTGGTTGTGT : FgigSNCAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGG 29 AY2 : CATGAGCTTGGTCATATTGGTTGTGTG : FgigTHCAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGG 29 HM0 : CATGAGCTTGGTCATATTGGTTGTGTG : FgigKECAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGG 29 EU0 : : ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATG CATGAGCTTGGTCATATTGGTTGTGTG : FgET 30 GCATGAGCTTGGTCATATTGGTTGTGT : 029 FgEQB ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATG : GCATGAGCTTGGTCATATTGGTTGTGT : FhEQB AAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTA 30 : TGGCATGAGCTTGGTCATATTGGTTGT : FspEQO AACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTAT 30 : GGCATGAGCTTGGTCATATTGGTTGTG : FhAus : AAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTA 30 : FhBe : TGGCATGAGCTTGGTCATATTGGTTGT TAAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGT 30 ATGGCATGAGCTTGGTCATATTGGTTG : FhepEG- CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGG 29 EU0 : CATGAGCTTGGTCATATTGGTTGTGTG : FhepSACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGG 29 AY2 : CATGAGCTTGGTCATATTGGTTGTGTG : FjacLKCAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTGTGG 29 EU0 : CATGAGCTTGGTCATGTGGGTTGTGTG : 829 FmagUSCAGGTGGAGTTGAACTGCAAGCTCCGAGGATTCAGCTGGTGAGTATGG EU0 : CATGATCTTGGTCATATGGGTTGTGTG : FbusVNCAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGATTGG 29 EU0 : CATGATCTTGGTCATGTGGGTTGTGTT : * 320 * 340 * 360 * FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB : : : : : : : TGCTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCG GCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG GCTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG GCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG : : : : : : : 374 374 373 373 373 375 374 62 FhEQB GTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCG FspEQO TGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGA : ATCACTCATCAGGTGTTGTGCGCCCG : FhAus : GTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCG AATCACTCATCAGGTGTTGTGCGCCC : FhBe : TGTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGC GAATCACTCATCAGGTGTTGTGCGCC : FhepEG- CTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAAT EU0 : CACTCATCAGGTGTTGTGCGCCCGGG : FhepSACTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAAT AY2 : CACTCATCAGGTGTTGTGCGCCCGGG : FjacLKCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGTAT EU0 : CACTCATCAGGTGTTGTGCGCCCGGG : FmagUSCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGTAT EU0 : CACTCATCAGGTGTTGTGCGCCCGGG : FbusVNATCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGAAT EU0 : CACTTATCAAGTGTTGTGCGCCCGGT : 380 * 400 * 420 * 440 * FspNB-VN GGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCAC FspCB-VN GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACG : ACCGACGTTGCTGTCTGGCTGTTGCG : FgigSNTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC AY2 : CGACGTTGCTGTCTGGCTGTTGCGG : FgigTHTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC HM0 : CGACGTTGCTGTCTGGCTGTTGCGG : FgigKETACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC EU0 : : GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACG CGACGTTGCTGTCTGGCTGTTGCGG : FgET ACCGACGTTGCTGTCTGGCTGTTGCG : FgEQB GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACG : ACCGACGTTGCTGTCTGGCTGTTGCG : FhEQB GGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCA : CGACCGACGTTGCTGTCTGGCTGTTG : FspEQO GGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCAC : GACCGACGTTGCTGTCTGGCTGTTGC : FhAus : GGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCA CGACCGACGTTGCTGTCTGGCTGTTG : FhBe : CGGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACC ACGACCGACGTTGCTGTCTGGCTGTT : FhepEG- TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC EU0 : CGACGTTGCTGTCTGGCTGTTGCGG : FhepSATACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC AY2 : CGACGTTGCTGTCTGGCTGTTGCGG : FjacLKTATTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGAC EU0 : CGGCGTTGCCGTCTGGCTGTTGCGG : FmagUSTGTTGACGGACCTGCTTGCCAGTGCACTTTCTCGGAGTGTTCACCACGA EU0 : CCGGCGTTGCTGTCTGACTGTTGCGG : FbusVNTAATGACGAGCCCGCTCGCCAGTGCACTTTCTCAGAGTGTTCACCACGA EU0 : CCGGCGTTGCCGTCTGGCTGTTGCGG : 460 * 480 * 500 * 520 FspNB-VN GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGC GGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGG FspCB-VN : AGGTAGCCCGTTGTGCGATTTATCGT : FgigSNTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCA AY2 : GGTAGCCCGTTGTGCGATTTATCGTG : FgigTHTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCA HM0 : GGTAGCCCGTTGTGCGATTTATCGTG : FgigKETTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCA EU0 : : GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGC GGTAGCCCGTTGTGCGATTTATCGTG : FgET AGGTAGCCCGTTGTGCGATTTATCGT : FgEQB GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGC : AGGTAGCCCGTTGTGCGATTTATCGT : FhEQB CGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATG : GCAGGTAGCCCGTTGTGCGATTTATC : FspEQO GGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGG : CAGGTAGCCCGTTGTGCGATTTATCG : FhAus : CGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATG GCAGGTAGCCCGTTGTGCGATTTATC : FhBe : GCGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGAT GGCAGGTAACCCGTTGTGCGATTTAT : FhepEG- TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCA EU0 : GGTAGCCCGTTGTGCGATTTATCGTG : FhepSATTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCA AY2 : GGTAGCCCGTTGTGCGATTTATCGTG : FjacLKTTAAACCGGCCTGGTAGGGTCCTTGTGGCCTTGCTAGGTCGGGATGGCA EU0 : GGTAGCCCGTTGTGCGATTTATTGTG : FmagUSTTAAACCGGTTTGGTAGGGTCCTTGTGGCCTTGCTAGGTCGGGATGGCA EU0 : GGTAGCCCGTTGTGCGATTTATCGTG : FbusVNTTAAACCGGCCTGGTAGGGTCCTTGTGGCTCTGCTAGGTCGGGATGGCA EU0 : GGTAGCCTGTTGTACAATTTATTGTG : * 540 * 560 * 580 * 600 FspNB-VN GCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAG TGCTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGA FspCB-VN : ACGGCGGCTTGTTGTGCGTGCGGGCG : FgigSNCTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA AY2 : CGGCGGCTTGTTGTGCGTGCGGGCGT : FgigTHCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA HM0 : CGGCGGCTTGTTGTGCGTGCGGGCGT : FgigKECTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA EU0 : : GCTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAG CGGCGGCTTGTTGTGCGTGCGGGCGT : FgET ACGGCGGCTTGTTGTGCGTGCGGGCG : FgEQB GCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAG : ACGGCGGCTTGTTGTGCGTGCGGGCG : FhEQB GTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGG : AGACGGCGGCTTGTTGTGCGTGCGGG : FspEQO TGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGA : GACGGCGGCTTGTTGTGCGTGCGGGC : FhAus : GTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGG AGACGGCGGCTTGTTGTGCGTGCGGG : FhBe : CGTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCG GAGACGGCGGCTTGTTGTGCGTGCGG : FhepEG- CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA EU0 : CGGCGGCTTGTTGTGCGTGCGGGCGT : FhepSACTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA AY2 : CGGCGGCTTGTTGTGCGTGCGGGCGT : FjacLKCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGCCGGAGA EU0 : CGGCGGCTTGTTGTGCTTGCGGGCGT : FmagUSCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGA EU0 : CGGCGGCTTGTTGTGCGTGCGGGCGT : FbusVNCTCCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTGCGTCGGAGA EU0 : CGGCGGCTTGTTGTGCTTGCAGGCGT : * 620 * 640 * 660 * FspNB-VN GTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGA FspCB-VN TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT : GCAGGCCAGGTGATGGCTCGGTGTC : FgigSNGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTAY2 : AATGCAGGCCAGGTGATGGCTCGGTGTCG : FgigTHGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT HM0 : GCAGGCCAGGTGATGGCTCGGTGTCG : FgigKEGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT EU0 : : TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT GCAGGCCAGGTGATGGCTCGGTGTCG : FgET GCAGGCCAGGTGATGGCTCGGTGTC : FgEQB TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT : GCAGGCCAGGTGATGGCTCGGTGTC : FhEQB CGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTG : AATGCAGGCCAGGTGATGGCTCGGTG : FspEQO GTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGA : ATGCAGGCCAGGTGATGGCTCGGTGT : FhAus : CGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTG AATGCAGGCCAGGTGATGGCTCGGTG : FhBe : GCGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGT GAATGCAGGCCAGGTGATGGCTCGGT : FhepEG- GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT EU0 : : FhepSA- GCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT AY2 : GCAGGCCAGGTGATGGCTCGGTGTCG : FjacLKGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT EU0 : GCAGGCCAGGTGATGGCTCGGTGTCG : FmagUSGCCTGTCGCGTCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAAT EU0 : GCAGGCCAGGTGATGGCTCGGTGTCG : FbusVNGCCTGCTGTGCTGGCGGCTCTGAGTCTGGTTGCCTTGTTGCTTGTGAATA EU0 : CAAGCCAGGTGATGGCTTGGTGTCG : 680 * 700 * 720 * 740 * FspNB-VN : CGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCT : FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 : : : : GTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTG TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA : : : : 37 37 537 37 37 337 37 37 44 44 44 844 45 44 45 45 045 45 44 844 44 44 44 52 52 352 52 352 52 52 52 52 52 52 52 52 352 52 59 959 59 59 860 59 60 60 60 60 59 59 859 59 859 67 67 67 367 67 67 67 67 67 67 67 367 67 367 67 749 749 747 748 748 63 FgET : GTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTG FgEQB : TGGTGTAGTGGTAGACTATCCACCTG : FhEQB TCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTC : TGTGGTGTAGTGGTAGACTATCCACC : FspEQO CGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCT : GTGGTGTAGTGGTAGACTATCCACCT : FhAus : TCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTC TGTGGTGTAGTGGTAGACTATCCACC : FhBe : GTCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGT CTGTGGTGTAGTGGTAGACTATCCAC : FhepEG- TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGT EU0 : GGTGTAGTGGTAGACTATCCACCTGA : FhepSATTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGT AY2 : GGTGTAGTGGTAGACTATCCACCTGA : FjacLKTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGT EU0 : GGTGTAGTGGTAGACTATCCACCTGA : FmagUSTTCGGCCGGCGGTTGCGTCTGTTGCACTTTGAWAGGGCCCACAGTCTGT EU0 : GGTGTAGTGGTAGACTATCCACCTGA : FbusVNTTTGGTCGGCGGTTGCGTTTGTTGCACTTTAAAAGGGCCCATAGTCTGT EU0 : GGTGTAGTGGTAGACTATCCACCTGA : 760 * 780 * 800 * 820 FspNB-VN GACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCA FspCB-VN ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCAT : TGGGCGTTACGAAACCCAAAGGCGCAG : FgigSNCCCGTCTTGAAACACGGACCAAGGAGAGTAAACTGTGCGCGAGTCATT AY2 : GGGCGTTACGAAACCCAAAGGCGCAGT : FgigTHCCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT HM0 : GGGCGTTACGAAACCCAAAGGCGCAGT : FgigKECCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT EU0 : : ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCAT GGGCGTTACGAAACCCAAAGGCGCAGT : FgET TGGGCGTTACGAAACCCAAAGGCGCAG : FgEQB ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCAT : TGGGCGTTACGAAACCCAAAGGCGCAG : FhEQB TGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTC : ATTGGGCGTTACGAAACCCAAAGGCGC : FspEQO GACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCA : TTGGGCGTTACGAAACCCAAAGGCGCA : FhAus : TGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTC ATTGGGCGTTACGAAACCCAAAGGCGC : FhBe : CTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGT CATTGGGCGTTACGAAACCCAAAGGCG : FhepEG- CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT EU0 : GGGCGTTACGAAACCCAAAGGCGCAGT : FhepSACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT AY2 : GGGCGTTACGAAACCCAAAGGCGCAGT : FjacLKCCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT EU0 : GGGCGTTACGAAACCCAAAGGCGCAGT : FmagUSCCCGTCNNGAAACWCGGACCAAGGAGAGTAACATGTGCGCGAGTCAT EU0 : : FbusVN- TGGGCGTTACGAAACCCAAAGGCGAAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATT EU0 : GGGCGTTACGAAACCCAAAGGCGCAGT : * 840 * 860 * 880 * 900 FspNB-VN TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTT GTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGT FspCB-VN : CTCATGCGCGGTACTACCAAGCTTCG : FgigSNGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTC AY2 : TCATGCGCGGTACTACCAAGCTTCGA : FgigTHGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTC HM0 : TCATGCGCGGTACTACCAAGCTTCGA : FgigKEGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTC EU0 : : TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTT TCATGCGCGGTACTACCAAGCTTCGA : FgET CTCATGCGCGGTACTACCAAGCTTCG : FgEQB TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTT : TCTCATGCGCGGTACTACCAAGCTTCG : FhEQB AGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCG : TTTCTCATGCGCGGTACTACCAAGCTT : FspEQO GTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGT : TTCTCATGCGCGGTACTACCAAGCTTC : FhAus : AGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCG TTTCTCATGCGCGGTACTACCAAGCTT : FhBe : CAGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTC GTTTCTCATGCGCGGTACTACCAAGCT : FhepEG- GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTC EU0 : TCATGCGCGGTACTACCAAGCTTCGA : FhepSAGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTC AY2 : TCATGCGCGGTACTACCAAGCTTCGA : FjacLKGAAAGTAAAGGCTTGGCTTGTCTGAGCTGAGGTGAGATCCTGTCGTTTC EU0 : TCATGCGCGGTACCACCAAGCTTCGA : FmagUSGAAAGTAAAGGCTCGGCTTGTYTGAGCTGAGGWGAGATCCTGTCSTTT EU0 : CTCATGCCCGGTACTACCAAGCTTCGA : FbusVNGAAAGTAAAGGCTTGGCTTGTCTGAGCTGAGGTGAGATCCTGTCGTTTC EU0 : TCATGCGCGGTACTACCAAGCTTCGA : * 920 * 940 * 960 * FspNB-VN AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCG GAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTC FspCB-VN : TGCTTGCTCATCGTCGGGGCGGAGCAT : FgigSNGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT AY2 : GCTTGCTCATCGTCGGGGCGGAGCATG : FgigTHGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT HM0 : GCTTGCTCATCGTCGGGGCGGAGCATG : FgigKEGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT EU0 : : AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCG GCTTGCTCATCGTCGGGGCGGAGCATG : FgET TGCTTGCTCATCGTCGGGGCGGAGCAT : FgEQB AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCG : TGCTTGCTCATCGTCGGGGCGGAGCAT : FhEQB CGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCT : CGTGCTTGCTCATCGTCGGGGCGGAGC : FspEQO GAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTC : GTGCTTGCTCATCGTCGGGGCGGAGCA : FhAus : CGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCT CGTGCTTGCTCATCGTCGGGGCGGAGC : FhBe : TCGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCC TCGTGCTTGCTCATCGTCGGGGCGGAG : FhepEG- GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT EU0 : GCTTGCTCATCGTCGGGGCGGAGCATG : FhepSAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT AY2 : GCTTGCTCATCGTCGGGGCGGAGCATG : FjacLKGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCGGCCTCGA EU0 : GCTTGCTCATCGTCGGGGCGGAGCATG : FmagUSGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGT EU0 : GCTTGCTCATCGTCGGGGCGGAGCATG : FbusVNGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTGGAGGCAGCCTCGT EU0 : GCTTGCTCATCGTCGGGGCGGAGCATG : 980 * 1000 * 1020 * 1040 FspNB-VN : TGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA-: 1039 FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA ATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA-ATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA : : : : : : : : : : : : : : : 1038 1035 1036 1036 1039 1038 1041 1040 1041 1042 1036 1036 1036 1036 1036 75 74 75 75 75 75 74 74 874 74 74 82 82 82 382 82 82 82 82 82 82 82 382 82 82 382 89 89 789 89 890 89 90 90 90 090 89 89 89 889 89 97 497 97 97 397 97 97 97 597 97 97 97 397 97 97 64 Phụ lục Giản đồ giải trình tự nucleotide gan 28S FspCB-VN FspNB-VN 65 Phụ lục Trình tự nucleotide gen cox1 * 20 * 40 * 60 * FspNBGTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTG FspCB-VNGTGAATTATATTAGTTGATTGTTAACGTTAGATCATAAGCGTGTTGGTTTG c: ATTTATATGTTGATTGGTCTTTGG : 57 Fhep-AUGTGAAGTATATGAGTTGGTTGTTTACGTTGGATCATAAGCGTGTTGGTTT co : GATTTATATGGTTATTGGTCTTTGG : FspGHLGTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTG 75 CN- : ATTTATATGTTGATTGGTCTTTGG : Fgig-CNGTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTG co : ATTTATATGTTGATTGGTCTTTGG : Fgig-ID-co GTGAATTATATTAGTTGGTTGTTTACGTTAGATCATAAGCGTGTTGGTTTG : ATTTATATGTTGATTGGTCTTTGG : Fbus(hum GTGAGATATTTTGGTTGGTTGTTTACTTTAGATCATAAGCGTATTGGTTTT an : GTTTATTTGGTAGTTGGTTTGTGG : Fbus(swin GTGAGATATTTTGGTTGGTTGTTTACTTTAGATCATAAGCGTATTGGTTTT e: GTTTATTTGGTAGTTGGTTTGTGG : Csin-CNGTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTT co : ATATGATTCTTGGGATCTGGGGT : Csin-KRGTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTT co : ATATGATTCTTGGGATCTGGGGT : 57 Csin-RUGTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTT co : ATATGATTCTTGGTATCTGGGGT : Csin-VNGTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTT 75 co : ATATGATTCTTGGGATCTGGGGT : Oviv-THGTGTCTACGATATGACTTCTTACAATGGATCATAAGCGTATTGGTGTTATTT co : ACATGATTTTGGGGGTCTGAGGT : Oviv-CNGTGTCTACGATATGGCTTCTTACAATGGATCATAAGCGTATTGGTGTTATTT co : ACATGATTTTGGGGGTCTGAGGT : Ofel-RUGTGTCTGCGATATGGCTTTTTACTATGGATCATAAGCGTATTGGTATTATTT co : ATATGATTTTGGGTGTGTGAGGC : Phet-VNATGTTTACTTGGTTGTTTACTTTAGATCATAAGCGTATAGGTGTGATTTACA co : TGGTCTTGGGTGTGTGGTGTGGA : Pohi-JP-co : ATGACTTGATTATTTACTTTGGATCATAAGCGTATTGGTATGATTTACATGG Pwes(2n)ATGTTTACTTGGTTGTTCACTTTGGATCACAAGCGTATCGGGATGATTTAC K: ATGGTTTTGGGGATTTGGGGAGGA : 57 Pwes(3n) ATGTTTACTTGGTTGTTCACTTTGGATCACAAGCGTATCGGGATGATTTAC KR : ATGGTTTTGGGGATTTGGGGAGGA : Ejap-VNATGATGTGGATGACTTGGTTATTTACTTTAGATCATAAGCGAATTGGTTTA 75 co : ATTTATATGTTGATTGGGGTATGA : Htai-KRATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATAT co : ATGATTGTTGGAGTGTGAGGGGGC : Htai-LA-co ATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATAT : ATGATTGTTGGAGTGTGAGGGGGC : Htai-VNATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATAT co : ATGATTGTTGGAGTGTGAGGGGGC : Sfal-VN-co GTGTTGCTCTGGTTTTTTACGCTGGATCATAAGCGTGTTGGTATGATTTAT : TTTCTTTTGGGTGTATGGGGAGGC : 80 * 100 * 120 * 140 * FspNBFspCB-VN- GGGGGATTTTTTGGTCTTTCTTTGAGTGTTTTGGTTCGTTTGAATTATTT GGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTG 15 c: GATCCTTATTTTAATTTGGTGTCC : Fhep-AUGGGGGGTTTTTTGGTCTTTCTTTGAGGGTGTTGGTTCGTTTGAATTATTT 15 co : AGATCCATATTTTAATTTGGTTTCT : FspGHLGGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTG 15 CN- : GATCCTTATTTTAATTTGGTGTCC : Fgig-CNGGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTG 15 co : GATCCTTATTTTAATTTGGTGTCT : Fgig-ID-co GGGGGATTTTTTGGTCTTTCTTTGAGTGTTTTGGTTCGTTTGAATTATTT 15 : GGATCCTTATTTTAATTTGGTGTCC : Fbus(hum AGAGGGTTTTTGGGTTTGTCGTTGAGTATGTTGATTCGTTTAAAATATTT 15 an : GGATCCTTATTATAATTTGATTTCT : Fbus(swin AGAGGGTTTTTGGGTTTGTCGTTGAGTATGTTGATTCGTTTAAAATATTT 15 e: GGATCCTTATTATAATTTGATTTCT : Csin-CNGGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGA 15 co : TCCTTACTTTAATGTTATTTCATCA : 015 Csin-KRGGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGA co : TCCTTACTTTAATGTTATTTCATCA : Csin-RUGGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGA 15 co : TCCTTACTTTAATGTTATTTCATCA : Csin-VNGGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGA 15 co : TCCTTACTTTAATGTTATTTCATCA : Oviv-THGGATTTTTGGGCCTGGGGCTTAGGTTTCTTATACGAGTTAATTATTTTGA 15 co : TCCTTATTTTAATATTGTTTCTCCG : Oviv-CNGGATTTTTGGGCCTGGGGCTTAGGTTTCTTATACGAGTTAATTATTTTGAT 15 co : CCTTATTTTAATATTGTTTCTCCG : Ofel-RUGGGTTTACAGGATTGAGGTTGAGGTTTCTTATACGGATAAATTATTTTGA 15 co : TCCCTATTTTAATGTTGTTTCTCCC : 014 Phet-VNTTTTTGGGGCTCTCTTTAAGGATGTTGATTCGCTTGAACTAco : CTTGGATCCTTACTACAATATTGTTTCGTCGGA : Pohi-JP-co TTTATTGGACTTTCTTTAAGAATGTTAATTCGTTTGAATTATCTTG14 : ATCCGTATTATAATATTGTCTCGTCGGA : Pwes(2n)TTTCTGGGTCTTTCTCTGAGGATGTTGATTCGTCTGAACTA14 K: CCTGGATCCTTACTACAACATTGTCTCGTCGGA : Pwes(3n) TTTCTGGGTCTTTCTCTGAGGATGTTGATTCGTCTGAACTA14 KR : CCTGGATCCTTACTACAACATTGTCTCGTCGGA : Ejap-VN- GGGGGTTTTTTGGGTCTTTCTTTGAGTTTGTTGATACGTTTGAAATATTT 15 co : GGATCCTTATTTTAATTTGATAATA : Htai-KR- TTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCT 15 co : TACTATAATGTTGTATCCCCAGAG : Htai-LA-co TTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCT 15 : TACTATAATGTTGTATCCCCAGAG : Htai-VNTTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCT 15 co : TACTATAATGTTGTATCCCCAGAG : Sfal-VN-co TTTTTGGGGCTTTCTTTGAGCTTTCTTATTCGGGTAAAATACTTGGATCCT 15 : TATTTTAAAGTCGTTTCGTCGGAT : 160 * 180 * 200 * 220 FspNBFspCB-VNCCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTT 22 c: TTCTTTTTGATGCCTGTGTTGATT : Fhep-AUCCTGAAGTTTATAATTATGTTGTTACTGGGCATGGGATTATAATGATTTTT 22 co : TTCTTTTTGATGCCGGTGTTGATT : FspGHLCCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTT 22 CN- : TTCTTTTTGATGCCTGTGTTGATT : Fgig-CNCCTGAGGTTTATAATTATGTTGTGACGGGGCATGGTGTTATTATGATTTTT 22 co : TTCTTTTTAATGCCTGTGTTGATT : Fgig-ID-co CCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTT 22 : TTCTTTTTGATGCCTGTGTTGATT : Fbus(hum CCGGAGGTGTATAATTATTTGGTTACAGGTCATGGTATAGTGATGATTTTT 22 an : TTTTTTCTGATGCCTGTGTTGATA : Fbus(swin CCGGAGGTGTATAATTATTTGGTTACAGGTCATGGTATAGTGATGATTTTT 22 e: TTTTTTCTGATGCCTGTGTTGATA : 522 Csin-CNGATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTC co : TTCTTGATGCCTGTTCTTGTGGGC : Csin-KRGATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTC 22 co : TTCTTGATGCCTGTTCTTGTGGGC : Csin-RUGATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTC 22 co : TTCTTGATGCCTGTTCTTGTGGGC : Csin-VNGATGTTAATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTC 22 co : TTCTTGATGCCTGTTCTTGTGGGC : Oviv-THGATGTTTATAATTATATTGTTACGAGTCATGGGATAGCTATGATCTTTTTCT 22 co : TTTTAATGCCTGTTCTTATTGGT : Oviv-CNGGTGTTTATAATTATATTGTTACGAGTCATGGGATAGCTATGATCTTTTTCT 22 co : TTTTAATGCCTGTTCTTATTGGT : Ofel-RUGATGTATATAATTACGTTGCTACTAGTCATGGGGTTGCCATGATTTTTTTTT 22 co : TCTTAATGCCGGTTTTAATAGGG : Phet-VNGGTTTACAATTACGTGATAACGAATCATGGTGTTGCTATGATTTTTTTCTT 22 co : TTTGATGCCTGTCTTGATAGGCGG : 422 Pohi-JP-co GGTTTACAATTATGTGATAACTAATCATGGGGTTGCTATGATTTTCTTTTT : TTTGATGCCTGTGTTGATAGGAGG : Pwes(2n)GGTTTACAACTATGTGCTGACCAATCATGGGGTCGCCATGATTTTCTTTTT 22 K: CCTGATGCCTGTTTTGATAGGGGG : Pwes(3n) GGTTTACAACTATGTGCTGACCAATCATGGGGTCGCCATGATTTTCTTTTT 22 KR : CCTGATGCCTGTTTTGATAGGGGG : Ejap-VNCCTGAGGTATATAATTATGTGATTACTTGACATGGGATAGTGATGATTTTC 22 co : TTTTTTTTGATGCCTGTTTTGATA : Htai-KRGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCT 22 co : TAATGCCGGTTTTGATTGGGGGG : Htai-LA-co GTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCT 22 : TAATGCCGGTTTTGATTGGGGGG : Htai-VNGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCT 22 co : TAATGCCGGTTTTGATTGGGGGG : Sfal-VN-co GTTTACAACTACATAATTACAAGGCATGGGATAGCGATGATTTTCTTTTTC 22 : TTGATGCCCGTGTTAATTGGTAGG : * 240 * 260 * 280 * 300 FspNB-VN-c : GGGGGGTTTGGTAaTTATTTAATGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG : 300 FspCB-VN-c : GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTACCTCGTTTAAATGCTTTG : Fhep-AU-co : GGGGGTTTTGGTAATTATTTGTTGCCTTTATTGTTAGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG : FspGHL-CN- : GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTACCTCGTTTAAATGCTTTG : 300 300 300 66 Fgig-CN- GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTG Fgig-ID-co : AATTTGCCTCGTTTAAATGCTTTG : Fbus(hum GGTGGTTTTGGTAATTATTTGTTACCGTTGTTGTTAGGGATAGGTGATTT an : GAATTTACCTCGTGTAAATGCTTTG : Fbus(swin GGTGGTTTTGGTAATTATTTGTTACCGTTGTTGTTAGGGATAGGTGATTT e: GAATTTACCTCGTGTAAATGCTTTG : Csin-CNGGTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAAT co : TTGCCTCGTTTGAAAGCTCTAAGC : Csin-KRGGTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAAT co : TTGCCTCGTTTGAAAGCTCTAAGC : Csin-RUGGTTTTGGTAACTACCTACTGCCTCTGTTACTTGGTTTATCAGATTTGAAT co : TTGCCTCGTTTGAAAGCTCTAAGC : Csin-VNGTTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAAT co : TTGCCTCGTTTGAAAGCTCTAAGT : Oviv-THGGGTTTGGTAATTATTTATTGCCCTTGTTGTTGGGTTTATCGGACTTGAAT co : TTGCCCCGTTTAAAAGCGTTGAGT : Oviv-CNGGGTTTGGTATTTATTTATTGCCCTTGTTGTTGGGTTTATCGGACTTGAAT co : TTGCCCCGTTTAAAAGCGTTGAGT : Ofel-RUGGTTTTGGTAAATATTTGTTGCCTCTGTTGCTGGGTTTGTCGGATTTAAAT co : TTACCCCGTTTGAAAGCTTTAAGT : Phet-VNGTTTGGTAATTATCTTCTTCCGTTGTTGTTAGGTATTCCTGATCTGAAACTT co : CCTCGTTTGAAAGCTCTTAGGGC : Pohi-JP-co ATTCGGTAATTATCTTCTTCCTCTTTTATTGGGTATACCTGATTTGAATCTTC : CTCGTTTGAAAGCTCTTAGGGC : Pwes(2n)GTTTGGTAATTACCTCCTCCCTCTTTTGCTAGGAATTCCTGATCTGAGGTT K: ACCTCGCTTGAAAGCTCTTAGGGC : Pwes(3n) GTTTGGTAATTACCTCCTCCCTCTTTTGCTAGGAATTCCTGATCTGAGGTT KR : ACCTCGCTTGAAAGCTCTTAGGGC : Ejap-VNGGGGGGTTTGGTAAATACTTGTTACCTTTATTGTTAGGATTACCTGATTT co : GAATTTGCCTCGTTTAAAAGCTTTA : Htai-KRTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTA co : CCCCGTTTAAATGCCCTTAGAGCT : Htai-LA-co TTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTA : CCCCGTTTAAATGCCCTTAGAGCT : Htai-VNTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTA co : CCCCGTTTAAATGCCCTTAGAGCT : Sfal-VN-co TTTGGTAATTATTTGTTGCCGCTGCTGTTGGGGTTGCCGGATCTTACTTTG : CCTCGGTTAAAAGCCTTTAGTGCC : * 320 * 340 * 360 * FspNBAGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATG FspCB-VNc: GGGGGTATGGGTGTTGCTTGGACT : Fhep-AUAGTGCTTGGTTGTTATTTCCTGCTTGTGTTTGTTTGCCTTTTGGTTTGATA co : GGGGGTGTAGGTGTTGGTTGAACT : FspGHLAGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATG CN- : GGGGGTATGGGTGTTGGTTGGACT : Fgig-CNAGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATG co : : Fgig-ID-co GGGGGTATGGGTGTTGGTTGGACT AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATG :Fbus(hum GGGGGTATGGGTGTTGGTTGGACT : AGAGTGTGGTTGTTATTGCCATCCTGCGTTTGTTTGGCTTTGAGTTTGAT an : AGGGGGTGCTGGTGTTGGTTGAACT : Fbus(swin AGAGTGTGGTTGTTATTGCCATCCTGCGTTTGTTTGGCTTTGAGTTTGAT e: AGGGGGTGCTGGTGTTGGTTGAACT : Csin-CNGTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGT co : GGTGCTGGGGTTGGGTGGACTTTT : Csin-KRGTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGT co : GGTGCTGGGGTTGGGTGGACTTTT : Csin-RUGTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGT co : GGTGCTGGGGTTGGGTGGACTTTT : Csin-VNGTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGT co : GGTGCTGGGGTTGGGTGGACTTTT : Oviv-THGTTTGGTTGTTGGTTCCTTCCGCCGTCTGTTTTAGTCTGAGGATGTATGG co : TGGAGCTGGTGTAGGTTGAACTTTT : Oviv-CNGTTTGGTTGTTGGTTCCTTCCGCCGTCTGTTTTAGTCTGAGGATGTATGG co : TGGGGCTGGTGTAGGTTGAACTTTT : Ofel-RUGTGTGGTTATTGGTTCCTTCGGCTTTATGCTTTAGGTTGAGAATGTATGGT co : GGGGCTGGAGTTGGGTGAACTTTT : Phet-VNTTGATTAATGCTTCCTTCGGCGGTTTGCCTTAGTGTGAGAATGTTAGGAG co : GTGCAGGAGTGGGGTGGACCTTTTA : Pohi-JP-co TTGATTAATGCTTCCTTCTGCTGTTTGTCTTAGTTTGAGCATGCTAGGAGG : GGCAGGAGTAGGATGAACCTTCTA : Pwes(2n)TTGGTTGATGCTTCCTTCTGCTGTCTGTCTCAGTTTGAGGATGTTAGGTG K: GTGCAGGTGTGGGTTGGACTTTTTA : Pwes(3n) TTGGTTGATGCTTCCTTCTGCTGTCTGTCTCAGTTTGAGGATGTTAGGTG KR : GTGCAGGTGTGGGTTGGACTTTTTA : Ejap-VNAGTGCTTGATTGTTGTTACCTTCTGCGGTTTGTTTGGGGATGAGGATGAT co : TGGGGGTGCTGGAGTAGGTTGGACA : Htai-KRTGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGG co : GTCTGGGGTTGGCTGGACTTTTTAT : Htai-LA-co TGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGG : GTCTGGGGTTGGCTGGACTTTTTAT : Htai-VNTGGTTAATGATTCCTTCTGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGG co : GTCTGGGGTTGGCTGGACTTTTTAT : Sfal-VN-co TGATTGATGGTTCCTGCGTCTATCAGGTTTGGTTTGAGGATGATAGGAG : GATCGGGGGTTGGTTGGACTTTTTAT : 380 * 400 * 420 * 440 * FspNBTTCTATCCTCCTCTTTCTAGATTGGATTATTCTGGTTGGGGGGTTGATTTT FspCB-VNTTCTATCCTCCTCTTTCTAGATTGGATTTTTCTGGTTGGGGAGTTGATTTT c: TTAATGTTTTCTCTTCATTTGGCT : Fhep-AUTTTTATCCACCTCTTTCTAGGTTGGATTATTCTAGGTGGGGGGTTGATTTT co : TTGATGTTTTCTCTTCATTTGGCT : FspGHLTTCTATCCTCCTCTTTCTAGATTGGATTTTTCTGGTTGGGGAGTTGATTTT CN- : TTAATGTTTTCTCTTCATTTGGCT : Fgig-CNTTTTATCCCCCTCTTTCTAGTTTGGATTATTCTGGTTGGGGGGTTGATTTT co : TTAATGTTTTCTCTTCATTTGGCT : Fgig-ID-co TTCTATCCTCCTCTTTCTAGATTGGATTATTCTGGTTGGGGGGTTGATTTT : TTAATGTTTTCCCTTCATTTGGCT : Fbus(hum TTTTATCCTCCTCTGTCTGCTTCAGGTTATTCTGGTTGGGGGGTGGATTTT an : ATGATGTTTTCTTTGCATTTGGCC : Fbus(swin TTTTATCCTCCTCTGTCTGCTTCAGGTTATTCTGGTTGGGGGGTGGATTTT e: ATGATGTTTTCTTTGCATTTGGCC : Csin-CNTATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTT co : AATGTTTTCGCTTCATTTGGCTGGG : Csin-KRTATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTA co : ATGTTTTCGCTTCATTTGGCTGGG : Csin-RUTATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTA co : ATGTTTTCGCTTCATTTGGCTGGG : Csin-VNTATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTA co : ATGTTTTCGCTTCATTTGGCTGGG : Oviv-THTATCCCCCTCTATCTGCTGGTGATTATAGGGGCTGGGGCGTTGACTTTCTG co : ATGTTTTCCCTTCATTTGGCTGGG : Oviv-CNTATCCCCCTCTATCTGCTGGTGATTATAGGGGCTGGGGCGTTGATTTTCTG co : ATGTTTTCCCTTCATTTGGCTGGG : Ofel-RUTATCCTCCCCTGTCTTCTGGTGATTATAGGGGGTGGGGAGTTGATTTTTT co : GATGTTTTCTCTTCATTTGGCTGGA : Phet-VNTCCCCCTCTTTCGGGTGGTGATTATTCTGGTTGGGGTACGGATTTTTTGAT co : GTTTTCTCTTCATTTGGCTGGTCT : Pohi-JP-co TCCGCCTTTATCAGGTGGGGATTATTCTGGGTGAGGTACAGATTTTTTGA : TGTTTTCTCTTCATTTGGCGGGGTT : Pwes(2n)TCCTCCTCTCTCGGGAGGTGATTACTCTGGCTGGGGTGTGGATTTTTTGA K: TGTTCTCTCTTCATCTGGCTGGTCT : Pwes(3n) TCCTCCTCTCTCGGGAGGTGATTACTCTGGCTGGGGTGTGGATTTTTTGA KR : TGTTCTCTCTTCATCTGGCTGGTCT : Ejap-VNTTTTATCCTCCTCTTTCTGGAGTAGGTTATTCAgGTTGGGGTGTGGATTTT co : TTAATGTTTGCTTTACATTTGGCT : Htai-KRCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTAT co : GTTTTCTTTACATTTGGCGGGGTTA : Htai-LA-co CCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTAT : GTTTTCTTTACATTTGGCGGGGTTA : Htai-VNCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTAT co : GTTTTCTTTACATTTGGCGGGGTTA : Sfal-VN-co CCTCCTCTTTCTTCGGGTGATTACAGAGGATGGGGAGTGGATTTTCTTAT : GTTTTCTCTGCATCTTGCTGGGCTG : 460 * 480 * 500 * 520 FspNB-VN-c : GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGCGAGGGTATTGGT : FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co : : : : : : : : : : : : : GGTGTTTCTAGTCTTTTGCGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGGGTTTCTAGTCTTTTAGGTTCTATTAAATTTATTTGTACTATTTTAGAGGTTATGTTGGATGAGGGTACGGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGCGAGGGTACTGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGGGTATCTAGTATTTTTAGTTCTATTAAATTTATTTGTACTATAGTTGAGGTTATGTTTGAGGAAGGTACAGGT GGGGTATCTAGTATTTTTAGTTCTATTAAATTTATTTGTACTATAGTTGAGGTTATGTTTGAGGAAGGTACAGGT TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTCGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCTAGTCTTTTTGGGTCCTTGAAATTTATTTGTACTATATGGTCAGCAATGGATAGTCGGATTTTGGAGCGT TTATCTAGTCTTTTTGGGTCCTTGAAATTTATTTGTACTATATGGTCAGCAATGGATAGTCGGATTTTGGAGCGT : : : : : : : : : : : : : 30 30 030 30 30 30 30 30 30 30 029 29 29 29 30 30 30 30 030 37 37 37 37 37 537 37 37 37 37 37 37 37 537 37 37 37 37 37 37 37 537 37 45 45 45 45 045 45 45 45 45 45 45 45 045 45 44 44 44 44 45 45 045 45 45 525 525 525 525 525 525 525 525 525 525 525 525 525 525 67 Ofel-RU- TTCTAGTCTTTTTGGTTCGTTGAAATTTATTTGTACTATCGTCAGGGCGTT TTGTCTAGTCTTTTTGGGTCTTTGAATTTTATCTGTACTATTTGGTCTGCCA Phet-VNco : GTGTGAGCATACTGTGGGTCGGAG : Pohi-JP-co ATCTAGTCTTTTGGGTTCCTTAAATTTTATTTGTACTATTGTTAGGGCGTTA : TGCGAGCATACGGTGGGTCGTAG : Pwes(2n)CTCGAGCCTTCTTGGTTCCTTGAAATTCATTTGCACCATTGTTAGGGCGTT K: GTGTGAGCACACTGTGGGTCGCAG : Pwes(3n) CTCGAGCCTTCTTGGTTCCTTGAAATTCATTTGCACCATTGTTAGGGCGTT KR : GTGTGAGCACACTGTGGGTCGCAG : Ejap-VNGGTGTCTCTAGTTTACTTGGTTCAATAaATTTTATTTGTACAATTGTGGAG co : GTGATGTTGGAGGAGAGTACTGCT : Htai-KRTCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATG co : AGTAATGATACCAGGGAGCGGTAC : Htai-LA-co TCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATG : AGTAATGATACCAGGGAGCGGTAC : Htai-VNTCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATG co : AGTAATGATACCAGGGAGCGGTAC : Sfal-VN-co TCTAGGGTGTTTGGGTCACTGAATTTTGTTTGTACTATCCTGGAGGTGAT : GCTGGGAAGGATGAGCGAGCGGTAT : * 540 * 560 * 580 * 600 FspNBCGTCATAGTATTTTAGTTTGGGCTTATTTGTTTACTTCTATTTTGTTGTTATT FspCB-VN- CGTCATAGTATTTTAGTTCGGGCTTATTTGTTTACTTCTATTTTGTTGTTATT c: GTCTTTGCCGGTTTTAGCTGCT : Fhep-AUCGGCATAGTATTTTGGTTTGGGCTTATCTATTTACTTCTGTTTTATTGTTGT co : TGTCATTGCCTGTTTTGGCTGCT : FspGHLCGTCATAGTATTTTAGTTCGGGCTTATTTGTTTACTTCTATTTTGTTGTTATT CN- : GTCTTTGCCGGTTTTAGCTGCT : Fgig-CNCGTCATAGTATTTTAGTTTGGGCTTATTTGTTTACTTCTATTTTGTTGTTGT co : TGTCTTTGCCGGTTTTAGCTGCT : Fgig-ID-co CGTCATAGTATTTTAGTTTGGGCTTATTCGTTTACTTCTATTTTGTTGTTATT : GTCTTTGCCGGTTTTAGCTGCT : Fbus(hum CGTTTGAGTATTTTGGTTTGGGCTTATTTGTTTACTTCTATTTTATTGCTTT an : TATCTTTGCCTGTATTAGCTGCT : Fbus(swin CGTTTGAGTATTTTGGTTTGGGCTTATTTGTTTACTTCTATTTTATTGCTTT e: TATCTTTGCCTGTATTAGCTGCT : Csin-CNCACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTA co : CGTTGCCTGTTTTAGCTGCTGGT : Csin-KRCACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTA co : CGTTGCCTGTTTTAGCTGCTGGT : Csin-RUCACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTA co : CGTTGCCTGTTTTAGCTGCTGGT : Csin-VNCACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTA co : CGTTGCCTGTTTTAGCTGCTGGT : Oviv-THCATTCAATTATAGTGTGGGCTTATATGTTTACTTCAATTTTGTTGCTGGGT co : ACGTTGCCCGTTTTGGCGGCTGGT : Oviv-CNCATTCAATTATAGTGTGGGCTTATATGTTTACTTCAATTTTGTTGCTGGGT co : ACGTTGCCCGTTTTGGCGGCTGGT : Ofel-RUCATTCTATCATAATTTGGGCTTATCTATTTACTTCTTTTTTGTTGTTGGGTA co : : Phet-VN- CATTGCCTGTTTTGGCGGCTGCT GTCTATAATTGTTTGGGCCTATCTTTTTACGTCTATCTTGTTGCTTCTTTCTT co : : Pohi-JP-co TGCCGGTGCTTGCTGCTGCGAT GTCTATAATTGTTTGGGCGTATCTTTTTACGTCTGTTTTGTTACTACTTTCT : TTACCAGTATTAGCGGCTGCAAT : Pwes(2n)GTCAATAATTGTCTGGGCTTATCTCTTCACCTCTGTTCTGCTGCTCCTCTCT K: TTACCTGTGCTTGCTGCTGCGAT : Pwes(3n) GTCAATAATTGTCTGGGCTTATCTCTTCACCTCTGTTCTGCTGCTCCTCTCT KR : TTACCTGTGCTTGCTGCTGCGAT : Ejap-VNCGTCATAGGGTGTTGGTTTGAGGGTATTTGTTCACGTCGATCTTGTTGTT co : GCTtTCTTTGCCTGtATTAGCGGCT : Htai-KRTCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGTT co : ACCAGTGCTAGCTGCGGGTATA : Htai-LA-co TCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGTT : ACCAGTGCTAGCTGCGGGTATA : Htai-VNTCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGT co : TACCAGTGCTAGCTGCGGGTATA : Sfal-VN-co TCAATCATTGTTTGGGCGTATCTTTTTACTTCAATTTTGCTTTTGTTGTCGT : TGCCTGTGTTGGCTGCGGGTATT : * 620 * 640 * 660 * FspNBFspCB-VNGCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATC c: GTATGGGGGGTGGTGATCCTGTC : Fhep-AUGCTATTACTATGTTGTTATTTGATCGTAAATTTGGTTCTGCTTTTTTTGATC co : CTATGGGAGGTGGGGATCCTGTT : FspGHLGCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATC CN- : CTATGGGGGGTGGTGATCCTGTC : Fgig-CNGCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATC co : CTATGGGGGGTGGCGATCCTGTC : Fgig-ID-co GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATC : CTATGGGGGGTGGCGATCCTGTC : Fbus(hum GCTATAACTATGTTATTGTTTGATCGTAGTTTTGGGTCTGCATTTTTTGATC an : CTATGGGGGGGGGAGATCCGGTG : Fbus(swin GCTATAACTATGTTATTGTTTGATCGTAGTTTTGGGTCTGCATTTTTTGATC e: CTATGGGGGGGGGAGATCCGGTG : Csin-CNATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGT co : CGGGTGGGGGTGATCCTTTGTTG : Csin-KRATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGT co : CGGGTGGGGGTGATCCTTTGTTG : Csin-RUATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCG co : TCGGGTGGGGGTGATCCTTTGTTG : Csin-VNATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGT co : CGGGTGGGGGTGATCCTTTGTTG : Oviv-THATAACTATGCTTCTTTTTGATCGTAAATTTGGTACTGTGTTTTTTGATCCGT co : CTGGGGGTGGTGATCCGTTGTTG : Oviv-CNATAACTATGCTTCTTTTTGATCGTAAATTTGGTACTGTGTTTTTTGATCCGT co : CTGGGGGTGGTGATCCGTTGTTG : Ofel-RUATAACCATGCTTCTTTTTGATCGTAATTTTGGTACTGTGTTTTTTGATCCAT co : CCGGTGGGGGTGATCCTGTGTTA : Phet-VNTACCATGCTTTTGTTTGATCGAAAATTTAGTTCTGCTTTTTTTGACCCCCT co : TGGTGGTGGTGATCCTGTTTTATT : Pohi-JP-co TACCATGCTTTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCCTTG : GGTGGAGGTGATCCTGTTTTGTT : Pwes(2n)CACCATGCTTTTGTTCGATCGGAAATTTAGTTCTGCCTTTTTTGATCCGTT K: GGGGGGTGGAGATCCCGTTTTGTT : Pwes(3n) CACCATGCTTTTGTTCGATCGGAAATTTAGTTCTGCCTTTTTTGATCCGTT KR : GGGGGGTGGAGATCCCGTTTTGTT : Ejap-VNGCTATTACGATGTTGTTGTTTGATCGTAAATTTGGGGCTTCATTTTTTGAT co : CCGATGGGAGGTGGGGATCCTGTG : Htai-KRACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTG co : GGAGGGGGGGACCCTGTCTTGTTC : Htai-LA-co ACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTG : GGAGGGGGGGACCCTGTCTTGTTC : Htai-VNACGATGCTTTTGTTTGATCGTAGATTTAGCTCTTCTTTTTTTGATCCATTG co : GGAGGGGGGGACCCTGTCTTGTTC : Sfal-VN-co ACCATGCTTCTTTTTGATCGTAACTTTGGTTCTGCCTTTTTTGATCCTTTG : GGTGGGGGAGACCCCGTTTTGTTC : 680 * 700 * 720 * 740 * FspNB-VN-c : TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT : FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co : : : : : : : : : : : : : : : : : : : : : : TTATTTCAGCATTTGTTTTGGTTTATTGGTCATCCGGAAGTTTATGCTTTGATTTTGCCTGGGTTTGGAGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTGATTTTACCCGGGTTTGGGGTTATT TTATTTCAGCATTTGTTTTGGTTTATTGGTCATCCGGAAGTTTATGCTTTGATTTTGCCTGGGTTTGGAGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT TTGTTTCAGCATTTGTTTTGGTTTTTTGGGCATCCCGAGGTGTATGTTTTGATTTTGCCAGGGTTTGGTGTGATT TTGTTTCAGCATTTGTTTTGGTTTTTTGGGCATCCCGAGGTGTATGTTTTGATTTTGCCAGGGTTTGGTGTGATT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAACATATGTTTTGGTTTTTCGGTCATCCGGAAGTATACGTGTTGATTTTGCCAGGATTCGGTATGATTAGT TTTCAACATATGTTTTGGTTTTTCGGTCATCCGGAAGTATACGTGTTGATTTTGCCAGGATTCGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCGGAGGTGTATGTGTTAATATTGCCGGGGTTTGGAATGATTAGT TCAACACCTTTTTTGGTTTTTTGGCCATCCGGAGGTGTATGTTTTAATTTTGCCTGGATTTGGTGTTGTGAGACA CCAGCATTTGTTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTAATTTTACCAGGATTTGGGATTGTGAGACA TCAGCATTTGTTTTGGTTCTTTGGGCATCCGGAGGTGTATGTCCTGATTTTGCCTGGGTTTGGTATCGTGAGCCA TCAGCATTTGTTTTGGTTCTTTGGGCATCCGGAGGTGTATGTCCTGATTTTGCCTGGGTTTGGTATCGTGAGCCA TTGTTTCAGCATTTATTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTAATTTTGCCTGCTTTTGGAGTAATT CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGGGTTTGGTATAGTGAGACAC CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGGGTTTGGTATAGTGAGACAC CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATTCTCCCAGGGTTTGGTATAGTGAGACAC : : : : : : : : : : : : : : : : : : : : : : 52 52 52 52 52 552 52 52 52 60 60 060 60 60 60 60 60 60 60 060 60 60 60 59 59 59 59 960 60 60 60 60 67 567 67 67 67 67 67 67 67 567 67 67 67 67 67 67 67 467 67 67 67 67 67 750 750 750 750 750 750 750 750 750 750 750 750 750 750 750 749 746 749 749 750 750 750 750 68 Sfal-VN-co : CAGCATATGTTTTGATTCTTTGGTCATCCCGAGGTTTACGTTTTGATTCTCCCTGGGTTTGGTATTG TTAGTCAT : 750 760 * 780 * 800 * 820 FspNBAGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATG FspCB-VN- AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATG 82 c: GTCTTATTTTGGCTATGGCTGCT : Fhep-AUAGTCATATTTGTGTGACTTTAACTAATAATGATTCTTTGTTTGGTTATTATG 82 co : GTCTTATTTTAGCTATGGCTGCT : FspGHLAGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATG 82 CN- : GTCTTATTTTGGCTATGGCTGCT : Fgig-CNAGCCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATG 82 co : GTCTTATTTTGGCTATGGCTGCT : Fgig-ID-co AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATG 82 : GTCTTATTTTGGCTATGGCTGCT : Fbus(hum AGACATATTTGTGTTACTTTGACTAATAAAGATTCTTTGTTTGGTTATTAT 82 an : GGGCTTGTTTTGGCTATGGCTGCT : Fbus(swin AGACATATTTGTGTTACTTTGACTAATAAAGATTCTTTGTTTGGTTATTAT 82 e: GGGCTTGTTTTGGCTATGGCTGCT : 582 Csin-CNCACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGG co : CTTGGTGTTGGCTATGTTTGCTGTA : Csin-KRCACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGG 82 co : CTTGGTGTTGGCTATGTTTGCTGTA : Csin-RUCACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGG 82 co : CTTGGTGTTGGCTATGTTTGCTATA : Csin-VNCACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGG 82 co : CTTGGTGTTGGCTATGTTTGCTATA : Oviv-THCATATCTGTACTACGTTAACTGGTAATGATTCTTTGTTCGGTTATGGGGGT 82 co : TTGGTGTTGGCCATGTTTGCTATA : Oviv-CNCATATCTGTACTACGTTAACTGGTAATGATTCTTTGTTCGGTTATGGTGGT 82 co : TTGGTGTTGGCCATGTTTGCTATA : Ofel-RUCATGTTTGTACGACTCTAACAGGGAAAGATTCCCTATTTGGTTATGGTGG 82 co : TTTGGTGTTAGCCATGTTTGCTATA : Phet-VNTATCTGCATGACTTTGACTAATAAAGATTCTTTGTTTGGTTATTATGGCTT 82 co : GGTTTTTGCTATGGGGGCTATTGT : 482 Pohi-JP-co TATTTGTATGACTCTAACTAATAAAGATTCGTTGTTTGGTTATTATGGATT : GGTGTTTGCTATGGGGGCGATTGT : Pwes(2n)CATTTGCATGACCCTGACCAACAACGATTCCTTGTTCGGGTACTACGGGC 82 K: TGGTGTTTGCGATGGGGGCCATTGT : Pwes(3n) CATTTGCATGACCCTGACCAACAACGATTCCTTGTTCGGGTACTACGGGC 82 KR : TGGTGTTTGCGATGGGGGCCATTGT : Ejap-VNAGGCATATTTGTGTAACGCTGACTAATAAAGATTCTTTATTTGGTTATTAT 82 co : GGTTTGGTGTTAGCTATGGCGGCT : Htai-KRATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTT 82 co : AGTTCTTGCTATGTTTTCTATAGTC : Htai-LA-co ATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTT 82 : AGTTCTTGCTATGTTTTCTATAGTC : Htai-VNATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTT 82 co : AGTTCTTGCTATGTTTTCTATAGTC : Sfal-VN-co GTTTGCATGACTCTAACGAACAATGATTCCTTGTTTGGTTACGGGGGTCT 82 : GGTCCTTGCTATGTTTTCTATTGTC : * 840 * 860 * 880 * 900 FspNBFspCB-VN- ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGT 90 c: TTGGATGTACATACTGCTGTTTTT : Fhep-AUATAGTATGTTTAGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGT 90 co : TTGGATGTGCATACTGCTGTTTTT : FspGHLATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGT 90 CN- : TTGGATGTACATACTGCTGTTTTT : Fgig-CNATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGT 90 co : TTGGATGTACATACTGCTGTTTTT : Fgig-ID-co ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGT 90 : TTGGATGTACATACTGCTGTTTTT : Fbus(hum ATAGTTTGTTTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGGT 90 an : TTGGATGTGCATACTGCTGTTTTT : 090 Fbus(swin ATAGTTTGTTTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGGT e: TTGGATGTGCATACTGCTGTTTTT : Csin-CNGTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCT 90 co : GGATTTGGGGACTGCTGTTTTTTTT : Csin-KRGTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCT 90 co : GGATTTGGGGACTGCTGTTTTTTTT : Csin-RUGTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCT 90 co : GGATTTGGGGACTGCTGTTTTTTTT : Csin-VNGTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCT 90 co : GGATTTGGGGACTGCTGTTTTTTTT : Oviv-THGTCTGTTTAGGAAGGGTGGTTTGGGCTCATCATATGTTTACGGTTGGGCT 90 co : GGATTTGGGCACCGCTGTGTTTTTT : Oviv-CNGTCTGTTTAGGAAGGGTGGTTTGGGCTCATCATATGTTTACGGTTGGGCT 90 co : GGATTTGGGCACCGCTGTGTTTTTT : Ofel-RUGTTTGTTTGGGTAGTGTGGTTTGAGCTCATCATATGTTTACTGTAGGATTA 90 co : GATTTAGGGACTGCTATTTTTTTT : 089 Phet-VNGTGTTTGGGGAGGGTTGTTTGAGCGCACCACATGTTTATGGTTGGTTTA co : GATGTTAAGACTGCTGTTTTTTTTAG : Pohi-JP-co GTGTTTGGGAAGTGTTGTCTGGGCCCACCATATGTTTATGGTTGGTTTGG 89 : ATGTTAAGACTGCAGTATTTTTCAG : Pwes(2n)GTGTCTGGGTAGTGTTGTGTGGGCGCATCACATGTTCATGGTTGGTTTG 89 K: GATGTCAAGACTGCTGTTTTCTTTAG : Pwes(3n) GTGTCTGGGTAGTGTTGTGTGGGCGCATCACATGTTCATGGTTGGTTTG 89 KR : GATGTCAAGACTGCTGTTTTCTTTAG : Ejap-VNATTGTGTGTCTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGG 90 co : GTTGGATATAGATACCGCGGTGTTT : Htai-KRTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGA 90 co : TGTTAAGACGGCTGTTTTTTTTAGT : Htai-LA-co TGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGA 90 : TGTTAAGACGGCTGTTTTTTTTAGT : Htai-VNTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGA 90 co : TGTTAAGACGGCTGTTTTTTTTAGT : 090 Sfal-VN-co TGTCTGGGGAGTATTGTTTGGGCACATCATATGTTTATGGTAGGTCTGGA : TTTGAAGACTGCGGTATTTTTTAGG : * 920 * 940 * 960 * FspNBFspCB-VN- TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGGATTAAGGTTTTT TTTAGTTCTGTTACTATGGTTATTGGTATCCCTACCGGGATTAAGGTTTTT 97 c: TCTTGGTTAATAATGTTGGGGGGT : Fhep-AUTTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGTATTAAGGTCTTTT 97 co : CCTGGTTGATAATGTTGGGGGGG : FspGHLTTTAGTTCTGTTACTATGGTTATTGGTATCCCTACCGGGATTAAGGTTTTTT 97 CN- : CTTGGTTAATAATGTTGGGGGGT : Fgig-CNTTTAGTTCTGTTACTATGGTTATTGGTATTCCTACTGGGATTAAGGTTTTCT 97 co : CTTGATTAATAATGTTGGGGGGT : Fgig-ID-co TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGGATTAAGGTTTTT 97 : TCTTGGTTAATAATGTTGGGGGGT : 597 Fbus(hum TTTAGTTCTGTTACTATGGTGATTAGTATTCCCACAGGTATTAAGGTTTTTT an : CTTGGTTGATAATGTTAGGGGGG : Fbus(swin TTTAGTTCTGTTACTATGGTGATTAGTATTCCCACAGGTATTAAGGTTTTTT 97 e: CTTGGTTGATAATGTTAGGGGGG : Csin-CNAGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTC 97 co : ATGATTATATATGCTTGCTGGAACT : Csin-KRAGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTC 97 co : ATGATTATATATGCTTGCTGGAACT : Csin-RUAGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTC 97 co : ATGATTATATATGCTTGCTGGAACT : Csin-VNAGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTC 97 co : ATGATTATATATGCTTGCTGGAACT : Oviv-THAGCTCGGTTACTATGATTATAGGGGTTCCTACTGGAATAAAGGTTTTTTC 97 co : TTGGTTGTATATGCTTGCGGGTACT : Oviv-CNAGCTCGGTTACTATGATTATAGGGGTTCCTACTGGAATAAAGGTTTTTTC 97 co : TTGGTTGTATATGCTTGCGGGTACT : 597 Ofel-RUAGTTCAGTTACTATGATCATTGGTGTACCTACAGGGATAAAGGTTTTTTCT co : TGATTATACATGCTTGCCGGTACT : Phet-VNTTCTGTTACTGGGGTGATTGGGATTCCCACAGGGATTAAGGTTTTTTCTT 97 co : GGTTGTTTATGTTGGGGGGGACTCG : Pohi-JP-co ATCTGTTACGGGGGTGATAGGTATTCCTACAGGGATTAAGGTTTTTTCTT 97 : GGTTATTTATGTTAGGGGGTACTCG : Pwes(2n)TTCTGTCACGGGAGTGATTGGGATACCCACGGGGATTAAGGTTTTCTCTT 97 K: GGTTGTTCATGCTGGGTGGAACTCG : Pwes(3n) TTCTGTCACGGGAGTGATTGGGATACCCACGGGGATTAAGGTTTTCTCTT 97 KR : GGTTGTTCATGCTGGGTGGAACTCG : Ejap-VNTTTAGGTCTGTGACGATGGTTATTGGTATCCCTACAGGCATTAAGGTTTTT 97 co : TCTTGGTTGGTTATGTTAAGTGGT : Htai-KRTCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTG 97 co : GCTGTATATGTTGGCGGGAAGTCGG : Htai-LA-co TCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTG 97 : GCTGTATATGTTGGCGGGAAGTCGG : 597 Htai-VNTCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTG co : GCTGTATATGTTGGCGGGAAGTCGG : Sfal-VN-co TCTGTAACTATGATTATCGGTGTTCCTACAGGTATTAAGGTGTTTTCGTGG 97 : TTGTATATGTTGGCTGGAAGCCGT : 980 * 1000 * 1020 * 1040 * FspNB-VN-c : GGTGGTTCTGTTCGTATTTGGGATCCTGTTATATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG : 1050 FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human : : : : : : GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGTTCTGTTCGTATATGGGATCCTGTTGTGTGGTGAATTATAGGGTTTATTGTTTTATTTACTATTGGTGGG GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTGGTTCTGTTCGTATTTGGGATCCTGTTATATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGATTTGTTCGTATTTGGGATCCCATAGTGTGATGAATTGTGGGGTTTGTTGTGTTGTTTACTATAGGGGGG : : : : : : 1050 1050 1050 1050 1050 1050 69 Fbus(swin CGGGAGCGTCTATGAGACCCAATCATGTGGTGGATAATCGGGTTTGTGG GGTAGATTTGTTCGTATTTGGGATCCCATAGTGTGATGAATTGTGGGGTT 105 Csin-CNco : TGCTTTTCACTATAGGCGGGGTTACT : Csin-KRCGGGAGCGTCTATGAGACCCAATCATGTGGTGGATAATCGGGTTTGTGG 105 co : TGCTTTTCACTATAGGCGGGGTTACT : 0105 Csin-RUCGGGAGCGTCTATGAGATCCAATCATGTGGTGGATAATCGGGTTTGTGG co : TGCTTTTCACTATAGGCGGGGTTACT : Csin-VNCGGGAGCGTCTATGAGATCCAATCATGTGGTGGATAATCGGGTTTGTGG 105 co : TGCTTTTCACTATAGGCGGGGTTACT : Oviv-THCGTGAGCGTTTTTGGGACCCGATTATGTGGTGGATAGTTGGGTTCGTAG 105 co : TACTTTTTACTATAGGGGGGGTTACC : 0105 Oviv-CNCGTGAGCGTTTTTGGGACCCGATTATGTGGTGGATAGTTGGGTTCGTAG co : TACTTTTTACTATAGGGGGGGTTACC : Ofel-RUCGAGATCGTCTTTGGGATCCGATTATGTGGTGGATAATCGGATTTATAGT 105 co : GCTTTTTACTATAGGTGGGGTTACT : Phet-VNTTTACGGTTTTGAGATCCGGTGGTTTGGTGAATTTTAGGCTTTATTTTTC 104 co : TTTTTACTATTGGTGGTGTAACTGG : Pohi-JP-co TCTACGGTTTTGGGATCCTGTAATATGATGAATTCTTGGGTTTATCTTTTT 104 : GTTTACAATAGGGGGTGTAACTGG : Pwes(2n)TTTGCGGTTTTGGGATCCTGTTTTGTGGTGAATTCTTGGGTTTATCTTCCT 104 K: GTTCACCATAGGTGGTGTGACCGG : 9104 Pwes(3n) TTTGCGGTTTTGGGATCCTGTTTTGTGGTGAATTCTTGGGTTTATCTTCCT KR : GTTCACCATAGGTGGTGTGACCGG : Ejap-VNAGGATGTCTCGTTTAGGGGACCCTATAATTTGGTGGATTGTGGGGTTTAT 105 co : AGGATTGTTCACTATAGGGGGAGTG : Htai-KRGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTT 105 co : ATTTACTATCGGGGGGGTGACCGGG : 0105 Htai-LA-co GGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTT : ATTTACTATCGGGGGGGTGACCGGG : Htai-VNGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTT 105 co : ATTTACTATCGGGGGGGTGACCGGG : Sfal-VN-co GGCCGTTTTTGAGATCCGGTGATGTGGTGGATACTGGGATTCATCGTCT 105 : TGTTTACCATCGGGGGTATTACGGGT : 1060 * 1080 * 1100 * 1120 FspNBFspCB-VN- GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATA 112 c: CGTGGTTTGTGGTTGCTCATTTT : Fhep-AUGTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACTTTGCTTCATGATA 112 co : CATGGTTTGTGGTTGCTCATTTT : FspGHLGTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATA 112 CN- : CGTGGTTTGTGGTTGCTCATTTT : 5112 Fgig-CNGTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATA co : CGTGGTTTGTGGTTGCTCATTTT : Fgig-ID-co GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATA 112 : CGTGGTTTGTGGTTGCTCATTTT : Fbus(hum GTTACTGGTATTATGCTTTCTGCTTCTATTTTGGATACTTTGTTGCATGATA 112 an : CTTGGTTTGTTGTGGCTCATTTC : Fbus(swin GTTACTGGTATTATGCTTTCTGCTTCTATTTTGGATACTTTGTTGCATGAT 112 e: ACTTGGTTTGTTGTGGCTCATTTC : Csin-CNGGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGG 112 co : TTTGTTATTGCTCATTTTCATTAT : 5112 Csin-KRGGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGG co : TTTGTTATTGCTCATTTTCATTAT : Csin-RUGGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGG 112 co : TTTGTTATTGCTCATTTTCATTAT : Csin-VNGGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGG 112 co : TTTGTTATTGCTCATTTTCATTAT : 5112 Oviv-THGGTATAGTTCTTTCTGCTTCGGTTATAGATGCTTTATTTCATGATACTTGA co : TTTGTTATAGCTCATTTTCATTAT : Oviv-CNGGTATAGTTCTTTCTGCTTCGGTTATAGATGCTTTATTTCATGATACTTGAT 112 co : TTGTTATAGCTCATTTTCATTAT : Ofel-RUGGGATAGTTCTTTCTGCCTCTGTGATTGATGCTCTTTTTCATGATACATGA 112 co : TTCGTTATTGCCCACTTTCATTAT : Phet-VNGATTATTTTGTCTTCTTCTATTTTGGATAGTCTGTTACATGATACCTGGTTT 112 co : GTTGTTGCTCACTTTCACTATGT : Pohi-JP-co AATTATCTTGTCTTCTTCTATTTTGGACAGTTTATTGCATGACACTTGGTT 112 : TGTTGTTGCACATTTCCATTATGT : 1112 Pwes(2n)CATCATTCTGTCCTCCTCCATACTGGATAGTCTGTTGCATGATACGTGGTT K : CGTCGTGGCTCATTTTCATTACGT : Pwes(3n) CATCATTCTGTCCTCCTCCATACTGGATAGTCTGTTGCATGATACGTGGTT 112 KR : CGTCGTGGCTCATTTTCATTACGT : Ejap-VN- ACTGGGATTATGTTGTCTGCTTCTATATTGGATACTTTGTTGCATGATACT 112 co : TGGTTTGTGGTGGCCCATTTTCAT : 5112 Htai-KRATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTT co : GTAATAGCTCACTTTCATTATGTT : Htai-LA-co ATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTT 112 : GTAATAGCTCACTTTCATTATGTT : Htai-VNATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTT 112 co : GTAATAGCTCATTTTCATTATGTT : Sfal-VN-co GTAGTGCTTTCGTCTTCTGTGATGGATACTTTGATGCACGATACTTGATTT 112 : GTTATTGCTCATTTTCATTACGTT : * 1140 * 1160 * 1180 * 1200 FspNBFspCB-VNCATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTG 120 c: GTGGTGGCCTATTGTTACGGGT : Fhep-AUCATTATGTTCTTTCTTTAGGGTCTTATAGAAGTGTTGTTATCTCTTTTATTT 120 co : GGTGGTGGCCTGTTGTGACTGGT : 0120 FspGHLCATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTG CN- : GTGGTGGCCTATTGTTACGGGT : Fgig-CNCATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTG 120 co : GTGGTGGCCTATTGTTACGGGT : Fgig-ID-co CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTG 120 : GTGGTGGCCTATTGTTACGGGT : 0120 Fbus(hum CATTATGTTCTTTCATTAGGTTCTTATAGGAGTGTTGTTATATCTTTTATTT an : GATGGTGGCCAATAATTACTGAT : Fbus(swin CATTATGTTCTTTCATTAGGTTCTTATAGGAGTGTTGTTATATCTTTTATTT 120 e: GATGGTGGCCAATAATTACTGAT : Csin-CNGTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTG 120 co : ATGACCTGTGTTTACGGGGTATAGC : Csin-KRGTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTG 120 co : ATGACCTGTGTTTACGGGGTATAGC : Csin-RUGTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTG 120 co : ATGACCTGTGTTTACGGGGTATAGC : 0120 Csin-VNGTTTTATCTTTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTG co : ATGACCTGTGTTTACGGGGTATAGC : Oviv-THGTGCTGTCATTAGGATCCTTTAGAACGGTGGTTATATCCGTTGTTTGGTG 120 co : GTGGCCTGTTTTTACTGGTTACAGT : Oviv-CNGTGCTGTCATTAGGATCCTTTAGAACGGTGGTTATATCCGTTGTTTGGTG 120 co : GTGGCCTGTTTTTACTGGTTATAGT : 0120 Ofel-RUGTTTTATCCTTGGGATCTTTTAGTACTGTTGTTATATCAATGATTTGGTGG co : TGGCCTGTGTTTACCGGGTTTAGC : Phet-VNTCTATCTTTGGGATCTTATAGAACTGTGGTGATATCCCTCTTGTGATGATG 119 co : GCCGGTTGTCACCGGGTTTAGCTT : 9119 Pohi-JP-co TTTATCCCTGGGATCTTATAGCACTGTTGTAATATCCCTTCTGTGATGGTG : GCCTATTGTGACCGGGTTTAGTTT : Pwes(2n)CTTGTCTCTGGGGTCCTACAGGACCGTAGTGATATCCCTGTTGTGGTGGT 119 K: GGCCCGTTGTTACGGGGTTCAGTTT : 9119 Pwes(3n) CTTGTCTCTGGGGTCCTACAGGACCGTAGTGATATCCCTGTTGTGGTGGT KR : GGCCCGTTGTTACGGGGTTCAGTTT : Ejap-VNTATGTTTTATCCTTGGGTTCATATAGTGGTGTTGTGATTTCGTTTTTGTGG 120 co : TGGTGGCCTATTATAACGGGGTAT : Htai-KRTTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGG 120 co : CCTGTGATAGTGGGTTATAGGTTG : Htai-LA-co TTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGG 120 : CCTGTGATAGTGGGTTATAGGTTG : 0120 Htai-VNttatcgTTAGGTTCTTATATGTCgGTGGTGATATCTATGATTTGATGATGGCC co : TGTGATAGTGGGTTATAGGTTG : Sfal-VN-co TTGTCTTTAGGATCGTACAGTACTGTTGTAATCTCGTTGATCTGGTGGTG 120 : GCCTGCAGTTGTTGGGTCGAGACTT : * 1220 * 1240 * 1260 * FspNB-VN-c : TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT : 1275 FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co : : : : : : : : : : : : : : : : TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGTTTGAATCTTTTCATGTTGCAAGGTCATTGGGTTGTTTCTATGATTGGGTTTAATATGTGTTTTTTCCCT TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TACAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGTTTAAAAGTTTCTTTGTTGCAAGGTCATTGGATTGTTTCTATGTTTGGTTTTAATTTGTGTTTTTTTCCG TATAGTTTAAAAGTTTCTTTGTTGCAAGGTCATTGGATTGTTTCTATGTTTGGTTTTAATTTGTGTTTTTTTCCG TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATTTATTAATGGCTCATTGAGGTGTCTCAATGTTAGGGTTCAATCTGTGTTTCTTTCCAATGCAC TTAAATAAGTATTTATTAATGGCTCATTGAGGTGTCTCAATGTTAGGGTTCAATCTATGTTTCTTTCCAATGCAC CTTAACAAGTATTTGTTAGTGGCTCATTGAGGTGTGTCAATGATAGGGTTTAATCTTTGTTTCTTCCCAATGCAT GAATAAGTATTTGTTGCAGGGTCATTGGGTGGCATCTATGGTGGGGTTTAATCTGTGTTTTTTTCCCATGCATTA AAATAAGTATTTGTTGCAAGGGCATTGGGTGGCATCAATGGTAGGGTTTAATTTTTGTTTTTTCCCCATGCATTA : : : : : : : : : : : : : : : : 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1274 1271 70 Pwes(2n)- GAATAAGTACCTGTTGCAGGGTCATTGGGTGAGGTCGATGCTGGGGTT GAATAAGTACCTGTTGCAGGGTCATTGGGTGAGGTCGATGCTGGGGTTT 127 Pwes(3n) KR : TAACCTTTGTTTTTTTCCGATGCATTA : Ejap-VNAGGTTAAATCGTTTATTATTGTGGGGACATTGAATTGTTTCGATGATAGG 127 co : GTTTAATTTGTGTTTTTTCCCTATG : Htai-KRAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCA 127 co : ATTTGTGTTTTTTTCCTATGCATTTT : 5127 Htai-LA-co AAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCA : ATTTGTGTTTTTTTCCTATGCATTTT : Htai-VNAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTTA 127 co : ATTTGTGTTTTTTTCCTATGCATTTT : 5127 Sfal-VN-co AAAAAGTATTTATTGATGGGCCATTGGGGTGTGTCGATGATAGGTTTTA : ACTTGTGCTTTTTCCCGATGCACTTT : 1280 * 1300 * 1320 * 1340 * FspNBFspCB-VNATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGAT 135 c: CCTGATTTTTATTGGCTTAGTGTT : Fhep-AUATGCACTACCTTGGTATGGCGGGGTTGCCTCGCCGGGTTTGTGTTTATGA 135 co : TCCGGATTTTTATTGGCTTAGTGTT : FspGHLATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGAT 135 CN- : CCTGATTTTTATTGGCTTAGTGTT : 0135 Fgig-CNATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGAT co : CCTGATTTTTATTGGCTTAGTGTT : Fgig-ID-co ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGAT 135 : CCTGATTTTTATTGGCTTAGTGTT : 0135 Fbus(hum ATGCATTATTTGGGTATGTCTGGGCTTCCTCGCCGGGTGTGTGTTTATGA an : TCCTGATTTTTATTGACTTAATGTG : Fbus(swin ATGCATTATTTGGGTATGTCTGGGCTTCCTCGCCGGGTGTGTGTTTATGA 135 e: TCCTGATTTTTATTGACTTAATGTG : Csin-CNTTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTTGTATGAGCCCTG 135 co : CCTTCAGTGGTTGAATACTTTGTGT : 0135 Csin-KRTTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTTGTATGAGCCCTG co : CCTTCAGTGGTTGAATACTTTGTGT : Csin-RUTTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTCGTATGAGCCTTG 135 co : CTTTCAGTGGTTGAATACTTTGTGT : Csin-VNTTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTCGTATGAGCCCTG 135 co : CTTTCAGTGGTTGAATACTTTGTGT : 0135 Oviv-THTTTTTAGGTATTTACGGTCTTCCACGTCGTGTGTGTTCTTATGAGCCTTGT co : TTTCAGTGGGTGAATACTGTGTGT : Oviv-CNTTTTTAGGTATTTACGGTCTTCCACGTCGCGTGTGTTCTTACGAGCCTTGT 135 co : TTTCAGTGGGTGAATACTGTGTGT : 0135 Ofel-RUTTTTTAGGCGTTTATGGACTACCGCGTCGGGTGTGTTCATATGAGCCTTG co : TTTTCAGTGGATAAATACTTTGTGT : Phet-VNCCTTGGGGCTTATGGTCTTCCTCGGCGGGTGTGTAACTATGAGCCTGCTT 134 co : TTCAGTGGCTAAATAATGTTTCTTC : Pohi-JP-co TCTTGGGGCTTATGGTCTTCCACGTCGGGTGTGTAACTATGAGCCGGCCT 134 : TTCAGTGATTAAACAATATTTCTTC : 6134 Pwes(2n)CCTCGGAGCTTACGGTCTCCCACGTCGGGTGTGTAACTATGAGCCCGCC K: TTTCAGTGGTTGAACAAAATCTCCTC : Pwes(3n) CCTCGGAGCTTACGGTCTCCCACGTCGGGTGTGTAACTATGAGCCCGCC 134 KR : TTTCAGTGGTTGAACAAAATCTCCTC : Ejap-VNCATTATTTTGGGTTGCAGGGGTTACCACGTCGTGTTTGTGTTTTTGATCC 135 co : TGAGTTTTGTTGGTTGAATTTTATT : 0135 Htai-KRTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTT co : TCAGTGGTTGAAAACTATGGCTAGG : Htai-LA-co TTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTT 135 : TCAGTGGTTGAAAACTATGGCTAGG : 0135 Htai-VNTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTT co : TCAGTGGTTGAAAACTATGGCTAGG : Sfal-VN-co TTAGGGTTGCATGGTCTTCCTCGTCGGGTGTGTGTTTATGAGCCGGCCTT 135 : TCAGTGGTTGAATAGTATATCAACT : 1360 * 1380 * 1400 * 1420 FspNBFspCB-VN- GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTG 142 cFhep-AU: TTTGTTTTGTGGGAGTCTTTTGTT : GTTTCTAGTTTAGGTGCTCTTGTCTCGGCAGGTAGTGCCTTTTTGTTTGT 142 co : GTTTATTTTGTGGGAGTCCTTTGTT : FspGHLGTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTG 142 CN- : TTTGTTTTGTGGGAGTCTTTTGTT : Fgig-CNGTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTG 142 co : TTTGTTTTGTGGGAGTCTTTTGTT : 5142 Fgig-ID-co GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTG : TTTGTTTTGTGGGAGTCTTTTGTT : Fbus(hum TTATCAAGTTTAGGGGGTGTGGTATCAACTGTAAGGGCCTTTTTTCTTTT 142 an : TTTTGTTTTATGGGAGTCTTGTGTG : Fbus(swin TTATCAAGTTTAGGGGGTGTGGTATCAACTGTAAGGGCCTTTTTTCTTTT 142 e: TTTTGTTTTATGGGAGTCTTGTGTG : Csin-CNAGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATAT 142 co : TGTTTGAGAATCTATGGCTGTTTGT : Csin-KRAGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATAT 142 co : TGTTTGAGAATCTATGGCTGTTTGT : Csin-RUAGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATAT 142 co : TGTTTGAGAATCTATGGCTGTTTGT : Csin-VNAGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATAT 142 co : TGTTTGAGAATCTATGGCTGTTTGT : Oviv-THAGAGTTGGTGGTATCTTTTCTGTGTTTAGTGGTTTTACCCTGATATACGTT 142 co : ATTTGGGAGTCTATGGCGGTGCGT : 5142 Oviv-CNAGAGTTGGTGGTATCTTTTCTGTGTTTAGTGGTTTTACCCTGATATACGTT co : ATTTGGGAGTCTATGGCGGTGCGT : Ofel-RUAGTATAGGTGGGATATTCTCGGTTTTTAGGGGGTTTACTCTTATGTATATA 142 co : GTTTGGGAGTCGATAGCTGTGAGT : Phet-VNTGTTGGTGCTTTAGTTTCTGTGGTTAGGGCCTTTTTTTTGGTTTTTATTCT 142 co : ATGGGAGTCTTTGGTTGTGGGTAA : 4142 Pohi-JP-co TGTTGGAGCTTTAATTTCTGTGGTTAGGGCTTTTTTTTTGGTTTTTATTCT : TTGAGAGTCTCTTGTGGTTGGAAA : Pwes(2n)TGTGGGGGCGCTGGTTTCTGTTGTCAGTGCTTTTTTTTTGGTTTTTATCC 142 K: TTTGGGAGTCTCTCGTTGTTGGGAA : 4142 Pwes(3n) TGTGGGGGCGCTGGTTTCTGTTGTCAGTGCTTTTTTTTTGGTTTTTATCC KR : TTTGGGAGTCTCTCGTTGTTGGGAA : Ejap-VNTGTAGGTTGGGCGGTATTCTTTCTGCAAGGAGTGCTTTTTTtTTTGTATAT 142 co : ATTTTGTGGGAGTCTTATACTGTG : Htai-KRTTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTT 142 co : ATGAGAGTCGTTGTCTGTGGGTAAT : 5142 Htai-LA-co TTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTT : ATGAGAGTCGTTGTCTGTGGGTAAT : Htai-VNCTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTT 142 co : ATGAGAGTCGTTGTCTGTGGGTAAT : Sfal-VN-co ATGGGGGGTCTGATTTCTGTGTTCAGCGGATTTGTTTTGTTGTATATACT 142 : CTGGGAGTCGTTGGTTGTGGGTAAT : * 1440 * 1460 * 1480 * 1500 FspNB-VN-c : GTTGGTAATCGTGTTGTTTCGTCTTGAGGTAACAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG : 1500 FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : GTTGGTAATTGTGTTGTTTCGTCTTGAGGTAGCAGTTCGTTAGTATTGAATGTTATTACTTTGCCTGGCCCTCAG GTAGGTAATTGTGTTGTCTCTTCTTGAGGTAGCAGGTCTTTAGTGTTGAATGTTATTACTTTGCCAGGTCCTCAA GTTGGTAATTGTGTTGTTTCGTCTTGAGGTAGCAGTTCGTTAGTATTGAATGTTATTACTTTGCCTGGCCCTCAG GTCGGTAATCGTGTTGTTTCGTCTTGAGGTAACAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG GTTGGTAATCGTGTTGTTTCGTCTTGAGGTAATAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG ATAGGTAATCGGGTACTTTCTGTTTGGGGTTCCGGTTCTTTGATTTTGAATGTTGTTACTTTACCTAGACCTCAG ATAGGTAATCGGGTACTTTCTGTTTGGGGTTCCGGTTCTTTGATTTTGAATGTTGTTACTTTACCTAGACCTCAG AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCACATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AATCGTGTAGTTAAATTGTGAGGGGGGGCTCATGTTGTGTTTAATGTGTTAGTTAATCCCGTTGGGATGCATAGC AATCGTGTAGTTAAATTGTGAGGGGGGGCTCATGTTGTGTTTAATGTGTTAGTTAATCCCGTTGGGATGCATAGC AATCGTGTAGTTCATTTGTGGGGTGGAGCCCATGTTGTGTTTAACGTTTTGGTTAATCCAATTGGGGCACATAGT TCGTGTTGTTGGTGTTTGGGGTACTAAAGCCCTGGTTATGAATGCCTTAGTTACTCCAGTTCCCCATCACAGTGT TCGTGTTGTTGGGGTTTGAGGTAGCAAATCTTTGGTTATGAATGCTTTGGTTACTCCTGTGCCTCATCATAGGGT TCGTGTCGTGGGTATCTGGGGTACGAATGCTTTAGTGGTGAATGCGTTGGTGACGCCTGTGCCGCATCACAGGAT TCGTGTCGTGGGTATCTGGGGTACGAATGCTTTAGTGGTGAATGCGTTGGTGACGCCTGTGCCGCATCACAGGAT AAGAAAGTGGTAGTGTCTTGTTGGGGTAGAAATTCTTTGGTGCTTAAAGTAGTTACTTTACCTCTACCACATCAT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTVCCGCATCATTGTAGT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTVCCGCATCATTGTAGT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTGCCGCATCATTGTAGT CGGGTTATTGGGCTATGGGGAAGTAATGGGTTGATTTTAAATGTTGTTACCCTGCCAGTACCCCATCATTTTAGT : : : : : : : : : : : : : : : : : : : : : : : 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1499 1496 1499 1499 1500 1500 1500 1500 1500 71 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 1520 * 1540 * 1560 CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGTTGGTCGCTGGGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAAAGGTTATATGACTGGGGGTTGTCGGTGGTGTGCTTAG CATAAAGGTTATATGACTGGGGGTTGTCGGTGGTGTGCTTAG CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA TCTTACTTATATTATCCTGTTTGTTGACTTCCCGCCGGGGGCCGTGTTTAG -TCTTACTTATATTATCCTGTTTGTTGACTTCCCGCCGGGGGCCGTGTTTAG -TCTTATTTACATTATCCCATTTGTTGGATGCCTAGAGGGGGCCGGCGATTGGAGGGGGTGTAG TTATATCAGTGGGCCGTCTCGTTGGCTTTCTTTTTAG -TTACATTAGTGGGCCGTCTCGTTGGCTTTCTTTTTAG -TTACATCAGTGGGCCGTCGCGGTGGCTTTCTTTTTAG -TTACATCAGTGGGCCGTCGCGGTGGCTTTCTTTTTAG -GGGGATTATATTCATACTTCAATACATTGGGTGTTTTAG -TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATGTGGGTGAGCCGTGCACTTGGCTTAAGGGTGTGTCGGTTTAG : : : : : : : : : : : : : : : : : : : : : : : : 1542 1542 1542 1542 1542 1542 1542 1542 1560 1560 1560 1560 1551 1551 1563 1536 1533 1536 1536 1539 1542 1542 1542 1545 ... thể COX1 loài sán gan lớn Fasciola sp dạng dạng lai gây bệnh động vật Việt Nam Mục têu đề tài - Giải trình tự phân tích gen nhân ITS- 2, gen 28S sán gan lớn dạng xác định dạng laigây bệnh động vậttại... trình tự hệ gen nhân vùng ITS- 2, 28S COX1 F hepatica F gigantica (thuần lai) Ý nghĩa khoa học thực tiễn - Góp phần cung cấp trình tự chuỗi gen ITS- 2, 28S cox1 sán gan lớn Fasciola sp Việt Nam - Ngồi... lồi Fasciola thực phương pháp sinh học phân tử dựa thị gen nhân ty thể Xuất phát từ u cầu đó, chúng tơi tiến hành thực đề tài: Nghiên cứu biến đổi thành phần gen nhân ITS- 2, 28S rRNA gen ty thể